Isolation and Characterization of 13 Microsatellite Loci from Luculia pinceana (Rubiaceae), a Typical Distylous Species

	Isolation and Characterization of 13 Microsatellite Loci from Luculia pinceana (Rubiaceae), a Typical Distylous Species
	Zhou, Wei 1; Wang, Hong1 ; Li, De-Zhu1,2 ; Yang, Jun-Bo 2; Zhou, Wei 3
	2010-05-01
发表期刊	HORTSCIENCE
卷号	45 期号:5 页码:840-841
摘要	Luculia pinceana Hook. (Rubiaceae) is a typical distylous species with dimorphic and long-styled monomorphic populations. Within this study, we developed 13 microsatellite markers from L. pinceana using a modified biotin streptavidin capture method. Polymorphism of each locus was assessed in 30 individuals from four dimorphic populations and one monomorphic population. The average allele number of these microsatellites was 4.153 per locus ranging from three to seven. The observed and expected heterozygosities were from 0.040 to 0.840 and from 0.571 to 0.769, respectively. Additionally, all 13 identified microsatellite markers were successfully amplified in its related species, L. yunnanensis, 10 of which showed polymorphism. These microsatellite markers could provide a useful tool for further study of the breeding system and the population genetic structure in this species and within other Luculia species.
关键词	Luculia Pinceana Microsatellite Polymorphism
学科领域	Horticulture
收录类别	SCI
语种	英语
WOS记录号	WOS:000277931900021
引用统计
文献类型	期刊论文
条目标识符	http://ir.kib.ac.cn/handle/151853/14109
专题	中国西南野生生物种质资源库
作者单位	1.Chinese Acad Sci, Kunming Inst Bot, Key Lab Biodivers & Biogeog, Kunming 650204, Yunnan, Peoples R China 2.Chinese Acad Sci, Kunming Inst Bot, Germplasm Bank Wild Species, Plant Germplasm & Genom Ctr, Kunming 650204, Yunnan, Peoples R China 3.Chinese Acad Sci, Grad Sch, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Zhou, Wei,Wang, Hong,Li, De-Zhu,et al. Isolation and Characterization of 13 Microsatellite Loci from Luculia pinceana (Rubiaceae), a Typical Distylous Species[J]. HORTSCIENCE,2010,45(5):840-841.
APA	Zhou, Wei,Wang, Hong,Li, De-Zhu,Yang, Jun-Bo,&Zhou, Wei.(2010).Isolation and Characterization of 13 Microsatellite Loci from Luculia pinceana (Rubiaceae), a Typical Distylous Species.HORTSCIENCE,45(5),840-841.
MLA	Zhou, Wei,et al."Isolation and Characterization of 13 Microsatellite Loci from Luculia pinceana (Rubiaceae), a Typical Distylous Species".HORTSCIENCE 45.5(2010):840-841.

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