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题名: Productions of taxol and related taxanes by cell suspension cultures of Taxus yunnanensis
作者: Hu, YM; Gan, FY; Lu, CH; Ding, HS; Shen, YM
刊名: ACTA BOTANICA SINICA
关键词: Taxus yunnanensis ; cell suspension cultures ; taxol ; taxanes
英文摘要: A high taxol yield cell line of Taxus yunnanensis Cheng et L. K. Fu keeps a high taxol-producing level after successive subcultures for more than eight years. In this study, eight taxanes were isolated from the suspension cell cultures of this cell line. Based on NMR and MS analyses, and comparison with literature data and standards, their structures were determined to be 2alpha, 5alpha, 10beta-triacetoxy-14beta-proplonyloxy-4(20), 11-taxadiene (1), 2alpha, 5alpha, 10beta-triacetoxy-14beta-(2'-methyl)-butyryloxy-4(20), 11-taxadiene (2), 2alpha, 5alpha, 10beta-14beta-tetra-acetoxy-4 (20), 11-taxadiene (3, taxuyunnanine C), 2alpha, 5alpha, 10beta-triacetoxy-14beta-(2'-methyl-3'-hydroxy)-butyryloxy-4(20), 11-taxadiene (4, yunnanxane) and its 3'-epimer (5), baccatin IV (6), baccatin III (7) and taxol (8), respectively. Among those compounds, 3, 5, 6 and 7 were reported to be isolated from the suspension cell cultures of T. yunnanensis for the first time. TLC and HPLC analyses indicated that the chemical constituents of the culture solution were similar to those of cultured cells. Moreover, the highest taxol content of this cell line reached 0.3% and the cell line could be applied for a large-scale culture.
出版日期: 2003-03-01
卷号: 45, 期号:3, 页码:373-378
语种: 英语
收录类别: SCI
学科分类: Biochemistry & Molecular Biology; Plant Sciences
ISSN号: 1672-6650
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.kib.ac.cn/handle/151853/13513
Appears in Collections:离退休_期刊论文

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作者单位: 1.Chinese Acad Sci, Kunming Inst Bot, Kunming 650204, Peoples R China
2.Peking Univ, Natl Lab Prot Engn & Plant Genet Engn, Beijing 100871, Peoples R China
3.Fujian Coll Tradit Chinese Med, Fuzhou 350003, Peoples R China

Recommended Citation:
Hu, YM; Gan, FY; Lu, CH; Ding, HS; Shen, YM.Productions of taxol and related taxanes by cell suspension cultures of Taxus yunnanensis,ACTA BOTANICA SINICA,2003,45(3):373-378
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