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题名: Crystal structure of pokeweed antiviral protein with well-defined sugars from seeds at 1.8 angstrom resolution
作者: Zeng, ZH; He, XL; Li, HM; Hu, Z; Wang, DC
刊名: JOURNAL OF STRUCTURAL BIOLOGY
关键词: pokeweed antiviral protein ; ribosome-inactivating protein ; N-acetylglucosamine ; crystal structure
英文摘要: The crystal structure of pokeweed antiviral protein from seeds of Phytolacca americana (PAP-S) was solved at 1.8 Angstrom. PAP-S is a one-chain ribosome-inactivating protein (RIP) and distinctively contains three well-defined N-acetylglucosamines, each covalently linked to an asparagine residue at positions, 10, 44, and 255, respectively. The high-resolution structure clearly shows the three mono-sugars to have either an alpha- or a beta-conformation. Two of sugars are located on the same side of the molecule with the active pocket. Except one hydrogen bond, there are no intermolecular interactions between the polypeptide chain and the sugars. Instead the sugar conformations appear to be stabilized by intermolecular interactions. The sugar structure defined at high resolution provides a structural basis for understanding their possible biological activity. The structural comparisons of PAP-S with other PAPs reveal that the major disparity of these homologous molecules is the different charge distribution on the upper right side of the front side near the active pocket. Based on the available structure of the 50S ribosomal subunit, the possible interactions between PAPs and the ribosome are discussed. (C) 2002 Elsevier Science (USA). All rights reserved.
出版日期: 2003-02-01
卷号: 141, 期号:2, 页码:171-178
DOI标识: 10.1016/S1047-8477(02)00580-4
语种: 英语
ISSN号: 1047-8477
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内容类型: 期刊论文
URI标识: http://ir.kib.ac.cn/handle/151853/13541
Appears in Collections:离退休_期刊论文

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作者单位: 1.Chinese Acad Sci, Inst Biophys, Ctr Biol Mol, Beijing 100101, Peoples R China
2.Chinese Acad Sci, Kunming Inst Bot, Kunming 650204, Peoples R China
3.Chinese Acad Sci, Natl Lab Micrograv, Beijing, Peoples R China

Recommended Citation:
Zeng, ZH; He, XL; Li, HM; Hu, Z; Wang, DC.Crystal structure of pokeweed antiviral protein with well-defined sugars from seeds at 1.8 angstrom resolution,JOURNAL OF STRUCTURAL BIOLOGY,2003,141(2):171-178
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