Isolation and Characterization of Microsatellite Markers in the Endangered Species Taxus wallichiana Using the FIASCO Method
Yang, Jun-Bo1; Li, Hong-Tao1; Li, De-Zhu1,2; Liu, Jie1,3; Gao, Lian-Ming1,2
2009-12-01
发表期刊HORTSCIENCE
ISSN0018-5345
卷号44期号:7页码:2043-2045
摘要The Himalayan yew, Taxus wallichiana Zucc., is an endangered species with a scatted distribution in the Eastern Himalayas and southwestern China. In the present study, 10 microsatellite markers from the genome of T. wallichiana were developed using the protocol of fast isolation by amplified fragment length polymorphism of sequences containing repeats (FIASCO). Polymorphism of each locus was assessed in 28 samples from four wild populations of the Himalayan yew. The allele number of the microsatellites ranged from two to five with an average of 2.9 per allele. The observed and expected heterozygosity varied from 0.00 to 1.00 and from 0.3818 to 0.7552, respectively. Cross-species amplification in another two yew species showed eight of them holding promise for sister species. Two of the 10 loci (TG126 and TC49) significantly deviated from Hardy-Weinberg expectations. No significant linkage disequilibrium was detected between the comparisons of these loci. These polymorphic microsatellite markers would be useful tools for population genetics studies and assessing genetic variations to establish conservation strategy of this endangered species.
关键词Himalayan Yew Population Genetics Linkage Disequilibrium
资助信息National Natural Sciences Foundation of China [30700042]; Natural Sciences Foundation of Yunnan [2007C088M]; Kunming Institute of Botany of CAS [540806321211]; GBOWS ; West Light Foundation of The Chinese Academy of Sciences [9223111W1]; Yunnan Province of China [2008PY064]
学科领域Horticulture
收录类别SCI
语种英语
WOS研究方向Agriculture
WOS类目Horticulture
WOS记录号WOS:000272751600047
引用统计
被引频次:11[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.kib.ac.cn/handle/151853/14037
专题中国科学院东亚植物多样性与生物地理学重点实验室
作者单位1.Chinese Acad Sci, Germplasm Bank Wild Species, Kunming Inst Bot, Kunming 650204, Yunnan, Peoples R China
2.Chinese Acad Sci, Key Lab Biodivers & Biogeog, Kunming Inst Bot, Kunming 650204, Yunnan, Peoples R China
3.Chinese Acad Sci, Grad Sch, Beijing 100049, Peoples R China
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Yang, Jun-Bo,Li, Hong-Tao,Li, De-Zhu,et al. Isolation and Characterization of Microsatellite Markers in the Endangered Species Taxus wallichiana Using the FIASCO Method[J]. HORTSCIENCE,2009,44(7):2043-2045.
APA Yang, Jun-Bo,Li, Hong-Tao,Li, De-Zhu,Liu, Jie,&Gao, Lian-Ming.(2009).Isolation and Characterization of Microsatellite Markers in the Endangered Species Taxus wallichiana Using the FIASCO Method.HORTSCIENCE,44(7),2043-2045.
MLA Yang, Jun-Bo,et al."Isolation and Characterization of Microsatellite Markers in the Endangered Species Taxus wallichiana Using the FIASCO Method".HORTSCIENCE 44.7(2009):2043-2045.
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