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Applying plant DNA barcodes to identify species of Parnassia (Parnassiaceae)
Yang, Jun-Bo1,2; Wang, Yi-Ping3; Moeller, Michael4; Gao, Lian-Ming1,2; Wu, Ding3,5
2012-03-01
发表期刊MOLECULAR ECOLOGY RESOURCES
ISSN1755-098X
卷号12期号:2页码:267-275
摘要DNA barcoding is a technique to identify species by using standardized DNA sequences. In this study, a total of 105 samples, representing 30 Parnassia species, were collected to test the effectiveness of four proposed DNA barcodes (rbcL, matK, trnH-psbA and ITS) for species identification. Our results demonstrated that all four candidate DNA markers have a maximum level of primer universality and sequencing success. As a single DNA marker, the ITS region provided the highest species resolution with 86.7%, followed by trnH-psbA with 73.3%. The combination of the core barcode regions, matK+rbcL, gave the lowest species identification success (63.3%) among any combination of multiple markers and was found unsuitable as DNA barcode for Parnassia. The combination of ITS+trnH-psbA achieved the highest species discrimination with 90.0% resolution (27 of 30 sampled species), equal to the four-marker combination and higher than any two or three marker combination including rbcL or matK. Therefore, matK and rbcL should not be used as DNA barcodes for the species identification of Parnassia. Based on the overall performance, the combination of ITS+trnH-psbA is proposed as the most suitable DNA barcode for identifying Parnassia species. DNA barcoding is a useful technique and provides a reliable and effective mean for the discrimination of Parnassia species, and in combination with morphology-based taxonomy, will be a robust approach for tackling taxonomically complex groups. In the light of our findings, we found among the three species not identified a possible cryptic speciation event in Parnassia.
关键词Cryptic Species Dna Barcoding Parnassia Species Identification Taxonomy
资助信息Chinese Academy of Sciences[2009-LSF-GBOWS-01, KSCX2-YW-N-0807, KSCX2-EW-Z-2]; National Science Foundation of China[30670144]; talent project of the Yunnan province[2008YP064]; Rural and Environment Science and Analytical Services division (RESAS) in the Scottish Government
学科领域Biochemistry & Molecular Biology ; Ecology ; Evolutionary Biology
DOI10.1111/j.1755-0998.2011.03095.x
收录类别SCI
语种英语
WOS研究方向Biochemistry & Molecular Biology ; Environmental Sciences & Ecology ; Evolutionary Biology
WOS类目Biochemistry & Molecular Biology ; Ecology ; Evolutionary Biology
WOS记录号WOS:000299930300009
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被引频次:32[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.kib.ac.cn/handle/151853/10545
专题中国西南野生生物种质资源库
作者单位1.Chinese Acad Sci, Kunming Inst Bot, Key Lab Biodivers & Biogeog, Kunming 650204, Yunnan, Peoples R China
2.Chinese Acad Sci, Kunming Inst Bot, Germplasm Bank Wild Species SWChina, Kunming 650204, Yunnan, Peoples R China
3.Jiangxi Agr Univ, Nanchang 330045, Peoples R China
4.Royal Bot Garden Edinburgh, Edinburgh EH3 5LR, Midlothian, Scotland
5.Jingdezhen Coll, Jingdezhen 333000, Peoples R China
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Yang, Jun-Bo,Wang, Yi-Ping,Moeller, Michael,et al. Applying plant DNA barcodes to identify species of Parnassia (Parnassiaceae)[J]. MOLECULAR ECOLOGY RESOURCES,2012,12(2):267-275.
APA Yang, Jun-Bo,Wang, Yi-Ping,Moeller, Michael,Gao, Lian-Ming,&Wu, Ding.(2012).Applying plant DNA barcodes to identify species of Parnassia (Parnassiaceae).MOLECULAR ECOLOGY RESOURCES,12(2),267-275.
MLA Yang, Jun-Bo,et al."Applying plant DNA barcodes to identify species of Parnassia (Parnassiaceae)".MOLECULAR ECOLOGY RESOURCES 12.2(2012):267-275.
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