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Crystal structure of pokeweed antiviral protein with well-defined sugars from seeds at 1.8 angstrom resolution
Zeng, ZH; He, XL; Li, HM; Hu, Z; Wang, DC
2003-02-01
发表期刊JOURNAL OF STRUCTURAL BIOLOGY
ISSN1047-8477
卷号141期号:2页码:171-178
摘要The crystal structure of pokeweed antiviral protein from seeds of Phytolacca americana (PAP-S) was solved at 1.8 Angstrom. PAP-S is a one-chain ribosome-inactivating protein (RIP) and distinctively contains three well-defined N-acetylglucosamines, each covalently linked to an asparagine residue at positions, 10, 44, and 255, respectively. The high-resolution structure clearly shows the three mono-sugars to have either an alpha- or a beta-conformation. Two of sugars are located on the same side of the molecule with the active pocket. Except one hydrogen bond, there are no intermolecular interactions between the polypeptide chain and the sugars. Instead the sugar conformations appear to be stabilized by intermolecular interactions. The sugar structure defined at high resolution provides a structural basis for understanding their possible biological activity. The structural comparisons of PAP-S with other PAPs reveal that the major disparity of these homologous molecules is the different charge distribution on the upper right side of the front side near the active pocket. Based on the available structure of the 50S ribosomal subunit, the possible interactions between PAPs and the ribosome are discussed. (C) 2002 Elsevier Science (USA). All rights reserved.
关键词Pokeweed Antiviral Protein Ribosome-inactivating Protein N-acetylglucosamine Crystal Structure
学科领域Biochemistry & Molecular Biology ; Biophysics ; Cell Biology
DOI10.1016/S1047-8477(02)00580-4
收录类别SCI
语种英语
WOS研究方向Biochemistry & Molecular Biology ; Biophysics ; Cell Biology
WOS类目Biochemistry & Molecular Biology ; Biophysics ; Cell Biology
WOS记录号WOS:000181702600009
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文献类型期刊论文
条目标识符http://ir.kib.ac.cn/handle/151853/13541
专题离退休
作者单位1.Chinese Acad Sci, Inst Biophys, Ctr Biol Mol, Beijing 100101, Peoples R China
2.Chinese Acad Sci, Kunming Inst Bot, Kunming 650204, Peoples R China
3.Chinese Acad Sci, Natl Lab Micrograv, Beijing, Peoples R China
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GB/T 7714
Zeng, ZH,He, XL,Li, HM,et al. Crystal structure of pokeweed antiviral protein with well-defined sugars from seeds at 1.8 angstrom resolution[J]. JOURNAL OF STRUCTURAL BIOLOGY,2003,141(2):171-178.
APA Zeng, ZH,He, XL,Li, HM,Hu, Z,&Wang, DC.(2003).Crystal structure of pokeweed antiviral protein with well-defined sugars from seeds at 1.8 angstrom resolution.JOURNAL OF STRUCTURAL BIOLOGY,141(2),171-178.
MLA Zeng, ZH,et al."Crystal structure of pokeweed antiviral protein with well-defined sugars from seeds at 1.8 angstrom resolution".JOURNAL OF STRUCTURAL BIOLOGY 141.2(2003):171-178.
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