Isolation and Characterization of Microsatellite Markers in the Endangered Species Taxus wallichiana Using the FIASCO Method

	Isolation and Characterization of Microsatellite Markers in the Endangered Species Taxus wallichiana Using the FIASCO Method
	Yang, Jun-Bo 1; Li, Hong-Tao1 ; Li, De-Zhu1,2 ; Liu, Jie1,3 ; Gao, Lian-Ming1,2
通讯作者	gaolm@mail.kib.ac.cn
	2009-12-01
发表期刊	HORTSCIENCE
ISSN	0018-5345
卷号	44 期号:7 页码:2043-2045
摘要	The Himalayan yew, Taxus wallichiana Zucc., is an endangered species with a scatted distribution in the Eastern Himalayas and southwestern China. In the present study, 10 microsatellite markers from the genome of T. wallichiana were developed using the protocol of fast isolation by amplified fragment length polymorphism of sequences containing repeats (FIASCO). Polymorphism of each locus was assessed in 28 samples from four wild populations of the Himalayan yew. The allele number of the microsatellites ranged from two to five with an average of 2.9 per allele. The observed and expected heterozygosity varied from 0.00 to 1.00 and from 0.3818 to 0.7552, respectively. Cross-species amplification in another two yew species showed eight of them holding promise for sister species. Two of the 10 loci (TG126 and TC49) significantly deviated from Hardy-Weinberg expectations. No significant linkage disequilibrium was detected between the comparisons of these loci. These polymorphic microsatellite markers would be useful tools for population genetics studies and assessing genetic variations to establish conservation strategy of this endangered species.
关键词	Himalayan Yew Population Genetics Linkage Disequilibrium
学科领域	Horticulture
收录类别	SCI
语种	英语
WOS记录号	WOS:000272751600047
引用统计	被引频次：15[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.kib.ac.cn/handle/151853/14037
专题	中国科学院东亚植物多样性与生物地理学重点实验室
作者单位	1.Chinese Acad Sci, Germplasm Bank Wild Species, Kunming Inst Bot, Kunming 650204, Yunnan, Peoples R China 2.Chinese Acad Sci, Key Lab Biodivers & Biogeog, Kunming Inst Bot, Kunming 650204, Yunnan, Peoples R China 3.Chinese Acad Sci, Grad Sch, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Yang, Jun-Bo,Li, Hong-Tao,Li, De-Zhu,et al. Isolation and Characterization of Microsatellite Markers in the Endangered Species Taxus wallichiana Using the FIASCO Method[J]. HORTSCIENCE,2009,44(7):2043-2045.
APA	Yang, Jun-Bo,Li, Hong-Tao,Li, De-Zhu,Liu, Jie,&Gao, Lian-Ming.(2009).Isolation and Characterization of Microsatellite Markers in the Endangered Species Taxus wallichiana Using the FIASCO Method.HORTSCIENCE,44(7),2043-2045.
MLA	Yang, Jun-Bo,et al."Isolation and Characterization of Microsatellite Markers in the Endangered Species Taxus wallichiana Using the FIASCO Method".HORTSCIENCE 44.7(2009):2043-2045.

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