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DNA barcoding for the discrimination of Eurasian yews (Taxus L., Taxaceae) and the discovery of cryptic species
Liu, Jie1,2,3; Moeller, Michael4; Gao, Lian-Ming1; Zhang, De-Quan1,2,3; Li, De-Zhu1,2
2011
发表期刊MOLECULAR ECOLOGY RESOURCES
卷号11期号:1页码:89-100
摘要There is currently international interest in the application of DNA barcoding as a tool for plant species discrimination and identification. In this study, we evaluated the utility of five candidate plant DNA barcoding regions [rbcL, matK, trnH-psbA, trnL-F and internal transcribed spacer (ITS)] in Eurasian yews. This group of species is taxonomically difficult because of a lack of clear-cut morphologically differences between species and hence represents a good test case for DNA barcoding. Forty-seven accessions were analysed, representing all taxa treated in current floristic works and covering most of the distribution range of Taxus in Eurasia. As single loci, trnL-F and ITS showed the highest species discriminatory power, each resolving 11 of 11 lineages (= barcode taxa). Species discrimination using matK, trnH-psbA and rbcL individually was lower, with matK resolving 8 of 10, trnH-psbA 7 of 11 and rbcL 5 of 11 successfully sequenced lineages. The proposed CBOL core barcode (rbcL + matK) resolved 8 of 11 lineages. Combining loci generally increased the robustness (measured by clade support) of the barcoding discrimination. Based on overall performance, trnL-F and ITS, separately or combined, are proposed as barcode for Eurasian Taxus. DNA barcoding discriminated recognized taxa of Eurasian Taxus, namely T. baccata, T. cuspidata, T. fuana and T. sumatrana, and identified seven lineages among the T. wallichiana group, some with distinct geographical distributions and morphologies, and potentially representing new species. Using the proposed DNA barcode, a technical system can be established to rapidly and reliably identify Taxus species in Eurasia for conservation protection and for monitoring illegal trade.
关键词Conservation Cryptic Species Dna Barcode Taxonomy Taxus
资助信息National Natural Science Foundation of China (NSFC) [30700042]; CAS [9223111W1, KSCX2-YW-N-0807, 540806311211]; Natural Science Foundation of Yunnan [2007C088M]; talent project of Yunnan province [2008YP064]
学科领域Biochemistry & Molecular Biology ; Ecology ; Evolutionary Biology
DOI10.1111/j.1755-0998.2010.02907.x
收录类别SCI
语种英语
WOS研究方向Biochemistry & Molecular Biology ; Environmental Sciences & Ecology ; Evolutionary Biology
WOS类目Biochemistry & Molecular Biology ; Ecology ; Evolutionary Biology
WOS记录号WOS:000285249800010
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文献类型期刊论文
条目标识符http://ir.kib.ac.cn/handle/151853/11143
专题中国西南野生生物种质资源库
作者单位1.Chinese Acad Sci, Key Lab Biodivers & Biogeog, Kunming Inst Bot, Kunming 650204, Peoples R China
2.Chinese Acad Sci, Plant Germplasm & Genom Ctr, Germplasm Bank Wild Species, Kunming Inst Bot, Kunming 650204, Yunnan, Peoples R China
3.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China
4.Royal Bot Garden Edinburgh, Edinburgh EH3 5LR, Midlothian, Scotland
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GB/T 7714
Liu, Jie,Moeller, Michael,Gao, Lian-Ming,et al. DNA barcoding for the discrimination of Eurasian yews (Taxus L., Taxaceae) and the discovery of cryptic species[J]. MOLECULAR ECOLOGY RESOURCES,2011,11(1):89-100.
APA Liu, Jie,Moeller, Michael,Gao, Lian-Ming,Zhang, De-Quan,&Li, De-Zhu.(2011).DNA barcoding for the discrimination of Eurasian yews (Taxus L., Taxaceae) and the discovery of cryptic species.MOLECULAR ECOLOGY RESOURCES,11(1),89-100.
MLA Liu, Jie,et al."DNA barcoding for the discrimination of Eurasian yews (Taxus L., Taxaceae) and the discovery of cryptic species".MOLECULAR ECOLOGY RESOURCES 11.1(2011):89-100.
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