Differentiation of the traditional Chinese medicinal plants Euphorbia humifusa and E. maculata from adulterants by TaqMan real-time polymerase chain reaction
Xue, Heng-Gang2; Wang, Hong1; Li, De-Zhu1; Xue, Chun-Ying1; Wang, Qing-Zhong3
2008-02-01
发表期刊PLANTA MEDICA
ISSN0032-0943
卷号74期号:3页码:302-304
摘要DNA sequence analysis of the rDNA internal transcribed spacer 1 (ITS1) and TaqMan real-time polymerase chain reactionwere exploited for their applications in the differentiation of the traditional Chinese medicinal plants Euphorbia humifusa and E. maculata from three related adulterants E. hypericifolia, E. atoto and E. prostrata. The data demonstrated that variations in the ITS1 regions were very low at the intra-species level but extremely high at the inter-species level, so that they could be easily distinguished at the DNA level. The sequence difference allowed an effective and reliable differentiation of E. hurnifusa and E. maculata from the adulterants by TaqMan real-time PCR.
关键词Traditional Chinese Medicinal Plants Euphorbia Humifusa Euphorbia Maculata Euphorbiaceae Taqman Real-time Polymerase Chain Reaction
学科领域Plant Sciences ; Medicinal ; Chemistry ; Pharmacology & Pharmacy
DOI10.1055/s-2008-1034305
收录类别SCI
语种英语
WOS研究方向Plant Sciences ; Pharmacology & Pharmacy
WOS类目Plant Sciences ; Chemistry, Medicinal ; Pharmacology & Pharmacy
WOS记录号WOS:000254630300019
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被引频次:4[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.kib.ac.cn/handle/151853/13953
专题中国科学院东亚植物多样性与生物地理学重点实验室
作者单位1.Chinese Acad Sci, Kunming Inst Bot, Key Lab Biodivers & Biog, Kunming 650204, Yunnan, Peoples R China
2.Peoples Liberat Army Gen Hosp, Beijing 100853, Peoples R China
3.Yunnan Univ, Sch Chem Sci & Technol, Kunming 650091, Peoples R China
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Xue, Heng-Gang,Wang, Hong,Li, De-Zhu,et al. Differentiation of the traditional Chinese medicinal plants Euphorbia humifusa and E. maculata from adulterants by TaqMan real-time polymerase chain reaction[J]. PLANTA MEDICA,2008,74(3):302-304.
APA Xue, Heng-Gang,Wang, Hong,Li, De-Zhu,Xue, Chun-Ying,&Wang, Qing-Zhong.(2008).Differentiation of the traditional Chinese medicinal plants Euphorbia humifusa and E. maculata from adulterants by TaqMan real-time polymerase chain reaction.PLANTA MEDICA,74(3),302-304.
MLA Xue, Heng-Gang,et al."Differentiation of the traditional Chinese medicinal plants Euphorbia humifusa and E. maculata from adulterants by TaqMan real-time polymerase chain reaction".PLANTA MEDICA 74.3(2008):302-304.
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