中国科学院昆明植物研究所机构知识库
Advanced  
KIB OpenIR  > 中国科学院东亚植物多样性与生物地理学重点实验室  > 期刊论文
题名: Application of LightCycler Polymerase Chain Reaction and Melting Curve Analysis to the Authentication of the Traditional Chinese Medicinal Plant Cimicifuga foetida
作者: Xue, Chun-Ying1; null(李德铢)1; Wang, Qing-Zhong2
刊名: PLANTA MEDICA
关键词: traditional Chinese medicinal plant ; Cimicifuga foetida ; Ranunculaceae ; LightCycler real-time polymerase chain reaction ; fluorescence melting curve analysis
英文摘要: DNA sequence analysis of rDNA internal transcribed spacer (ITS) and fluorescence melting curve analysis of LightCycler real-time polymerase chain reaction products were exploited for their applications in the authentication of the traditional Chinese medicinal plant Cimicifuga foetida from four substitutes: C. heracleifolia, C. dahurica, C. acerina, and C. simplex. According to the melting temperature - which is a function of the GC/AT ratio, length, and nucleotide sequences of the amplified product - C. foetida was differentiated from C. heracleifolia, C. dahurica, C. acerina, and C. simplex. Melting curve analysis offers a rapid and reliable method for the authentication of the traditional Chinese medicinal plant C. foetida.
出版日期: 2009-06-01
卷号: 75, 期号:8, 页码:873-875
DOI标识: 10.1055/s-0029-1185403
语种: 英语
ISSN号: 0032-0943
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.kib.ac.cn/handle/151853/13951
Appears in Collections:中国科学院东亚植物多样性与生物地理学重点实验室_期刊论文

Files in This Item:
File Name/ File Size Content Type Version Access License
PLANTA MEDICA75 873-875.pdf(3256KB)----开放获取--View 联系获取全文

作者单位: 1.Chinese Acad Sci, Kunming Inst Bot, Key Lab Biodivers & Biogeog, Kunming 650204, Yunnan, Peoples R China
2.Yunnan Univ, Dept Chem, Kunming 650091, Yunnan, Peoples R China

Recommended Citation:
Xue, CY; Li, DZ; Wang, QZ.Application of LightCycler Polymerase Chain Reaction and Melting Curve Analysis to the Authentication of the Traditional Chinese Medicinal Plant Cimicifuga foetida,PLANTA MEDICA,2009,75(8):873-875
Service
Recommend this item
Sava as my favorate item
Show this item's statistics
Export Endnote File
Google Scholar
Similar articles in Google Scholar
[Xue, Chun-Ying]'s Articles
[Li, De-Zhu]'s Articles
[Wang, Qing-Zhong]'s Articles
CSDL cross search
Similar articles in CSDL Cross Search
[Xue, Chun-Ying]‘s Articles
[Li, De-Zhu]‘s Articles
[Wang, Qing-Zhong]‘s Articles
Related Copyright Policies
Null
Social Bookmarking
Add to CiteULike Add to Connotea Add to Del.icio.us Add to Digg Add to Reddit
文件名: PLANTA MEDICA75 873-875.pdf
格式: Adobe PDF
此文件暂不支持浏览
所有评论 (0)
暂无评论
 
评注功能仅针对注册用户开放,请您登录
您对该条目有什么异议,请填写以下表单,管理员会尽快联系您。
内 容:
Email:  *
单位:
验证码:   刷新
您在IR的使用过程中有什么好的想法或者建议可以反馈给我们。
标 题:
 *
内 容:
Email:  *
验证码:   刷新

Items in IR are protected by copyright, with all rights reserved, unless otherwise indicated.

 

 

Valid XHTML 1.0!
Copyright © 2007-2017  中国科学院昆明植物研究所 - Feedback
Powered by CSpace