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中国科学院昆明植物研究所知识管理系统
Knowledge Management System of Kunming Institute of Botany,CAS
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0.05). For some populations, germination capacity in 12-h photoperiod was significantly higher than that in completed darkness（W-FD: P < 0.01, W-JD: P < 0.05）.Genetic variation within and among six populations was assessed using AFLP markers. Genetic diversity was higher at species level (PPL = 69.19%, HE = 0.221) than at population level (PPL = 26.22%, HE = 0.095, Is =0.140), and populations in southeast Yunnan were strongly differentiated from those in southwest Yunnan (Nei’s GST = 0.575; FST = 0.655). UPGMA analysis demonstrated a clear genetic division between the two populations from DeHong (SW Yunnan; D-JD and D-HG) and the four from WenShan (SE Yunnan; W-FD, W-LH, W-ML, and W-MG). Within-population genetic variation was significantly correlated with population isolation (r(PPL) = -0.94, P = 0.006; r(HE) = -0.85, P = 0.032; r(Is) = -0.87, P = 0.025), but not with population size (r(PPL) = 0.63, P = 0.178; r(HE) = 0.54, P = 0.268; r(Is) = 0.56, P = 0.249).","jscount":"1","jsurl":"/simple-search?field1=all&field=dc.project.fundingorganization_filter&advanced=false&query1=General-principles&&fq=dc.project.title_filter%3ACraigia%5C+yunnanensis%5C+W.%5C+W.%5C+Smith%5C+%5C%26%5C+W.%5C+E.%5C+Evans%5C+%5C%28Tiliaceae%5C%29%5C+is%5C+an%5C+endangered%5C+deciduous%5C+tree%5C+species%5C+which%5C+has%5C+high%5C+scientific%5C+and%5C+economic%5C+value.%5C+C.%5C+yunnanensis%5C+is%5C+seriously%5C+threatened%5C+and%5C+has%5C+been%5C+pushed%5C+to%5C+the%5C+verge%5C+of%5C+extinction%5C+due%5C+to%5C+vegetation%5C+destruction%5C+in%5C+China%5C+and%5C+consequent%5C+contraction%5C+of%5C+its%5C+distribution.%5C+Hence%2C%5C+it%5C+was%5C+listed%5C+as%5C+a%5C+nationally%5C+rare%5C+and%5C+endangered%5C+plant%5C+in%5C+1999%5C+and%5C+has%5C+also%5C+been%5C+proposed%5C+as%5C+a%5C+second%5C-ranked%5C+plant%5C+for%5C+national%5C+protection%5C+in%5C+China%5C+and%5C+included%5C+in%5C+IUCN%5C+red%5C+list.%5C+As%5C+a%5C+scientifically%5C+important%5C+and%5C+valued%5C+tree%5C+species%5C+with%5C+endangered%5C+status%2C%5C+the%5C+wild%5C+populations%5C+of%5C+C.%5C+yunnanensis%5C+therefore%5C+represent%5C+is%5C+a%5C+genetic%5C+resource%5C+that%5C+must%5C+be%5C+conserved.%5C+To%5C+provide%5C+basic%5C+information%5C+for%5C+its%5C+conservation%2C%5C+the%5C+population%5C+dynamics%5C+and%5C+population%5C+size%5C+structures%2C%5C+pollination%5C+biology%5C+and%5C+breeding%5C+system%2C%5C+eleven%5C+fitness%5C-related%5C+characters%5C+and%5C+the%5C+genetic%5C+variability%5C+based%5C+on%5C+AFLP%5C+were%5C+comprehensively%5C+studied.%5C+The%5C+main%5C+results%5C+are%5C+summarized%5C+as%5C+follows%5C%3A%5C+A%5C+total%5C+of%5C+six%5C+wild%5C+populations%5C+of%5C+C.%5C+yunnanensis%5C+were%5C+found%5C+in%5C+two%5C+disjunct%5C+regions%5C+of%5C+Yunnan%2C%5C+i.e.%5C+WenShan%5C+%5C%28SE%5C+Yunnan%5C%29%5C+and%5C+DeHong%5C+%5C%28SW%5C+Yunnan%5C%29%2C%5C+from%5C+2005%5C+to%5C+2007.%5C+Additionally%2C%5C+in%5C+all%5C+but%5C+one%5C+of%5C+the%5C+populations%5C+we%5C+detected%2C%5C+mature%5C+trees%5C+were%5C+felled%5C+between%5C+2005%5C+and%5C+2007%2C%5C+so%5C+destruction%5C+of%5C+most%5C+of%5C+these%5C+populations%5C+is%5C+ongoing.%5C+Across%5C+the%5C+six%5C+populations%5C+of%5C+extant%5C+C.%5C+yunnanensis%5C+found%5C+during%5C+our%5C+study%2C%5C+the%5C+total%5C+number%5C+of%5C+mature%5C+%5C%28reproductive%5C%29%5C+individuals%5C+detected%5C+was%5C+584%5C+in%5C+2007%EF%BC%8Cplus%5C+larger%5C+numbers%5C+of%5C+seedling%5C+and%5C+resprouts%5C+from%5C+cut%5C+trunks.%5C+The%5C+result%5C+of%5C+surveying%5C+Population%5C+structure%5C+showed%5C+that%5C+there%5C+are%5C+two%5C+regeneration%5C+types%5C+which%5C+are%5C+seedlings%5C+and%5C+sprouts.%5C+Seedlings%5C+occurred%5C+abundantly%5C+in%5C+gaps%5C+or%5C+open%5C+areas%5C+and%5C+the%5C+size%5C+class%5C+frequency%5C+distributions%5C+were%5C+often%5C+discontinuous%2C%5C+and%5C+the%5C+same%5C+general%5C+pattern%5C+occurred%5C+in%5C+all%5C+the%5C+investigated%5C+populations%5C+for%5C+juveniles%5C+and%5C+adults.%5C+The%5C+numbers%5C+of%5C+seed%5C-origin%5C+individuals%5C+did%5C+however%5C+decline%5C+sharply%5C+with%5C+increasing%5C+size%2C%5C+indicating%5C+a%5C+high%5C+mortality%5C+rate%5C+going%5C+from%5C+seedling%5C+to%5C+sapling%5C+stage%5C+may%5C+be%5C+a%5C+problem%5C+for%5C+this%5C+species.%5C+Additionally%2C%5C+the%5C+cash%5C+crop%5C+cultivation%5C+and%5C+logging%5C+seriously%5C+threaten%5C+the%5C+survival%5C+of%5C+the%5C+species.%5C+We%5C+conducted%5C+field%5C+observations%5C+and%5C+artificial%5C+pollination%5C+experiments%5C+on%5C+the%5C+floral%5C+biology%2C%5C+pollination%5C+process%5C+and%5C+breeding%5C+system%5C+of%5C+Craigia%5C+yunnanensis%5C+in%5C+Fadou%2C%5C+Xichou%5C+county%5C+of%5C+Yunnan%5C+province.%5C+The%5C+lifespan%5C+of%5C+a%5C+single%5C+hermaphrodite%5C+flower%5C+is%5C+approximately%5C+3%5C-4%5C+days.%5C+A%5C+cyme%5C+has%5C+2%5C-9%5C+flowered.%5C+The%5C+flowering%5C+period%5C+of%5C+an%5C+inflorescence%5C+is%5C+usually%5C+5%5C-14%5C+days.%5C+The%5C+flowers%5C+of%5C+C.%5C+yunnanensis%5C+were%5C+protandrous.%5C+The%5C+stamens%5C+were%5C+within%5C+petal%5C-like%5C+staminodes%5C+in%5C+the%5C+opening%5C+flowers%5C+until%5C+the%5C+flower%5C+withered.%5C+Without%5C+touchment%2C%5C+the%5C+bractlike%5C+staminodes%5C+can%E2%80%99t%5C+open.%5C+Self%5C-pollination%5C+was%5C+partially%5C+avoided%5C+by%5C+temporal%5C+and%5C+spatial%5C+isolation%5C+of%5C+male%5C+and%5C+female%5C+organs%5C+within%5C+the%5C+same%5C+flower.%5C+However%2C%5C+autogamous%5C+and%5C+geitonogamous%5C+pollination%5C+is%5C+unavoidable%5C+because%5C+of%5C+the%5C+large%5C+number%5C+of%5C+flowers%5C+on%5C+a%5C+single%5C+tree%5C+and%5C+the%5C+action%5C+of%5C+pollinators.%5C+The%5C+values%5C+of%5C+both%5C+OCI%5C+%5C%28%E2%89%A54%5C%29%5C+and%5C+P%5C%2FO%5C+%5C%281381%5C%29%5C+and%5C+the%5C+results%5C+of%5C+bagging%5C+tests%5C+indicated%5C+there%5C+was%5C+no%5C+apomixes%5C+in%5C+C.%5C+yunnanensis%5C+and%5C+the%5C+breeding%5C+system%5C+of%5C+the%5C+species%5C+was%5C+outcrossing%5C+with%5C+partial%5C+self%5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order to investigate the indole alkaloids, this thesis studied on the chemical constituents of two plants, Neolamarckia cadamba and Plumeria rubra. Forty–one compounds, including ten new compounds were isolated, and the biogenetic pathways of them were proposed.Chapter 1: Study on Chemical Constituents of Neolamarckia cadamba, Thirty–seven compounds were isolated from the leaves of the Neolamarckia cadamba, and thirty–three of them were identified, including ten new compounds. The structural types of these compounds are mainly monoterpenoid indole alkaloids. Among them, compounds 1, 2, 3, 4 and 5 are considered to be derived biogenetically from the corresponding glycosidic indole alkaloids by introducing the third nitrogen atom into their E ring respectively by the double Mannich–like condensation. It is the first sample that monoterpenoid indole alkaloids could be further modified by amino acids. There is also a novel rearrangement indole alkaloid (compound 6) isolated from this genus.Chapter 2: Study on Chemical Constituents of Plumeria rubra, Four compounds were isolated from the leaves of the Plumeria rubra, and all of them were identified as monoterpenoid indole alkaloids.Chapter 3: Review,Review on the plumeria type compounds, including the chemical structural types, the bioactivity and the plant sources","jscount":"1","jsurl":"/simple-search?field1=all&field=dc.project.fundingorganization_filter&advanced=false&query1=General-principles&&fq=dc.project.title_filter%3AIn%5C+order%5C+to%5C+investigate%5C+the%5C+indole%5C+alkaloids%2C%5C+this%5C+thesis%5C+studied%5C+on%5C+the%5C+chemical%5C+constituents%5C+of%5C+two%5C+plants%2C%5C+Neolamarckia%5C+cadamba%5C+and%5C+Plumeria%5C+rubra.%5C+Forty%E2%80%93one%5C+compounds%2C%5C+including%5C+ten%5C+new%5C+compounds%5C+were%5C+isolated%2C%5C+and%5C+the%5C+biogenetic%5C+pathways%5C+of%5C+them%5C+were%5C+proposed.Chapter%5C+1%5C%3A%5C+Study%5C+on%5C+Chemical%5C+Constituents%5C+of%5C+Neolamarckia%5C+cadamba%2C%5C+Thirty%E2%80%93seven%5C+compounds%5C+were%5C+isolated%5C+from%5C+the%5C+leaves%5C+of%5C+the%5C+Neolamarckia%5C+cadamba%2C%5C+and%5C+thirty%E2%80%93three%5C+of%5C+them%5C+were%5C+identified%2C%5C+including%5C+ten%5C+new%5C+compounds.%5C+The%5C+structural%5C+types%5C+of%5C+these%5C+compounds%5C+are%5C+mainly%5C+monoterpenoid%5C+indole%5C+alkaloids.%5C+Among%5C+them%2C%5C+compounds%5C+1%2C%5C+2%2C%5C+3%2C%5C+4%5C+and%5C+5%5C+are%5C+considered%5C+to%5C+be%5C+derived%5C+biogenetically%5C+from%5C+the%5C+corresponding%5C+glycosidic%5C+indole%5C+alkaloids%5C+by%5C+introducing%5C+the%5C+third%5C+nitrogen%5C+atom%5C+into%5C+their%5C+E%5C+ring%5C+respectively%5C+by%5C+the%5C+double%5C+Mannich%E2%80%93like%5C+condensation.%5C+It%5C+is%5C+the%5C+first%5C+sample%5C+that%5C+monoterpenoid%5C+indole%5C+alkaloids%5C+could%5C+be%5C+further%5C+modified%5C+by%5C+amino%5C+acids.%5C+There%5C+is%5C+also%5C+a%5C+novel%5C+rearrangement%5C+indole%5C+alkaloid%5C+%5C%28compound%5C+6%5C%29%5C+isolated%5C+from%5C+this%5C+genus.Chapter%5C+2%5C%3A%5C+Study%5C+on%5C+Chemical%5C+Constituents%5C+of%5C+Plumeria%5C+rubra%2C%5C+Four%5C+compounds%5C+were%5C+isolated%5C+from%5C+the%5C+leaves%5C+of%5C+the%5C+Plumeria%5C+rubra%2C%5C+and%5C+all%5C+of%5C+them%5C+were%5C+identified%5C+as%5C+monoterpenoid%5C+indole%5C+alkaloids.Chapter%5C+3%5C%3A%5C+Review%2CReview%5C+on%5C+the%5C+plumeria%5C+type%5C+compounds%2C%5C+including%5C+the%5C+chemical%5C+structural%5C+types%2C%5C+the%5C+bioactivity%5C+and%5C+the%5C+plant%5C+sources"},{"jsname":"In the present study, we focused on “Pterygiella complex”, included Pterygiella Oliver, Xizangia D.Y. Hong, Phtheirospermum Bunge ex Fischer & C.A. Meyer, and Pseudobartsia D.Y. Hong, which is endemic to Eastern Asia. Based on chloroplast and nuclear sequences, we explored their phylogeny relationships within Orobanchaceae, the species relations within Pterygiella, and fruit and seed morphology of traditional tribe Rhinantheae. The phylogeny of “Pterygiella complex” was reconstructed based on nuclear and chloroplast sequences within the family Orobanchaceae. The genera relationship within the complex was reconstructed based on chloroplast sequences of atpB-rbcL, atpH-I, psbA-trnH, rpl16, trnL-F and trnS-G. The results showed that “Pterygiella complex” was not a natural group and could be divided into two different clades. Clade I included most taxa, e.g. Pterygiella, Xizangia, Pseudobartsia, Phtheirospermum (exclude P. japonicum). The species of this clade were endemic to East-Himalaya and Hengduan Mountains region. Clade II included Phtheirospermum japonicum (Thunberg) Kanitz, which was a heterogeneous member in genus Phtheirospermum and should be treated as a new monotypic genus. The results supported that Pterygiella bartschioides Hand.-Mazz. and Phtheirospermum glandulosum Benth. should be elevated to genus level as Xizangia and Pseudobartsia, respectively.Furthermore, we focused on the genus Pterygiella to explore the species’ circumscription by molecular phylogeny, DNA barcodes and morphological studies. The results suggested that Pterygiella should divide into three clades. P. duclouxii was divided into clade I and clade II, and P. nigrescens was included the clade I of these P. duclouxii taxa, with which it shares eglandular hairs on the stem. Clade III included P. suffruticosa and P. cylindrica, while the level of inter- and intra-species variation in two species did not support their distinction. Therefore, P. suffruticosa should move into or considered as a variety of P. cylindrica. The form of stem, leaf veins and the indumentum of stems are key traits for circumscribing the species within the genus. By comparing the effectiveness with core DNA barcodes, ITS-2 can be used as suitable DNA barcode in the genus Pterygiella.Fruit and seed characteristics of 49 species in 21 genera of the tribe Rhinantheae and 9 species in 9 genera of Orobachaceae were examined. 25 characters were selected and analyzed by principal component analysis for discovering the systematic significances. The results suggested four main types and six subtypes were distinguished based on gross seed coat appearance, inner tangential wall and thickenings of radial wall. Fruit and seed data reflect the close relationships within “Pterygiella complex”. While, Xizangia was distinctly different from Pterygiella. Phtheirospermum tenuisectum was more similar to the member of section minutisepala within the genus Phtheiroseprmum. Phtheirospermum japonicum was heterogeneous within the genus Phtheirospermum. On the whole, fruit and seed data supported Xizangia and Pseudobartsia as a genus rank and Phtheirospermum japonicum was a heterogeneous member in 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