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Isolation and Characterization of 13 Microsatellite Loci from Luculia pinceana (Rubiaceae), a Typical Distylous Species
Zhou, Wei1; Wang, Hong1; Li, De-Zhu1,2; Yang, Jun-Bo2; Zhou, Wei3
2010-05-01
发表期刊HORTSCIENCE
卷号45期号:5页码:840-841
摘要Luculia pinceana Hook. (Rubiaceae) is a typical distylous species with dimorphic and long-styled monomorphic populations. Within this study, we developed 13 microsatellite markers from L. pinceana using a modified biotin streptavidin capture method. Polymorphism of each locus was assessed in 30 individuals from four dimorphic populations and one monomorphic population. The average allele number of these microsatellites was 4.153 per locus ranging from three to seven. The observed and expected heterozygosities were from 0.040 to 0.840 and from 0.571 to 0.769, respectively. Additionally, all 13 identified microsatellite markers were successfully amplified in its related species, L. yunnanensis, 10 of which showed polymorphism. These microsatellite markers could provide a useful tool for further study of the breeding system and the population genetic structure in this species and within other Luculia species.
关键词Luculia Pinceana Microsatellite Polymorphism
资助信息National Basic Research Program of China (973 Program) [2007CB411600]; Chinese Academy of Sciences [2009-LSF-GBOWS-01]
学科领域Horticulture
收录类别SCI
语种英语
WOS研究方向Agriculture
WOS类目Horticulture
WOS记录号WOS:000277931900021
引用统计
被引频次:4[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.kib.ac.cn/handle/151853/14109
专题中国西南野生生物种质资源库
作者单位1.Chinese Acad Sci, Kunming Inst Bot, Key Lab Biodivers & Biogeog, Kunming 650204, Yunnan, Peoples R China
2.Chinese Acad Sci, Kunming Inst Bot, Germplasm Bank Wild Species, Plant Germplasm & Genom Ctr, Kunming 650204, Yunnan, Peoples R China
3.Chinese Acad Sci, Grad Sch, Beijing 100049, Peoples R China
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Zhou, Wei,Wang, Hong,Li, De-Zhu,et al. Isolation and Characterization of 13 Microsatellite Loci from Luculia pinceana (Rubiaceae), a Typical Distylous Species[J]. HORTSCIENCE,2010,45(5):840-841.
APA Zhou, Wei,Wang, Hong,Li, De-Zhu,Yang, Jun-Bo,&Zhou, Wei.(2010).Isolation and Characterization of 13 Microsatellite Loci from Luculia pinceana (Rubiaceae), a Typical Distylous Species.HORTSCIENCE,45(5),840-841.
MLA Zhou, Wei,et al."Isolation and Characterization of 13 Microsatellite Loci from Luculia pinceana (Rubiaceae), a Typical Distylous Species".HORTSCIENCE 45.5(2010):840-841.
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