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Functional conservation of the plant EMBRYONIC FLOWER2 gene between bamboo and Arabidopsis
Xu, Hong1,2,3; Chen, Ling-Jing4; Qu, Li-Jia5; Gu, Hong-Ya5; Li, De-Zhu1,2
2010-12-01
发表期刊BIOTECHNOLOGY LETTERS
卷号32期号:12页码:1961-1968
摘要A novel EMBRYONGIC FLOWER2 gene, DlEMF2, was isolated from bamboo, Dendrocalamus latiflorus. It is 2518 bp in length encoding a protein of 629 amino acids. It was expressed in all organs and at a higher level in shoot tissue than in inflorescences. Transgenic Arabidopsis plants expressing antisense DlEMF2 displayed a spectrum of early-flowering phenotypes. Quantitative real-time PCR experiments indicated that the antisense transgene DlEMF2 reduced endogenous AtEMF2 transcript levels leading to the flowering time reduction. Besides, transforming the bamboo DlEMF2 gene into Arabidopsis emf2-1 mutant could rescue phenotype in vegetative and reproductive developmental stages, which reveals that EMF2 has functional conservation between monocots and eudicots.
关键词Anti-sense Bamboo Emf2 Rapid Amplification Of Cdna Ends Real-time Pcr Rescue
资助信息Chinese Academy of Sciences [KSCX2-YW-N-029]; National Basic Research Program of China (973 Program) [2007CB411600]; National Natural Science Foundation of China [30770154]
学科领域Biotechnology & Applied Microbiology
DOI10.1007/s10529-010-0362-1
收录类别SCI
语种英语
WOS研究方向Biotechnology & Applied Microbiology
WOS类目Biotechnology & Applied Microbiology
WOS记录号WOS:000285912900029
引用统计
被引频次:8[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.kib.ac.cn/handle/151853/11195
专题中国西南野生生物种质资源库
作者单位1.Chinese Acad Sci, Key Lab Biodivers & Biogeog, Kunming Inst Bot, Kunming 650204, Yunnan, Peoples R China
2.Germplasm Bank Wild Species, Plant Germplasm & Genom Ctr, Kunming 650204, Yunnan, Peoples R China
3.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China
4.Univ Calif San Francisco, Dept Hematol Oncol, San Francisco, CA 94143 USA
5.Peking Univ, Peking Yale Joint Ctr Plant Mol Genet & Agrobiote, Natl Lab Prot Engn & Plant Genet Engn, Coll Life Sci, Beijing 100871, Peoples R China
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Xu, Hong,Chen, Ling-Jing,Qu, Li-Jia,et al. Functional conservation of the plant EMBRYONIC FLOWER2 gene between bamboo and Arabidopsis[J]. BIOTECHNOLOGY LETTERS,2010,32(12):1961-1968.
APA Xu, Hong,Chen, Ling-Jing,Qu, Li-Jia,Gu, Hong-Ya,&Li, De-Zhu.(2010).Functional conservation of the plant EMBRYONIC FLOWER2 gene between bamboo and Arabidopsis.BIOTECHNOLOGY LETTERS,32(12),1961-1968.
MLA Xu, Hong,et al."Functional conservation of the plant EMBRYONIC FLOWER2 gene between bamboo and Arabidopsis".BIOTECHNOLOGY LETTERS 32.12(2010):1961-1968.
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