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Productions of taxol and related taxanes by cell suspension cultures of Taxus yunnanensis
Hu, YM; Gan, FY; Lu, CH; Ding, HS; Shen, YM
2003-03-01
发表期刊ACTA BOTANICA SINICA
ISSN1672-6650
卷号45期号:3页码:373-378
摘要A high taxol yield cell line of Taxus yunnanensis Cheng et L. K. Fu keeps a high taxol-producing level after successive subcultures for more than eight years. In this study, eight taxanes were isolated from the suspension cell cultures of this cell line. Based on NMR and MS analyses, and comparison with literature data and standards, their structures were determined to be 2alpha, 5alpha, 10beta-triacetoxy-14beta-proplonyloxy-4(20), 11-taxadiene (1), 2alpha, 5alpha, 10beta-triacetoxy-14beta-(2'-methyl)-butyryloxy-4(20), 11-taxadiene (2), 2alpha, 5alpha, 10beta-14beta-tetra-acetoxy-4 (20), 11-taxadiene (3, taxuyunnanine C), 2alpha, 5alpha, 10beta-triacetoxy-14beta-(2'-methyl-3'-hydroxy)-butyryloxy-4(20), 11-taxadiene (4, yunnanxane) and its 3'-epimer (5), baccatin IV (6), baccatin III (7) and taxol (8), respectively. Among those compounds, 3, 5, 6 and 7 were reported to be isolated from the suspension cell cultures of T. yunnanensis for the first time. TLC and HPLC analyses indicated that the chemical constituents of the culture solution were similar to those of cultured cells. Moreover, the highest taxol content of this cell line reached 0.3% and the cell line could be applied for a large-scale culture.
关键词Taxus Yunnanensis Cell Suspension Cultures Taxol Taxanes
学科领域Biochemistry & Molecular Biology ; Plant Sciences
收录类别SCI
语种英语
WOS研究方向Biochemistry & Molecular Biology ; Plant Sciences
WOS类目Biochemistry & Molecular Biology ; Plant Sciences
WOS记录号WOS:000181805100020
引用统计
被引频次:7[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.kib.ac.cn/handle/151853/13513
专题离退休
作者单位1.Chinese Acad Sci, Kunming Inst Bot, Kunming 650204, Peoples R China
2.Peking Univ, Natl Lab Prot Engn & Plant Genet Engn, Beijing 100871, Peoples R China
3.Fujian Coll Tradit Chinese Med, Fuzhou 350003, Peoples R China
推荐引用方式
GB/T 7714
Hu, YM,Gan, FY,Lu, CH,et al. Productions of taxol and related taxanes by cell suspension cultures of Taxus yunnanensis[J]. ACTA BOTANICA SINICA,2003,45(3):373-378.
APA Hu, YM,Gan, FY,Lu, CH,Ding, HS,&Shen, YM.(2003).Productions of taxol and related taxanes by cell suspension cultures of Taxus yunnanensis.ACTA BOTANICA SINICA,45(3),373-378.
MLA Hu, YM,et al."Productions of taxol and related taxanes by cell suspension cultures of Taxus yunnanensis".ACTA BOTANICA SINICA 45.3(2003):373-378.
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