Application of LightCycler Polymerase Chain Reaction and Melting Curve Analysis to the Authentication of the Traditional Chinese Medicinal Plant Cimicifuga foetida

doi:10.1055/s-0029-1185403

	Application of LightCycler Polymerase Chain Reaction and Melting Curve Analysis to the Authentication of the Traditional Chinese Medicinal Plant Cimicifuga foetida
	Xue, Chun-Ying1 ; Li, De-Zhu1 ; Wang, Qing-Zhong 2
通讯作者	chyxue@yahoo.cn
	2009-06-01
发表期刊	PLANTA MEDICA
ISSN	0032-0943
卷号	75 期号:8 页码:873-875
摘要	DNA sequence analysis of rDNA internal transcribed spacer (ITS) and fluorescence melting curve analysis of LightCycler real-time polymerase chain reaction products were exploited for their applications in the authentication of the traditional Chinese medicinal plant Cimicifuga foetida from four substitutes: C. heracleifolia, C. dahurica, C. acerina, and C. simplex. According to the melting temperature - which is a function of the GC/AT ratio, length, and nucleotide sequences of the amplified product - C. foetida was differentiated from C. heracleifolia, C. dahurica, C. acerina, and C. simplex. Melting curve analysis offers a rapid and reliable method for the authentication of the traditional Chinese medicinal plant C. foetida.
关键词	Traditional Chinese Medicinal Plant Cimicifuga Foetida Ranunculaceae Lightcycler Real-time Polymerase Chain Reaction Fluorescence Melting Curve Analysis
学科领域	Plant Sciences ; Chemistry, Medicinal ; Pharmacology & Pharmacy
DOI	10.1055/s-0029-1185403
收录类别	SCI
语种	英语
WOS记录号	WOS:000267668700017
引用统计
文献类型	期刊论文
条目标识符	http://ir.kib.ac.cn/handle/151853/13951
专题	中国科学院东亚植物多样性与生物地理学重点实验室
作者单位	1.Chinese Acad Sci, Kunming Inst Bot, Key Lab Biodivers & Biogeog, Kunming 650204, Yunnan, Peoples R China 2.Yunnan Univ, Dept Chem, Kunming 650091, Yunnan, Peoples R China
推荐引用方式 GB/T 7714	Xue, Chun-Ying,Li, De-Zhu,Wang, Qing-Zhong. Application of LightCycler Polymerase Chain Reaction and Melting Curve Analysis to the Authentication of the Traditional Chinese Medicinal Plant Cimicifuga foetida[J]. PLANTA MEDICA,2009,75(8):873-875.
APA	Xue, Chun-Ying,Li, De-Zhu,&Wang, Qing-Zhong.(2009).Application of LightCycler Polymerase Chain Reaction and Melting Curve Analysis to the Authentication of the Traditional Chinese Medicinal Plant Cimicifuga foetida.PLANTA MEDICA,75(8),873-875.
MLA	Xue, Chun-Ying,et al."Application of LightCycler Polymerase Chain Reaction and Melting Curve Analysis to the Authentication of the Traditional Chinese Medicinal Plant Cimicifuga foetida".PLANTA MEDICA 75.8(2009):873-875.

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