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中国科学院昆明植物研究所知识管理系统
Knowledge Management System of Kunming Institute of Botany,CAS
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专题:昆明植物所硕博研究生毕业学位论文
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资助项目:1. Seed dormancy type ofManglietia grandis seeds, The seeds of Manglietia grandis had good water-absorption and the embryos were underdeveloped. Fresh seeds only germinated to 2% at 25 ℃, so the seeds were morphophysiological dormant. The seeds germinated to 82% at 25/15 ℃,the embryos do not need 0~10 ℃ stratification to break the morphophysiological dormancy and only grew obviously when they were nearly germinated. So we can say that the seeds of Manglietia grandis have non-deep morphophysiological simple dormancy, and the cold stratification can break the physical dormancy component of there complex dormancies. The 50 days germination rate of Manglietia grandis seeds was higher than the 30 day germination rate at 30/20℃,25/15℃,20/10℃, and the GA3 had broken the seeds physical dormancy effectively, so the Manglietia grandis seeds were conditional dormant. After different time cold stratifications, we found that the 60 days stratification was the most effective way to break the dormancy. The others’ stratifications made a long time and moist environment, so the seeds would have a secondary dormancy, entering into the dormancy circle. 2. The way to break the dormancy of Manglietia grandis seeds effectively and to improve the germination, 1000 mg·L-1 GA3 could break the dormancy of Manglietia grandis seeds at 20~30℃ somehow. 6-BA and KNO3 couldn’t improve germination effectively, but these two chemicals could make a considerably high germination at 20/10℃ no matter with the concentration. And the differences of each other were not remarkable. The dormancy of 60 days cold stratification Manglietia grandis seeds almost could been broken. But at 20/10℃ the highest germination happened at the 40 days stratification, the 100 days stratification’ s germination also high to 82%. 3. The storage behavior of Manglietia grandis seeds, After the desiccation tolerance test, we can know generally that the storage behavior of Manglietia grandis seeds are intermediate type. In the further experiment, when the seeds were dehydrated to 10~12% water content, the viability was above 90%. Go on the dehydration to 5% water content, the seeds viability was 87%,when the water content was 3.7%, there still were considerable live seeds. Then the seeds were air tightly stored at -20℃ for 3 months, the viability was only about 30%(comprehend the viability of 5% and 4.7% water content together), most seeds lost their viability. So the Manglietia grandis seeds were intermediate seeds. 4. The storage condition of Manglietia grandis seeds, The Manglietia grandis seeds can endure 4℃ low temperature, and keep the viability for quite a long time, but can’t endure the sub-zero low temperature. At the 10℃ above temperature, the seeds viability went down as the water content lost. So we can airtightly store the Manglietia grandis seeds directly at 4℃ or make proper dehydration to about 10% water content then store at -20℃. After low temperature moist storage 60 days and 120 days, the seeds viability were all above 90%, we can concluded that the low temperature moist storage was more effective than the dry storage, and easy to manipulate. 5. The in vitro culture of Manglietia grandis seeds, The in vitro culture experiment used seedlings of Manglietia grandis as the explants. Put the seedlings which only have the cotyledons into the culture medium of H+6-BA0.25+NAA0.125+KT0.1+IAA0.05 mg·L-1(the fallowing units are as the same), several adventitious buds had be induced out. Put all these adventitious buds into the medium of H+6-BA0.5+ KT0.1, the multiplication coefficient is 3.5, and all of the buds grew well. It’s not hard to induce out the adventitious buds and make proliferations, the problem was that during the process there had be browning effects on the seedlings and buds, the problem was settled by cutting and using the antioxidants