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题名: Isolation and Characterization of 13 Microsatellite Loci from Luculia pinceana (Rubiaceae), a Typical Distylous Species
作者: Zhou, Wei1; null(王红)1; null(李德铢)1, 2; Yang, Jun-Bo2; Zhou, Wei3
刊名: HORTSCIENCE
关键词: Luculia pinceana ; microsatellite ; polymorphism
英文摘要: Luculia pinceana Hook. (Rubiaceae) is a typical distylous species with dimorphic and long-styled monomorphic populations. Within this study, we developed 13 microsatellite markers from L. pinceana using a modified biotin streptavidin capture method. Polymorphism of each locus was assessed in 30 individuals from four dimorphic populations and one monomorphic population. The average allele number of these microsatellites was 4.153 per locus ranging from three to seven. The observed and expected heterozygosities were from 0.040 to 0.840 and from 0.571 to 0.769, respectively. Additionally, all 13 identified microsatellite markers were successfully amplified in its related species, L. yunnanensis, 10 of which showed polymorphism. These microsatellite markers could provide a useful tool for further study of the breeding system and the population genetic structure in this species and within other Luculia species.
出版日期: 2010-05-01
卷号: 45, 期号:5, 页码:840-841
语种: 英语
收录类别: SCI
学科分类: Horticulture
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.kib.ac.cn/handle/151853/14109
Appears in Collections:中国西南野生生物种质资源库_期刊论文

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作者单位: 1.Chinese Acad Sci, Kunming Inst Bot, Key Lab Biodivers & Biogeog, Kunming 650204, Yunnan, Peoples R China
2.Chinese Acad Sci, Kunming Inst Bot, Germplasm Bank Wild Species, Plant Germplasm & Genom Ctr, Kunming 650204, Yunnan, Peoples R China
3.Chinese Acad Sci, Grad Sch, Beijing 100049, Peoples R China

Recommended Citation:
Zhou, W; Wang, H; Li, DZ; Yang, JB; Zhou, W.Isolation and Characterization of 13 Microsatellite Loci from Luculia pinceana (Rubiaceae), a Typical Distylous Species,HORTSCIENCE,2010,45(5):840-841
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