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中国科学院昆明植物研究所知识管理系统
Knowledge Management System of Kunming Institute of Botany,CAS
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昆明植物所硕博研究... [46]
中国科学院东亚植物... [20]
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中国西南野生生物种质... [4]
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龚洵 [5]
Sun Hang [5]
许建初 [5]
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0.05) between wild (AR = 4.651), semi-cultivated (AR = 5.091) and cultivated (AR = 5.132) populations of C. taliensis, which suggested that the genetic background of long-lived woody plant was not easy to be changed, and there were moderate high gene flow between populations. However, there was a significant difference (P < 0.05) between wild (AR = 5.9) and cultivated (AR = 7.1) populations distributed in the same place in Yun county, Yunnan province, which may result from the hybridization and introgression of species in the tea garden and anthropogenic damages to the wild population. The hypothesis of hybrid origin of C. grandibracteata was tested by morphological and microsatellites analyses. Compared with other species, the locules in ovary of C. grandibracteata are variable, which showed a morphological intermediate and mosaic. Except one private allele, Ninety-nine percent alleles of C. grandibracteata were shared with these of C. taliensis and C. sinensis var. assamica. And C. grandibracteata was nested in the cluster of C. taliensis in the UPGMA tree. Conclusively, our results supported the hypothesis of hybrid origin of C. grandibracteata partly. The speciation of C. grandibracteata was derived from hybridization and asymmetrical introgression potentially. It is possible that C. taliensis was one of its parents, but it still needs more evidences to prove that C. sinensis var. assamica was another 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Academy of Sciences[2013T2S0030]","jscount":"1","jsurl":"/simple-search?field1=all&rpp=10&accurate=false&advanced=false&sort_by=2&isNonaffiliated=false&search_type=-1&query1=Helianthus%2Bannuus%2BL&order=desc&&fq=dc.project.title_filter%3AChinese%5C+Academy%5C+of%5C+Sciences%5C%5B2013T2S0030%5C%5D"},{"jsname":"National Natural Science Foundation of China[31470336]","jscount":"1","jsurl":"/simple-search?field1=all&rpp=10&accurate=false&advanced=false&sort_by=2&isNonaffiliated=false&search_type=-1&query1=Helianthus%2Bannuus%2BL&order=desc&&fq=dc.project.title_filter%3ANational%5C+Natural%5C+Science%5C+Foundation%5C+of%5C+China%5C%5B31470336%5C%5D"},{"jsname":"National Natural Science Foundation of China[31600178]","jscount":"1","jsurl":"/simple-search?field1=all&rpp=10&accurate=false&advanced=false&sort_by=2&isNonaffiliated=false&search_type=-1&query1=Helianthus%2Bannuus%2BL&order=desc&&fq=dc.project.title_filter%3ANational%5C+Natural%5C+Science%5C+Foundation%5C+of%5C+China%5C%5B31600178%5C%5D"},{"jsname":"National Research Council of Thailand (Mae Fah Luang University)[592010200112]","jscount":"1","jsurl":"/simple-search?field1=all&rpp=10&accurate=false&advanced=false&sort_by=2&isNonaffiliated=false&search_type=-1&query1=Helianthus%2Bannuus%2BL&order=desc&&fq=dc.project.title_filter%3ANational%5C+Research%5C+Council%5C+of%5C+Thailand%5C+%5C%28Mae%5C+Fah%5C+Luang%5C+University%5C%29%5C%5B592010200112%5C%5D"},{"jsname":"National Research Council of Thailand (Mae Fah Luang University)[60201000201]","jscount":"1","jsurl":"/simple-search?field1=all&rpp=10&accurate=false&advanced=false&sort_by=2&isNonaffiliated=false&search_type=-1&query1=Helianthus%2Bannuus%2BL&order=desc&&fq=dc.project.title_filter%3ANational%5C+Research%5C+Council%5C+of%5C+Thailand%5C+%5C%28Mae%5C+Fah%5C+Luang%5C+University%5C%29%5C%5B60201000201%5C%5D"},{"jsname":"National Science Foundation of China (NSFC)[31750110478]","jscount":"1","jsurl":"/simple-search?field1=all&rpp=10&accurate=false&advanced=false&sort_by=2&isNonaffiliated=false&search_type=-1&query1=Helianthus%2Bannuus%2BL&order=desc&&fq=dc.project.title_filter%3ANational%5C+Science%5C+Foundation%5C+of%5C+China%5C+%5C%28NSFC%5C%29%5C%5B31750110478%5C%5D"},{"jsname":"Polyploidy, the presence of three or more genomes in an organism, has occurred extensively in plants, and plays a major role in the evolution and speciation of angiosperm. Despite extensive study of the cytotypes distribution and origin of polyploidy, few studies have been reported in China, especially in southwest region. Allium wallichii Kunth (Alliaceae) is a perennial herb, distributed in southwest China, northen India, Nepal, Sikkim and Bhutan. The multiple ploidy levels and rapid differentiation has made A. wallichii a good candidate for studying polyploidy. The cytotypes distribution and origin of A. wallichii polyploidy has been studied in Yunnan-Guizhou Plateu, a main distribution area of this plant. Root-tip squashes were used to identify the ploidy level and karyotypes of 412 plants from 17 populations sampled from Yunnan-Guizhou Plateau. Based on nuclear ITS DNA sequences in 83 individuals from 17 populations, phylogentic analysis were performed to investigate types of A. wallichii polyloids, and determine if the reproductive isolation has been established between diploids and tetraploids. Based on two chloroplast DNA (cpDNA) fragments (petL-psbE, trnQ-rps16), haplotypes were identified , and the origination of tetraploids were analyzed. The main results and conclusions are as follows: 1. Distribution of cytotypes, Cytotype distribution was analyzed based on 412 newly studied plants from seventeen populations and published literatures. Nine diploid populations, six diploid-tetraploid mixed populations occur in central to northwest Yunnan, while twelve tetraploid populations occur in the Yunnan-Guizhou Plateau. Furthermore, tetraploids has a wider altitude range (1400-3726 m) than diploids (2100-3638 m), which suggests the adaptative ability of tetraploid is much stronger than its diploid pregnancies. Tetraploid populations distributed in northwest Yunnan have much lower karyotype asymmetry with the asymmetry indexes (AI) ranges from 1.83 to 2.87 compared to other populations of other areas (AI, 2.03-3.02). This suggests tetraploid in northwest Yunnan is likely to have an earlier derivation. 2. Autoploidization origin of the tetraploids, Diploids are all 2A type except that Zhongdian D and Baoshan populations are 3A type, and tetraploids are all 2A type but Huize and Hezhang populations are 2B type. It shows high similarity of karyotypes of diploid and tetraploids. In addition, the undistinguishable morphology of A. wallichii with different ploidy levels in northwest Yunnan and the monophyly of A. wallichii in ITS strict consensus tree all suggest autopolyploid origins of tetraploids A. wallichii. 3. Multiple origins of tetraploids, Based on two cpDNA fragments (petL-psbE, trnQ-rps16) in 85 individuals from 17 populations across the Yunnan-Guizhou Plateau, a total of 17 haplotypes were identified, among them, 3 in diploids only, 11 in tetraploids only, and 3 found in both cytotypes. This, plus network analyses, indicated that tetraploids have arisen independently from diploids at least three times. 4. Productive isolation between diploids and teraploids, ITS phylogenetic analyses between diploid and tetraploid A. wallichii shows that diploids and tetraploids are both monophyly, with bootstrap value 100% and 88% respectively, indicating that the reproductive isolation has been established between them. Based on cpDNA haplotypes and ITS variation types analyses, extensive hybridization and gene introgression may have occurred among tetraploids.","jscount":"1","jsurl":"/simple-search?field1=all&rpp=10&accurate=false&advanced=false&sort_by=2&isNonaffiliated=false&search_type=-1&query1=Helianthus%2Bannuus%2BL&order=desc&&fq=dc.project.title_filter%3APolyploidy%2C%5C+the%5C+presence%5C+of%5C+three%5C+or%5C+more%5C+genomes%5C+in%5C+an%5C+organism%2C%5C+has%5C+occurred%5C+extensively%5C+in%5C+plants%2C%5C+and%5C+plays%5C+a%5C+major%5C+role%5C+in%5C+the%5C+evolution%5C+and%5C+speciation%5C+of%5C+angiosperm.%5C+Despite%5C+extensive%5C+study%5C+of%5C+the%5C+cytotypes%5C+distribution%5C+and%5C+origin%5C+of%5C+polyploidy%2C%5C+few%5C+studies%5C+have%5C+been%5C+reported%5C+in%5C+China%2C%5C+especially%5C+in%5C+southwest%5C+region.%5C+Allium%5C+wallichii%5C+Kunth%5C+%5C%28Alliaceae%5C%29%5C+is%5C+a%5C+perennial%5C+herb%2C%5C+distributed%5C+in%5C+southwest%5C+China%2C%5C+northen%5C+India%2C%5C+Nepal%2C%5C+Sikkim%5C+and%5C+Bhutan.%5C+The%5C+multiple%5C+ploidy%5C+levels%5C+and%5C+rapid%5C+differentiation%5C+has%5C+made%5C+A.%5C+wallichii%5C+a%5C+good%5C+candidate%5C+for%5C+studying%5C+polyploidy.%5C+The%5C+cytotypes%5C+distribution%5C+and%5C+origin%5C+of%5C+A.%5C+wallichii%5C+polyploidy%5C+has%5C+been%5C+studied%5C+in%5C+Yunnan%5C-Guizhou%5C+Plateu%2C%5C+a%5C+main%5C+distribution%5C+area%5C+of%5C+this%5C+plant.%5C+Root%5C-tip%5C+squashes%5C+were%5C+used%5C+to%5C+identify%5C+the%5C+ploidy%5C+level%5C+and%5C+karyotypes%5C+of%5C+412%5C+plants%5C+from%5C+17%5C+populations%5C+sampled%5C+from%5C+Yunnan%5C-Guizhou%5C+Plateau.%5C+Based%5C+on%5C+nuclear%5C+ITS%5C+DNA%5C+sequences%5C+in%5C+83%5C+individuals%5C+from%5C+17%5C+populations%2C%5C+phylogentic%5C+analysis%5C+were%5C+performed%5C+to%5C+investigate%5C+types%5C+of%5C+A.%5C+wallichii%5C+polyloids%2C%5C+and%5C+determine%5C+if%5C+the%5C+reproductive%5C+isolation%5C+has%5C+been%5C+established%5C+between%5C+diploids%5C+and%5C+tetraploids.%5C+Based%5C+on%5C+two%5C+chloroplast%5C+DNA%5C+%5C%28cpDNA%5C%29%5C+fragments%5C+%5C%28petL%5C-psbE%2C%5C+trnQ%5C-rps16%5C%29%2C%5C+haplotypes%5C+were%5C+identified%5C+%2C%5C+and%5C+the%5C+origination%5C+of%5C+tetraploids%5C+were%5C+analyzed.%5C+The%5C+main%5C+results%5C+and%5C+conclusions%5C+are%5C+as%5C+follows%5C%3A%5C+1.%5C+Distribution%5C+of%5C+cytotypes%2C%5C+Cytotype%5C+distribution%5C+was%5C+analyzed%5C+based%5C+on%5C+412%5C+newly%5C+studied%5C+plants%5C+from%5C+seventeen%5C+populations%5C+and%5C+published%5C+literatures.%5C+Nine%5C+diploid%5C+populations%2C%5C+six%5C+diploid%5C-tetraploid%5C+mixed%5C+populations%5C+occur%5C+in%5C+central%5C+to%5C+northwest%5C+Yunnan%2C%5C+while%5C+twelve%5C+tetraploid%5C+populations%5C+occur%5C+in%5C+the%5C+Yunnan%5C-Guizhou%5C+Plateau.%5C+Furthermore%2C%5C+tetraploids%5C+has%5C+a%5C+wider%5C+altitude%5C+range%5C+%5C%281400%5C-3726%5C+m%5C%29%5C+than%5C+diploids%5C+%5C%282100%5C-3638%5C+m%5C%29%2C%5C+which%5C+suggests%5C+the%5C+adaptative%5C+ability%5C+of%5C+tetraploid%5C+is%5C+much%5C+stronger%5C+than%5C+its%5C+diploid%5C+pregnancies.%5C+Tetraploid%5C+populations%5C+distributed%5C+in%5C+northwest%5C+Yunnan%5C+have%5C+much%5C+lower%5C+karyotype%5C+asymmetry%5C+with%5C+the%5C+asymmetry%5C+indexes%5C+%5C%28AI%5C%29%5C+ranges%5C+from%5C+1.83%5C+to%5C+2.87%5C+compared%5C+to%5C+other%5C+populations%5C+of%5C+other%5C+areas%5C+%5C%28AI%2C%5C+2.03%5C-3.02%5C%29.%5C+This%5C+suggests%5C+tetraploid%5C+in%5C+northwest%5C+Yunnan%5C+is%5C+likely%5C+to%5C+have%5C+an%5C+earlier%5C+derivation.%5C+2.%5C+Autoploidization%5C+origin%5C+of%5C+the%5C+tetraploids%2C%5C+Diploids%5C+are%5C+all%5C+2A%5C+type%5C+except%5C+that%5C+Zhongdian%5C+D%5C+and%5C+Baoshan%5C+populations%5C+are%5C+3A%5C+type%2C%5C+and%5C+tetraploids%5C+are%5C+all%5C+2A%5C+type%5C+but%5C+Huize%5C+and%5C+Hezhang%5C+populations%5C+are%5C+2B%5C+type.%5C+It%5C+shows%5C+high%5C+similarity%5C+of%5C+karyotypes%5C+of%5C+diploid%5C+and%5C+tetraploids.%5C+In%5C+addition%2C%5C+the%5C+undistinguishable%5C+morphology%5C+of%5C+A.%5C+wallichii%5C+with%5C+different%5C+ploidy%5C+levels%5C+in%5C+northwest%5C+Yunnan%5C+and%5C+the%5C+monophyly%5C+of%5C+A.%5C+wallichii%5C+in%5C+ITS%5C+strict%5C+consensus%5C+tree%5C+all%5C+suggest%5C+autopolyploid%5C+origins%5C+of%5C+tetraploids%5C+A.%5C+wallichii.%5C+3.%5C+Multiple%5C+origins%5C+of%5C+tetraploids%2C%5C+Based%5C+on%5C+two%5C+cpDNA%5C+fragments%5C+%5C%28petL%5C-psbE%2C%5C+trnQ%5C-rps16%5C%29%5C+in%5C+85%5C+individuals%5C+from%5C+17%5C+populations%5C+across%5C+the%5C+Yunnan%5C-Guizhou%5C+Plateau%2C%5C+a%5C+total%5C+of%5C+17%5C+haplotypes%5C+were%5C+identified%2C%5C+among%5C+them%2C%5C+3%5C+in%5C+diploids%5C+only%2C%5C+11%5C+in%5C+tetraploids%5C+only%2C%5C+and%5C+3%5C+found%5C+in%5C+both%5C+cytotypes.%5C+This%2C%5C+plus%5C+network%5C+analyses%2C%5C+indicated%5C+that%5C+tetraploids%5C+have%5C+arisen%5C+independently%5C+from%5C+diploids%5C+at%5C+least%5C+three%5C+times.%5C+4.%5C+Productive%5C+isolation%5C+between%5C+diploids%5C+and%5C+teraploids%2C%5C+ITS%5C+phylogenetic%5C+analyses%5C+between%5C+diploid%5C+and%5C+tetraploid%5C+A.%5C+wallichii%5C+shows%5C+that%5C+diploids%5C+and%5C+tetraploids%5C+are%5C+both%5C+monophyly%2C%5C+with%5C+bootstrap%5C+value%5C+100%25%5C+and%5C+88%25%5C+respectively%2C%5C+indicating%5C+that%5C+the%5C+reproductive%5C+isolation%5C+has%5C+been%5C+established%5C+between%5C+them.%5C+Based%5C+on%5C+cpDNA%5C+haplotypes%5C+and%5C+ITS%5C+variation%5C+types%5C+analyses%2C%5C+extensive%5C+hybridization%5C+and%5C+gene%5C+introgression%5C+may%5C+have%5C+occurred%5C+among%5C+tetraploids."},{"jsname":"Thailand Research Fund (TRF)[RSA5980068]","jscount":"1","jsurl":"/simple-search?field1=all&rpp=10&accurate=false&advanced=false&sort_by=2&isNonaffiliated=false&search_type=-1&query1=Helianthus%2Bannuus%2BL&order=desc&&fq=dc.project.title_filter%3AThailand%5C+Research%5C+Fund%5C+%5C%28TRF%5C%29%5C%5BRSA5980068%5C%5D"},{"jsname":"Through investigating sympatric distribution of Rhododendron irroratum, examing the variation of floral characters and sequencing the ITS and other chloroplast segements, we find that (1) R. irroratum might be of hybrid origin, with its maternal parent R. delavayi or R. decorum. (2) The ancestral haplotype of R. irroratum might be identical to that of R. decorum, and it is under ongoing introgression from R. delavayi. 1. The natural distribution, Seven distribution sites of R. irroratum in Guizhou and Yunnan province were investigated. The result shows that R. irroratum is sympatric with R. delavayi, R. decorum and R. agastum. R. delavayi is widespread across the above-mentioned seven sites, whereas R. agastum is scarce in DaPingDi, HuaDianBa and HeQing sites. R. irroratum and R. agastum distribute at the higher elevation compared to that of R. decorum, while R. delavayi is of widespread distribution across both regions of R. decorum and R. irroratum.2. Floral variation of R. irroratum among populations, The floral characters remain vary within and among populations except for the stamen number and the petal number. Seven floral characters correlates with each other among populations, of 28 different combinations, 26 reveal significant correlation, and 23 extremely significant correlation. The PCA analysis shows that the first two components account for 52.18% of the total variation. The dendrogram tree is divided into four main parts, roughly representing the respective populations, which is constructed using 22 R. irroratum individuals. 3. Putative Development and the transferability test of SSR makers, Fifteen microsatellite loci were developed and characterized in R. delavayi. The average allele number of these microsatellites was 4 per locus, ranging from 3 to 6. The ranges of expected (HE) and observed (HO) heterozygosities were 0.0365-0.7091 and 0.0263-0.9512, respectively. Seven loci (R-111, R-112, R-147, R-299, R-320, R-335, and R-544) deviated significantly from the HWE (P﹤0.01). No significant linkage disequilibrium was detected between locus pairs except for three locus pairs: R-299 and R-544, R-166 and R-320, R-111 and R-320. Cross-species amplification in R. agastum, R. decorum, and R. irroratum showed that a subset of these markers holds promise for congeneric species study.4. ITS, matK, trnH-psbA and rbcL sequences. R. delavayi has six sites different from that of R. decorum in its ITS region, whereas R. agastum reveals double peaks at the corresponding sites and R. irroratum is identical to that of R. delavayi. The chloroplast segements show that some R. irroratum individuals share the same haplotype with R. delavayi and others share them with R. decorum.","jscount":"1","jsurl":"/simple-search?field1=all&rpp=10&accurate=false&advanced=false&sort_by=2&isNonaffiliated=false&search_type=-1&query1=Helianthus%2Bannuus%2BL&order=desc&&fq=dc.project.title_filter%3AThrough%5C+investigating%5C+sympatric%5C+distribution%5C+of%5C+Rhododendron%5C+irroratum%2C%5C+examing%5C+the%5C+variation%5C+of%5C+floral%5C+characters%5C+and%5C+sequencing%5C+the%5C+ITS%5C+and%5C+other%5C+chloroplast%5C+segements%2C%5C+we%5C+find%5C+that%5C+%5C%281%5C%29%5C+R.%5C+irroratum%5C+might%5C+be%5C+of%5C+hybrid%5C+origin%2C%5C+with%5C+its%5C+maternal%5C+parent%5C+R.%5C+delavayi%5C+or%5C+R.%5C+decorum.%5C+%5C%282%5C%29%5C+The%5C+ancestral%5C+haplotype%5C+of%5C+R.%5C+irroratum%5C+might%5C+be%5C+identical%5C+to%5C+that%5C+of%5C+R.%5C+decorum%2C%5C+and%5C+it%5C+is%5C+under%5C+ongoing%5C+introgression%5C+from%5C+R.%5C+delavayi.%5C+1.%5C+The%5C+natural%5C+distribution%2C%5C+Seven%5C+distribution%5C+sites%5C+of%5C+R.%5C+irroratum%5C+in%5C+Guizhou%5C+and%5C+Yunnan%5C+province%5C+were%5C+investigated.%5C+The%5C+result%5C+shows%5C+that%5C+R.%5C+irroratum%5C+is%5C+sympatric%5C+with%5C+R.%5C+delavayi%2C%5C+R.%5C+decorum%5C+and%5C+R.%5C+agastum.%5C+R.%5C+delavayi%5C+is%5C+widespread%5C+across%5C+the%5C+above%5C-mentioned%5C+seven%5C+sites%2C%5C+whereas%5C+R.%5C+agastum%5C+is%5C+scarce%5C+in%5C+DaPingDi%2C%5C+HuaDianBa%5C+and%5C+HeQing%5C+sites.%5C+R.%5C+irroratum%5C+and%5C+R.%5C+agastum%5C+distribute%5C+at%5C+the%5C+higher%5C+elevation%5C+compared%5C+to%5C+that%5C+of%5C+R.%5C+decorum%2C%5C+while%5C+R.%5C+delavayi%5C+is%5C+of%5C+widespread%5C+distribution%5C+across%5C+both%5C+regions%5C+of%5C+R.%5C+decorum%5C+and%5C+R.%5C+irroratum.2.%5C+Floral%5C+variation%5C+of%5C+R.%5C+irroratum%5C+among%5C+populations%2C%5C+The%5C+floral%5C+characters%5C+remain%5C+vary%5C+within%5C+and%5C+among%5C+populations%5C+except%5C+for%5C+the%5C+stamen%5C+number%5C+and%5C+the%5C+petal%5C+number.%5C+Seven%5C+floral%5C+characters%5C+correlates%5C+with%5C+each%5C+other%5C+among%5C+populations%2C%5C+of%5C+28%5C+different%5C+combinations%2C%5C+26%5C+reveal%5C+significant%5C+correlation%2C%5C+and%5C+23%5C+extremely%5C+significant%5C+correlation.%5C+The%5C+PCA%5C+analysis%5C+shows%5C+that%5C+the%5C+first%5C+two%5C+components%5C+account%5C+for%5C+52.18%25%5C+of%5C+the%5C+total%5C+variation.%5C+The%5C+dendrogram%5C+tree%5C+is%5C+divided%5C+into%5C+four%5C+main%5C+parts%2C%5C+roughly%5C+representing%5C+the%5C+respective%5C+populations%2C%5C+which%5C+is%5C+constructed%5C+using%5C+22%5C+R.%5C+irroratum%5C+individuals.%5C+3.%5C+Putative%5C+Development%5C+and%5C+the%5C+transferability%5C+test%5C+of%5C+SSR%5C+makers%2C%5C+Fifteen%5C+microsatellite%5C+loci%5C+were%5C+developed%5C+and%5C+characterized%5C+in%5C+R.%5C+delavayi.%5C+The%5C+average%5C+allele%5C+number%5C+of%5C+these%5C+microsatellites%5C+was%5C+4%5C+per%5C+locus%2C%5C+ranging%5C+from%5C+3%5C+to%5C+6.%5C+The%5C+ranges%5C+of%5C+expected%5C+%5C%28HE%5C%29%5C+and%5C+observed%5C+%5C%28HO%5C%29%5C+heterozygosities%5C+were%5C+0.0365%5C-0.7091%5C+and%5C+0.0263%5C-0.9512%2C%5C+respectively.%5C+Seven%5C+loci%5C+%5C%28R%5C-111%2C%5C+R%5C-112%2C%5C+R%5C-147%2C%5C+R%5C-299%2C%5C+R%5C-320%2C%5C+R%5C-335%2C%5C+and%5C+R%5C-544%5C%29%5C+deviated%5C+significantly%5C+from%5C+the%5C+HWE%5C+%5C%28P%EF%B9%A40.01%5C%29.%5C+No%5C+significant%5C+linkage%5C+disequilibrium%5C+was%5C+detected%5C+between%5C+locus%5C+pairs%5C+except%5C+for%5C+three%5C+locus%5C+pairs%5C%3A%5C+R%5C-299%5C+and%5C+R%5C-544%2C%5C+R%5C-166%5C+and%5C+R%5C-320%2C%5C+R%5C-111%5C+and%5C+R%5C-320.%5C+Cross%5C-species%5C+amplification%5C+in%5C+R.%5C+agastum%2C%5C+R.%5C+decorum%2C%5C+and%5C+R.%5C+irroratum%5C+showed%5C+that%5C+a%5C+subset%5C+of%5C+these%5C+markers%5C+holds%5C+promise%5C+for%5C+congeneric%5C+species%5C+study.4.%5C+ITS%2C%5C+matK%2C%5C+trnH%5C-psbA%5C+and%5C+rbcL%5C+sequences.%5C+R.%5C+delavayi%5C+has%5C+six%5C+sites%5C+different%5C+from%5C+that%5C+of%5C+R.%5C+decorum%5C+in%5C+its%5C+ITS%5C+region%2C%5C+whereas%5C+R.%5C+agastum%5C+reveals%5C+double%5C+peaks%5C+at%5C+the%5C+corresponding%5C+sites%5C+and%5C+R.%5C+irroratum%5C+is%5C+identical%5C+to%5C+that%5C+of%5C+R.%5C+delavayi.%5C+The%5C+chloroplast%5C+segements%5C+show%5C+that%5C+some%5C+R.%5C+irroratum%5C+individuals%5C+share%5C+the%5C+same%5C+haplotype%5C+with%5C+R.%5C+delavayi%5C+and%5C+others%5C+share%5C+them%5C+with%5C+R.%5C+decorum."},{"jsname":"Trigonobalanus doichangensis is an endangered plant. In this paper, the megasporogenesis and development of female gametophyte, seed morphological traits and seed germination, seed conservation, micropropagation and acclimatization of this species were studied. Combined with the published results of cytology, molecular genetics and other researches,the mechanisms of extinction, basic biology and technology of germplasm conservation and acclimatization of T. doichangensis were discussed. The main results are summarized as follows:1. Megasporogenesis and development of female gametophyte，Stamens exist under the stigma of T. doichangensis, and the pollen is aborted on the later development stage of pistil, therefore, the pistillate flower in function is hermaphrodite flower in morphology. The ovule is anatropous, bitegmic and crassinucellate. The primary archesporium is hypodermal and single-celled and the sporogenous cell of the nucellus functions directly as a megaspore mother cell which goes meiosis to form a linear tetrad. The chalazal megaspore of the tetrad is functional. The development of embryo sac conforms to the polygonum type. There are six ovules in the ovary of T. doichangensis, and only one develops into a seed in normal fruits. In the process of megasporogenesis and development of female gametophyte, there are several links of abortion, and 93.3% of mature embryo sacs is aborted.2. Morphological characters and germination of seeds，Most of the variation occurred among individual trees within populations in seed morphological traits (length, width and 1000-seed weight) and germination-related indices (germination percentage, germination index and vigor index). In addition, the variation in percentage of well-developed seeds among populations and among individual trees within populations is equal, each accounting for 48%. Each of seed morphological traits has significantly positive correlation with each other (p < 0.01), but they have no significant correlation with percentage of well-developed seeds and germination-related indices. In the same batch of seeds of T. doichangensis, there are light-colored and dark-colored seed coats, and development of light-colored seeds is significantly poorer than that of dark-colored seeds.The sensitivity of seeds to high temperature varys in different stages of seed imbibition. In each stage, heat acclimatization don’t increase germination percentage, germination index and fresh weight of seedlings. If the distilled water is substituted by solution of SA during seed imbibition, seed germination and germination index after heat shock are not significantly different from control, but they are significantly higher than that of other treatments. Moreover, when the seeds are treatmented with SA, the fresh weight of seedlings is significantly higher than that of control and other treatments.3. Seed conservation，Seeds of T. doichangensis belong to orthodox seeds which can tolerate certain level of dehydration. The condition of low temperature and low water content of seeds is conducive to seed conservation.Germination of fresh seeds shows significant variation among populations, howerer, germination of the seeds after storage for one year in room temperature shows no significant variation among populations.High temperature and high relative humidity damages the seeds more severely than high temperature does. In addition, low water content of seeds enable the seeds to be more tolerant to high temperature.The electrical conductivity, dehydrogenase activity and germination percentage have no significant correlation with each other.4. Micropropagation and in vitro conservation，Cotyledonary nodes are a kind of efficient explants. Low salt media are conducive to shoot propagation and root induction.The maximum multiplication rate (20-25 shoots/explant within 4 months) is achieved on quarter-strength Murashige and Skoog (1/4 MS) medium supplemented with 1 mg·L-1 6-benzyladenine (6-BA) and 0.05 mg·L-1 α-naphthaleneacetic acid (NAA).Rooting is promoted by auxins, however, IBA alone or low concentrations of NAA are preferable due to small amount of callus induced. The research has established an efficient protocol for micropropagation of T. doichangensis, and it provides technology support for in vitro conservation of special germplasm of the species.5. Acclimatization，Quercus variabilis, Cyclobalanopsis glaucoides and T. doichangensis belong to the family of Fagaceae, and the natural distribution ranges of the 3 species are decreasing in turn. The research suggests that the ranges of temperature tolerance of the 3 species are decreasing corresponding to their distribution ranges.The high and low semi-lethal temperature of one-year old T. doichangensis is 49.5℃ and -5℃ respectively. It suggests that T. doichangensis has a wide range of basic temperature tolerance. Short-term heat and cold acclimatization cannot expand the range of temperature tolerance. It can be inferred that T. doichangensis may lack induced tolerance to temperature. Under proper conditions, ABA can increase the cold tolerance, and SA can increase the heat tolerance of leaf discs of T. 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now, little data about the plant reproductive characters and ecological adaptation have been documented in the species-rich Sino-Himalaya region. Anemone rivularis (Ranunculaceae), mainly occurs in this area, and is of particular interest for its unique flower heliotropic movement and sex allocation strategy. In this study, we investigated the reproductive biology and adaptation mechanism of A. rivularis on the Yulong Snow Mountain Lijiang, northwestern Yunnan. The main results were summarized as follows: 1 Reproductive biology, The mating system, flowering phenology, floral morphology and pollination efficiency were examined in Anemone rivularis. This species is a perennial plant with hermaphroditic flowers, and its inflorescence is an acropetal cyme with protogynous flowers. In contrast to some self-incompatible species reported in Anemone, our results proved that A. rivularis was self-compatible. The seed set under natural pollination was more than 70%, indicating that there was no pollen limitation. Meanwhile, the seed set of artificial-cross-pollinated flowers was significantly higher than that of artificial-self-pollinated flowers, suggesting that the mixed mating system of A. rivularis was based on cross-pollination, and the results also supported a favor of outcrossing reproductive strategy for perennial herbs as some previous reports. Clearly, the reproductive strategy of A. rivularis prefer to cross-pollination in the alpine Sino-Himalayan region, in order to improve the reproductive fitness. 2 Flower heliotropism, The flower heliotropic movement mechanism, influences and adaptive significance were investigated in Anemone rivularis. The results indicated that under natural conditions, a treatment of pistils and stamens removal, flowers of A. rivularis retained accurately sun-tracking behavior through daytime, and the petals were found to close in the evening; but flowers would lose heliotropic movement if tepals were removed, with peduncles keeping a vertical orientation. This indicated that the tepals were crucial for heliotropic behavior. The flower heliotropism of A. rivularis was sensitive to blue light frequencies rather than red frequencies, suggesting that the light signal must be received by tepals, which driving the peduncles to bend due to differential cell elongation along the two sides of peduncle. Furthermore, there was a close relationship between diurnal heliotropic movements and temperature of flower interior in A. rivularis. Flowers with tepals could provide a relatively narrow range of temperatures, in comparison with flowers lacking tepals, in order to maintain reproductive organs in functional floral temperature range. Our study demonstrated that both the development of pistils and stamens and the visiting of insects could benefit from flower heliotropism in A. rivularis.3 Sex allocation, Floral traits, male and female functions, reproductive fitness, and sex allocation hypotheses were assessed in intra-inflorescence of Anemone rivularis. Though the inflorescence showed an acropetal flower-opening sequence as well as in many flowering species (early flowers are proximal and late flowers are distal), it engaged different sex allocation strategy. Our observations documented that the late-opening flowers of each inflorescence produce significantly more ovules and fewer pollen grains compared to early-opening flowers, and the pollen:ovule ratio (P:O) declined obviously from primary flower position to tertiary flower position, suggesting that later flowers would tend to favor female-bias investment. The nature-pollinating seed set among flower positions was constant, and there was no resource trade-off between flower size and sexual organs in this species, and the first-removal treatment did not lead to a significant increase in seed set of flowers in the later position. Thus, early-opening flower may not represent a significant competitor for resources with late-opening flowers on the same inflorescence, suggesting that the pattern of floral design and floral display may be determined prior to flowering and is inalterable by resources during flowering. So the female-biased allocation of distal flowers in A. rivularis may be resulted from the the selection by variation in the mating environment.","jscount":"1","jsurl":"/simple-search?field1=all&rpp=10&accurate=false&advanced=false&sort_by=2&isNonaffiliated=false&search_type=-1&query1=Helianthus%2Bannuus%2BL&order=desc&&fq=dc.project.title_filter%3AUntil%5C+now%2C%5C+little%5C+data%5C+about%5C+the%5C+plant%5C+reproductive%5C+characters%5C+and%5C+ecological%5C+adaptation%5C+have%5C+been%5C+documented%5C+in%5C+the%5C+species%5C-rich%5C+Sino%5C-Himalaya%5C+region.%5C+Anemone%5C+rivularis%5C+%5C%28Ranunculaceae%5C%29%2C%5C+mainly%5C+occurs%5C+in%5C+this%5C+area%2C%5C+and%5C+is%5C+of%5C+particular%5C+interest%5C+for%5C+its%5C+unique%5C+flower%5C+heliotropic%5C+movement%5C+and%5C+sex%5C+allocation%5C+strategy.%5C+In%5C+this%5C+study%2C%5C+we%5C+investigated%5C+the%5C+reproductive%5C+biology%5C+and%5C+adaptation%5C+mechanism%5C+of%5C+A.%5C+rivularis%5C+on%5C+the%5C+Yulong%5C+Snow%5C+Mountain%5C+Lijiang%2C%5C+northwestern%5C+Yunnan.%5C+The%5C+main%5C+results%5C+were%5C+summarized%5C+as%5C+follows%5C%3A%5C+1%5C+Reproductive%5C+biology%2C%5C+The%5C+mating%5C+system%2C%5C+flowering%5C+phenology%2C%5C+floral%5C+morphology%5C+and%5C+pollination%5C+efficiency%5C+were%5C+examined%5C+in%5C+Anemone%5C+rivularis.%5C+This%5C+species%5C+is%5C+a%5C+perennial%5C+plant%5C+with%5C+hermaphroditic%5C+flowers%2C%5C+and%5C+its%5C+inflorescence%5C+is%5C+an%5C+acropetal%5C+cyme%5C+with%5C+protogynous%5C+flowers.%5C+In%5C+contrast%5C+to%5C+some%5C+self%5C-incompatible%5C+species%5C+reported%5C+in%5C+Anemone%2C%5C+our%5C+results%5C+proved%5C+that%5C+A.%5C+rivularis%5C+was%5C+self%5C-compatible.%5C+The%5C+seed%5C+set%5C+under%5C+natural%5C+pollination%5C+was%5C+more%5C+than%5C+70%25%2C%5C+indicating%5C+that%5C+there%5C+was%5C+no%5C+pollen%5C+limitation.%5C+Meanwhile%2C%5C+the%5C+seed%5C+set%5C+of%5C+artificial%5C-cross%5C-pollinated%5C+flowers%5C+was%5C+significantly%5C+higher%5C+than%5C+that%5C+of%5C+artificial%5C-self%5C-pollinated%5C+flowers%2C%5C+suggesting%5C+that%5C+the%5C+mixed%5C+mating%5C+system%5C+of%5C+A.%5C+rivularis%5C+was%5C+based%5C+on%5C+cross%5C-pollination%2C%5C+and%5C+the%5C+results%5C+also%5C+supported%5C+a%5C+favor%5C+of%5C+outcrossing%5C+reproductive%5C+strategy%5C+for%5C+perennial%5C+herbs%5C+as%5C+some%5C+previous%5C+reports.%5C+Clearly%2C%5C+the%5C+reproductive%5C+strategy%5C+of%5C+A.%5C+rivularis%5C+prefer%5C+to%5C+cross%5C-pollination%5C+in%5C+the%5C+alpine%5C+Sino%5C-Himalayan%5C+region%2C%5C+in%5C+order%5C+to%5C+improve%5C+the%5C+reproductive%5C+fitness.%5C+2%5C+Flower%5C+heliotropism%2C%5C+The%5C+flower%5C+heliotropic%5C+movement%5C+mechanism%2C%5C+influences%5C+and%5C+adaptive%5C+significance%5C+were%5C+investigated%5C+in%5C+Anemone%5C+rivularis.%5C+The%5C+results%5C+indicated%5C+that%5C+under%5C+natural%5C+conditions%2C%5C+a%5C+treatment%5C+of%5C+pistils%5C+and%5C+stamens%5C+removal%2C%5C+flowers%5C+of%5C+A.%5C+rivularis%5C+retained%5C+accurately%5C+sun%5C-tracking%5C+behavior%5C+through%5C+daytime%2C%5C+and%5C+the%5C+petals%5C+were%5C+found%5C+to%5C+close%5C+in%5C+the%5C+evening%5C%3B%5C+but%5C+flowers%5C+would%5C+lose%5C+heliotropic%5C+movement%5C+if%5C+tepals%5C+were%5C+removed%2C%5C+with%5C+peduncles%5C+keeping%5C+a%5C+vertical%5C+orientation.%5C+This%5C+indicated%5C+that%5C+the%5C+tepals%5C+were%5C+crucial%5C+for%5C+heliotropic%5C+behavior.%5C+The%5C+flower%5C+heliotropism%5C+of%5C+A.%5C+rivularis%5C+was%5C+sensitive%5C+to%5C+blue%5C+light%5C+frequencies%5C+rather%5C+than%5C+red%5C+frequencies%2C%5C+suggesting%5C+that%5C+the%5C+light%5C+signal%5C+must%5C+be%5C+received%5C+by%5C+tepals%2C%5C+which%5C+driving%5C+the%5C+peduncles%5C+to%5C+bend%5C+due%5C+to%5C+differential%5C+cell%5C+elongation%5C+along%5C+the%5C+two%5C+sides%5C+of%5C+peduncle.%5C+Furthermore%2C%5C+there%5C+was%5C+a%5C+close%5C+relationship%5C+between%5C+diurnal%5C+heliotropic%5C+movements%5C+and%5C+temperature%5C+of%5C+flower%5C+interior%5C+in%5C+A.%5C+rivularis.%5C+Flowers%5C+with%5C+tepals%5C+could%5C+provide%5C+a%5C+relatively%5C+narrow%5C+range%5C+of%5C+temperatures%2C%5C+in%5C+comparison%5C+with%5C+flowers%5C+lacking%5C+tepals%2C%5C+in%5C+order%5C+to%5C+maintain%5C+reproductive%5C+organs%5C+in%5C+functional%5C+floral%5C+temperature%5C+range.%5C+Our%5C+study%5C+demonstrated%5C+that%5C+both%5C+the%5C+development%5C+of%5C+pistils%5C+and%5C+stamens%5C+and%5C+the%5C+visiting%5C+of%5C+insects%5C+could%5C+benefit%5C+from%5C+flower%5C+heliotropism%5C+in%5C+A.%5C+rivularis.3%5C+Sex%5C+allocation%2C%5C+Floral%5C+traits%2C%5C+male%5C+and%5C+female%5C+functions%2C%5C+reproductive%5C+fitness%2C%5C+and%5C+sex%5C+allocation%5C+hypotheses%5C+were%5C+assessed%5C+in%5C+intra%5C-inflorescence%5C+of%5C+Anemone%5C+rivularis.%5C+Though%5C+the%5C+inflorescence%5C+showed%5C+an%5C+acropetal%5C+flower%5C-opening%5C+sequence%5C+as%5C+well%5C+as%5C+in%5C+many%5C+flowering%5C+species%5C+%5C%28early%5C+flowers%5C+are%5C+proximal%5C+and%5C+late%5C+flowers%5C+are%5C+distal%5C%29%2C%5C+it%5C+engaged%5C+different%5C+sex%5C+allocation%5C+strategy.%5C+Our%5C+observations%5C+documented%5C+that%5C+the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Project","dc.project.title_filter")'>
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Boron in plants: deficiency and toxicity
期刊论文
出版物, 3111, 期号: 0, 页码: 1—24
Authors:
Juan J. Camacho-Cristóbal
;
Jesús Rexach
;
Agustín González-Fontes
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Submit date：2017/07/21
New and Antifungal Diterpenoids of Sunflower against Gray Mold
期刊论文
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 2023, 卷号: 71, 期号: 44, 页码: 16647-16656
Authors:
Zhao,Yun
;
Wang,Zi-Jiao
;
Wang,Chang-Bin
;
Tan,Bang-Yin
;
Luo,Xiao-Dong
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Submit date：2024/07/10
sunflower receptacle
Helianthus annuus L
diterpenoids
antifungal bioactivity
Botrytiscinerea
membrane integrity
OCCURRING TERPENE DERIVATIVES
KAURENE DERIVATIVES
ACID
TRACHYLOBANE
CONSTITUENTS
EXTRACT
KAURANE
Transcriptomes of Saussurea (Asteraceae) Provide Insights into High-Altitude Adaptation
期刊论文
PLANTS-BASEL, 2021, 卷号: 10, 期号: 8, 页码: 1715
Authors:
Zhang,Xu
;
Sun,Yanxia
;
Landis,Jacob B.
;
Shen,Jun
;
Zhang,Huajie
;
Kuang,Tianhui
;
Sun,Wenguang
;
Sun,Jiao
;
Tiamiyu,Bashir B.
;
Deng,Tao
;
Sun,Hang
;
Wang,Hengchang
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Submit date：2022/04/02
high-altitude adaptation
Saussurea
transcriptomes
positive selection
extreme environments
lineage-specific genes
RNA-SEQ
HENGDUAN MOUNTAINS
STRESS ACCLIMATION
SEQUENCE ALIGNMENT
HIGH ELEVATIONS
METABOLISM
GENOME
ANNOTATION
EVOLUTION
SELECTION
Demographic history and local adaptation of Myripnois dioica (Asteraceae) provide insight on plant evolution in northern China flora
期刊论文
ECOLOGY AND EVOLUTION, 2021, 卷号: 11, 期号: 12, 页码: 8000-8013
Authors:
Lin,Nan
;
Landis,Jacob B.
;
Sun,Yanxia
;
Huang,Xianhan
;
Zhang,Xu
;
Liu,Qun
;
Zhang,Huajie
;
Sun,Hang
;
Wang,Hengchang
;
Deng,Tao
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demographic history
effective population size
genomic variations
local adaption
Myripnois dioica
RAD‐
seq
LANDSCAPE GENOMICS
POPULATION-GENETICS
CLIMATE-CHANGE
PHYLOGEOGRAPHY
TREE
DIFFERENTIATION
DIVERGENCE
SOFTWARE
TOOL
PALAEOVEGETATION
Chemical constituents of Euphorbia peplus
期刊论文
BIOCHEMICAL SYSTEMATICS AND ECOLOGY, 2021, 卷号: 98, 页码: 104307
Authors:
Min,Xiao-Yan
;
Chen,Yan-Ni
;
Li,Dong-Mei
;
Mu,Qiu-Yan
;
Zhang,Zun-Ting
;
Fang,Xin
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Euphorbia peplus
Diterpene
Bisnorsesquiterpene
Monoterpene
Chemotaxonomy
JATROPHANE DITERPENOIDS
GUIDED ISOLATION
LOLIOLIDE
LATEX
多花蒿抗肝癌活性成分研究
学位论文
, 2020
Authors:
唐爽
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Submit date：2023/11/02
菟丝子与寄主间蛋白质大规模转运研究
学位论文
, 2020
Authors:
刘念
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Submit date：2023/11/02
Biofilm forming rhizobacteria enhance growth and salt tolerance in sunflower plants by stimulating antioxidant enzymes activity
期刊论文
PLANT PHYSIOLOGY AND BIOCHEMISTRY, 2020
Authors:
Yasmeen, Tahira
;
Ahmad, Aqeel
;
Arif, Muhammad Saleem
;
Mubin, Muhammad
;
Rehman, Khadija
;
Shahzad, Sher Muhammad
;
Iqbal, Shahid
;
Rizwan, Muhammad
;
Ali, Shafaqat
;
Alyemeni, Mohammed Nasser
;
Wijaya, Leonard
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Submit date：2021/01/05
Overexpression of SlMDHAR in transgenic tobacco increased salt stress tolerance involving S-nitrosylation regulation
期刊论文
PLANT SCIENCE, 2020
Authors:
Qi, Qi
;
Dong, Yanyan
;
Liang, Yuanlin
;
Li, Kunzhi
;
Xu, Huini
;
Sun, Xudong
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Submit date：2021/01/05
Refined families of Dothideomycetes: Dothideomycetidae and Pleosporomycetidae
期刊论文
MYCOSPHERE, 2020
Authors:
Hongsanan, S.
;
Hyde, K. D.
;
Phookamsak, R.
;
Wanasinghe, D. N.
;
McKenzie, E. H. C.
;
Sarma, V. V.
;
Boonmee, S.
;
Luecking, R.
;
Bhat, D. J.
;
Liu, N. G.
;
Tennakoon, D. S.
;
Pem, D. S.
;
Karunarathna, A.
;
Jiang, S. H.
;
Jones, E. B. G.
;
Phillips, A. J. L.
;
Manawasinghe, I. S.
;
Tibpromma, S.
;
Jayasiri, S. C.
;
Sandamali, D. S.
;
Jayawardena, R. S.
;
Wijayawardene, N. N.
;
Ekanayaka, A. H.
;
Jeewon, R.
;
Lu, Y. Z.
;
Dissanayake, A. J.
;
Zeng, X. Y.
;
Luo, Z. L.
;
Tian, Q.
;
Phukhamsakda, C.
;
Thambugala, K. M.
;
Dai, D. Q.
;
Chethana, K. W. T.
;
Samarakoon, M. C.
;
Ertz, D.
;
Bao, D. F.
;
Doilom, M.
;
Liu, J. K.
;
Perez-Ortega, S.
;
Suija, A.
;
Senwanna, C.
;
Wijesinghe, S. N.
;
Konta, S.
;
Niranjan, M.
;
Zhang, S. N.
;
Ariyawansa, H. A.
;
Jiang, H. B.
;
Zhang, J. F.
;
Norphanphoun, C.
;
de Silva, N., I
;
Thiyagaraja, V
;
Zhang, H.
;
Bezerra, J. D. P.
;
Miranda-Gonzalez, R.
;
Aptroot, A.
;
Kashiwadani, H.
;
Harishchandra, D.
;
Serusiaux, E.
;
Aluthmuhandiram, J. V. S.
;
Abeywickrama, P. D.
;
Devadatha, B.
;
Wu, H. X.
;
Moon, K. H.
;
Gueidan, C.
;
Schumm, F.
;
Bundhun, D.
;
Mapook, A.
;
Monkai, J.
;
Chomnunti, P.
;
Suetrong, S.
;
Chaiwan, N.
;
Dayarathne, M. C.
;
Yang, J.
;
Rathnayaka, A. R.
;
Bhunjun, C. S.
;
Xu, J. C.
;
Zheng, J. S.
;
Liu, G. L.
;
Feng, Y.
;
Xie, N.
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Submit date：2021/01/05
