| Isolation and characterization of 15 microsatellite markers from wild tea plant (Camellia taliensis) using FIASCO method | |
Yang, Jun-Bo1,3; Yang, Jing3 ; Li, Hong-Tao3; Zhao, Yu2; Yang, Shi-Xiong1
| |
| 2009-10-01 | |
| 发表期刊 | CONSERVATION GENETICS
 |
| 卷号 | 10期号:5页码:1621-1623 |
| 摘要 | Camellia taliensis is one of the most important wild tea plants in China, especially in Yunnan Province. In this study, we described the development of 15 microsatellite markers from the genome of C. taliensis using the protocol of fast isolation by AFLP of sequences containing repeats (FIASCO). Polymorphism of each locus was assessed in 24 samples collected from six wild populations of C. taliensis. The average allele number of the microsatellites was four per locus, ranging from 2 to 7. The observed and expected heterozygosities varied from 0.076 to 0.5833 and from 0.1560 to 0.6917, respectively. Cross-species amplification in other three tea plants showed eleven of them holding promise for sister species. These polymorphic SSR markers would be useful tools for population genetics studies and assessing genetic variations to establish conservation strategy, molecular identification and molecular breeding on this tea plant and its allied species and varieties in section Thea genus Camellia. |
| 关键词 | Camellia Taliensis Fiasco Microsatellite Markers Polymorphism Tea Plant |
| 学科领域 | Biodiversity Conservation ; Genetics & Heredity |
| DOI | 10.1007/s10592-009-9814-3 |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000270737400077 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.kib.ac.cn/handle/151853/11215 |
| 专题 | 中国西南野生生物种质资源库 |
| 作者单位 | 1.Chinese Acad Sci, Kunming Inst Bot, Key Lab Biodivers & Biogeog, Kunming 650204, Yunnan, Peoples R China 2.Jiangxi Agr Univ, Nanchang, Jiangxi, Peoples R China 3.Chinese Acad Sci, Kunming Inst Bot, SW China Germplasm Bank Wild Species, Kunming 650204, Yunnan, Peoples R China |
| 推荐引用方式 GB/T 7714 | Yang, Jun-Bo,Yang, Jing,Li, Hong-Tao,et al. Isolation and characterization of 15 microsatellite markers from wild tea plant (Camellia taliensis) using FIASCO method[J]. CONSERVATION GENETICS,2009,10(5):1621-1623. |
| APA | Yang, Jun-Bo,Yang, Jing,Li, Hong-Tao,Zhao, Yu,&Yang, Shi-Xiong.(2009).Isolation and characterization of 15 microsatellite markers from wild tea plant (Camellia taliensis) using FIASCO method.CONSERVATION GENETICS,10(5),1621-1623. |
| MLA | Yang, Jun-Bo,et al."Isolation and characterization of 15 microsatellite markers from wild tea plant (Camellia taliensis) using FIASCO method".CONSERVATION GENETICS 10.5(2009):1621-1623. |
| 条目包含的文件 | 下载所有文件 | |||||
| 文件名称/大小 | 文献类型 | 版本类型 | 开放类型 | 使用许可 | ||
| 201109070031.PDF（171KB） | 开放获取 | -- | 浏览 下载 | |||
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。
修改评论