中国科学院昆明植物研究所知识管理系统(KIB OpenIR): Search Results

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Community：昆明植物所硕博研究生毕业学位论文 First author affiliation The first author Corresponding Author Funding Project：Aconitum carmichaeli Debx., a traditional Chinese medicinal herb, which has been cultivated for more than one thousand years, and has different morphological and cytological characters. Until now, the questions about its original and so on are still unknown. In this study, molecular phylogeny and phylogeography have been adopted to tracing the original plant(s) of Chuanwu, distinguished it from A. kusnezoffii and other medicinal Aconitum plants. With the help of nrDNA and cpDNA sequencing, the members of the A. carmichaeli polyploid complex were reclassified, and the relationships between the different clusters were also studied. Finally, based on these data, phylogeographic studies were carried out for the complex and Chuanwu, the origin of theChuanwu and the formation of the authentic characters were proposed. Main conclusions are summarized as follows: 1. Aconitum carmichaeli var. carmichaeli and A. carmichaeli var. pubescens were most likely the botanical original plants of Chuanwu, and the former was the principal source. 2. A 56 bp sequence in the psbA-trnH intergenic spacer could distinguish the A. carmichaeli and A. kusnezoffii from the other Aconitum medicinal species. 3. Aconitum carmichaeli polyploid complex includes the following taxa: A. carmichaeli, A. kusnezoffii, A. jaluense and A. paniculigerum var. wulingense. A. carmichaeli var. carmichaeli probablycame from the hybridization between A. carmichaeli var. hwangshanicum and A. carmichaeli var. tripartitum, and A. carmichaeli var. pubescens is likely ahybridized product of Northeastern region taxa (A. kusnezoffii, A. paniculigerum var. wulingense) and Central region taxon (A. carmichaeli var. hwangshanicum), while A. carmichaeli var. hwangshanicum, A. carmichaeli var. tripartitum and A. paniculigerum var. wulingense might all contribute to the speciation of A. carmichaeli var. truppelianum. 4. Based on ndhA and psbA-trnH intergenic spacer sequence data in 45 populations of 525 individuals of A. carmichaeli polyploid complex 32 haplotypes were revealed. High genetic diversity at the complex level and relatively low genetic diversity at the population level. Some refugia may be located in Mts. Dabieshan (JZ), Huangshan (HS) and Changbaishan (QS), and these populations may all expand or immigrate to southward or（and）northward via Mts. Taihangshan and Dabieshan-Tongbaishan during the intreglacial/glacial cycles. Mts. Qinling and Nanling have important effects on the haplotype’s pattern of A. carmichaeli var. carmichaeli. 5. The results indicated that the Chuanwu had very low genetic diversity and genetic differentiation. Chuanwu could have originated from at least two places twice. Range expansion of the original plants was likely the main genetic differentiation model for the formation of genuine character of Chuanwu.

中国科学院昆明植物研究所知识管理系统

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