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Physical mapping of the 18S-26S rDNA by fluorescent in situ hybridization (FISH) in Camellia reticulata polyploid complex (Theaceae)
Gu, ZJ; Hua, X
2003-02-01
发表期刊PLANT SCIENCE
ISSN0168-9452
卷号164期号:2页码:279-285
摘要Physical maps of the 18S-26S rDNA ribosomal RNA genes (rDNA) were generated by fluorescent in situ hybridization (FISH) for Camellia reticulata polyploid complex, including three types of ploidy of C reticulata and its related species, C. japonica, C. yunnanensis, C pitardii and C saluenensis. The advanced method was used for preparing chromosome spreads. Eight, 12 and 18 rDNA sites were observed on the genomes of diploid, tetraploid and hexaploid C. reticulata. Eight, four, five, and four rDNA loci located on the chromosomes of C pitardii, C. japonica, C saluenensis and C yunnanensis respectively. The number and position of rDNA sites in these species were compared for analysis. The results support some of the earlier phylogenetic speculation about this complex and suggest that some chromosomal structural rearrangements have occurred. The multiplicity, variation in size and site numbers between polyploid C reticulata and its putative diploid ancestors indicate that the behavior of rDNA loci in the complex is considerably more complex than previously envisioned. (C) 2002 Elsevier Science Ireland Ltd. All rights reserved.
关键词18s-26s Rdna Camellia Fluorescent In Situ Hybridization (Fish) Physical Mapping
学科领域Biochemistry & Molecular Biology ; Plant Sciences
收录类别SCI
语种英语
WOS研究方向Biochemistry & Molecular Biology ; Plant Sciences
WOS类目Biochemistry & Molecular Biology ; Plant Sciences
WOS记录号WOS:000180838400017
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文献类型期刊论文
条目标识符http://ir.kib.ac.cn/handle/151853/13557
专题离退休
作者单位Chinese Acad Sci, Kunming Inst Bot, Kunming 650204, Yunnan, Peoples R China
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Gu, ZJ,Hua, X. Physical mapping of the 18S-26S rDNA by fluorescent in situ hybridization (FISH) in Camellia reticulata polyploid complex (Theaceae)[J]. PLANT SCIENCE,2003,164(2):279-285.
APA Gu, ZJ,&Hua, X.(2003).Physical mapping of the 18S-26S rDNA by fluorescent in situ hybridization (FISH) in Camellia reticulata polyploid complex (Theaceae).PLANT SCIENCE,164(2),279-285.
MLA Gu, ZJ,et al."Physical mapping of the 18S-26S rDNA by fluorescent in situ hybridization (FISH) in Camellia reticulata polyploid complex (Theaceae)".PLANT SCIENCE 164.2(2003):279-285.
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