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题名: Functional conservation of the plant EMBRYONIC FLOWER2 gene between bamboo and Arabidopsis
作者: Xu, Hong1, 2, 3; Chen, Ling-Jing4; Qu, Li-Jia5; Gu, Hong-Ya5; null(李德铢)1, 2
刊名: BIOTECHNOLOGY LETTERS
关键词: Anti-sense ; Bamboo EMF2 ; Rapid amplification of cDNA ends ; Real-time PCR ; Rescue
英文摘要: A novel EMBRYONGIC FLOWER2 gene, DlEMF2, was isolated from bamboo, Dendrocalamus latiflorus. It is 2518 bp in length encoding a protein of 629 amino acids. It was expressed in all organs and at a higher level in shoot tissue than in inflorescences. Transgenic Arabidopsis plants expressing antisense DlEMF2 displayed a spectrum of early-flowering phenotypes. Quantitative real-time PCR experiments indicated that the antisense transgene DlEMF2 reduced endogenous AtEMF2 transcript levels leading to the flowering time reduction. Besides, transforming the bamboo DlEMF2 gene into Arabidopsis emf2-1 mutant could rescue phenotype in vegetative and reproductive developmental stages, which reveals that EMF2 has functional conservation between monocots and eudicots.
出版日期: 2010-12-01
卷号: 32, 期号:12, 页码:1961-1968
DOI标识: 10.1007/s10529-010-0362-1
语种: 英语
收录类别: SCI
学科分类: Biotechnology & Applied Microbiology
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.kib.ac.cn/handle/151853/11195
Appears in Collections:中国西南野生生物种质资源库_期刊论文

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作者单位: 1.Chinese Acad Sci, Key Lab Biodivers & Biogeog, Kunming Inst Bot, Kunming 650204, Yunnan, Peoples R China
2.Germplasm Bank Wild Species, Plant Germplasm & Genom Ctr, Kunming 650204, Yunnan, Peoples R China
3.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China
4.Univ Calif San Francisco, Dept Hematol Oncol, San Francisco, CA 94143 USA
5.Peking Univ, Peking Yale Joint Ctr Plant Mol Genet & Agrobiote, Natl Lab Prot Engn & Plant Genet Engn, Coll Life Sci, Beijing 100871, Peoples R China

Recommended Citation:
Xu, H; Chen, LJ; Qu, LJ; Gu, HY; Li, DZ.Functional conservation of the plant EMBRYONIC FLOWER2 gene between bamboo and Arabidopsis,BIOTECHNOLOGY LETTERS,2010,32(12):1961-1968
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