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题名: Application of Real-Time Scorpion PCR for Authentication and Quantification of the Traditional Chinese Medicinal Plant Drynaria fortunei
作者: Xue, Chun-Ying2; Xue, Heng-Gang1
刊名: PLANTA MEDICA
关键词: traditional Chinese medicinal plants ; Drynaria fortunei ; Polypodiaceae ; real-time scorpion polymerase chain reaction
英文摘要: DNA sequence analysis of the trnL-trnF spacer region and real-time scorpion polymerase chain reaction were exploited for their applications in the differentiation and quantification of the traditional Chinese medicinal plants Drynaria fortunei from five related adulterants: D. mollis, D. quercifolia, D. rigidula, D. sparsisora, and Pseudodrynaria coronans. The data demonstrated that variations in the trnL-trnF spacer regions were very low at the intraspecific level but extremely high at the interspecific level, meaning that they could be easily distinguished at the DNA level. The sequence difference allowed effective and reliable differentiation of D. fortunei from adulterants by real-time scorpion PCR. Furthermore, a quantification methodology was carried out to quantify D. fortunei.
出版日期: 2008-09-01
卷号: 74, 期号:11, 页码:1416-1420
语种: 英语
收录类别: SCI
学科分类: Plant Sciences; Chemistry, Medicinal; Pharmacology & Pharmacy
ISSN号: 0032-0943
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.kib.ac.cn/handle/151853/13955
Appears in Collections:中国科学院东亚植物多样性与生物地理学重点实验室_期刊论文

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作者单位: 1.Peoples Liberat Army Gen Hosp, Beijing 100853, Peoples R China
2.Chinese Acad Sci, Kunming Inst Bot, Key Lab Biodivers & Biogeog, Kunming, Yunnan, Peoples R China

Recommended Citation:
Xue, CY; Xue, HG.Application of Real-Time Scorpion PCR for Authentication and Quantification of the Traditional Chinese Medicinal Plant Drynaria fortunei,PLANTA MEDICA,2008,74(11):1416-1420
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