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题名: Applying plant DNA barcodes to identify species of Parnassia (Parnassiaceae)
作者: Yang, Jun-Bo1, 2; Wang, Yi-Ping3; Moeller, Michael4; null(高连明)1, 2; Wu, Ding3, 5
刊名: MOLECULAR ECOLOGY RESOURCES
关键词: cryptic species ; DNA barcoding ; Parnassia ; species identification ; taxonomy
英文摘要: DNA barcoding is a technique to identify species by using standardized DNA sequences. In this study, a total of 105 samples, representing 30 Parnassia species, were collected to test the effectiveness of four proposed DNA barcodes (rbcL, matK, trnH-psbA and ITS) for species identification. Our results demonstrated that all four candidate DNA markers have a maximum level of primer universality and sequencing success. As a single DNA marker, the ITS region provided the highest species resolution with 86.7%, followed by trnH-psbA with 73.3%. The combination of the core barcode regions, matK+rbcL, gave the lowest species identification success (63.3%) among any combination of multiple markers and was found unsuitable as DNA barcode for Parnassia. The combination of ITS+trnH-psbA achieved the highest species discrimination with 90.0% resolution (27 of 30 sampled species), equal to the four-marker combination and higher than any two or three marker combination including rbcL or matK. Therefore, matK and rbcL should not be used as DNA barcodes for the species identification of Parnassia. Based on the overall performance, the combination of ITS+trnH-psbA is proposed as the most suitable DNA barcode for identifying Parnassia species. DNA barcoding is a useful technique and provides a reliable and effective mean for the discrimination of Parnassia species, and in combination with morphology-based taxonomy, will be a robust approach for tackling taxonomically complex groups. In the light of our findings, we found among the three species not identified a possible cryptic speciation event in Parnassia.
出版日期: 2012-03-01
卷号: 12, 期号:2, 页码:267-275
DOI标识: 10.1111/j.1755-0998.2011.03095.x
语种: 英语
收录类别: SCI
学科分类: Biochemistry & Molecular Biology; Ecology; Evolutionary Biology
ISSN号: 1755-098X
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内容类型: 期刊论文
URI标识: http://ir.kib.ac.cn/handle/151853/10545
Appears in Collections:中国西南野生生物种质资源库_期刊论文

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作者单位: 1.Chinese Acad Sci, Kunming Inst Bot, Key Lab Biodivers & Biogeog, Kunming 650204, Yunnan, Peoples R China
2.Chinese Acad Sci, Kunming Inst Bot, Germplasm Bank Wild Species SWChina, Kunming 650204, Yunnan, Peoples R China
3.Jiangxi Agr Univ, Nanchang 330045, Peoples R China
4.Royal Bot Garden Edinburgh, Edinburgh EH3 5LR, Midlothian, Scotland
5.Jingdezhen Coll, Jingdezhen 333000, Peoples R China

Recommended Citation:
Yang, JB; Wang, YP; Moller, M; Gao, LM; Wu, D.Applying plant DNA barcodes to identify species of Parnassia (Parnassiaceae),MOLECULAR ECOLOGY RESOURCES,2012,12(2):267-275
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