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题名: Isolation and Characterization of Microsatellite Markers in the Endangered Species Taxus wallichiana Using the FIASCO Method
作者: Yang, Jun-Bo1; Li, Hong-Tao1; null(李德铢)1, 2; Liu, Jie1, 3; null(高连明)1, 2
刊名: HORTSCIENCE
关键词: Himalayan yew ; population genetics ; linkage disequilibrium
英文摘要: The Himalayan yew, Taxus wallichiana Zucc., is an endangered species with a scatted distribution in the Eastern Himalayas and southwestern China. In the present study, 10 microsatellite markers from the genome of T. wallichiana were developed using the protocol of fast isolation by amplified fragment length polymorphism of sequences containing repeats (FIASCO). Polymorphism of each locus was assessed in 28 samples from four wild populations of the Himalayan yew. The allele number of the microsatellites ranged from two to five with an average of 2.9 per allele. The observed and expected heterozygosity varied from 0.00 to 1.00 and from 0.3818 to 0.7552, respectively. Cross-species amplification in another two yew species showed eight of them holding promise for sister species. Two of the 10 loci (TG126 and TC49) significantly deviated from Hardy-Weinberg expectations. No significant linkage disequilibrium was detected between the comparisons of these loci. These polymorphic microsatellite markers would be useful tools for population genetics studies and assessing genetic variations to establish conservation strategy of this endangered species.
出版日期: 2009-12-01
卷号: 44, 期号:7, 页码:2043-2045
语种: 英语
收录类别: SCI
学科分类: Horticulture
ISSN号: 0018-5345
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.kib.ac.cn/handle/151853/14037
Appears in Collections:中国科学院东亚植物多样性与生物地理学重点实验室_期刊论文

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作者单位: 1.Chinese Acad Sci, Germplasm Bank Wild Species, Kunming Inst Bot, Kunming 650204, Yunnan, Peoples R China
2.Chinese Acad Sci, Key Lab Biodivers & Biogeog, Kunming Inst Bot, Kunming 650204, Yunnan, Peoples R China
3.Chinese Acad Sci, Grad Sch, Beijing 100049, Peoples R China

Recommended Citation:
Yang, JB; Li, HT; Li, DZ; Liu, J; Gao, LM.Isolation and Characterization of Microsatellite Markers in the Endangered Species Taxus wallichiana Using the FIASCO Method,HORTSCIENCE,2009,44(7):2043-2045
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