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题名: High universality of matK primers for barcoding gymnosperms
作者: null(李艳)1, 2; null(高连明)1; Poudel, Ram C.1, 3, 4; null(李德铢)1, 3; Forrest, Alan5
刊名: JOURNAL OF SYSTEMATICS AND EVOLUTION
关键词: DNA barcoding ; matK ; gymnosperm ; primer universality
英文摘要: DNA barcoding is a tool to provide rapid and accurate taxonomic identification using a standard DNA region. A two-marker combination of matK+rbcL was formally proposed as the core barcode for land plants by the Consortium for the Barcode of Life Plant Working Group. However, there are currently no barcoding primers for matK showing high universality in gymnosperms. We used 57 gymnosperm species representing 40 genera, 11 families and four subclasses to evaluate the universality of nine candidate matK primers and one rbcL primer in this study. Primer (1F/724R) of rbcL is proposed here as a universal primer for gymnosperms due to high universality. One of the nine candidate matK primers (Gym_F1A/Gym_R1A) is proposed as the best "universal" matK primer for gymnosperms because of high polymerase chain reaction success and routine generation of high quality bidirectional sequences. A specific matK primer for Ephedra was newly designed in this study, which performed well on the sampled species. The primers proposed here for rbcL and matK can be easily and successfully amplified for most gymnosperms.
出版日期: 2011-05-01
卷号: 49, 期号:3, 页码:169-175
DOI标识: 10.1111/j.1759-6831.2011.00128.x
语种: 英语
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内容类型: 期刊论文
URI标识: http://ir.kib.ac.cn/handle/151853/11239
Appears in Collections:中国西南野生生物种质资源库_期刊论文

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作者单位: 1.Chinese Acad Sci, Key Lab Biodivers & Biogeog, Kunming Inst Bot, Kunming 650204, Peoples R China
2.Yunnan Acad Agr Sci, Inst Alpine Econ Plants, Lijiang 674100, Peoples R China
3.Chinese Acad Sci, Germplasm Bank Wild Species SW China, Kunming Inst Bot, Kunming 650204, Peoples R China
4.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China
5.Royal Bot Garden Edinburgh, Edinburgh EH3 5LR, Midlothian, Scotland

Recommended Citation:
Li, Y; Gao, LM; Poudel, RC; Li, DZ; Forrest, A.High universality of matK primers for barcoding gymnosperms,JOURNAL OF SYSTEMATICS AND EVOLUTION,2011,49(3):169-175
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