中国科学院昆明植物研究所机构知识库
Advanced  
KIB OpenIR  > 中国西南野生生物种质资源库  > 期刊论文
题名: Isolation and ectopic expression of a bamboo MADS-box gene
作者: Tian, B; Chen, YY; Yan, YX; null(李德铢)
刊名: CHINESE SCIENCE BULLETIN
关键词: Dendrocalamus latiflorus ; MADS-box gene ; AGL6-like ; transgenic plants
英文摘要: A cDNA named DIMADS18 was isolated from the young spikelets of the sweet bamboo, Dendrocalamus latiflorus by RACE. DNA sequence analysis showed that DIMADS18 was composed of full ORF and 3'UTR, but without 5'UTR. The cDNA contained 1039 nucleotides and encoded a putative protein of 249 amino acid residues. The gene displayed the structure of a typical plant MADS box gene, which consisted of an MADS domain, K domain, a short I region, and the C-terminal region. Phylogenetic analysis of plant MADS box genes based on amino acid sequences revealed that DIMADS18 was grouped into the AGAMOUS-LIKE 6 (AGL6)-like subfamily. It was most likely homologous to the OsMADS6 of rice (Oryza sativa), with 88% sequence identity for the entire amino acid sequences. The DIMADS18 also showed relatively high amino acid sequence identity (59%) to AGL6 of Arabidopsis thaliana. To study the functions of DWADS18, DWADS18 cDNA clone driven by the CaMV 35S promoter was transformed into Arabidopsis plants. Transgenic plants of DIMADS18 exhibited the phenotypes of curled leaves, dwarfism, and early flowering with clustered terminal flowers. These results indicated that DIMADS18 may probably be involved in controlling the flowering time of D. latiflorus.
出版日期: 2005-02-01
卷号: 50, 期号:3, 页码:217-224
DOI标识: 10.1360/982004-304
语种: 英语
收录类别: SCI
学科分类: Multidisciplinary Sciences
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.kib.ac.cn/handle/151853/11213
Appears in Collections:中国西南野生生物种质资源库_期刊论文

Files in This Item: Download All
File Name/ File Size Content Type Version Access License
201109070032.PDF(1018KB)----开放获取--View Download

作者单位: 1.Chinese Acad Sci, Lsab Plant Biodivers & Biogeog, Kunming Inst Bot, Kunming 650204, Peoples R China
2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China
3.Dalian Univ Technol, Coll Bioengn, Dalian 116622, Peoples R China

Recommended Citation:
Tian, B; Chen, YY; Yan, YX; Li, DZ.Isolation and ectopic expression of a bamboo MADS-box gene,CHINESE SCIENCE BULLETIN,2005,50(3):217-224
Service
Recommend this item
Sava as my favorate item
Show this item's statistics
Export Endnote File
Google Scholar
Similar articles in Google Scholar
[Tian, B]'s Articles
[Chen, YY]'s Articles
[Yan, YX]'s Articles
CSDL cross search
Similar articles in CSDL Cross Search
[Tian, B]‘s Articles
[Chen, YY]‘s Articles
[Yan, YX]‘s Articles
Related Copyright Policies
Null
Social Bookmarking
Add to CiteULike Add to Connotea Add to Del.icio.us Add to Digg Add to Reddit
文件名: 201109070032.PDF
格式: Adobe PDF
此文件暂不支持浏览
所有评论 (0)
暂无评论
 
评注功能仅针对注册用户开放,请您登录
您对该条目有什么异议,请填写以下表单,管理员会尽快联系您。
内 容:
Email:  *
单位:
验证码:   刷新
您在IR的使用过程中有什么好的想法或者建议可以反馈给我们。
标 题:
 *
内 容:
Email:  *
验证码:   刷新

Items in IR are protected by copyright, with all rights reserved, unless otherwise indicated.

 

 

Valid XHTML 1.0!
Copyright © 2007-2017  中国科学院昆明植物研究所 - Feedback
Powered by CSpace