Productions of taxol and related taxanes by cell suspension cultures of Taxus yunnanensis

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	Productions of taxol and related taxanes by cell suspension cultures of Taxus yunnanensis
	Hu, YM ; Gan, FY ; Lu, CH ; Ding, HS ; Shen, YM
通讯作者	yshen@public.km.yn.cn
	2003-03-01
发表期刊	ACTA BOTANICA SINICA
ISSN	1672-6650
卷号	45 期号:3 页码:373-378
摘要	A high taxol yield cell line of Taxus yunnanensis Cheng et L. K. Fu keeps a high taxol-producing level after successive subcultures for more than eight years. In this study, eight taxanes were isolated from the suspension cell cultures of this cell line. Based on NMR and MS analyses, and comparison with literature data and standards, their structures were determined to be 2alpha, 5alpha, 10beta-triacetoxy-14beta-proplonyloxy-4(20), 11-taxadiene (1), 2alpha, 5alpha, 10beta-triacetoxy-14beta-(2'-methyl)-butyryloxy-4(20), 11-taxadiene (2), 2alpha, 5alpha, 10beta-14beta-tetra-acetoxy-4 (20), 11-taxadiene (3, taxuyunnanine C), 2alpha, 5alpha, 10beta-triacetoxy-14beta-(2'-methyl-3'-hydroxy)-butyryloxy-4(20), 11-taxadiene (4, yunnanxane) and its 3'-epimer (5), baccatin IV (6), baccatin III (7) and taxol (8), respectively. Among those compounds, 3, 5, 6 and 7 were reported to be isolated from the suspension cell cultures of T. yunnanensis for the first time. TLC and HPLC analyses indicated that the chemical constituents of the culture solution were similar to those of cultured cells. Moreover, the highest taxol content of this cell line reached 0.3% and the cell line could be applied for a large-scale culture.
关键词	Taxus Yunnanensis Cell Suspension Cultures Taxol Taxanes
学科领域	Biochemistry & Molecular Biology ; Plant Sciences
收录类别	SCI
语种	英语
WOS记录号	WOS:000181805100020
引用统计
文献类型	期刊论文
条目标识符	http://ir.kib.ac.cn/handle/151853/13513
专题	离退休
作者单位	1.Chinese Acad Sci, Kunming Inst Bot, Kunming 650204, Peoples R China 2.Peking Univ, Natl Lab Prot Engn & Plant Genet Engn, Beijing 100871, Peoples R China 3.Fujian Coll Tradit Chinese Med, Fuzhou 350003, Peoples R China
推荐引用方式 GB/T 7714	Hu, YM,Gan, FY,Lu, CH,et al. Productions of taxol and related taxanes by cell suspension cultures of Taxus yunnanensis[J]. ACTA BOTANICA SINICA,2003,45(3):373-378.
APA	Hu, YM,Gan, FY,Lu, CH,Ding, HS,&Shen, YM.(2003).Productions of taxol and related taxanes by cell suspension cultures of Taxus yunnanensis.ACTA BOTANICA SINICA,45(3),373-378.
MLA	Hu, YM,et al."Productions of taxol and related taxanes by cell suspension cultures of Taxus yunnanensis".ACTA BOTANICA SINICA 45.3(2003):373-378.

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