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Isolation and characterization of 15 microsatellite markers from wild tea plant (Camellia taliensis) using FIASCO method
Yang, Jun-Bo1,3; Yang, Jing3; Li, Hong-Tao3; Zhao, Yu2; Yang, Shi-Xiong1
2009-10-01
发表期刊CONSERVATION GENETICS
卷号10期号:5页码:1621-1623
摘要Camellia taliensis is one of the most important wild tea plants in China, especially in Yunnan Province. In this study, we described the development of 15 microsatellite markers from the genome of C. taliensis using the protocol of fast isolation by AFLP of sequences containing repeats (FIASCO). Polymorphism of each locus was assessed in 24 samples collected from six wild populations of C. taliensis. The average allele number of the microsatellites was four per locus, ranging from 2 to 7. The observed and expected heterozygosities varied from 0.076 to 0.5833 and from 0.1560 to 0.6917, respectively. Cross-species amplification in other three tea plants showed eleven of them holding promise for sister species. These polymorphic SSR markers would be useful tools for population genetics studies and assessing genetic variations to establish conservation strategy, molecular identification and molecular breeding on this tea plant and its allied species and varieties in section Thea genus Camellia.
关键词Camellia Taliensis Fiasco Microsatellite Markers Polymorphism Tea Plant
资助信息National Natural Science Foundation of China [30870169]
收录类别SCI
语种英语
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文献类型期刊论文
条目标识符http://ir.kib.ac.cn/handle/151853/11215
专题中国西南野生生物种质资源库
作者单位1.Chinese Acad Sci, Kunming Inst Bot, Key Lab Biodivers & Biogeog, Kunming 650204, Yunnan, Peoples R China
2.Jiangxi Agr Univ, Nanchang, Jiangxi, Peoples R China
3.Chinese Acad Sci, Kunming Inst Bot, SW China Germplasm Bank Wild Species, Kunming 650204, Yunnan, Peoples R China
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Yang, Jun-Bo,Yang, Jing,Li, Hong-Tao,et al. Isolation and characterization of 15 microsatellite markers from wild tea plant (Camellia taliensis) using FIASCO method[J]. CONSERVATION GENETICS,2009,10(5):1621-1623.
APA Yang, Jun-Bo,Yang, Jing,Li, Hong-Tao,Zhao, Yu,&Yang, Shi-Xiong.(2009).Isolation and characterization of 15 microsatellite markers from wild tea plant (Camellia taliensis) using FIASCO method.CONSERVATION GENETICS,10(5),1621-1623.
MLA Yang, Jun-Bo,et al."Isolation and characterization of 15 microsatellite markers from wild tea plant (Camellia taliensis) using FIASCO method".CONSERVATION GENETICS 10.5(2009):1621-1623.
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