Application of LightCycler Polymerase Chain Reaction and Melting Curve Analysis to the Authentication of the Traditional Chinese Medicinal Plant Cimicifuga foetida
Xue, Chun-Ying1; Li, De-Zhu1; Wang, Qing-Zhong2
2009-06-01
发表期刊PLANTA MEDICA
ISSN0032-0943
卷号75期号:8页码:873-875
摘要DNA sequence analysis of rDNA internal transcribed spacer (ITS) and fluorescence melting curve analysis of LightCycler real-time polymerase chain reaction products were exploited for their applications in the authentication of the traditional Chinese medicinal plant Cimicifuga foetida from four substitutes: C. heracleifolia, C. dahurica, C. acerina, and C. simplex. According to the melting temperature - which is a function of the GC/AT ratio, length, and nucleotide sequences of the amplified product - C. foetida was differentiated from C. heracleifolia, C. dahurica, C. acerina, and C. simplex. Melting curve analysis offers a rapid and reliable method for the authentication of the traditional Chinese medicinal plant C. foetida.
关键词Traditional Chinese Medicinal Plant Cimicifuga Foetida Ranunculaceae Lightcycler Real-time Polymerase Chain Reaction Fluorescence Melting Curve Analysis
资助信息Natural Science Foundation of China [NSFC 30770153]
收录类别SCI
语种英语
引用统计
文献类型期刊论文
条目标识符http://ir.kib.ac.cn/handle/151853/13951
专题中国科学院东亚植物多样性与生物地理学重点实验室
作者单位1.Chinese Acad Sci, Kunming Inst Bot, Key Lab Biodivers & Biogeog, Kunming 650204, Yunnan, Peoples R China
2.Yunnan Univ, Dept Chem, Kunming 650091, Yunnan, Peoples R China
推荐引用方式
GB/T 7714
Xue, Chun-Ying,Li, De-Zhu,Wang, Qing-Zhong. Application of LightCycler Polymerase Chain Reaction and Melting Curve Analysis to the Authentication of the Traditional Chinese Medicinal Plant Cimicifuga foetida[J]. PLANTA MEDICA,2009,75(8):873-875.
APA Xue, Chun-Ying,Li, De-Zhu,&Wang, Qing-Zhong.(2009).Application of LightCycler Polymerase Chain Reaction and Melting Curve Analysis to the Authentication of the Traditional Chinese Medicinal Plant Cimicifuga foetida.PLANTA MEDICA,75(8),873-875.
MLA Xue, Chun-Ying,et al."Application of LightCycler Polymerase Chain Reaction and Melting Curve Analysis to the Authentication of the Traditional Chinese Medicinal Plant Cimicifuga foetida".PLANTA MEDICA 75.8(2009):873-875.
条目包含的文件
文件名称/大小 文献类型 版本类型 开放类型 使用许可
PLANTA MEDICA75 873-(3256KB) 开放获取--请求全文
个性服务
推荐该条目
保存到收藏夹
查看访问统计
导出为Endnote文件
谷歌学术
谷歌学术中相似的文章
[Xue, Chun-Ying]的文章
[Li, De-Zhu]的文章
[Wang, Qing-Zhong]的文章
百度学术
百度学术中相似的文章
[Xue, Chun-Ying]的文章
[Li, De-Zhu]的文章
[Wang, Qing-Zhong]的文章
必应学术
必应学术中相似的文章
[Xue, Chun-Ying]的文章
[Li, De-Zhu]的文章
[Wang, Qing-Zhong]的文章
相关权益政策
暂无数据
收藏/分享
所有评论 (0)
暂无评论
 

除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。