Differentiation of the traditional Chinese medicinal plants Euphorbia humifusa and E. maculata from adulterants by TaqMan real-time polymerase chain reaction
Xue, Heng-Gang2; Wang, Hong1; Li, De-Zhu1; Xue, Chun-Ying1; Wang, Qing-Zhong3
Corresponding Authorchyxue@yahoo.cn
2008-02-01
Source PublicationPLANTA MEDICA
ISSN0032-0943
Volume74Issue:3Pages:302-304
AbstractDNA sequence analysis of the rDNA internal transcribed spacer 1 (ITS1) and TaqMan real-time polymerase chain reactionwere exploited for their applications in the differentiation of the traditional Chinese medicinal plants Euphorbia humifusa and E. maculata from three related adulterants E. hypericifolia, E. atoto and E. prostrata. The data demonstrated that variations in the ITS1 regions were very low at the intra-species level but extremely high at the inter-species level, so that they could be easily distinguished at the DNA level. The sequence difference allowed an effective and reliable differentiation of E. hurnifusa and E. maculata from the adulterants by TaqMan real-time PCR.
KeywordTraditional Chinese Medicinal Plants Euphorbia Humifusa Euphorbia Maculata Euphorbiaceae Taqman Real-time Polymerase Chain Reaction
Subject AreaPlant Sciences ; Medicinal ; Chemistry ; Pharmacology & Pharmacy
DOI10.1055/s-2008-1034305
Indexed BySCI
Language英语
WOS Research AreaPlant Sciences ; Pharmacology & Pharmacy
WOS SubjectPlant Sciences ; Chemistry, Medicinal ; Pharmacology & Pharmacy
WOS IDWOS:000254630300019
Citation statistics
Cited Times:4[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.kib.ac.cn/handle/151853/13953
Collection中国科学院东亚植物多样性与生物地理学重点实验室
Affiliation1.Chinese Acad Sci, Kunming Inst Bot, Key Lab Biodivers & Biog, Kunming 650204, Yunnan, Peoples R China
2.Peoples Liberat Army Gen Hosp, Beijing 100853, Peoples R China
3.Yunnan Univ, Sch Chem Sci & Technol, Kunming 650091, Peoples R China
Recommended Citation
GB/T 7714
Xue, Heng-Gang,Wang, Hong,Li, De-Zhu,et al. Differentiation of the traditional Chinese medicinal plants Euphorbia humifusa and E. maculata from adulterants by TaqMan real-time polymerase chain reaction[J]. PLANTA MEDICA,2008,74(3):302-304.
APA Xue, Heng-Gang,Wang, Hong,Li, De-Zhu,Xue, Chun-Ying,&Wang, Qing-Zhong.(2008).Differentiation of the traditional Chinese medicinal plants Euphorbia humifusa and E. maculata from adulterants by TaqMan real-time polymerase chain reaction.PLANTA MEDICA,74(3),302-304.
MLA Xue, Heng-Gang,et al."Differentiation of the traditional Chinese medicinal plants Euphorbia humifusa and E. maculata from adulterants by TaqMan real-time polymerase chain reaction".PLANTA MEDICA 74.3(2008):302-304.
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