Application of Real-Time Scorpion PCR for Authentication and Quantification of the Traditional Chinese Medicinal Plant Drynaria fortunei
Xue, Chun-Ying2; Xue, Heng-Gang1
2008-09-01
发表期刊PLANTA MEDICA
ISSN0032-0943
卷号74期号:11页码:1416-1420
摘要DNA sequence analysis of the trnL-trnF spacer region and real-time scorpion polymerase chain reaction were exploited for their applications in the differentiation and quantification of the traditional Chinese medicinal plants Drynaria fortunei from five related adulterants: D. mollis, D. quercifolia, D. rigidula, D. sparsisora, and Pseudodrynaria coronans. The data demonstrated that variations in the trnL-trnF spacer regions were very low at the intraspecific level but extremely high at the interspecific level, meaning that they could be easily distinguished at the DNA level. The sequence difference allowed effective and reliable differentiation of D. fortunei from adulterants by real-time scorpion PCR. Furthermore, a quantification methodology was carried out to quantify D. fortunei.
关键词Traditional Chinese Medicinal Plants Drynaria Fortunei Polypodiaceae Real-time Scorpion Polymerase Chain Reaction
资助信息Natural Science Foundation of China (NSFC) [30770153]; Natural Science Foundation of Yunnan (NSFN) [2006C 0050 M]
学科领域Plant Sciences ; Chemistry, Medicinal ; Pharmacology & Pharmacy
DOI10.1055/s-2008-1081305
收录类别SCI
语种英语
WOS研究方向Plant Sciences ; Pharmacology & Pharmacy
WOS类目Plant Sciences ; Chemistry, Medicinal ; Pharmacology & Pharmacy
WOS记录号WOS:000260174300018
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被引频次:6[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.kib.ac.cn/handle/151853/13955
专题中国科学院东亚植物多样性与生物地理学重点实验室
作者单位1.Peoples Liberat Army Gen Hosp, Beijing 100853, Peoples R China
2.Chinese Acad Sci, Kunming Inst Bot, Key Lab Biodivers & Biogeog, Kunming, Yunnan, Peoples R China
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Xue, Chun-Ying,Xue, Heng-Gang. Application of Real-Time Scorpion PCR for Authentication and Quantification of the Traditional Chinese Medicinal Plant Drynaria fortunei[J]. PLANTA MEDICA,2008,74(11):1416-1420.
APA Xue, Chun-Ying,&Xue, Heng-Gang.(2008).Application of Real-Time Scorpion PCR for Authentication and Quantification of the Traditional Chinese Medicinal Plant Drynaria fortunei.PLANTA MEDICA,74(11),1416-1420.
MLA Xue, Chun-Ying,et al."Application of Real-Time Scorpion PCR for Authentication and Quantification of the Traditional Chinese Medicinal Plant Drynaria fortunei".PLANTA MEDICA 74.11(2008):1416-1420.
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