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Productions of taxol and related taxanes by cell suspension cultures of Taxus yunnanensis | |
Hu, YM; Gan, FY; Lu, CH; Ding, HS; Shen, YM | |
通讯作者 | yshen@public.km.yn.cn |
2003-03-01 | |
发表期刊 | ACTA BOTANICA SINICA
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ISSN | 1672-6650 |
卷号 | 45期号:3页码:373-378 |
摘要 | A high taxol yield cell line of Taxus yunnanensis Cheng et L. K. Fu keeps a high taxol-producing level after successive subcultures for more than eight years. In this study, eight taxanes were isolated from the suspension cell cultures of this cell line. Based on NMR and MS analyses, and comparison with literature data and standards, their structures were determined to be 2alpha, 5alpha, 10beta-triacetoxy-14beta-proplonyloxy-4(20), 11-taxadiene (1), 2alpha, 5alpha, 10beta-triacetoxy-14beta-(2'-methyl)-butyryloxy-4(20), 11-taxadiene (2), 2alpha, 5alpha, 10beta-14beta-tetra-acetoxy-4 (20), 11-taxadiene (3, taxuyunnanine C), 2alpha, 5alpha, 10beta-triacetoxy-14beta-(2'-methyl-3'-hydroxy)-butyryloxy-4(20), 11-taxadiene (4, yunnanxane) and its 3'-epimer (5), baccatin IV (6), baccatin III (7) and taxol (8), respectively. Among those compounds, 3, 5, 6 and 7 were reported to be isolated from the suspension cell cultures of T. yunnanensis for the first time. TLC and HPLC analyses indicated that the chemical constituents of the culture solution were similar to those of cultured cells. Moreover, the highest taxol content of this cell line reached 0.3% and the cell line could be applied for a large-scale culture. |
关键词 | Taxus Yunnanensis Cell Suspension Cultures Taxol Taxanes |
学科领域 | Biochemistry & Molecular Biology ; Plant Sciences |
收录类别 | SCI |
语种 | 英语 |
WOS记录号 | WOS:000181805100020 |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | http://ir.kib.ac.cn/handle/151853/13513 |
专题 | 离退休 |
作者单位 | 1.Chinese Acad Sci, Kunming Inst Bot, Kunming 650204, Peoples R China 2.Peking Univ, Natl Lab Prot Engn & Plant Genet Engn, Beijing 100871, Peoples R China 3.Fujian Coll Tradit Chinese Med, Fuzhou 350003, Peoples R China |
推荐引用方式 GB/T 7714 | Hu, YM,Gan, FY,Lu, CH,et al. Productions of taxol and related taxanes by cell suspension cultures of Taxus yunnanensis[J]. ACTA BOTANICA SINICA,2003,45(3):373-378. |
APA | Hu, YM,Gan, FY,Lu, CH,Ding, HS,&Shen, YM.(2003).Productions of taxol and related taxanes by cell suspension cultures of Taxus yunnanensis.ACTA BOTANICA SINICA,45(3),373-378. |
MLA | Hu, YM,et al."Productions of taxol and related taxanes by cell suspension cultures of Taxus yunnanensis".ACTA BOTANICA SINICA 45.3(2003):373-378. |
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