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资助项目
0.05). For some populations, germination capacity in 12-h photoperiod was significantly higher than that in completed darkness（W-FD: P < 0.01, W-JD: P < 0.05）.Genetic variation within and among six populations was assessed using AFLP markers. Genetic diversity was higher at species level (PPL = 69.19%, HE = 0.221) than at population level (PPL = 26.22%, HE = 0.095, Is =0.140), and populations in southeast Yunnan were strongly differentiated from those in southwest Yunnan (Nei’s GST = 0.575; FST = 0.655). UPGMA analysis demonstrated a clear genetic division between the two populations from DeHong (SW Yunnan; D-JD and D-HG) and the four from WenShan (SE Yunnan; W-FD, W-LH, W-ML, and W-MG). Within-population genetic variation was significantly correlated with population isolation (r(PPL) = -0.94, P = 0.006; r(HE) = -0.85, P = 0.032; r(Is) = -0.87, P = 0.025), but not with population size (r(PPL) = 0.63, P = 0.178; r(HE) = 0.54, P = 0.268; r(Is) = 0.56, P = 0.249).","jscount":"1","jsurl":"/simple-search?field1=all&field=dc.project.fundingorganization_filter&advanced=false&query1=WOOD%2BANATOMY&&fq=dc.project.title_filter%3ACraigia%5C+yunnanensis%5C+W.%5C+W.%5C+Smith%5C+%5C%26%5C+W.%5C+E.%5C+Evans%5C+%5C%28Tiliaceae%5C%29%5C+is%5C+an%5C+endangered%5C+deciduous%5C+tree%5C+species%5C+which%5C+has%5C+high%5C+scientific%5C+and%5C+economic%5C+value.%5C+C.%5C+yunnanensis%5C+is%5C+seriously%5C+threatened%5C+and%5C+has%5C+been%5C+pushed%5C+to%5C+the%5C+verge%5C+of%5C+extinction%5C+due%5C+to%5C+vegetation%5C+destruction%5C+in%5C+China%5C+and%5C+consequent%5C+contraction%5C+of%5C+its%5C+distribution.%5C+Hence%2C%5C+it%5C+was%5C+listed%5C+as%5C+a%5C+nationally%5C+rare%5C+and%5C+endangered%5C+plant%5C+in%5C+1999%5C+and%5C+has%5C+also%5C+been%5C+proposed%5C+as%5C+a%5C+second%5C-ranked%5C+plant%5C+for%5C+national%5C+protection%5C+in%5C+China%5C+and%5C+included%5C+in%5C+IUCN%5C+red%5C+list.%5C+As%5C+a%5C+scientifically%5C+important%5C+and%5C+valued%5C+tree%5C+species%5C+with%5C+endangered%5C+status%2C%5C+the%5C+wild%5C+populations%5C+of%5C+C.%5C+yunnanensis%5C+therefore%5C+represent%5C+is%5C+a%5C+genetic%5C+resource%5C+that%5C+must%5C+be%5C+conserved.%5C+To%5C+provide%5C+basic%5C+information%5C+for%5C+its%5C+conservation%2C%5C+the%5C+population%5C+dynamics%5C+and%5C+population%5C+size%5C+structures%2C%5C+pollination%5C+biology%5C+and%5C+breeding%5C+system%2C%5C+eleven%5C+fitness%5C-related%5C+characters%5C+and%5C+the%5C+genetic%5C+variability%5C+based%5C+on%5C+AFLP%5C+were%5C+comprehensively%5C+studied.%5C+The%5C+main%5C+results%5C+are%5C+summarized%5C+as%5C+follows%5C%3A%5C+A%5C+total%5C+of%5C+six%5C+wild%5C+populations%5C+of%5C+C.%5C+yunnanensis%5C+were%5C+found%5C+in%5C+two%5C+disjunct%5C+regions%5C+of%5C+Yunnan%2C%5C+i.e.%5C+WenShan%5C+%5C%28SE%5C+Yunnan%5C%29%5C+and%5C+DeHong%5C+%5C%28SW%5C+Yunnan%5C%29%2C%5C+from%5C+2005%5C+to%5C+2007.%5C+Additionally%2C%5C+in%5C+all%5C+but%5C+one%5C+of%5C+the%5C+populations%5C+we%5C+detected%2C%5C+mature%5C+trees%5C+were%5C+felled%5C+between%5C+2005%5C+and%5C+2007%2C%5C+so%5C+destruction%5C+of%5C+most%5C+of%5C+these%5C+populations%5C+is%5C+ongoing.%5C+Across%5C+the%5C+six%5C+populations%5C+of%5C+extant%5C+C.%5C+yunnanensis%5C+found%5C+during%5C+our%5C+study%2C%5C+the%5C+total%5C+number%5C+of%5C+mature%5C+%5C%28reproductive%5C%29%5C+individuals%5C+detected%5C+was%5C+584%5C+in%5C+2007%EF%BC%8Cplus%5C+larger%5C+numbers%5C+of%5C+seedling%5C+and%5C+resprouts%5C+from%5C+cut%5C+trunks.%5C+The%5C+result%5C+of%5C+surveying%5C+Population%5C+structure%5C+showed%5C+that%5C+there%5C+are%5C+two%5C+regeneration%5C+types%5C+which%5C+are%5C+seedlings%5C+and%5C+sprouts.%5C+Seedlings%5C+occurred%5C+abundantly%5C+in%5C+gaps%5C+or%5C+open%5C+areas%5C+and%5C+the%5C+size%5C+class%5C+frequency%5C+distributions%5C+were%5C+often%5C+discontinuous%2C%5C+and%5C+the%5C+same%5C+general%5C+pattern%5C+occurred%5C+in%5C+all%5C+the%5C+investigated%5C+populations%5C+for%5C+juveniles%5C+and%5C+adults.%5C+The%5C+numbers%5C+of%5C+seed%5C-origin%5C+individuals%5C+did%5C+however%5C+decline%5C+sharply%5C+with%5C+increasing%5C+size%2C%5C+indicating%5C+a%5C+high%5C+mortality%5C+rate%5C+going%5C+from%5C+seedling%5C+to%5C+sapling%5C+stage%5C+may%5C+be%5C+a%5C+problem%5C+for%5C+this%5C+species.%5C+Additionally%2C%5C+the%5C+cash%5C+crop%5C+cultivation%5C+and%5C+logging%5C+seriously%5C+threaten%5C+the%5C+survival%5C+of%5C+the%5C+species.%5C+We%5C+conducted%5C+field%5C+observations%5C+and%5C+artificial%5C+pollination%5C+experiments%5C+on%5C+the%5C+floral%5C+biology%2C%5C+pollination%5C+process%5C+and%5C+breeding%5C+system%5C+of%5C+Craigia%5C+yunnanensis%5C+in%5C+Fadou%2C%5C+Xichou%5C+county%5C+of%5C+Yunnan%5C+province.%5C+The%5C+lifespan%5C+of%5C+a%5C+single%5C+hermaphrodite%5C+flower%5C+is%5C+approximately%5C+3%5C-4%5C+days.%5C+A%5C+cyme%5C+has%5C+2%5C-9%5C+flowered.%5C+The%5C+flowering%5C+period%5C+of%5C+an%5C+inflorescence%5C+is%5C+usually%5C+5%5C-14%5C+days.%5C+The%5C+flowers%5C+of%5C+C.%5C+yunnanensis%5C+were%5C+protandrous.%5C+The%5C+stamens%5C+were%5C+within%5C+petal%5C-like%5C+staminodes%5C+in%5C+the%5C+opening%5C+flowers%5C+until%5C+the%5C+flower%5C+withered.%5C+Without%5C+touchment%2C%5C+the%5C+bractlike%5C+staminodes%5C+can%E2%80%99t%5C+open.%5C+Self%5C-pollination%5C+was%5C+partially%5C+avoided%5C+by%5C+temporal%5C+and%5C+spatial%5C+isolation%5C+of%5C+male%5C+and%5C+female%5C+organs%5C+within%5C+the%5C+same%5C+flower.%5C+However%2C%5C+autogamous%5C+and%5C+geitonogamous%5C+pollination%5C+is%5C+unavoidable%5C+because%5C+of%5C+the%5C+large%5C+number%5C+of%5C+flowers%5C+on%5C+a%5C+single%5C+tree%5C+and%5C+the%5C+action%5C+of%5C+pollinators.%5C+The%5C+values%5C+of%5C+both%5C+OCI%5C+%5C%28%E2%89%A54%5C%29%5C+and%5C+P%5C%2FO%5C+%5C%281381%5C%29%5C+and%5C+the%5C+results%5C+of%5C+bagging%5C+tests%5C+indicated%5C+there%5C+was%5C+no%5C+apomixes%5C+in%5C+C.%5C+yunnanensis%5C+and%5C+the%5C+breeding%5C+system%5C+of%5C+the%5C+species%5C+was%5C+outcrossing%5C+with%5C+partial%5C+self%5C-co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study can reveal important biological features of plants and answers to a certain degree in phylogeny and distribution of genetic materials and so forth. By hard working of cytologists, chromosome data of plants have been increased to a great abundance, but yet disorderly distributed in different magazines, which made researches based on the whole chromosome data of one taxon rarely launched. Scientific databases have become increasingly indispensable as researching data growing daily. As Cytological studies are booming in China, in order to fill the absence of digital and statistical data of plant chromosome researches and chromosome atlas, we started to develop a Chinese Seed Plants Chromosome Database, aiming to construct a database and start to record published chromosome data of Chinese seed plants. Based on this database, we chose the part of gymnosperms and gave a discussion to the features of its chromosomes’ evolution and variation. Cytological experiments have been applied to some important phyto-groups for phylogeny research and germplasm identification.Part I: The Chinese Seed Plants Chromosome Database and Discussion on the features of Gymnosperms chromosomes，1 The Chinese Seed Plants Chromosome Database，The frame of database was constructed by Microsoft Access 2003. 19 items of data were included in, they are: Chinese and Latin names of family, genus and species; plant pictures, mitosis metaphase and karyotype figures; morphological characteristics and distributions of the plant; chromosome numbers and basic numbers; karyotype formula; karyotype description; origin of the plant material; literature and the source of photos. In this database, data can be checked and shared easily by extracted out in species sorted interface or family sorted interface. 120 species in 29 genera and 10 families of Gymnospers have been collected and input to the database. In Angiosperms, 61 species in 10 genera of family Magnoliaceae and 80 species in 3 genera of family Theaceae have been collected and input to the database.2 Discussion on the features of evolution and variation of Gymnosperms chromosomes，By data collection of the database, we analyzed chromosome features of the group Gymnosperm. Plants of Gymnosperm had been through a long historical evolution on earth, fossil records of which originated from the late Devonian period. Once an authoritative and major classification level in the plant kingdom, most Gymnosperms have been extinct unless conifers, cycads, Ginkgo and Getales. Three main features of Gymnosperm chromosomes are: relatively large chromosome, which can be recognized from figures in the database; constant chromosome numbers, in most families of Gymnosperm the basic chromosome number keeps a certain value; comparatively low variation, karyotype under family level differs a little. The variation of chromosomes in Gymnosperm is dominated by Robertsonian changes. Contrary to common variation type in Angiosperms, the variation from high unsymmetric karyotype to low unsymmetric karyotype was found in existence in Gymnosperm.Part II: cytology research on some important phyto-groups，3 Karyomorphology of three species in the order Huerteales and their phylogenetic implications，The karyomorphology of three species in Dipentodon (Dipentodontaceae), Perrottetia (Celastraceae), and Tapiscia (Tapisciaceae), namely Dipentodon sinicus, Perrottetia racemosa, and Tapiscia sinensis, was investigated in the study. Recent molecular research has discovered close relationships among these three genera, which has led to the establishment of the order Huerteales with Perrottetia being placed in Dipentodontaceae. Herein we report the chromosome numbers of D. sinicus and P. racemosa for the first time, and present their karyotype formulas as 2n = 34 = 22sm + 12st (D. sinicus), 2n = 20 = 11m + 9sm (P. racemosa), and 2n = 30 = 22m(2SAT) + 8sm (T. sinensis). Asymmetry of their karyotypes is categorized to be Type 3B in D. sinicus, Type 2A in P. racemosa, and Type 2A in T. sinensis. Each of the species shows special cytological features. Compared with Perrottetia, Dipentodon has a different basic chromosome number, a higher karyotype asymmetry, and different karyomorphology of its interphase nuclei, mitotic prophase, and metaphase. Thus, on the basis of these results, we have reservations regarding the suggestion of placing Dipentodon and Perrottetia together in the family Dipentodontaceae.4 Genomic analyses of intergeneric hybrids between Michelia crassipes and M. calcicola by GISH，Genomic in situ hybridization (GISH) is becoming the method of choice for identifying parental chromosomes in interspecific hybrids. Interspecific F1 hybrid between Michelia crassipes and M. calcicola, tow highly ornamental species in Michelia of Magnolicaceae, has been analized by double-colored GISH with its parents’ genome as the probe. Research gave the results that the chromosome number of the F1 hybrid is 2n=38 as the same of species in Michelia and other genera in Magnoliaceae, the basic chromosome is x=19, the karyotype formula is 2n=38=32m+6sm, and the asymmetry of karyotype is 1B type. Based on chromosome data of Michelia in our database, the karyotype of this genus is featured mostly by metacentric chromosomes and submetacentric chromosomes. In Mechelia, the variation range of submetacentric chromosomes is 4 to 18 and of the karyotype asymmetry is 1A to 2B type. Both the karyotype and karyotype asymmetry type of F1 hybrid is among the variation range of Michelia. The figure of GISH showed that all the 38 chromosomes of F1 hybrid have crossing parental signals, and signal on the no.1 and no.7 chromosome showed differences, which proved that both the parental genome have been transmitted to and recombinated in F1 hybrid.","jscount":"1","jsurl":"/simple-search?field1=all&field=dc.project.fundingorganization_filter&advanced=false&query1=WOOD%2BANATOMY&&fq=dc.project.title_filter%3ACytology%5C+study%5C+can%5C+reveal%5C+important%5C+biological%5C+features%5C+of%5C+plants%5C+and%5C+answers%5C+to%5C+a%5C+certain%5C+degree%5C+in%5C+phylogeny%5C+and%5C+distribution%5C+of%5C+genetic%5C+materials%5C+and%5C+so%5C+forth.%5C+By%5C+hard%5C+working%5C+of%5C+cytologists%2C%5C+chromosome%5C+data%5C+of%5C+plants%5C+have%5C+been%5C+increased%5C+to%5C+a%5C+great%5C+abundance%2C%5C+but%5C+yet%5C+disorderly%5C+distributed%5C+in%5C+different%5C+magazines%2C%5C+which%5C+made%5C+researches%5C+based%5C+on%5C+the%5C+whole%5C+chromosome%5C+data%5C+of%5C+one%5C+taxon%5C+rarely%5C+launched.%5C+Scientific%5C+databases%5C+have%5C+become%5C+increasingly%5C+indispensable%5C+as%5C+researching%5C+data%5C+growing%5C+daily.%5C+As%5C+Cytological%5C+studies%5C+are%5C+booming%5C+in%5C+China%2C%5C+in%5C+order%5C+to%5C+fill%5C+the%5C+absence%5C+of%5C+digital%5C+and%5C+statistical%5C+data%5C+of%5C+plant%5C+chromosome%5C+researches%5C+and%5C+chromosome%5C+atlas%2C%5C+we%5C+started%5C+to%5C+develop%5C+a%5C+Chinese%5C+Seed%5C+Plants%5C+Chromosome%5C+Database%2C%5C+aiming%5C+to%5C+construct%5C+a%5C+database%5C+and%5C+start%5C+to%5C+record%5C+published%5C+chromosome%5C+data%5C+of%5C+Chinese%5C+seed%5C+plants.%5C+Based%5C+on%5C+this%5C+database%2C%5C+we%5C+chose%5C+the%5C+part%5C+of%5C+gymnosperms%5C+and%5C+gave%5C+a%5C+discussion%5C+to%5C+the%5C+features%5C+of%5C+its%5C+chromosomes%E2%80%99%5C+evolution%5C+and%5C+variation.%5C+Cytological%5C+experiments%5C+have%5C+been%5C+applied%5C+to%5C+some%5C+important%5C+phyto%5C-groups%5C+for%5C+phylogeny%5C+research%5C+and%5C+germplasm%5C+identification.Part%5C+I%5C%3A%5C+The%5C+Chinese%5C+Seed%5C+Plants%5C+Chromosome%5C+Database%5C+and%5C+Discussion%5C+on%5C+the%5C+features%5C+of%5C+Gymnosperms%5C+chromosomes%EF%BC%8C1%5C+%C2%A0The%5C+Chinese%5C+Seed%5C+Plants%5C+Chromosome%5C+Database%EF%BC%8CThe%5C+frame%5C+of%5C+database%5C+was%5C+constructed%5C+by%5C+Microsoft%5C+Access%5C+2003.%5C+19%5C+items%5C+of%5C+data%5C+were%5C+included%5C+in%2C%5C+they%5C+are%5C%3A%5C+Chinese%5C+and%5C+Latin%5C+names%5C+of%5C+family%2C%5C+genus%5C+and%5C+species%5C%3B%5C+plant%5C+pictures%2C%5C+mitosis%5C+metaphase%5C+and%5C+karyotype%5C+figures%5C%3B%5C+morphological%5C+characteristics%5C+and%5C+distributions%5C+of%5C+the%5C+plant%5C%3B%5C+chromosome%5C+numbers%5C+and%5C+basic%5C+numbers%5C%3B%5C+karyotype%5C+formula%5C%3B%5C+karyotype%5C+description%5C%3B%5C+origin%5C+of%5C+the%5C+plant%5C+material%5C%3B%5C+literature%5C+and%5C+the%5C+source%5C+of%5C+photos.%5C+In%5C+this%5C+database%2C%5C+data%5C+can%5C+be%5C+checked%5C+and%5C+shared%5C+easily%5C+by%5C+extracted%5C+out%5C+in%5C+species%5C+sorted%5C+interface%5C+or%5C+family%5C+sorted%5C+interface.%5C+120%5C+species%5C+in%5C+29%5C+genera%5C+and%5C+10%5C+families%5C+of%5C+Gymnospers%5C+have%5C+been%5C+collected%5C+and%5C+input%5C+to%5C+the%5C+database.%5C+In%5C+Angiosperms%2C%5C+61%5C+species%5C+in%5C+10%5C+genera%5C+of%5C+family%5C+Magnoliaceae%5C+and%5C+80%5C+species%5C+in%5C+3%5C+genera%5C+of%5C+family%5C+Theaceae%5C+have%5C+been%5C+collected%5C+and%5C+input%5C+to%5C+the%5C+database.2%5C+Discussion%5C+on%5C+the%5C+features%5C+of%5C+evolution%5C+and%5C+variation%5C+of%5C+Gymnosperms%5C+chromosomes%EF%BC%8CBy%5C+data%5C+collection%5C+of%5C+the%5C+database%2C%5C+we%5C+analyzed%5C+chromosome%5C+features%5C+of%5C+the%5C+group%5C+Gymnosperm.%5C+Plants%5C+of%5C+Gymnosperm%5C+had%5C+been%5C+through%5C+a%5C+long%5C+historical%5C+evolution%5C+on%5C+earth%2C%5C+fossil%5C+records%5C+of%5C+which%5C+originated%5C+from%5C+the%5C+late%5C+Devonian%5C+period.%5C+Once%5C+an%5C+authoritative%5C+and%5C+major%5C+classification%5C+level%5C+in%5C+the%5C+plant%5C+kingdom%2C%5C+most%5C+Gymnosperms%5C+have%5C+been%5C+extinct%5C+unless%5C+conifers%2C%5C+cycads%2C%5C+Ginkgo%5C+and%5C+Getales.%5C+Three%5C+main%5C+features%5C+of%5C+Gymnosperm%5C+chromosomes%5C+are%5C%3A%5C+relatively%5C+large%5C+chromosome%2C%5C+which%5C+can%5C+be%5C+recognized%5C+from%5C+figures%5C+in%5C+the%5C+database%5C%3B%5C+constant%5C+chromosome%5C+numbers%2C%5C+in%5C+most%5C+families%5C+of%5C+Gymnosperm%5C+the%5C+basic%5C+chromosome%5C+number%5C+keeps%5C+a%5C+certain%5C+value%5C%3B%5C+comparatively%5C+low%5C+variation%2C%5C+karyotype%5C+under%5C+family%5C+level%5C+differs%5C+a%5C+little.%5C+The%5C+variation%5C+of%5C+chromosomes%5C+in%5C+Gymnosperm%5C+is%5C+dominated%5C+by%5C+Robertsonian%5C+changes.%5C+Contrary%5C+to%5C+common%5C+variation%5C+type%5C+in%5C+Angiosperms%2C%5C+the%5C+variation%5C+from%5C+high%5C+unsymmetric%5C+karyotype%5C+to%5C+low%5C+unsymmetric%5C+karyotype%5C+was%5C+found%5C+in%5C+existence%5C+in%5C+Gymnosperm.Part%5C+II%5C%3A%5C+cytology%5C+research%5C+on%5C+some%5C+important%5C+phyto%5C-groups%EF%BC%8C3%5C+Karyomorphology%5C+of%5C+three%5C+species%5C+in%5C+the%5C+order%5C+Huerteales%5C+and%5C+their%5C+phylogenetic%5C+implicati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Academic Exchange Service (DAAD)","jscount":"1","jsurl":"/simple-search?field1=all&field=dc.project.fundingorganization_filter&advanced=false&query1=WOOD%2BANATOMY&&fq=dc.project.title_filter%3AGerman%5C+Academic%5C+Exchange%5C+Service%5C+%5C%28DAAD%5C%29"},{"jsname":"Glory Light International Fellowship for Chinese Botanists at Missouri Botanical Garden","jscount":"1","jsurl":"/simple-search?field1=all&field=dc.project.fundingorganization_filter&advanced=false&query1=WOOD%2BANATOMY&&fq=dc.project.title_filter%3AGlory%5C+Light%5C+International%5C+Fellowship%5C+for%5C+Chinese%5C+Botanists%5C+at%5C+Missouri%5C+Botanical%5C+Garden"},{"jsname":"Kunming Institute of Botany, Chinese Academy of Sciences","jscount":"1","jsurl":"/simple-search?field1=all&field=dc.project.fundingorganization_filter&advanced=false&query1=WOOD%2BANATOMY&&fq=dc.project.title_filter%3AKunming%5C+Institute%5C+of%5C+Botany%2C%5C+Chinese%5C+Academy%5C+of%5C+Sciences"},{"jsname":"Mae Fah Luang University[666713]","jscount":"1","jsurl":"/simple-search?field1=all&field=dc.project.fundingorganization_filter&advanced=false&query1=WOOD%2BANATOMY&&fq=dc.project.title_filter%3AMae%5C+Fah%5C+Luang%5C+University%5C%5B666713%5C%5D"},{"jsname":"National Natural Science Foundation of China (NSFC)[41030212]","jscount":"1","jsurl":"/simple-search?field1=all&field=dc.project.fundingorganization_filter&advanced=false&query1=WOOD%2BANATOMY&&fq=dc.project.title_filter%3ANational%5C+Natural%5C+Science%5C+Foundation%5C+of%5C+China%5C+%5C%28NSFC%5C%29%5C%5B41030212%5C%5D"},{"jsname":"National Natural Science Foundation of China (NSFC)[41272007]","jscount":"1","jsurl":"/simple-search?field1=all&field=dc.project.fundingorganization_filter&advanced=false&query1=WOOD%2BANATOMY&&fq=dc.project.title_filter%3ANational%5C+Natural%5C+Science%5C+Foundation%5C+of%5C+China%5C+%5C%28NSFC%5C%29%5C%5B41272007%5C%5D"},{"jsname":"National Natural Science Foundation of China[31400196]","jscount":"1","jsurl":"/simple-search?field1=all&field=dc.project.fundingorganization_filter&advanced=false&query1=WOOD%2BANATOMY&&fq=dc.project.title_filter%3ANational%5C+Natural%5C+Science%5C+Foundation%5C+of%5C+China%5C%5B31400196%5C%5D"},{"jsname":"National Natural Science Foundation of China[31628002]","jscount":"1","jsurl":"/simple-search?field1=all&field=dc.project.fundingorganization_filter&advanced=false&query1=WOOD%2BANATOMY&&fq=dc.project.title_filter%3ANational%5C+Natural%5C+Science%5C+Foundation%5C+of%5C+China%5C%5B31628002%5C%5D"},{"jsname":"National Natural Science Foundation of China[31670342]","jscount":"1","jsurl":"/simple-search?field1=all&field=dc.project.fundingorganization_filter&advanced=false&query1=WOOD%2BANATOMY&&fq=dc.project.title_filter%3ANational%5C+Natural%5C+Science%5C+Foundation%5C+of%5C+China%5C%5B31670342%5C%5D"},{"jsname":"Pilot Work of the fourth National survey on Chinese Materia Medica Resources[2017-2019]","jscount":"1","jsurl":"/simple-search?field1=all&field=dc.project.fundingorganization_filter&advanced=false&query1=WOOD%2BANATOMY&&fq=dc.project.title_filter%3APilot%5C+Work%5C+of%5C+the%5C+fourth%5C+National%5C+survey%5C+on%5C+Chinese%5C+Materia%5C+Medica%5C+Resources%5C%5B2017%5C-2019%5C%5D"},{"jsname":"Plants in the genus of Pedicularis L. have high values in horticulture and some species are medicinal plants. However, they have a reputation for being uncultivable, which is an obstacle for their exploitation and utilization. In this dissertation, the hemiparasitism of Pedicularis species was studied systematically for the first time, and the successful cultivation of three Pedicularis species (Pedicularis densispica，Pedicularis cephalantha and Pedicularis rex) throughout all life stages was achieved for the first time. With several representative species as main study materials, a series of experiments on seed germination, vegetation survey in Pedicularis communities, and pot cultivation were carried out. We studied Pedicularis parasitic habit systematically and aimed to achieve progress in the cultivation of Pedicularis species based on the understanding of their parasitic habit. The main results are as follows:1. The dormancy showed by the tested Pedicularis species was non-deep physiological dormancy. Seed dormancy could be overcome by moist-chilling and GA3. Cold stratification for 30-60 days, or 500-1000 mg/L GA3 were the optimal treatments for germination percentage, and stratification for 15 days, or 1000 mg/L GA3 were the optimal treatments for mean germination time. The combination of cold stratification and GA3 were more effective measures to promote seed germination. The optimal germination conditions varied with species, while as a whole, the highest germination percentages were obtained from treatments of 500-1000 mg/L GA3 followed by 15-30 days stratification, and the lowest values of mean germination time were obtained from treatments of 100-1000mg/L GA3 followed by 15-day stratification. Seed germination was not the obstacle for the cultivation of Pedicularis species.2. Host range and host selectivity of a Pedicularis species were studied systematically for the first time. Examinations of haustorial connections revealed that P. densispica had a wide host range, and it can form haustorial connections on the roots of 33 species belonging to 14 families. Compositae (8 species), Gramineae (5 species) and Leguminosae (5 species) species comprised major hosts. In addition, self-parasitism was observed. Haustoria were non-randomly distributed among host species, suggesting that there was some host selectivity. P. densispica generally preferred species in the families of Gramineae and Cyperaceae. The results of association analysis and correlation analysis based on vegetation survey supported the result of examinations of haustorial connections. And correlation analysis was a better way to test host selectivity.3. This is the first report for the performance of Pedicularis species in cultivation throughout all life stages (from seeds to seeds). The high dependence of Pedicularis specieson host plants and their host preference were demonstrated in this study. Pedicularis speciesstrongly depended on host presence, while host plants were essential to Pedicularis speciesnot for survival but for proper development. Different Pedicularis species preferred to different hosts. Host defoliation was a useful promoting measure for the cultivation of Pedicularis species. Pedicularis species reduced the performance of host plants. With the assistance of hosts, three Pedicularis species were cultivated successfully and they retained high horticulture 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