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Efficiency of DNA barcodes for species delimitation: A case in Pterygiella Oliv. (Orobanchaceae)
Dong, Li-Na1,2,3; Wortley, Alexandra H.4; Wang, Hong1,2; Li, De-Zhu1,2; Lu, Lu1,2
2011-05-01
Source PublicationJOURNAL OF SYSTEMATICS AND EVOLUTION
Volume49Issue:3Pages:189-202
AbstractDNA barcoding is becoming an increasingly popular means to identify species. The obscure discrimination in the genus Pterygiella calls into question the re-assessment of the criterion for species delimitation. We collected 20 individuals, representing all five described species of this genus in its distributional range. The aim was to use three proposed barcode DNA regions (rbcL, matK, and ITS) to diagnose Pterygiella species, and examine which barcode is more suitable for discerning the congeneric and related species. The results showed that the core barcodes matK and rbcL were comparatively less effective. However, the ITS region, especially ITS-1 and ITS-2, successfully identified all species in the genus. Furthermore, the secondary structure of ITS-2 RNA, especially compensatory base changes, appears complementary to classical primary sequence analysis for DNA barcoding.
KeywordCompensatory Base Changes (Cbcs) Dna Barcode Its-2 Non-monophyletic Species Pterygiella Oliv. Secondary Structure
Subject AreaPlant Sciences
DOI10.1111/j.1759-6831.2011.00124.x
Indexed BySCI
Language英语
WOS Research AreaPlant Sciences
WOS SubjectPlant Sciences
WOS IDWOS:000291236500005
Citation statistics
Cited Times:14[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.kib.ac.cn/handle/151853/11237
Collection中国西南野生生物种质资源库
Affiliation1.Chinese Acad Sci, Key Lab Biodivers & Biogeog, Germplasm Bank Wild Species, Kunming Inst Bot, Kunming 650204, Yunnan, Peoples R China
2.Chinese Acad Sci, Plant Germplasm & Genom Ctr, Germplasm Bank Wild Species, Kunming Inst Bot, Kunming 650204, Yunnan, Peoples R China
3.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China
4.Royal Bot Garden Edinburgh, Edinburgh EH3 5LR, Midlothian, Scotland
Recommended Citation
GB/T 7714
Dong, Li-Na,Wortley, Alexandra H.,Wang, Hong,et al. Efficiency of DNA barcodes for species delimitation: A case in Pterygiella Oliv. (Orobanchaceae)[J]. JOURNAL OF SYSTEMATICS AND EVOLUTION,2011,49(3):189-202.
APA Dong, Li-Na,Wortley, Alexandra H.,Wang, Hong,Li, De-Zhu,&Lu, Lu.(2011).Efficiency of DNA barcodes for species delimitation: A case in Pterygiella Oliv. (Orobanchaceae).JOURNAL OF SYSTEMATICS AND EVOLUTION,49(3),189-202.
MLA Dong, Li-Na,et al."Efficiency of DNA barcodes for species delimitation: A case in Pterygiella Oliv. (Orobanchaceae)".JOURNAL OF SYSTEMATICS AND EVOLUTION 49.3(2011):189-202.
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