Isolation and ectopic expression of a bamboo MADS-box gene

doi:10.1360/982004-304

	Isolation and ectopic expression of a bamboo MADS-box gene
	Tian, B ; Chen, YY ; Yan, YX ; Li, DZ
	2005-02-01
发表期刊	CHINESE SCIENCE BULLETIN
卷号	50 期号:3 页码:217-224
摘要	A cDNA named DIMADS18 was isolated from the young spikelets of the sweet bamboo, Dendrocalamus latiflorus by RACE. DNA sequence analysis showed that DIMADS18 was composed of full ORF and 3'UTR, but without 5'UTR. The cDNA contained 1039 nucleotides and encoded a putative protein of 249 amino acid residues. The gene displayed the structure of a typical plant MADS box gene, which consisted of an MADS domain, K domain, a short I region, and the C-terminal region. Phylogenetic analysis of plant MADS box genes based on amino acid sequences revealed that DIMADS18 was grouped into the AGAMOUS-LIKE 6 (AGL6)-like subfamily. It was most likely homologous to the OsMADS6 of rice (Oryza sativa), with 88% sequence identity for the entire amino acid sequences. The DIMADS18 also showed relatively high amino acid sequence identity (59%) to AGL6 of Arabidopsis thaliana. To study the functions of DWADS18, DWADS18 cDNA clone driven by the CaMV 35S promoter was transformed into Arabidopsis plants. Transgenic plants of DIMADS18 exhibited the phenotypes of curled leaves, dwarfism, and early flowering with clustered terminal flowers. These results indicated that DIMADS18 may probably be involved in controlling the flowering time of D. latiflorus.
关键词	Dendrocalamus Latiflorus Mads-box Gene Agl6-like Transgenic Plants
学科领域	Multidisciplinary Sciences
DOI	10.1360/982004-304
收录类别	SCI
语种	英语
WOS记录号	WOS:000228412100005
引用统计
文献类型	期刊论文
条目标识符	http://ir.kib.ac.cn/handle/151853/11213
专题	中国西南野生生物种质资源库
作者单位	1.Chinese Acad Sci, Lsab Plant Biodivers & Biogeog, Kunming Inst Bot, Kunming 650204, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China 3.Dalian Univ Technol, Coll Bioengn, Dalian 116622, Peoples R China
推荐引用方式 GB/T 7714	Tian, B,Chen, YY,Yan, YX,et al. Isolation and ectopic expression of a bamboo MADS-box gene[J]. CHINESE SCIENCE BULLETIN,2005,50(3):217-224.
APA	Tian, B,Chen, YY,Yan, YX,&Li, DZ.(2005).Isolation and ectopic expression of a bamboo MADS-box gene.CHINESE SCIENCE BULLETIN,50(3),217-224.
MLA	Tian, B,et al."Isolation and ectopic expression of a bamboo MADS-box gene".CHINESE SCIENCE BULLETIN 50.3(2005):217-224.

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