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High universality of matK primers for barcoding gymnosperms
Li, Yan1,2; Gao, Lian-Ming1; Poudel, Ram C.1,3,4; Li, De-Zhu1,3; Forrest, Alan5
2011-05-01
发表期刊JOURNAL OF SYSTEMATICS AND EVOLUTION
卷号49期号:3页码:169-175
摘要DNA barcoding is a tool to provide rapid and accurate taxonomic identification using a standard DNA region. A two-marker combination of matK+rbcL was formally proposed as the core barcode for land plants by the Consortium for the Barcode of Life Plant Working Group. However, there are currently no barcoding primers for matK showing high universality in gymnosperms. We used 57 gymnosperm species representing 40 genera, 11 families and four subclasses to evaluate the universality of nine candidate matK primers and one rbcL primer in this study. Primer (1F/724R) of rbcL is proposed here as a universal primer for gymnosperms due to high universality. One of the nine candidate matK primers (Gym_F1A/Gym_R1A) is proposed as the best "universal" matK primer for gymnosperms because of high polymerase chain reaction success and routine generation of high quality bidirectional sequences. A specific matK primer for Ephedra was newly designed in this study, which performed well on the sampled species. The primers proposed here for rbcL and matK can be easily and successfully amplified for most gymnosperms.
关键词Dna Barcoding Matk Gymnosperm Primer Universality
资助信息Chinese Academy of Sciences [2009-LSF-GBOWS-01, 9223111W1]; Talent Project of Yunnan Province, China [2008PY064]; Gordon and Betty Moore Foundation [2535]
学科领域Plant Sciences
DOI10.1111/j.1759-6831.2011.00128.x
收录类别SCI
语种英语
WOS研究方向Plant Sciences
WOS类目Plant Sciences
WOS记录号WOS:000291236500002
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文献类型期刊论文
条目标识符http://ir.kib.ac.cn/handle/151853/11239
专题中国西南野生生物种质资源库
作者单位1.Chinese Acad Sci, Key Lab Biodivers & Biogeog, Kunming Inst Bot, Kunming 650204, Peoples R China
2.Yunnan Acad Agr Sci, Inst Alpine Econ Plants, Lijiang 674100, Peoples R China
3.Chinese Acad Sci, Germplasm Bank Wild Species SW China, Kunming Inst Bot, Kunming 650204, Peoples R China
4.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China
5.Royal Bot Garden Edinburgh, Edinburgh EH3 5LR, Midlothian, Scotland
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Li, Yan,Gao, Lian-Ming,Poudel, Ram C.,et al. High universality of matK primers for barcoding gymnosperms[J]. JOURNAL OF SYSTEMATICS AND EVOLUTION,2011,49(3):169-175.
APA Li, Yan,Gao, Lian-Ming,Poudel, Ram C.,Li, De-Zhu,&Forrest, Alan.(2011).High universality of matK primers for barcoding gymnosperms.JOURNAL OF SYSTEMATICS AND EVOLUTION,49(3),169-175.
MLA Li, Yan,et al."High universality of matK primers for barcoding gymnosperms".JOURNAL OF SYSTEMATICS AND EVOLUTION 49.3(2011):169-175.
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