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Efficiency of DNA barcodes for species delimitation: A case in Pterygiella Oliv. (Orobanchaceae)
Dong, Li-Na1,2,3; Wortley, Alexandra H.4; Wang, Hong1,2; Li, De-Zhu1,2; Lu, Lu1,2
2011-05-01
发表期刊JOURNAL OF SYSTEMATICS AND EVOLUTION
卷号49期号:3页码:189-202
摘要DNA barcoding is becoming an increasingly popular means to identify species. The obscure discrimination in the genus Pterygiella calls into question the re-assessment of the criterion for species delimitation. We collected 20 individuals, representing all five described species of this genus in its distributional range. The aim was to use three proposed barcode DNA regions (rbcL, matK, and ITS) to diagnose Pterygiella species, and examine which barcode is more suitable for discerning the congeneric and related species. The results showed that the core barcodes matK and rbcL were comparatively less effective. However, the ITS region, especially ITS-1 and ITS-2, successfully identified all species in the genus. Furthermore, the secondary structure of ITS-2 RNA, especially compensatory base changes, appears complementary to classical primary sequence analysis for DNA barcoding.
关键词Compensatory Base Changes (Cbcs) Dna Barcode Its-2 Non-monophyletic Species Pterygiella Oliv. Secondary Structure
资助信息National Natural Science Foundation of China [30970201]; CAS [2009-LSF-GBOWS-01]
学科领域Plant Sciences
DOI10.1111/j.1759-6831.2011.00124.x
收录类别SCI
语种英语
WOS研究方向Plant Sciences
WOS类目Plant Sciences
WOS记录号WOS:000291236500005
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文献类型期刊论文
条目标识符http://ir.kib.ac.cn/handle/151853/11237
专题中国西南野生生物种质资源库
作者单位1.Chinese Acad Sci, Key Lab Biodivers & Biogeog, Germplasm Bank Wild Species, Kunming Inst Bot, Kunming 650204, Yunnan, Peoples R China
2.Chinese Acad Sci, Plant Germplasm & Genom Ctr, Germplasm Bank Wild Species, Kunming Inst Bot, Kunming 650204, Yunnan, Peoples R China
3.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China
4.Royal Bot Garden Edinburgh, Edinburgh EH3 5LR, Midlothian, Scotland
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GB/T 7714
Dong, Li-Na,Wortley, Alexandra H.,Wang, Hong,et al. Efficiency of DNA barcodes for species delimitation: A case in Pterygiella Oliv. (Orobanchaceae)[J]. JOURNAL OF SYSTEMATICS AND EVOLUTION,2011,49(3):189-202.
APA Dong, Li-Na,Wortley, Alexandra H.,Wang, Hong,Li, De-Zhu,&Lu, Lu.(2011).Efficiency of DNA barcodes for species delimitation: A case in Pterygiella Oliv. (Orobanchaceae).JOURNAL OF SYSTEMATICS AND EVOLUTION,49(3),189-202.
MLA Dong, Li-Na,et al."Efficiency of DNA barcodes for species delimitation: A case in Pterygiella Oliv. (Orobanchaceae)".JOURNAL OF SYSTEMATICS AND EVOLUTION 49.3(2011):189-202.
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