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题名: Efficiency of DNA barcodes for species delimitation: A case in Pterygiella Oliv. (Orobanchaceae)
作者: Dong, Li-Na1, 2, 3; Wortley, Alexandra H.4; null(王红)1, 2; null(李德铢)1, 2; Lu, Lu1, 2
刊名: JOURNAL OF SYSTEMATICS AND EVOLUTION
关键词: compensatory base changes (CBCs) ; DNA barcode ; ITS-2 ; non-monophyletic species ; Pterygiella Oliv. ; secondary structure
英文摘要: DNA barcoding is becoming an increasingly popular means to identify species. The obscure discrimination in the genus Pterygiella calls into question the re-assessment of the criterion for species delimitation. We collected 20 individuals, representing all five described species of this genus in its distributional range. The aim was to use three proposed barcode DNA regions (rbcL, matK, and ITS) to diagnose Pterygiella species, and examine which barcode is more suitable for discerning the congeneric and related species. The results showed that the core barcodes matK and rbcL were comparatively less effective. However, the ITS region, especially ITS-1 and ITS-2, successfully identified all species in the genus. Furthermore, the secondary structure of ITS-2 RNA, especially compensatory base changes, appears complementary to classical primary sequence analysis for DNA barcoding.
出版日期: 2011-05-01
卷号: 49, 期号:3, 页码:189-202
DOI标识: 10.1111/j.1759-6831.2011.00124.x
语种: 英语
收录类别: SCI
学科分类: Plant Sciences
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内容类型: 期刊论文
URI标识: http://ir.kib.ac.cn/handle/151853/11237
Appears in Collections:中国西南野生生物种质资源库_期刊论文

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作者单位: 1.Chinese Acad Sci, Key Lab Biodivers & Biogeog, Germplasm Bank Wild Species, Kunming Inst Bot, Kunming 650204, Yunnan, Peoples R China
2.Chinese Acad Sci, Plant Germplasm & Genom Ctr, Germplasm Bank Wild Species, Kunming Inst Bot, Kunming 650204, Yunnan, Peoples R China
3.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China
4.Royal Bot Garden Edinburgh, Edinburgh EH3 5LR, Midlothian, Scotland

Recommended Citation:
Dong, LN; Wortley, AH; Wang, H; Li, DZ; Lu, L.Efficiency of DNA barcodes for species delimitation: A case in Pterygiella Oliv. (Orobanchaceae),JOURNAL OF SYSTEMATICS AND EVOLUTION,2011,49(3):189-202
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