Application of Real-Time Scorpion PCR for Authentication and Quantification of the Traditional Chinese Medicinal Plant Drynaria fortunei

doi:10.1055/s-2008-1081305

	Application of Real-Time Scorpion PCR for Authentication and Quantification of the Traditional Chinese Medicinal Plant Drynaria fortunei
	Xue, Chun-Ying2 ; Xue, Heng-Gang 1
通讯作者	xuegangdyx@yahoo.com.cn
	2008-09-01
发表期刊	PLANTA MEDICA
ISSN	0032-0943
卷号	74 期号:11 页码:1416-1420
摘要	DNA sequence analysis of the trnL-trnF spacer region and real-time scorpion polymerase chain reaction were exploited for their applications in the differentiation and quantification of the traditional Chinese medicinal plants Drynaria fortunei from five related adulterants: D. mollis, D. quercifolia, D. rigidula, D. sparsisora, and Pseudodrynaria coronans. The data demonstrated that variations in the trnL-trnF spacer regions were very low at the intraspecific level but extremely high at the interspecific level, meaning that they could be easily distinguished at the DNA level. The sequence difference allowed effective and reliable differentiation of D. fortunei from adulterants by real-time scorpion PCR. Furthermore, a quantification methodology was carried out to quantify D. fortunei.
关键词	Traditional Chinese Medicinal Plants Drynaria Fortunei Polypodiaceae Real-time Scorpion Polymerase Chain Reaction
学科领域	Plant Sciences ; Chemistry, Medicinal ; Pharmacology & Pharmacy
DOI	10.1055/s-2008-1081305
收录类别	SCI
语种	英语
WOS记录号	WOS:000260174300018
引用统计	被引频次：8[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.kib.ac.cn/handle/151853/13955
专题	中国科学院东亚植物多样性与生物地理学重点实验室
作者单位	1.Peoples Liberat Army Gen Hosp, Beijing 100853, Peoples R China 2.Chinese Acad Sci, Kunming Inst Bot, Key Lab Biodivers & Biogeog, Kunming, Yunnan, Peoples R China
推荐引用方式 GB/T 7714	Xue, Chun-Ying,Xue, Heng-Gang. Application of Real-Time Scorpion PCR for Authentication and Quantification of the Traditional Chinese Medicinal Plant Drynaria fortunei[J]. PLANTA MEDICA,2008,74(11):1416-1420.
APA	Xue, Chun-Ying,&Xue, Heng-Gang.(2008).Application of Real-Time Scorpion PCR for Authentication and Quantification of the Traditional Chinese Medicinal Plant Drynaria fortunei.PLANTA MEDICA,74(11),1416-1420.
MLA	Xue, Chun-Ying,et al."Application of Real-Time Scorpion PCR for Authentication and Quantification of the Traditional Chinese Medicinal Plant Drynaria fortunei".PLANTA MEDICA 74.11(2008):1416-1420.

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