KIB OpenIR  > 离退休
Productions of taxol and related taxanes by cell suspension cultures of Taxus yunnanensis
Hu, YM; Gan, FY; Lu, CH; Ding, HS; Shen, YM
Corresponding Authoryshen@public.km.yn.cn
2003-03-01
Source PublicationACTA BOTANICA SINICA
ISSN1672-6650
Volume45Issue:3Pages:373-378
AbstractA high taxol yield cell line of Taxus yunnanensis Cheng et L. K. Fu keeps a high taxol-producing level after successive subcultures for more than eight years. In this study, eight taxanes were isolated from the suspension cell cultures of this cell line. Based on NMR and MS analyses, and comparison with literature data and standards, their structures were determined to be 2alpha, 5alpha, 10beta-triacetoxy-14beta-proplonyloxy-4(20), 11-taxadiene (1), 2alpha, 5alpha, 10beta-triacetoxy-14beta-(2'-methyl)-butyryloxy-4(20), 11-taxadiene (2), 2alpha, 5alpha, 10beta-14beta-tetra-acetoxy-4 (20), 11-taxadiene (3, taxuyunnanine C), 2alpha, 5alpha, 10beta-triacetoxy-14beta-(2'-methyl-3'-hydroxy)-butyryloxy-4(20), 11-taxadiene (4, yunnanxane) and its 3'-epimer (5), baccatin IV (6), baccatin III (7) and taxol (8), respectively. Among those compounds, 3, 5, 6 and 7 were reported to be isolated from the suspension cell cultures of T. yunnanensis for the first time. TLC and HPLC analyses indicated that the chemical constituents of the culture solution were similar to those of cultured cells. Moreover, the highest taxol content of this cell line reached 0.3% and the cell line could be applied for a large-scale culture.
KeywordTaxus Yunnanensis Cell Suspension Cultures Taxol Taxanes
Subject AreaBiochemistry & Molecular Biology ; Plant Sciences
Indexed BySCI
Language英语
WOS Research AreaBiochemistry & Molecular Biology ; Plant Sciences
WOS SubjectBiochemistry & Molecular Biology ; Plant Sciences
WOS IDWOS:000181805100020
Citation statistics
Cited Times:8[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.kib.ac.cn/handle/151853/13513
Collection离退休
Affiliation1.Chinese Acad Sci, Kunming Inst Bot, Kunming 650204, Peoples R China
2.Peking Univ, Natl Lab Prot Engn & Plant Genet Engn, Beijing 100871, Peoples R China
3.Fujian Coll Tradit Chinese Med, Fuzhou 350003, Peoples R China
Recommended Citation
GB/T 7714
Hu, YM,Gan, FY,Lu, CH,et al. Productions of taxol and related taxanes by cell suspension cultures of Taxus yunnanensis[J]. ACTA BOTANICA SINICA,2003,45(3):373-378.
APA Hu, YM,Gan, FY,Lu, CH,Ding, HS,&Shen, YM.(2003).Productions of taxol and related taxanes by cell suspension cultures of Taxus yunnanensis.ACTA BOTANICA SINICA,45(3),373-378.
MLA Hu, YM,et al."Productions of taxol and related taxanes by cell suspension cultures of Taxus yunnanensis".ACTA BOTANICA SINICA 45.3(2003):373-378.
Files in This Item: Download All
File Name/Size DocType Version Access License
ACTA BOTANICA SINICA(5324KB) 开放获取--View Download
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Hu, YM]'s Articles
[Gan, FY]'s Articles
[Lu, CH]'s Articles
Baidu academic
Similar articles in Baidu academic
[Hu, YM]'s Articles
[Gan, FY]'s Articles
[Lu, CH]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Hu, YM]'s Articles
[Gan, FY]'s Articles
[Lu, CH]'s Articles
Terms of Use
No data!
Social Bookmark/Share
File name: ACTA BOTANICA SINICA45 373-378.pdf
Format: Adobe PDF
This file does not support browsing at this time
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.