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题名: Physical mapping of the 18S-26S rDNA by fluorescent in situ hybridization (FISH) in Camellia reticulata polyploid complex (Theaceae)
作者: Gu, ZJ; Hua, X
刊名: PLANT SCIENCE
关键词: 18S-26S rDNA ; Camellia ; fluorescent in situ hybridization (FISH) ; physical mapping
英文摘要: Physical maps of the 18S-26S rDNA ribosomal RNA genes (rDNA) were generated by fluorescent in situ hybridization (FISH) for Camellia reticulata polyploid complex, including three types of ploidy of C reticulata and its related species, C. japonica, C. yunnanensis, C pitardii and C saluenensis. The advanced method was used for preparing chromosome spreads. Eight, 12 and 18 rDNA sites were observed on the genomes of diploid, tetraploid and hexaploid C. reticulata. Eight, four, five, and four rDNA loci located on the chromosomes of C pitardii, C. japonica, C saluenensis and C yunnanensis respectively. The number and position of rDNA sites in these species were compared for analysis. The results support some of the earlier phylogenetic speculation about this complex and suggest that some chromosomal structural rearrangements have occurred. The multiplicity, variation in size and site numbers between polyploid C reticulata and its putative diploid ancestors indicate that the behavior of rDNA loci in the complex is considerably more complex than previously envisioned. (C) 2002 Elsevier Science Ireland Ltd. All rights reserved.
出版日期: 2003-02-01
卷号: 164, 期号:2, 页码:279-285
语种: 英语
收录类别: SCI
学科分类: Biochemistry & Molecular Biology; Plant Sciences
ISSN号: 0168-9452
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内容类型: 期刊论文
URI标识: http://ir.kib.ac.cn/handle/151853/13557
Appears in Collections:离退休_期刊论文

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作者单位: 1.Chinese Acad Sci, Kunming Inst Bot, Kunming 650204, Yunnan, Peoples R China

Recommended Citation:
Gu, ZJ; Hua, X.Physical mapping of the 18S-26S rDNA by fluorescent in situ hybridization (FISH) in Camellia reticulata polyploid complex (Theaceae),PLANT SCIENCE,2003,164(2):279-285
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