Physical mapping of the 18S-26S rDNA by fluorescent in situ hybridization (FISH) in Camellia reticulata polyploid complex (Theaceae)

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	Physical mapping of the 18S-26S rDNA by fluorescent in situ hybridization (FISH) in Camellia reticulata polyploid complex (Theaceae)
	Gu, ZJ; Hua, X
	2003-02-01
发表期刊	PLANT SCIENCE
ISSN	0168-9452
卷号	164 期号:2 页码:279-285
摘要	Physical maps of the 18S-26S rDNA ribosomal RNA genes (rDNA) were generated by fluorescent in situ hybridization (FISH) for Camellia reticulata polyploid complex, including three types of ploidy of C reticulata and its related species, C. japonica, C. yunnanensis, C pitardii and C saluenensis. The advanced method was used for preparing chromosome spreads. Eight, 12 and 18 rDNA sites were observed on the genomes of diploid, tetraploid and hexaploid C. reticulata. Eight, four, five, and four rDNA loci located on the chromosomes of C pitardii, C. japonica, C saluenensis and C yunnanensis respectively. The number and position of rDNA sites in these species were compared for analysis. The results support some of the earlier phylogenetic speculation about this complex and suggest that some chromosomal structural rearrangements have occurred. The multiplicity, variation in size and site numbers between polyploid C reticulata and its putative diploid ancestors indicate that the behavior of rDNA loci in the complex is considerably more complex than previously envisioned. (C) 2002 Elsevier Science Ireland Ltd. All rights reserved.
关键词	18s-26s Rdna Camellia Fluorescent In Situ Hybridization (Fish) Physical Mapping
学科领域	Biochemistry & Molecular Biology ; Plant Sciences
收录类别	SCI
语种	英语
WOS记录号	WOS:000180838400017
引用统计
文献类型	期刊论文
条目标识符	http://ir.kib.ac.cn/handle/151853/13557
专题	离退休
作者单位	Chinese Acad Sci, Kunming Inst Bot, Kunming 650204, Yunnan, Peoples R China
推荐引用方式 GB/T 7714	Gu, ZJ,Hua, X. Physical mapping of the 18S-26S rDNA by fluorescent in situ hybridization (FISH) in Camellia reticulata polyploid complex (Theaceae)[J]. PLANT SCIENCE,2003,164(2):279-285.
APA	Gu, ZJ,&Hua, X.(2003).Physical mapping of the 18S-26S rDNA by fluorescent in situ hybridization (FISH) in Camellia reticulata polyploid complex (Theaceae).PLANT SCIENCE,164(2),279-285.
MLA	Gu, ZJ,et al."Physical mapping of the 18S-26S rDNA by fluorescent in situ hybridization (FISH) in Camellia reticulata polyploid complex (Theaceae)".PLANT SCIENCE 164.2(2003):279-285.

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