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Isolation and Characterization of 13 Microsatellite Loci from Pedicularis rex (lousewort)
Huang, Pan-Hui1,2; Yu, Wen-Bin1,2; Yang, Jun-Bo3; Wang, Hong1; Lu, Lu1
2010-07-01
发表期刊HORTSCIENCE
卷号45期号:7页码:1129-1131
摘要Pedicularis rex C. B. Clarke ex Maxim., an endemic species with potential horticultural traits from Himalaya, has a unique cup-like petiole structure and highly infraspecific floral variation among members of the lousewort genus (Orobanchaceae). We developed 13 microsatellite markers from three microsatellite-enriched libraries (AG, AC, and AAG) of P. rex with a modified biotin-streptavidin capture technique. Polymorphism of each locus was assessed in 22 individuals with representation of five populations of P. rex. Number of alleles per locus (A) ranged from two to seven with an average of 4.38. The observed and expected heterozygosities varied from 0.03 to 0.86 and 0.45 to 0.84, respectively. Additionally, among the 13 identified microsatellite markers, 11 of them were successfully amplified in species P. thamnophila, and five of them showed polymorphisms. This study may provide important information for further investigation on the population genetics, introduction, and acclimatization of P. rex and its congeners.
资助信息National Natural Science Foundation of China [30970201, 30570115]; National Basic Research Program of China [2007CB411600]
学科领域Horticulture
收录类别SCI
语种英语
WOS研究方向Agriculture
WOS类目Horticulture
WOS记录号WOS:000280041000025
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文献类型期刊论文
条目标识符http://ir.kib.ac.cn/handle/151853/14111
专题中国西南野生生物种质资源库
作者单位1.Chinese Acad Sci, Key Lab Biodivers & Biogeog, Kunming Inst Bot, Kunming 650204, Yunnan, Peoples R China
2.Chinese Acad Sci, Grad Sch, Beijing 100049, Peoples R China
3.Chinese Acad Sci, Plant Germplasm & Genom Ctr, Germplasm Bank Wild Species, Kunming Inst Bot, Kunming 650204, Yunnan, Peoples R China
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Huang, Pan-Hui,Yu, Wen-Bin,Yang, Jun-Bo,et al. Isolation and Characterization of 13 Microsatellite Loci from Pedicularis rex (lousewort)[J]. HORTSCIENCE,2010,45(7):1129-1131.
APA Huang, Pan-Hui,Yu, Wen-Bin,Yang, Jun-Bo,Wang, Hong,&Lu, Lu.(2010).Isolation and Characterization of 13 Microsatellite Loci from Pedicularis rex (lousewort).HORTSCIENCE,45(7),1129-1131.
MLA Huang, Pan-Hui,et al."Isolation and Characterization of 13 Microsatellite Loci from Pedicularis rex (lousewort)".HORTSCIENCE 45.7(2010):1129-1131.
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