| Differentiation of the traditional Chinese medicinal plants Euphorbia humifusa and E. maculata from adulterants by TaqMan real-time polymerase chain reaction | |
Xue, Heng-Gang2; Wang, Hong1 ; Li, De-Zhu1; Xue, Chun-Ying1; Wang, Qing-Zhong3
| |
| 通讯作者 | chyxue@yahoo.cn |
| 2008-02-01 | |
| 发表期刊 | PLANTA MEDICA
 |
| ISSN | 0032-0943 |
| 卷号 | 74期号:3页码:302-304 |
| 摘要 | DNA sequence analysis of the rDNA internal transcribed spacer 1 (ITS1) and TaqMan real-time polymerase chain reactionwere exploited for their applications in the differentiation of the traditional Chinese medicinal plants Euphorbia humifusa and E. maculata from three related adulterants E. hypericifolia, E. atoto and E. prostrata. The data demonstrated that variations in the ITS1 regions were very low at the intra-species level but extremely high at the inter-species level, so that they could be easily distinguished at the DNA level. The sequence difference allowed an effective and reliable differentiation of E. hurnifusa and E. maculata from the adulterants by TaqMan real-time PCR. |
| 关键词 | Traditional Chinese Medicinal Plants Euphorbia Humifusa Euphorbia Maculata Euphorbiaceae Taqman Real-time Polymerase Chain Reaction |
| 学科领域 | Plant Sciences ; Medicinal ; Chemistry ; Pharmacology & Pharmacy |
| DOI | 10.1055/s-2008-1034305 |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000254630300019 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.kib.ac.cn/handle/151853/13953 |
| 专题 | 中国科学院东亚植物多样性与生物地理学重点实验室 |
| 作者单位 | 1.Chinese Acad Sci, Kunming Inst Bot, Key Lab Biodivers & Biog, Kunming 650204, Yunnan, Peoples R China 2.Peoples Liberat Army Gen Hosp, Beijing 100853, Peoples R China 3.Yunnan Univ, Sch Chem Sci & Technol, Kunming 650091, Peoples R China |
| 推荐引用方式 GB/T 7714 | Xue, Heng-Gang,Wang, Hong,Li, De-Zhu,et al. Differentiation of the traditional Chinese medicinal plants Euphorbia humifusa and E. maculata from adulterants by TaqMan real-time polymerase chain reaction[J]. PLANTA MEDICA,2008,74(3):302-304. |
| APA | Xue, Heng-Gang,Wang, Hong,Li, De-Zhu,Xue, Chun-Ying,&Wang, Qing-Zhong.(2008).Differentiation of the traditional Chinese medicinal plants Euphorbia humifusa and E. maculata from adulterants by TaqMan real-time polymerase chain reaction.PLANTA MEDICA,74(3),302-304. |
| MLA | Xue, Heng-Gang,et al."Differentiation of the traditional Chinese medicinal plants Euphorbia humifusa and E. maculata from adulterants by TaqMan real-time polymerase chain reaction".PLANTA MEDICA 74.3(2008):302-304. |
| 条目包含的文件 | 下载所有文件 | |||||
| 文件名称/大小 | 文献类型 | 版本类型 | 开放类型 | 使用许可 | ||
| Differentiation of t（153KB） | 开放获取 | CC BY-NC-SA | 浏览 下载 | |||
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。
修改评论