Application of Real-Time Scorpion PCR for Authentication and Quantification of the Traditional Chinese Medicinal Plant Drynaria fortunei
Xue, Chun-Ying2; Xue, Heng-Gang1
Corresponding Authorxuegangdyx@yahoo.com.cn
2008-09-01
Source PublicationPLANTA MEDICA
ISSN0032-0943
Volume74Issue:11Pages:1416-1420
AbstractDNA sequence analysis of the trnL-trnF spacer region and real-time scorpion polymerase chain reaction were exploited for their applications in the differentiation and quantification of the traditional Chinese medicinal plants Drynaria fortunei from five related adulterants: D. mollis, D. quercifolia, D. rigidula, D. sparsisora, and Pseudodrynaria coronans. The data demonstrated that variations in the trnL-trnF spacer regions were very low at the intraspecific level but extremely high at the interspecific level, meaning that they could be easily distinguished at the DNA level. The sequence difference allowed effective and reliable differentiation of D. fortunei from adulterants by real-time scorpion PCR. Furthermore, a quantification methodology was carried out to quantify D. fortunei.
KeywordTraditional Chinese Medicinal Plants Drynaria Fortunei Polypodiaceae Real-time Scorpion Polymerase Chain Reaction
Subject AreaPlant Sciences ; Chemistry, Medicinal ; Pharmacology & Pharmacy
DOI10.1055/s-2008-1081305
Indexed BySCI
Language英语
WOS Research AreaPlant Sciences ; Pharmacology & Pharmacy
WOS SubjectPlant Sciences ; Chemistry, Medicinal ; Pharmacology & Pharmacy
WOS IDWOS:000260174300018
Citation statistics
Cited Times:6[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.kib.ac.cn/handle/151853/13955
Collection中国科学院东亚植物多样性与生物地理学重点实验室
Affiliation1.Peoples Liberat Army Gen Hosp, Beijing 100853, Peoples R China
2.Chinese Acad Sci, Kunming Inst Bot, Key Lab Biodivers & Biogeog, Kunming, Yunnan, Peoples R China
Recommended Citation
GB/T 7714
Xue, Chun-Ying,Xue, Heng-Gang. Application of Real-Time Scorpion PCR for Authentication and Quantification of the Traditional Chinese Medicinal Plant Drynaria fortunei[J]. PLANTA MEDICA,2008,74(11):1416-1420.
APA Xue, Chun-Ying,&Xue, Heng-Gang.(2008).Application of Real-Time Scorpion PCR for Authentication and Quantification of the Traditional Chinese Medicinal Plant Drynaria fortunei.PLANTA MEDICA,74(11),1416-1420.
MLA Xue, Chun-Ying,et al."Application of Real-Time Scorpion PCR for Authentication and Quantification of the Traditional Chinese Medicinal Plant Drynaria fortunei".PLANTA MEDICA 74.11(2008):1416-1420.
Files in This Item:
File Name/Size DocType Version Access License
PLANTA MEDICA74 1416(4445KB) 开放获取--View Download
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Xue, Chun-Ying]'s Articles
[Xue, Heng-Gang]'s Articles
Baidu academic
Similar articles in Baidu academic
[Xue, Chun-Ying]'s Articles
[Xue, Heng-Gang]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Xue, Chun-Ying]'s Articles
[Xue, Heng-Gang]'s Articles
Terms of Use
No data!
Social Bookmark/Share
File name: PLANTA MEDICA74 1416-1420.pdf
Format: Adobe PDF
This file does not support browsing at this time
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.