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Development of 16 Microsatellite Markers for Prince's Pine, Chimaphila japonica (Pyroleae, Monotropoideae, Ericaceae)
Liu, Zhenwen2; Zhao, Qianru2,3; Zhou, Jing4,5; Peng, Hua2; Yang, Junbo1
2012-04-01
发表期刊INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
ISSN1661-6596
卷号13期号:4页码:4003-4008
摘要The perennial evergreen herb, Chimaphila japonica is found exclusively in East Asian temperate coniferous or sometimes in deciduous forests. By using the Fast Isolation by Amplified Fragment Length Polymorphism (AFLP) of Sequences Containing repeats (FIASCO) protocol, 20 microsatellite primer sets were identified in two wild populations. Of these primers, 16 displayed polymorphisms and 4 were monomorphic. The number of alleles per locus ranged from one to six among populations, values for expected and observed heterozygosities ranged from 0.000 to 0.848 and from 0.000 to 1.000, respectively. The new SSR markers will be useful in obtaining estimates of population-level genetic diversity and in phylogeographic studies of C. japonica.
关键词Chimaphila Japonica Microsatellite Marker Polymorphism Population Genetics
资助信息National Natural Science Foundation of China[30900075, 31000098]; State Key Laboratory of Systematic and Evolutionary Botany (LSEB)
学科领域Chemistry, Multidisciplinary
DOI10.3390/ijms13044003
收录类别SCI
语种英语
WOS研究方向Chemistry
WOS类目Chemistry, Multidisciplinary
WOS记录号WOS:000303454700001
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被引频次:1[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.kib.ac.cn/handle/151853/10535
专题中国西南野生生物种质资源库
作者单位1.Chinese Acad Sci, Kunming Inst Bot, Germplasm Bank Wild Species SW China, Kunming 650201, Yunnan, Peoples R China
2.Chinese Acad Sci, Kunming Inst Bot, Key Lab Biodivers & Biogeog, Kunming 650201, Peoples R China
3.Chinese Acad Sci, Grad Sch, Beijing 100049, Peoples R China
4.Kunming Med Univ, Sch Pharmaceut Sci, Kunming 650500, Yunnan, Peoples R China
5.Kunming Med Univ, Yunnan Key Lab Pharmacol Nat Prod, Kunming 650500, Yunnan, Peoples R China
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Liu, Zhenwen,Zhao, Qianru,Zhou, Jing,et al. Development of 16 Microsatellite Markers for Prince's Pine, Chimaphila japonica (Pyroleae, Monotropoideae, Ericaceae)[J]. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES,2012,13(4):4003-4008.
APA Liu, Zhenwen,Zhao, Qianru,Zhou, Jing,Peng, Hua,&Yang, Junbo.(2012).Development of 16 Microsatellite Markers for Prince's Pine, Chimaphila japonica (Pyroleae, Monotropoideae, Ericaceae).INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES,13(4),4003-4008.
MLA Liu, Zhenwen,et al."Development of 16 Microsatellite Markers for Prince's Pine, Chimaphila japonica (Pyroleae, Monotropoideae, Ericaceae)".INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES 13.4(2012):4003-4008.
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