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题名: Differentiation of the traditional Chinese medicinal plants Euphorbia humifusa and E. maculata from adulterants by TaqMan real-time polymerase chain reaction
作者: Xue, Heng-Gang2; null(王红)1; null(李德铢)1; Xue, Chun-Ying1; Wang, Qing-Zhong3
刊名: PLANTA MEDICA
关键词: traditional chinese medicinal plants ; euphorbia humifusa ; euphorbia maculata ; euphorbiaceae TaqMan real-time polymerase chain reaction
英文摘要: DNA sequence analysis of the rDNA internal transcribed spacer 1 (ITS1) and TaqMan real-time polymerase chain reactionwere exploited for their applications in the differentiation of the traditional Chinese medicinal plants Euphorbia humifusa and E. maculata from three related adulterants E. hypericifolia, E. atoto and E. prostrata. The data demonstrated that variations in the ITS1 regions were very low at the intra-species level but extremely high at the inter-species level, so that they could be easily distinguished at the DNA level. The sequence difference allowed an effective and reliable differentiation of E. hurnifusa and E. maculata from the adulterants by TaqMan real-time PCR.
出版日期: 2008-02-01
卷号: 74, 期号:3, 页码:302-304
DOI标识: 10.1055/s-2008-1034305
语种: 英语
收录类别: SCI
学科分类: Plant Sciences; Chemistry ; Medicinal; Pharmacology & Pharmacy
ISSN号: 0032-0943
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.kib.ac.cn/handle/151853/13953
Appears in Collections:中国科学院东亚植物多样性与生物地理学重点实验室_期刊论文

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作者单位: 1.Chinese Acad Sci, Kunming Inst Bot, Key Lab Biodivers & Biog, Kunming 650204, Yunnan, Peoples R China
2.Peoples Liberat Army Gen Hosp, Beijing 100853, Peoples R China
3.Yunnan Univ, Sch Chem Sci & Technol, Kunming 650091, Peoples R China
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