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Isolation and Characterization of 21 Microsatellite Loci in Cardiocrinum giganteum var. yunnanense (Liliaceae), an Important Economic Plant in China
Li, Rong2; Yang, Jie3,4; Yang, Junbo1; Dao, Zhiling2
2012-02-01
发表期刊INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
ISSN1661-6596
卷号13期号:2页码:1437-1443
摘要Twenty-one microsatellite markers from the genome of Cardiocrinum giganteum var. yunnanense, an important economic plant in China, were developed with a fast isolation protocol by amplified fragment length polymorphism of sequences containing repeats (FIASCO). Polymorphism within each locus was assessed in 24 wild individuals from Gaoligong Mountains in western Yunnan Province, China. The number of alleles per locus ranged from 2 to 4 with a mean of 2.9. The expected and observed levels of heterozygosity ranged from 0.042 to 0.726 and from 0.000 to 1.000, with averages of 0.44 and 0.31, respectively. These polymorphic microsatellite markers should prove useful in population genetics studies and assessments of genetic variation to develop conservation and management strategies for this species.
关键词Cardiocrinum Giganteum Var. Yunnanense Microsatellite Markers Polymorphism Population Genetics Liliaceae
资助信息Chinese Academy of Sciences[2010KIBA06]; Natural Science Foundation of Mianyang[09S002-7]
学科领域Chemistry ; Multidisciplinary
DOI10.3390/ijms13021437
收录类别SCI
语种英语
WOS研究方向Chemistry
WOS类目Chemistry, Multidisciplinary
WOS记录号WOS:000300715700011
引用统计
被引频次:3[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.kib.ac.cn/handle/151853/10533
专题中国西南野生生物种质资源库
作者单位1.Chinese Acad Sci, Kunming Inst Bot, Germplasm Bank Wild Species SW China, Kunming 650201, Yunnan, Peoples R China
2.Chinese Acad Sci, Kunming Inst Bot, Key Lab Biodivers & Biogeog, Kunming 650201, Yunnan, Peoples R China
3.Chinese Acad Sci, Key Lab Trop Forest Ecol, Xishuangbanna Trop Bot Garden, Mengla 666303, Yunnan, Peoples R China
4.Sichuan Tradit Chinese Med Coll, Pharm & Inspect Dept, Mianyang 621000, Sichuan, Peoples R China
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GB/T 7714
Rong Li, Jie Yang, Junbo Yang, Zhiling Dao. 2012. Isolation and characterization of 21 microsatellite loci in Cardiocrinum giganteum var. yunnanense (Liliaceae), an important economic plant in China. International Journal of Molecular Sciences 13: 1437-1443.
APA Li, Rong,Yang, Jie,Yang, Junbo,&Dao, Zhiling.(2012).Isolation and Characterization of 21 Microsatellite Loci in Cardiocrinum giganteum var. yunnanense (Liliaceae), an Important Economic Plant in China.INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES,13(2),1437-1443.
MLA Li, Rong,et al."Isolation and Characterization of 21 Microsatellite Loci in Cardiocrinum giganteum var. yunnanense (Liliaceae), an Important Economic Plant in China".INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES 13.2(2012):1437-1443.
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