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题名: Isolation and characterization of 15 microsatellite markers from wild tea plant (Camellia taliensis) using FIASCO method
作者: Yang, Jun-Bo1, 3; Yang, Jing3; Li, Hong-Tao3; Zhao, Yu2; Yang, Shi-Xiong1
刊名: CONSERVATION GENETICS
关键词: Camellia taliensis ; FIASCO ; Microsatellite markers ; Polymorphism ; Tea plant
英文摘要: Camellia taliensis is one of the most important wild tea plants in China, especially in Yunnan Province. In this study, we described the development of 15 microsatellite markers from the genome of C. taliensis using the protocol of fast isolation by AFLP of sequences containing repeats (FIASCO). Polymorphism of each locus was assessed in 24 samples collected from six wild populations of C. taliensis. The average allele number of the microsatellites was four per locus, ranging from 2 to 7. The observed and expected heterozygosities varied from 0.076 to 0.5833 and from 0.1560 to 0.6917, respectively. Cross-species amplification in other three tea plants showed eleven of them holding promise for sister species. These polymorphic SSR markers would be useful tools for population genetics studies and assessing genetic variations to establish conservation strategy, molecular identification and molecular breeding on this tea plant and its allied species and varieties in section Thea genus Camellia.
出版日期: 2009-10-01
卷号: 10, 期号:5, 页码:1621-1623
DOI标识: 10.1007/s10592-009-9814-3
语种: 英语
收录类别: SCI
学科分类: Biodiversity Conservation; Genetics & Heredity
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.kib.ac.cn/handle/151853/11215
Appears in Collections:中国西南野生生物种质资源库_期刊论文

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作者单位: 1.Chinese Acad Sci, Kunming Inst Bot, Key Lab Biodivers & Biogeog, Kunming 650204, Yunnan, Peoples R China
2.Jiangxi Agr Univ, Nanchang, Jiangxi, Peoples R China
3.Chinese Acad Sci, Kunming Inst Bot, SW China Germplasm Bank Wild Species, Kunming 650204, Yunnan, Peoples R China

Recommended Citation:
Yang, JB; Yang, J; Li, HT; Zhao, Y; Yang, SX.Isolation and characterization of 15 microsatellite markers from wild tea plant (Camellia taliensis) using FIASCO method,CONSERVATION GENETICS,2009,10(5):1621-1623
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