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Physical mapping of the 18S-26S rDNA by fluorescent in situ hybridization (FISH) in Camellia reticulata polyploid complex (Theaceae)
Gu, ZJ; Hua, X
2003-02-01
Source PublicationPLANT SCIENCE
ISSN0168-9452
Volume164Issue:2Pages:279-285
AbstractPhysical maps of the 18S-26S rDNA ribosomal RNA genes (rDNA) were generated by fluorescent in situ hybridization (FISH) for Camellia reticulata polyploid complex, including three types of ploidy of C reticulata and its related species, C. japonica, C. yunnanensis, C pitardii and C saluenensis. The advanced method was used for preparing chromosome spreads. Eight, 12 and 18 rDNA sites were observed on the genomes of diploid, tetraploid and hexaploid C. reticulata. Eight, four, five, and four rDNA loci located on the chromosomes of C pitardii, C. japonica, C saluenensis and C yunnanensis respectively. The number and position of rDNA sites in these species were compared for analysis. The results support some of the earlier phylogenetic speculation about this complex and suggest that some chromosomal structural rearrangements have occurred. The multiplicity, variation in size and site numbers between polyploid C reticulata and its putative diploid ancestors indicate that the behavior of rDNA loci in the complex is considerably more complex than previously envisioned. (C) 2002 Elsevier Science Ireland Ltd. All rights reserved.
Keyword18s-26s Rdna Camellia Fluorescent In Situ Hybridization (Fish) Physical Mapping
Subject AreaBiochemistry & Molecular Biology ; Plant Sciences
Indexed BySCI
Language英语
WOS Research AreaBiochemistry & Molecular Biology ; Plant Sciences
WOS SubjectBiochemistry & Molecular Biology ; Plant Sciences
WOS IDWOS:000180838400017
Citation statistics
Cited Times:19[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.kib.ac.cn/handle/151853/13557
Collection离退休
AffiliationChinese Acad Sci, Kunming Inst Bot, Kunming 650204, Yunnan, Peoples R China
Recommended Citation
GB/T 7714
Gu, ZJ,Hua, X. Physical mapping of the 18S-26S rDNA by fluorescent in situ hybridization (FISH) in Camellia reticulata polyploid complex (Theaceae)[J]. PLANT SCIENCE,2003,164(2):279-285.
APA Gu, ZJ,&Hua, X.(2003).Physical mapping of the 18S-26S rDNA by fluorescent in situ hybridization (FISH) in Camellia reticulata polyploid complex (Theaceae).PLANT SCIENCE,164(2),279-285.
MLA Gu, ZJ,et al."Physical mapping of the 18S-26S rDNA by fluorescent in situ hybridization (FISH) in Camellia reticulata polyploid complex (Theaceae)".PLANT SCIENCE 164.2(2003):279-285.
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