Isolation and Characterization of 21 Microsatellite Loci in Cardiocrinum giganteum var. yunnanense (Liliaceae), an Important Economic Plant in China | |
Li, Rong2; Yang, Jie3,4; Yang, Junbo1; Dao, Zhiling2 | |
2012-02-01 | |
发表期刊 | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES |
ISSN | 1661-6596 |
卷号 | 13期号:2页码:1437-1443 |
摘要 | Twenty-one microsatellite markers from the genome of Cardiocrinum giganteum var. yunnanense, an important economic plant in China, were developed with a fast isolation protocol by amplified fragment length polymorphism of sequences containing repeats (FIASCO). Polymorphism within each locus was assessed in 24 wild individuals from Gaoligong Mountains in western Yunnan Province, China. The number of alleles per locus ranged from 2 to 4 with a mean of 2.9. The expected and observed levels of heterozygosity ranged from 0.042 to 0.726 and from 0.000 to 1.000, with averages of 0.44 and 0.31, respectively. These polymorphic microsatellite markers should prove useful in population genetics studies and assessments of genetic variation to develop conservation and management strategies for this species. |
关键词 | Cardiocrinum Giganteum Var. Yunnanense Microsatellite Markers Polymorphism Population Genetics Liliaceae |
学科领域 | Chemistry ; Multidisciplinary |
DOI | 10.3390/ijms13021437 |
收录类别 | SCI |
语种 | 英语 |
WOS记录号 | WOS:000300715700011 |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | http://ir.kib.ac.cn/handle/151853/10533 |
专题 | 中国西南野生生物种质资源库 |
作者单位 | 1.Chinese Acad Sci, Kunming Inst Bot, Germplasm Bank Wild Species SW China, Kunming 650201, Yunnan, Peoples R China 2.Chinese Acad Sci, Kunming Inst Bot, Key Lab Biodivers & Biogeog, Kunming 650201, Yunnan, Peoples R China 3.Chinese Acad Sci, Key Lab Trop Forest Ecol, Xishuangbanna Trop Bot Garden, Mengla 666303, Yunnan, Peoples R China 4.Sichuan Tradit Chinese Med Coll, Pharm & Inspect Dept, Mianyang 621000, Sichuan, Peoples R China |
推荐引用方式 GB/T 7714 | Rong Li, Jie Yang, Junbo Yang, Zhiling Dao. 2012. Isolation and characterization of 21 microsatellite loci in Cardiocrinum giganteum var. yunnanense (Liliaceae), an important economic plant in China. International Journal of Molecular Sciences 13: 1437-1443. |
APA | Li, Rong,Yang, Jie,Yang, Junbo,&Dao, Zhiling.(2012).Isolation and Characterization of 21 Microsatellite Loci in Cardiocrinum giganteum var. yunnanense (Liliaceae), an Important Economic Plant in China.INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES,13(2),1437-1443. |
MLA | Li, Rong,et al."Isolation and Characterization of 21 Microsatellite Loci in Cardiocrinum giganteum var. yunnanense (Liliaceae), an Important Economic Plant in China".INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES 13.2(2012):1437-1443. |
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