DNA barcoding for the discrimination of Eurasian yews (Taxus L., Taxaceae) and the discovery of cryptic species

doi:10.1111/j.1755-0998.2010.02907.x

	DNA barcoding for the discrimination of Eurasian yews (Taxus L., Taxaceae) and the discovery of cryptic species
	Liu, Jie1,2,3 ; Moeller, Michael 4; Gao, Lian-Ming1 ; Zhang, De-Quan 1,2,3; Li, De-Zhu1,2
	2011
发表期刊	MOLECULAR ECOLOGY RESOURCES
卷号	11 期号:1 页码:89-100
摘要	There is currently international interest in the application of DNA barcoding as a tool for plant species discrimination and identification. In this study, we evaluated the utility of five candidate plant DNA barcoding regions [rbcL, matK, trnH-psbA, trnL-F and internal transcribed spacer (ITS)] in Eurasian yews. This group of species is taxonomically difficult because of a lack of clear-cut morphologically differences between species and hence represents a good test case for DNA barcoding. Forty-seven accessions were analysed, representing all taxa treated in current floristic works and covering most of the distribution range of Taxus in Eurasia. As single loci, trnL-F and ITS showed the highest species discriminatory power, each resolving 11 of 11 lineages (= barcode taxa). Species discrimination using matK, trnH-psbA and rbcL individually was lower, with matK resolving 8 of 10, trnH-psbA 7 of 11 and rbcL 5 of 11 successfully sequenced lineages. The proposed CBOL core barcode (rbcL + matK) resolved 8 of 11 lineages. Combining loci generally increased the robustness (measured by clade support) of the barcoding discrimination. Based on overall performance, trnL-F and ITS, separately or combined, are proposed as barcode for Eurasian Taxus. DNA barcoding discriminated recognized taxa of Eurasian Taxus, namely T. baccata, T. cuspidata, T. fuana and T. sumatrana, and identified seven lineages among the T. wallichiana group, some with distinct geographical distributions and morphologies, and potentially representing new species. Using the proposed DNA barcode, a technical system can be established to rapidly and reliably identify Taxus species in Eurasia for conservation protection and for monitoring illegal trade.
关键词	Conservation Cryptic Species Dna Barcode Taxonomy Taxus
学科领域	Biochemistry & Molecular Biology ; Ecology ; Evolutionary Biology
DOI	10.1111/j.1755-0998.2010.02907.x
收录类别	SCI
语种	英语
WOS记录号	WOS:000285249800010
引用统计
文献类型	期刊论文
条目标识符	http://ir.kib.ac.cn/handle/151853/11143
专题	中国西南野生生物种质资源库
作者单位	1.Chinese Acad Sci, Key Lab Biodivers & Biogeog, Kunming Inst Bot, Kunming 650204, Peoples R China 2.Chinese Acad Sci, Plant Germplasm & Genom Ctr, Germplasm Bank Wild Species, Kunming Inst Bot, Kunming 650204, Yunnan, Peoples R China 3.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China 4.Royal Bot Garden Edinburgh, Edinburgh EH3 5LR, Midlothian, Scotland
推荐引用方式 GB/T 7714	Liu, Jie,Moeller, Michael,Gao, Lian-Ming,et al. DNA barcoding for the discrimination of Eurasian yews (Taxus L., Taxaceae) and the discovery of cryptic species[J]. MOLECULAR ECOLOGY RESOURCES,2011,11(1):89-100.
APA	Liu, Jie,Moeller, Michael,Gao, Lian-Ming,Zhang, De-Quan,&Li, De-Zhu.(2011).DNA barcoding for the discrimination of Eurasian yews (Taxus L., Taxaceae) and the discovery of cryptic species.MOLECULAR ECOLOGY RESOURCES,11(1),89-100.
MLA	Liu, Jie,et al."DNA barcoding for the discrimination of Eurasian yews (Taxus L., Taxaceae) and the discovery of cryptic species".MOLECULAR ECOLOGY RESOURCES 11.1(2011):89-100.

条目包含的文件		下载所有文件
文件名称/大小	文献类型	版本类型	开放类型	使用许可
201111280001.PDF（268KB）			开放获取	--	浏览下载