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题名: Development of 16 Microsatellite Markers for Prince's Pine, Chimaphila japonica (Pyroleae, Monotropoideae, Ericaceae)
作者: Liu, Zhenwen2; Zhao, Qianru2, 3; Zhou, Jing4, 5; null(彭华)2; Yang, Junbo1
刊名: INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
关键词: Chimaphila japonica ; microsatellite marker ; polymorphism ; population genetics
英文摘要: The perennial evergreen herb, Chimaphila japonica is found exclusively in East Asian temperate coniferous or sometimes in deciduous forests. By using the Fast Isolation by Amplified Fragment Length Polymorphism (AFLP) of Sequences Containing repeats (FIASCO) protocol, 20 microsatellite primer sets were identified in two wild populations. Of these primers, 16 displayed polymorphisms and 4 were monomorphic. The number of alleles per locus ranged from one to six among populations, values for expected and observed heterozygosities ranged from 0.000 to 0.848 and from 0.000 to 1.000, respectively. The new SSR markers will be useful in obtaining estimates of population-level genetic diversity and in phylogeographic studies of C. japonica.
出版日期: 2012-04-01
卷号: 13, 期号:4, 页码:4003-4008
DOI标识: 10.3390/ijms13044003
语种: 英语
ISSN号: 1661-6596
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.kib.ac.cn/handle/151853/10535
Appears in Collections:中国西南野生生物种质资源库_期刊论文

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作者单位: 1.Chinese Acad Sci, Kunming Inst Bot, Germplasm Bank Wild Species SW China, Kunming 650201, Yunnan, Peoples R China
2.Chinese Acad Sci, Kunming Inst Bot, Key Lab Biodivers & Biogeog, Kunming 650201, Peoples R China
3.Chinese Acad Sci, Grad Sch, Beijing 100049, Peoples R China
4.Kunming Med Univ, Sch Pharmaceut Sci, Kunming 650500, Yunnan, Peoples R China
5.Kunming Med Univ, Yunnan Key Lab Pharmacol Nat Prod, Kunming 650500, Yunnan, Peoples R China

Recommended Citation:
Liu, ZW; Zhao, QR; Zhou, J; Peng, H; Yang, JB.Development of 16 Microsatellite Markers for Prince's Pine, Chimaphila japonica (Pyroleae, Monotropoideae, Ericaceae),INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES,2012,13(4):4003-4008
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