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中国科学院昆明植物研究所知识管理系统
Knowledge Management System of Kunming Institute of Botany,CAS
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0.05). For some populations, germination capacity in 12-h photoperiod was significantly higher than that in completed darkness（W-FD: P < 0.01, W-JD: P < 0.05）.Genetic variation within and among six populations was assessed using AFLP markers. Genetic diversity was higher at species level (PPL = 69.19%, HE = 0.221) than at population level (PPL = 26.22%, HE = 0.095, Is =0.140), and populations in southeast Yunnan were strongly differentiated from those in southwest Yunnan (Nei’s GST = 0.575; FST = 0.655). UPGMA analysis demonstrated a clear genetic division between the two populations from DeHong (SW Yunnan; D-JD and D-HG) and the four from WenShan (SE Yunnan; W-FD, W-LH, W-ML, and W-MG). Within-population genetic variation was significantly correlated with population isolation (r(PPL) = -0.94, P = 0.006; r(HE) = -0.85, P = 0.032; r(Is) = -0.87, P = 0.025), but not with population size (r(PPL) = 0.63, P = 0.178; r(HE) = 0.54, P = 0.268; r(Is) = 0.56, P = 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micholitzii complex is composed of 5 species: C. micholitzii Dyer, C. bifida (Dyer) K. D. Hill,C. longipetiolula D. Y. Wang, C. debaoensis Y. C. Zhong et C J. Chen, C. multipinnata C J. Chen et S. Y. Yang，and distributed from southwest China to central Vietnam and eastern Laos. Based on sequence data from two maternally inherited cpDNA and one biparentally nuclear DNA fragments, our study revealed the population genetic structure of C. micholitzii complex and explored the potential causes. The evolutionary and demographic histories were investigated. The genetic relationship among species in the complex was also clarified.The results were summarized as follows: 1. Phylogeographic analysis based on chloroplast sequences，We examined chloroplast sequence variation of the atpB-rbcLand psbA-trnHintergenic spacers in 27 populations of C. micholitzii complex, recovering 26 haplotypes. The average within-population diversity (HS = 0.140) was low while total diversity (HT = 0.911) was high. Population differentiation was also high(GST = 0.846, NST = 0.919), indicating significant phylogeographical structure (NST > GST,p < 0.001) and low levels of seed-based gene flow. C. debaoensis (Cycadaceae) is an endangered species restricted to the border of Guangxi and Yunnan province in southwest China. This species has been classified into two types: sand and karst, according to the soil matrix they grow on. We examined chloroplast sequence variation of the cpDNA sequences from 11 populations of this species. Significant population genetic differentiation was detected (GST= 0.684 and FST = 0.74160). There was marked genetic differentiation between populations in the sand and karst regions and no expansion was detected. Climate changes during glacial periods have had significant effects on the current distribution of cycads. The molecular phylogenetic data, together with the geographic distribution of the haplotypes, suggest that C. debaoensis experienced range contraction during glacial periods, and that the current populations are still confined to the original refugia in southwest China which have favorable habitats in glacial period. These results imply that small refugia were maintained in both sand and karst regions during the LGM (last glacial maximum). This species had no postglacial recolonization and only stayed in these refugia up to now. The low within-population diversity of C. debaoensis suggests that there were strong bottleneck events or founder effects within each separate region during the Quaternary climatic oscillations. Relatively high genetic and haplotype diversities were detected in the newly discovered populations, which located at intermediate locality of sand regions and had morphological variation; this is probably the consequence of the admixture of different haplotypes colonizing the area from separate sources. C. micholitzii occurs in the Annan Highlands in central Vietnam near the Laos border. C. bifida occurs in North Vietnam; its distribution extends across the border into adjacent localities in Guangxi and Yunnan in China. For the comparability between them,theywere considered as the same species C. micholitzii by many academicians. The cpDNA sequences from 11 populations showed that these very controversial species, C. micholitzii and C. bifida, is paraphyletic and should belong to the same species C. micholitzii. AMOVA analysis showed that the component of among-population within region/species (76.46%) was unexpectedly larger than the among-species/region component (14.97%), which also indicates that there is no justification for recognizing two species as C. micholitzii and C. bifida. This hypothesis was also supported by the geological data, especially the neotectonic history of the indo-china block, which started to move south since Oligocene and cause the geographic isolation of these two groups. Therefore, the most likely explanation to the phenotypic similarities between these two groups may be the retention of ancestral polymorphisms in the paraphyletic group due to incomplete lineage sorting. Furthermore, the similarities may also be ascribed to pollen-mediated gene flow among geographically proximate populations and/or phenotypic convergence under similar selection schemes in the same region. C.micholitzi had the higest genetic diversity (HT = 0.980,) and genetic differentiation (GST = 0.830, NST = 0.915) among the C. micholitzii complex. The high genetic diversity might be attributed to its long evolutionary history, highly diverse habitats. The ineffective mode of seed dispersal and dramatic neotectonic movement in the distribution range of this species could result in the high genetic differentiation. 2. Phylogeographic analysis based on nuclear ribosomal sequences, We sequenced the nrDNA ITS in all 27 populations sampled, 7 haplotypes were identified, among which C. micholitzii had 6, while C. multipinnata, C. longipetiolula and C. debaoensis shared the remaining one. Compared to chloroplast genes, nuclear genes had higher correlation between genetic and geographical distance, but lower interspecies differentiation (54.42% vs 25.24%). Phylogeographical structure of C. micholitzii and C.bifida based on ITS Variation was consistent with the morphology differentiation. This similar in nuclear gene should be ascribed to pollen-mediated gene flow among geographically proximate populations.Long-distance gene flow over the two groups was clearly interrupted, which brought on the nrDNA genetic differenciation between the geographically isolated groups, to a certain extent affected the morphological variation. 3. Interspecies relationships among Cycas micholitzii complex, We analysed chloroplast sequence variation of the atpB-rbcL and psbA-trnH intergenic spacers in 27 populations sampled of C. micholitzii complex, AMOVA analysis showed that the component of among-species/region component (59.21%). However, phylogenic analysis showed that the haplotypes of C. micholitzii complex couldn`t grouped into four clusters closely corresponding to the narrowly defined C. micholitzi, C. multipinnata, C. debaoensis and C. longipetiolula. We concluded that the conflict may result from several factors: firstly incomplete lineage sorting of C. micholitzii; secondly hybridization/introgression of sympatrically cycads, which would be supported by evidence base on nrDNA ITS sequences; thirdly intramolecular recombination in cpDNA of cycads; eventually the neotectonic movement in the distribution range of this species.","jscount":"1","jsurl":"/simple-search?field1=all&field=dc.citation.source_filter&advanced=false&query1=GENE%2BORGANIZATION&&fq=dc.project.title_filter%3ACycas%5C+micholitzii%5C+complex%5C+is%5C+composed%5C+of%5C+5%5C+species%5C%3A%5C+C.%5C+micholitzii%5C+Dyer%2C%5C+C.%5C+bifida%5C+%5C%28Dyer%5C%29%5C+K.%5C+D.%5C+Hill%2CC.%5C+longipetiolula%5C+D.%5C+Y.%5C+Wang%2C%5C+C.%5C+debaoensis%5C+Y.%5C+C.%5C+Zhong%5C+et%5C+C%5C+J.%5C+Chen%2C%5C+C.%5C+multipinnata%5C+C%5C+J.%5C+Chen%5C+et%5C+S.%5C+Y.%5C+Yang%EF%BC%8Cand%5C+distributed%5C+from%5C+southwest%5C+China%5C+to%5C+central%5C+Vietnam%5C+and%5C+eastern%5C+Laos.%5C+Based%5C+on%5C+sequence%5C+data%5C+from%5C+two%5C+maternally%5C+inherited%5C+cpDNA%5C+and%5C+one%5C+biparentally%5C+nuclear%5C+DNA%5C+fragments%2C%5C+our%5C+study%5C+revealed%5C+the%5C+population%5C+genetic%5C+structure%5C+of%5C+C.%5C+micholitzii%5C+complex%5C+and%5C+explored%5C+the%5C+potential%5C+causes.%5C+The%5C+evolutionary%5C+and%5C+demographic%5C+histories%5C+were%5C+investigated.%5C+The%5C+genetic%5C+relationship%5C+among%5C+species%5C+in%5C+the%5C+complex%5C+was%5C+also%5C+clarified.The%5C+results%5C+were%5C+summarized%5C+as%5C+follows%5C%3A%5C+1.%5C+Phylogeographic%5C+analysis%5C+based%5C+on%5C+chloroplast%5C+sequences%EF%BC%8CWe%5C+examined%5C+chloroplast%5C+sequence%5C+variation%5C+of%5C+the%5C+atpB%5C-rbcLand%5C+psbA%5C-trnHintergenic%5C+spacers%5C+in%5C+27%5C+populations%5C+of%5C+C.%5C+micholitzii%5C+complex%2C%5C+recovering%5C+26%5C+haplotypes.%5C+The%5C+average%5C+within%5C-population%5C+diversity%5C+%5C%28HS%5C+%3D%5C+0.140%5C%29%5C+was%5C+low%5C+while%5C+total%5C+diversity%5C+%5C%28HT%5C+%3D%5C+0.911%5C%29%5C+was%5C+high.%5C+Population%5C+differentiation%5C+was%5C+also%5C+high%5C%28GST%5C+%3D%5C+0.846%2C%5C+NST%5C+%3D%5C+0.919%5C%29%2C%5C+indicating%5C+significant%5C+phylogeographical%5C+structure%5C+%5C%28NST%5C+%3E%5C+GST%2Cp%5C+%3C%5C+0.001%5C%29%5C+and%5C+low%5C+levels%5C+of%5C+seed%5C-based%5C+gene%5C+flow.%5C+C.%5C+debaoensis%5C+%5C%28Cycadaceae%5C%29%5C+is%5C+an%5C+endangered%5C+species%5C+restricted%5C+to%5C+the%5C+border%5C+of%5C+Guangxi%5C+and%5C+Yunnan%5C+province%5C+in%5C+southwest%5C+China.%5C+This%5C+species%5C+has%5C+been%5C+classified%5C+into%5C+two%5C+types%5C%3A%5C+sand%5C+and%5C+karst%2C%5C+according%5C+to%5C+the%5C+soil%5C+matrix%5C+they%5C+grow%5C+on.%5C+We%5C+examined%5C+chloroplast%5C+sequence%5C+variation%5C+of%5C+the%5C+cpDNA%5C+sequences%5C+from%5C+11%5C+populations%5C+of%5C+this%5C+species.%5C+Significant%5C+population%5C+genetic%5C+differentiation%5C+was%5C+detected%5C+%5C%28GST%3D%5C+0.684%5C+and%5C+FST%5C+%3D%5C+0.74160%5C%29.%5C+There%5C+was%5C+marked%5C+genetic%5C+differentiation%5C+between%5C+populations%5C+in%5C+the%5C+sand%5C+and%5C+karst%5C+regions%5C+and%5C+no%5C+expansion%5C+was%5C+detected.%5C+Climate%5C+changes%5C+during%5C+glacial%5C+periods%5C+have%5C+had%5C+significant%5C+effects%5C+on%5C+the%5C+current%5C+distribution%5C+of%5C+cycads.%5C+The%5C+molecular%5C+phylogenetic%5C+data%2C%5C+together%5C+with%5C+the%5C+geographic%5C+distribution%5C+of%5C+the%5C+haplotypes%2C%5C+suggest%5C+that%5C+C.%5C+debaoensis%5C+experienced%5C+range%5C+contraction%5C+during%5C+glacial%5C+periods%2C%5C+and%5C+that%5C+the%5C+current%5C+populations%5C+are%5C+still%5C+confined%5C+to%5C+the%5C+original%5C+refugia%5C+in%5C+southwest%5C+China%5C+which%5C+have%5C+favorable%5C+habitats%5C+in%5C+glacial%5C+period.%5C+These%5C+results%5C+imply%5C+that%5C+small%5C+refugia%5C+were%5C+maintained%5C+in%5C+both%5C+sand%5C+and%5C+karst%5C+regions%5C+during%5C+the%5C+LGM%5C+%5C%28last%5C+glacial%5C+maximum%5C%29.%5C+This%5C+species%5C+had%5C+no%5C+postglacial%5C+recolonization%5C+and%5C+only%5C+stayed%5C+in%5C+these%5C+refugia%5C+up%5C+to%5C+now.%5C+The%5C+low%5C+within%5C-population%5C+diversity%5C+of%5C+C.%5C+debaoensis%5C+suggests%5C+that%5C+there%5C+were%5C+strong%5C+bottleneck%5C+events%5C+or%5C+founder%5C+effects%5C+within%5C+each%5C+separate%5C+region%5C+during%5C+the%5C+Quaternary%5C+climatic%5C+oscillations.%5C+Relatively%5C+high%5C+genetic%5C+and%5C+haplotype%5C+diversities%5C+were%5C+detected%5C+in%5C+the%5C+newly%5C+discovered%5C+populations%2C%5C+which%5C+located%5C+at%5C+intermediate%5C+locality%5C+of%5C+sand%5C+regions%5C+and%5C+had%5C+morphological%5C+variation%5C%3B%5C+this%5C+is%5C+probably%5C+the%5C+consequence%5C+of%5C+the%5C+admixture%5C+of%5C+different%5C+haplotypes%5C+colonizing%5C+the%5C+area%5C+from%5C+separate%5C+sources.%5C+%5C+C.%5C+micholitzii%5C+occurs%5C+in%5C+the%5C+Annan%5C+Highlands%5C+in%5C+central%5C+Vietnam%5C+near%5C+the%5C+Laos%5C+border.%5C+C.%5C+bifida%5C+occurs%5C+in%5C+North%5C+Vietnam%5C%3B%5C+its%5C+distribution%5C+extends%5C+across%5C+the%5C+border%5C+into%5C+adjacent%5C+localities%5C+in%5C+Guangxi%5C+and%5C+Yunnan%5C+in%5C+China.%5C+For%5C+the%5C+comparability%5C+between%5C+them%2Ctheywere%5C+considered%5C+as%5C+the%5C+same%5C+species%5C+C.%5C+micholitzii%5C+by%5C+many%5C+academicians.%5C+The%5C+cpDNA%5C+sequences%5C+from%5C+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study can reveal important biological features of plants and answers to a certain degree in phylogeny and distribution of genetic materials and so forth. By hard working of cytologists, chromosome data of plants have been increased to a great abundance, but yet disorderly distributed in different magazines, which made researches based on the whole chromosome data of one taxon rarely launched. Scientific databases have become increasingly indispensable as researching data growing daily. As Cytological studies are booming in China, in order to fill the absence of digital and statistical data of plant chromosome researches and chromosome atlas, we started to develop a Chinese Seed Plants Chromosome Database, aiming to construct a database and start to record published chromosome data of Chinese seed plants. Based on this database, we chose the part of gymnosperms and gave a discussion to the features of its chromosomes’ evolution and variation. Cytological experiments have been applied to some important phyto-groups for phylogeny research and germplasm identification.Part I: The Chinese Seed Plants Chromosome Database and Discussion on the features of Gymnosperms chromosomes，1 The Chinese Seed Plants Chromosome Database，The frame of database was constructed by Microsoft Access 2003. 19 items of data were included in, they are: Chinese and Latin names of family, genus and species; plant pictures, mitosis metaphase and karyotype figures; morphological characteristics and distributions of the plant; chromosome numbers and basic numbers; karyotype formula; karyotype description; origin of the plant material; literature and the source of photos. In this database, data can be checked and shared easily by extracted out in species sorted interface or family sorted interface. 120 species in 29 genera and 10 families of Gymnospers have been collected and input to the database. In Angiosperms, 61 species in 10 genera of family Magnoliaceae and 80 species in 3 genera of family Theaceae have been collected and input to the database.2 Discussion on the features of evolution and variation of Gymnosperms chromosomes，By data collection of the database, we analyzed chromosome features of the group Gymnosperm. Plants of Gymnosperm had been through a long historical evolution on earth, fossil records of which originated from the late Devonian period. Once an authoritative and major classification level in the plant kingdom, most Gymnosperms have been extinct unless conifers, cycads, Ginkgo and Getales. Three main features of Gymnosperm chromosomes are: relatively large chromosome, which can be recognized from figures in the database; constant chromosome numbers, in most families of Gymnosperm the basic chromosome number keeps a certain value; comparatively low variation, karyotype under family level differs a little. The variation of chromosomes in Gymnosperm is dominated by Robertsonian changes. Contrary to common variation type in Angiosperms, the variation from high unsymmetric karyotype to low unsymmetric karyotype was found in existence in Gymnosperm.Part II: cytology research on some important phyto-groups，3 Karyomorphology of three species in the order Huerteales and their phylogenetic implications，The karyomorphology of three species in Dipentodon (Dipentodontaceae), Perrottetia (Celastraceae), and Tapiscia (Tapisciaceae), namely Dipentodon sinicus, Perrottetia racemosa, and Tapiscia sinensis, was investigated in the study. Recent molecular research has discovered close relationships among these three genera, which has led to the establishment of the order Huerteales with Perrottetia being placed in Dipentodontaceae. Herein we report the chromosome numbers of D. sinicus and P. racemosa for the first time, and present their karyotype formulas as 2n = 34 = 22sm + 12st (D. sinicus), 2n = 20 = 11m + 9sm (P. racemosa), and 2n = 30 = 22m(2SAT) + 8sm (T. sinensis). Asymmetry of their karyotypes is categorized to be Type 3B in D. sinicus, Type 2A in P. racemosa, and Type 2A in T. sinensis. Each of the species shows special cytological features. Compared with Perrottetia, Dipentodon has a different basic chromosome number, a higher karyotype asymmetry, and different karyomorphology of its interphase nuclei, mitotic prophase, and metaphase. Thus, on the basis of these results, we have reservations regarding the suggestion of placing Dipentodon and Perrottetia together in the family Dipentodontaceae.4 Genomic analyses of intergeneric hybrids between Michelia crassipes and M. calcicola by GISH，Genomic in situ hybridization (GISH) is becoming the method of choice for identifying parental chromosomes in interspecific hybrids. Interspecific F1 hybrid between Michelia crassipes and M. calcicola, tow highly ornamental species in Michelia of Magnolicaceae, has been analized by double-colored GISH with its parents’ genome as the probe. Research gave the results that the chromosome number of the F1 hybrid is 2n=38 as the same of species in Michelia and other genera in Magnoliaceae, the basic chromosome is x=19, the karyotype formula is 2n=38=32m+6sm, and the asymmetry of karyotype is 1B type. Based on chromosome data of Michelia in our database, the karyotype of this genus is featured mostly by metacentric chromosomes and submetacentric chromosomes. In Mechelia, the variation range of submetacentric chromosomes is 4 to 18 and of the karyotype asymmetry is 1A to 2B type. Both the karyotype and karyotype asymmetry type of F1 hybrid is among the variation range of Michelia. The figure of GISH showed that all the 38 chromosomes of F1 hybrid have crossing parental signals, and signal on the no.1 and no.7 chromosome showed differences, which proved that both the parental genome have been transmitted to and recombinated in F1 hybrid.","jscount":"1","jsurl":"/simple-search?field1=all&field=dc.citation.source_filter&advanced=false&query1=GENE%2BORGANIZATION&&fq=dc.project.title_filter%3ACytology%5C+study%5C+can%5C+reveal%5C+important%5C+biological%5C+features%5C+of%5C+plants%5C+and%5C+answers%5C+to%5C+a%5C+certain%5C+degree%5C+in%5C+phylogeny%5C+and%5C+distribution%5C+of%5C+genetic%5C+materials%5C+and%5C+so%5C+forth.%5C+By%5C+hard%5C+working%5C+of%5C+cytologists%2C%5C+chromosome%5C+data%5C+of%5C+plants%5C+have%5C+been%5C+increased%5C+to%5C+a%5C+great%5C+abundance%2C%5C+but%5C+yet%5C+disorderly%5C+distributed%5C+in%5C+different%5C+magazines%2C%5C+which%5C+made%5C+researches%5C+based%5C+on%5C+the%5C+whole%5C+chromosome%5C+data%5C+of%5C+one%5C+taxon%5C+rarely%5C+launched.%5C+Scientific%5C+databases%5C+have%5C+become%5C+increasingly%5C+indispensable%5C+as%5C+researching%5C+data%5C+growing%5C+daily.%5C+As%5C+Cytological%5C+studies%5C+are%5C+booming%5C+in%5C+China%2C%5C+in%5C+order%5C+to%5C+fill%5C+the%5C+absence%5C+of%5C+digital%5C+and%5C+statistical%5C+data%5C+of%5C+plant%5C+chromosome%5C+researches%5C+and%5C+chromosome%5C+atlas%2C%5C+we%5C+started%5C+to%5C+develop%5C+a%5C+Chinese%5C+Seed%5C+Plants%5C+Chromosome%5C+Database%2C%5C+aiming%5C+to%5C+construct%5C+a%5C+database%5C+and%5C+start%5C+to%5C+record%5C+published%5C+chromosome%5C+data%5C+of%5C+Chinese%5C+seed%5C+plants.%5C+Based%5C+on%5C+this%5C+database%2C%5C+we%5C+chose%5C+the%5C+part%5C+of%5C+gymnosperms%5C+and%5C+gave%5C+a%5C+discussion%5C+to%5C+the%5C+features%5C+of%5C+its%5C+chromosomes%E2%80%99%5C+evolution%5C+and%5C+variation.%5C+Cytological%5C+experiments%5C+have%5C+been%5C+applied%5C+to%5C+some%5C+important%5C+phyto%5C-groups%5C+for%5C+phylogeny%5C+research%5C+and%5C+germplasm%5C+identification.Part%5C+I%5C%3A%5C+The%5C+Chinese%5C+Seed%5C+Plants%5C+Chromosome%5C+Database%5C+and%5C+Discussion%5C+on%5C+the%5C+features%5C+of%5C+Gymnosperms%5C+chromosomes%EF%BC%8C1%5C+%C2%A0The%5C+Chinese%5C+Seed%5C+Plants%5C+Chromosome%5C+Database%EF%BC%8CThe%5C+frame%5C+of%5C+database%5C+was%5C+constructed%5C+by%5C+Microsoft%5C+Access%5C+2003.%5C+19%5C+items%5C+of%5C+data%5C+were%5C+included%5C+in%2C%5C+they%5C+are%5C%3A%5C+Chinese%5C+and%5C+Latin%5C+names%5C+of%5C+family%2C%5C+genus%5C+and%5C+species%5C%3B%5C+plant%5C+pictures%2C%5C+mitosis%5C+metaphase%5C+and%5C+karyotype%5C+figures%5C%3B%5C+morphological%5C+characteristics%5C+and%5C+distributions%5C+of%5C+the%5C+plant%5C%3B%5C+chromosome%5C+numbers%5C+and%5C+basic%5C+numbers%5C%3B%5C+karyotype%5C+formula%5C%3B%5C+karyotype%5C+description%5C%3B%5C+origin%5C+of%5C+the%5C+plant%5C+material%5C%3B%5C+literature%5C+and%5C+the%5C+source%5C+of%5C+photos.%5C+In%5C+this%5C+database%2C%5C+data%5C+can%5C+be%5C+checked%5C+and%5C+shared%5C+easily%5C+by%5C+extracted%5C+out%5C+in%5C+species%5C+sorted%5C+interface%5C+or%5C+family%5C+sorted%5C+interface.%5C+120%5C+species%5C+in%5C+29%5C+genera%5C+and%5C+10%5C+families%5C+of%5C+Gymnospers%5C+have%5C+been%5C+collected%5C+and%5C+input%5C+to%5C+the%5C+database.%5C+In%5C+Angiosperms%2C%5C+61%5C+species%5C+in%5C+10%5C+genera%5C+of%5C+family%5C+Magnoliaceae%5C+and%5C+80%5C+species%5C+in%5C+3%5C+genera%5C+of%5C+family%5C+Theaceae%5C+have%5C+been%5C+collected%5C+and%5C+input%5C+to%5C+the%5C+database.2%5C+Discussion%5C+on%5C+the%5C+features%5C+of%5C+evolution%5C+and%5C+variation%5C+of%5C+Gymnosperms%5C+chromosomes%EF%BC%8CBy%5C+data%5C+collection%5C+of%5C+the%5C+database%2C%5C+we%5C+analyzed%5C+chromosome%5C+features%5C+of%5C+the%5C+group%5C+Gymnosperm.%5C+Plants%5C+of%5C+Gymnosperm%5C+had%5C+been%5C+through%5C+a%5C+long%5C+historical%5C+evolution%5C+on%5C+earth%2C%5C+fossil%5C+records%5C+of%5C+which%5C+originated%5C+from%5C+the%5C+late%5C+Devonian%5C+period.%5C+Once%5C+an%5C+authoritative%5C+and%5C+major%5C+classification%5C+level%5C+in%5C+the%5C+plant%5C+kingdom%2C%5C+most%5C+Gymnosperms%5C+have%5C+been%5C+extinct%5C+unless%5C+conifers%2C%5C+cycads%2C%5C+Ginkgo%5C+and%5C+Getales.%5C+Three%5C+main%5C+features%5C+of%5C+Gymnosperm%5C+chromosomes%5C+are%5C%3A%5C+relatively%5C+large%5C+chromosome%2C%5C+which%5C+can%5C+be%5C+recognized%5C+from%5C+figures%5C+in%5C+the%5C+database%5C%3B%5C+constant%5C+chromosome%5C+numbers%2C%5C+in%5C+most%5C+families%5C+of%5C+Gymnosperm%5C+the%5C+basic%5C+chromosome%5C+number%5C+keeps%5C+a%5C+certain%5C+value%5C%3B%5C+comparatively%5C+low%5C+variation%2C%5C+karyotype%5C+under%5C+family%5C+level%5C+differs%5C+a%5C+little.%5C+The%5C+variation%5C+of%5C+chromosomes%5C+in%5C+Gymnosperm%5C+is%5C+dominated%5C+by%5C+Robertsonian%5C+changes.%5C+Contrary%5C+to%5C+common%5C+variation%5C+type%5C+in%5C+Angiosperms%2C%5C+the%5C+variation%5C+from%5C+high%5C+unsymmetric%5C+karyotype%5C+to%5C+low%5C+unsymmetric%5C+karyotype%5C+was%5C+found%5C+in%5C+existence%5C+in%5C+Gymnosperm.Part%5C+II%5C%3A%5C+cytology%5C+research%5C+on%5C+some%5C+important%5C+phyto%5C-groups%EF%BC%8C3%5C+Karyomorphology%5C+of%5C+three%5C+species%5C+in%5C+the%5C+order%5C+Huerteales%5C+and%5C+their%5C+phylogenetic%5C+implications%EF%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Light International Fellowship for Chinese Botanists at Missouri Botanical Garden","jscount":"1","jsurl":"/simple-search?field1=all&field=dc.citation.source_filter&advanced=false&query1=GENE%2BORGANIZATION&&fq=dc.project.title_filter%3AGlory%5C+Light%5C+International%5C+Fellowship%5C+for%5C+Chinese%5C+Botanists%5C+at%5C+Missouri%5C+Botanical%5C+Garden"},{"jsname":"How has natural selection determined the evolution of gene regulation by acting on major regulatory factors? This question has been attractive to many evolutionary biologists for a long time. MicroRNAs (miRNAs) are endogenous posttranscriptional repressors and play essential roles in diverse biological processes in plants. To understand how natural selection has targeted on the entire lay of miRNA regulatory modules during flower development, we resequenced 31 miRNA target sites involved in flower development from five rice populations. We found that purifying selection serves as a major evolutionary force to act on the conserved miRNA binding sites, leading to the globally reduced genetic variation in highly conserved miRNA binding sequences within the entire rice samples. Conversely, positive selection allows variations at nonconserved miRNA binding sites and acts on them in a population-specific behaviour. Further analysis revealed that the polymorphisms within target sites may serve as raw materials for diverse functions of miRNAs by means of the destabilization of duplex, abolishment of existing target sites, and creation of novel ones. Together, the above-mentioned results indicate that variations at conserved binding sites are likely deleterious during rice flower development, whereas variants at nonconserved binding sites may be conductive to flower development-related phenotypic diversities and rice population adaption to variable environmental conditions as well. To further assess functional effects and evolutionary significance of variable alleles at the target genes, we reported the detailed characterization of the haplotype and linkage disequilibrium (LD) patterns of the entire target gene (LOC_Os01g18850，SPL 1) and the 1.4 Mb flanking regions in three rice populations, namely japonica, indica and O. rufipogon. The genetic profile of SNPs at target site and its flanking regions revealed high haplotype frequency, low haplotype diversity and strong LD in two cultivatedricepopulations. By contrast, we observed the opposite phenomena in O. rufipogon. Using the long-range haplotype (LRT) test, we found strong evidence of recent positive selection for SNP 3C/T alleles at target site in the combined O. sativa. Comparsion between the two rice subpopulations indicated that the major haplotype mh 2 containing SNP 3C accounts for half of all haplotypes in indica, while mh 3 containing SNP 3T is 91% in japonica. Moreover, the extent of LD is stronger in japonica than that in inidca. These differences suggest that independent evolutionary events may have occurred in target sequences of two cultivated rice populations and stronger positive selection acted on japonica. Next, we examined geographic distribution of polymorphic variants at target sites. We found that the major alleles SNP 3T and tightly linked SNP 4A in japonica appear to be associated with the adaption to the northern climates during rice flower development.","jscount":"1","jsurl":"/simple-search?field1=all&field=dc.citation.source_filter&advanced=false&query1=GENE%2BORGANIZATION&&fq=dc.project.title_filter%3AHow%5C+has%5C+natural%5C+selection%5C+determined%5C+the%5C+evolution%5C+of%5C+gene%5C+regulation%5C+by%5C+acting%5C+on%5C+major%5C+regulatory%5C+factors%5C%3F%5C+This%5C+question%5C+has%5C+been%5C+attractive%5C+to%5C+many%5C+evolutionary%5C+biologists%5C+for%5C+a%5C+long%5C+time.%5C+MicroRNAs%5C+%5C%28miRNAs%5C%29%5C+are%5C+endogenous%5C+posttranscriptional%5C+repressors%5C+and%5C+play%5C+essential%5C+roles%5C+in%5C+diverse%5C+biological%5C+processes%5C+in%5C+plants.%5C+To%5C+understand%5C+how%5C+natural%5C+selection%5C+has%5C+targeted%5C+on%5C+the%5C+entire%5C+lay%5C+of%5C+miRNA%5C+regulatory%5C+modules%5C+during%5C+flower%5C+development%2C%5C+we%5C+resequenced%5C+31%5C+miRNA%5C+target%5C+sites%5C+involved%5C+in%5C+flower%5C+development%5C+from%5C+five%5C+rice%5C+populations.%5C+We%5C+found%5C+that%5C+purifying%5C+selection%5C+serves%5C+as%5C+a%5C+major%5C+evolutionary%5C+force%5C+to%5C+act%5C+on%5C+the%5C+conserved%5C+miRNA%5C+binding%5C+sites%2C%5C+leading%5C+to%5C+the%5C+globally%5C+reduced%5C+genetic%5C+variation%5C+in%5C+highly%5C+conserved%5C+miRNA%5C+binding%5C+sequences%5C+within%5C+the%5C+entire%5C+rice%5C+samples.%5C+Conversely%2C%5C+positive%5C+selection%5C+allows%5C+variations%5C+at%5C+nonconserved%5C+miRNA%5C+binding%5C+sites%5C+and%5C+acts%5C+on%5C+them%5C+in%5C+a%5C+population%5C-specific%5C+behaviour.%5C+Further%5C+analysis%5C+revealed%5C+that%5C+the%5C+polymorphisms%5C+within%5C+target%5C+sites%5C+may%5C+serve%5C+as%5C+raw%5C+materials%5C+for%5C+diverse%5C+functions%5C+of%5C+miRNAs%5C+by%5C+means%5C+of%5C+the%5C+destabilization%5C+of%5C+duplex%2C%5C+abolishment%5C+of%5C+existing%5C+target%5C+sites%2C%5C+and%5C+creation%5C+of%5C+novel%5C+ones.%5C+Together%2C%5C+the%5C+above%5C-mentioned%5C+results%5C+indicate%5C+that%5C+variations%5C+at%5C+conserved%5C+binding%5C+sites%5C+are%5C+likely%5C+deleterious%5C+during%5C+rice%5C+flower%5C+development%2C%5C+whereas%5C+variants%5C+at%5C+nonconserved%5C+binding%5C+sites%5C+may%5C+be%5C+conductive%5C+to%5C+flower%5C+development%5C-related%5C+phenotypic%5C+diversities%5C+and%5C+rice%5C+population%5C+adaption%5C+to%5C+variable%5C+environmental%5C+conditions%5C+as%5C+well.%5C+To%5C+further%5C+assess%5C+functional%5C+effects%5C+and%5C+evolutionary%5C+significance%5C+of%5C+variable%5C+alleles%5C+at%5C+the%5C+target%5C+genes%2C%5C+we%5C+reported%5C+the%5C+detailed%5C+characterization%5C+of%5C+the%5C+haplotype%5C+and%5C+linkage%5C+disequilibrium%5C+%5C%28LD%5C%29%5C+patterns%5C+of%5C+the%5C+entire%5C+target%5C+gene%5C+%5C%28LOC_Os01g18850%EF%BC%8CSPL%5C+1%5C%29%5C+and%5C+the%5C+1.4%5C+Mb%5C+flanking%5C+regions%5C+in%5C+three%5C+rice%5C+populations%2C%5C+namely%5C+japonica%2C%5C+indica%5C+and%5C+O.%5C+rufipogon.%5C+The%5C+genetic%5C+profile%5C+of%5C+SNPs%5C+at%5C+target%5C+site%5C+and%5C+its%5C+flanking%5C+regions%5C+revealed%5C+high%5C+haplotype%5C+frequency%2C%5C+low%5C+haplotype%5C+diversity%5C+and%5C+strong%5C+LD%5C+in%5C+two%5C+cultivatedricepopulations.%5C+By%5C+contrast%2C%5C+we%5C+observed%5C+the%5C+opposite%5C+phenomena%5C+in%5C+O.%5C+rufipogon.%5C+Using%5C+the%5C+long%5C-range%5C+haplotype%5C+%5C%28LRT%5C%29%5C+test%2C%5C+we%5C+found%5C+strong%5C+evidence%5C+of%5C+recent%5C+positive%5C+selection%5C+for%5C+SNP%5C+3C%5C%2FT%5C+alleles%5C+at%5C+target%5C+site%5C+in%5C+the%5C+combined%5C+O.%5C+sativa.%5C+Comparsion%5C+between%5C+the%5C+two%5C+rice%5C+subpopulations%5C+indicated%5C+that%5C+the%5C+major%5C+haplotype%5C+mh%5C+2%5C+containing%5C+SNP%5C+3C%5C+accounts%5C+for%5C+half%5C+of%5C+all%5C+haplotypes%5C+in%5C+indica%2C%5C+while%5C+mh%5C+3%5C+containing%5C+SNP%5C+3T%5C+is%5C+91%25%5C+in%5C+japonica.%5C+Moreover%2C%5C+the%5C+extent%5C+of%5C+LD%5C+is%5C+stronger%5C+in%5C+japonica%5C+than%5C+that%5C+in%5C+inidca.%5C+These%5C+differences%5C+suggest%5C+that%5C+independent%5C+evolutionary%5C+events%5C+may%5C+have%5C+occurred%5C+in%5C+target%5C+sequences%5C+of%5C+two%5C+cultivated%5C+rice%5C+populations%5C+and%5C+stronger%5C+positive%5C+selection%5C+acted%5C+on%5C+japonica.%5C+Next%2C%5C+we%5C+examined%5C+geographic%5C+distribution%5C+of%5C+polymorphic%5C+variants%5C+at%5C+target%5C+sites.%5C+We%5C+found%5C+that%5C+the%5C+major%5C+alleles%5C+SNP%5C+3T%5C+and%5C+tightly%5C+linked%5C+SNP%5C+4A%5C+in%5C+japonica%5C+appear%5C+to%5C+be%5C+associated%5C+with%5C+the%5C+adaption%5C+to%5C+the%5C+northern%5C+climates%5C+during%5C+rice%5C+flower%5C+development."},{"jsname":"Interdisciplinary Research Project of Kunming Institute of Botany[KIB2017003]","jscount":"1","jsurl":"/simple-search?field1=all&field=dc.citation.source_filter&advanced=false&query1=GENE%2BORGANIZATION&&fq=dc.project.title_filter%3AInterdisciplinary%5C+Research%5C+Project%5C+of%5C+Kunming%5C+Institute%5C+of%5C+Botany%5C%5BKIB2017003%5C%5D"},{"jsname":"Kunming Institute of Botany, Chinese Academy of Sciences","jscount":"1","jsurl":"/simple-search?field1=all&field=dc.citation.source_filter&advanced=false&query1=GENE%2BORGANIZATION&&fq=dc.project.title_filter%3AKunming%5C+Institute%5C+of%5C+Botany%2C%5C+Chinese%5C+Academy%5C+of%5C+Sciences"},{"jsname":"Large-scale Scientific Facilities of the Chinese Academy of Sciences[2017-LSFGBOWS-01]","jscount":"1","jsurl":"/simple-search?field1=all&field=dc.citation.source_filter&advanced=false&query1=GENE%2BORGANIZATION&&fq=dc.project.title_filter%3ALarge%5C-scale%5C+Scientific%5C+Facilities%5C+of%5C+the%5C+Chinese%5C+Academy%5C+of%5C+Sciences%5C%5B2017%5C-LSFGBOWS%5C-01%5C%5D"},{"jsname":"Light of the West Talent Cultivation Program of Chinese Academy of Sciences[201684]","jscount":"1","jsurl":"/simple-search?field1=all&field=dc.citation.source_filter&advanced=false&query1=GENE%2BORGANIZATION&&fq=dc.project.title_filter%3ALight%5C+of%5C+the%5C+West%5C+Talent%5C+Cultivation%5C+Program%5C+of%5C+Chinese%5C+Academy%5C+of%5C+Sciences%5C%5B201684%5C%5D"},{"jsname":"Low temperature stress is one of the main environmental factors which limit plant growth, development and distribution. The physiological, biochemical and molecular mechanisms research on plant response to low temperature injury can provide theoretical basis for improving plant tolerance to low temperature，and benefit agricultural production practices. The membrane is a major injury site induced by low temperature. Previous studies have shown that membrane lipids have undergone great changes in low-temperature treatments like cold acclimation, freezing and thawing.This study used ESI / MS-MS and TLC technologies to detecte the changes of membrane lipid species in the process of low-temperature injury and injury recovery in Arabidopsis thaliana, Brassica napus, Oryza sativa (rice) and Cucumis satirus (cucumber). Arabidopsis and Brassica napus have resistance to subzero low temperature to some extent, whereas rice and cucumber are sensitive to unfreezing low temperature.When Arabidopsisexperienced reversible low temperature stress, absolute concentration of the membrane lipid species changed，while this change could back to normal after temperature returned to normal. When the plant could not be restored through the low-temperature stress and died, membrane lipid species except PA and lysophospholipids had undergone a large-scale degradation orderly, which occurred most violently in the process of thawing. PA increased significantly during thawing, and degraded during cold de-acclimation, but still significantly higher than normal level. At the same time, some new type of membrane lipid species appeared. The changing pattern of two types of glycolipids were different: MGDG changed drastically while DGDG changed moderately. On the contrast of different Arabidopsis genotypes underwent low temperature stress found that there were no significant difference between mutant-type and wild-type, indicating PLDα1 and PLDδ play a small role in low temperature process of degradation of membrane lipid induced by irreversible injury. Many phospholipase involved in lipid degradation. By the research on relative content of various lipids found that some lipids decreased in absolute concentration while increased in the relative concentration indicating the degradation order and rate of different lipids was different. Under the reversible low temperature stress, the double bond index (DBI) of membrane lipid was unchanged, while that changed continuously under the irreversible low temperature stress. Specific analysis on changes in DBI of every lipid class revealed that changes were not obvious, which indicated that under irreversible low-temperature stress, the decrease of relative concentration of some membrane lipids like MGDG which had high DBI induced the DBI of total membrane lipid to decline.There was similar discipline in Brassica napus. When rice and cucumber experienced reversible and irreversible unfreezing low-temperature stress, PA also increased and other lipids decreased, indicating low-temperature-induced membrane disintegration had little to do with intracellular ice.In short, when plant experienced reversible low-temperature injury, lipid species could regulate the ratio of each component to ensure the survival of plants. When the temperature returned to normal, the membrane lipids had also been restored. When the plants suffered irreversible damage at low temperature, the membrane lipid molecules would degrade orderly and severely. Various types of membrane lipid molecules might be first degraded to PA, then PA degraded, so PA would first significantly increase, and then decreased. The knowledge on discipline of plants response to low temperature stress on the necrotic process can provide a theoretical basis for us to avoid the plants necrosis induced by cold and other stress-induced.","jscount":"1","jsurl":"/simple-search?field1=all&field=dc.citation.source_filter&advanced=false&query1=GENE%2BORGANIZATION&&fq=dc.project.title_filter%3ALow%5C+temperature%5C+stress%5C+is%5C+one%5C+of%5C+the%5C+main%5C+environmental%5C+factors%5C+which%5C+limit%5C+plant%5C+growth%2C%5C+development%5C+and%5C+distribution.%5C+The%5C+physiological%2C%5C+biochemical%5C+and%5C+molecular%5C+mechanisms%5C+research%5C+on%5C+plant%5C+response%5C+to%5C+low%5C+temperature%5C+injury%5C+can%5C+provide%5C+theoretical%5C+basis%5C+for%5C+improving%5C+plant%5C+tolerance%5C+to%5C+low%5C+temperature%EF%BC%8Cand%5C+benefit%5C+agricultural%5C+production%5C+practices.%5C+The%5C+membrane%5C+is%5C+a%5C+major%5C+injury%5C+site%5C+induced%5C+by%5C+low%5C+temperature.%5C+Previous%5C+studies%5C+have%5C+shown%5C+that%5C+membrane%5C+lipids%5C+have%5C+undergone%5C+great%5C+changes%5C+in%5C+low%5C-temperature%5C+treatments%5C+like%5C+cold%5C+acclimation%2C%5C+freezing%5C+and%5C+thawing.This%5C+study%5C+used%5C+ESI%5C+%5C%2F%5C+MS%5C-MS%5C+and%5C+TLC%5C+technologies%5C+to%5C+detecte%5C+the%5C+changes%5C+of%5C+membrane%5C+lipid%5C+species%5C+in%5C+the%5C+process%5C+of%5C+low%5C-temperature%5C+injury%5C+and%5C+injury%5C+recovery%5C+in%5C+Arabidopsis%5C+thaliana%2C%5C+Brassica%5C+napus%2C%5C+Oryza%5C+sativa%5C+%5C%28rice%5C%29%5C+and%5C+Cucumis%5C+satirus%5C+%5C%28cucumber%5C%29.%5C+Arabidopsis%5C+and%5C+Brassica%5C+napus%5C+have%5C+resistance%5C+to%5C+subzero%5C+low%5C+temperature%5C+to%5C+some%5C+extent%2C%5C+whereas%5C+rice%5C+and%5C+cucumber%5C+are%5C+sensitive%5C+to%5C+unfreezing%5C+low%5C+temperature.When%5C+Arabidopsisexperienced%5C+reversible%5C+low%5C+temperature%5C+stress%2C%5C+absolute%5C+concentration%5C+of%5C+the%5C+membrane%5C+lipid%5C+species%5C+changed%EF%BC%8Cwhile%5C+this%5C+change%5C+could%5C+back%5C+to%5C+normal%5C+after%5C+temperature%5C+returned%5C+to%5C+normal.%5C+When%5C+the%5C+plant%5C+could%5C+not%5C+be%5C+restored%5C+through%5C+the%5C+low%5C-temperature%5C+stress%5C+and%5C+died%2C%5C+membrane%5C+lipid%5C+species%5C+except%5C+PA%5C+and%5C+lysophospholipids%5C+had%5C+undergone%5C+a%5C+large%5C-scale%5C+degradation%5C+orderly%2C%5C+which%5C+occurred%5C+most%5C+violently%5C+in%5C+the%5C+process%5C+of%5C+thawing.%5C+PA%5C+increased%5C+significantly%5C+during%5C+thawing%2C%5C+and%5C+degraded%5C+during%5C+cold%5C+de%5C-acclimation%2C%5C+but%5C+still%5C+significantly%5C+higher%5C+than%5C+normal%5C+level.%5C+At%5C+the%5C+same%5C+time%2C%5C+some%5C+new%5C+type%5C+of%5C+membrane%5C+lipid%5C+species%5C+appeared.%5C+The%5C+changing%5C+pattern%5C+of%5C+two%5C+types%5C+of%5C+glycolipids%5C+were%5C+different%5C%3A%5C+MGDG%5C+changed%5C+drastically%5C+while%5C+DGDG%5C+changed%5C+moderately.%5C+On%5C+the%5C+contrast%5C+of%5C+different%5C+Arabidopsis%5C+genotypes%5C+underwent%5C+low%5C+temperature%5C+stress%5C+found%5C+that%5C+there%5C+were%5C+no%5C+significant%5C+difference%5C+between%5C+mutant%5C-type%5C+and%5C+wild%5C-type%2C%5C+indicating%5C+PLD%CE%B11%5C+and%5C+PLD%CE%B4%5C+play%5C+a%5C+small%5C+role%5C+in%5C+low%5C+temperature%5C+process%5C+of%5C+degradation%5C+of%5C+membrane%5C+lipid%5C+induced%5C+by%5C+irreversible%5C+injury.%5C+Many%5C+phospholipase%5C+involved%5C+in%5C+lipid%5C+degradation.%5C+By%5C+the%5C+research%5C+on%5C+relative%5C+content%5C+of%5C+various%5C+lipids%5C+found%5C+that%5C+some%5C+lipids%5C+decreased%5C+in%5C+absolute%5C+concentration%5C+while%5C+increased%5C+in%5C+the%5C+relative%5C+concentration%5C+indicating%5C+the%5C+degradation%5C+order%5C+and%5C+rate%5C+of%5C+different%5C+lipids%5C+was%5C+different.%5C+Under%5C+the%5C+reversible%5C+low%5C+temperature%5C+stress%2C%5C+the%5C+double%5C+bond%5C+index%5C+%5C%28DBI%5C%29%5C+of%5C+membrane%5C+lipid%5C+was%5C+unchanged%2C%5C+while%5C+that%5C+changed%5C+continuously%5C+under%5C+the%5C+irreversible%5C+low%5C+temperature%5C+stress.%5C+Specific%5C+analysis%5C+on%5C+changes%5C+in%5C+DBI%5C+of%5C+every%5C+lipid%5C+class%5C+revealed%5C+that%5C+changes%5C+were%5C+not%5C+obvious%2C%5C+which%5C+indicated%5C+that%5C+under%5C+irreversible%5C+low%5C-temperature%5C+stress%2C%5C+the%5C+decrease%5C+of%5C+relative%5C+concentration%5C+of%5C+some%5C+membrane%5C+lipids%5C+like%5C+MGDG%5C+which%5C+had%5C+high%5C+DBI%5C+induced%5C+the%5C+DBI%5C+of%5C+total%5C+membrane%5C+lipid%5C+to%5C+decline.There%5C+was%5C+similar%5C+discipline%5C+in%5C+Brassica%5C+napus.%5C+When%5C+rice%5C+and%5C+cucumber%5C+experienced%5C+reversible%5C+and%5C+irreversible%5C+unfreezing%5C+low%5C-temperature%5C+stress%2C%5C+PA%5C+also%5C+increased%5C+and%5C+other%5C+lipids%5C+decreased%2C%5C+indicating%5C+low%5C-temperature%5C-induced%5C+membrane%5C+disintegration%5C+had%5C+little%5C+to%5C+do%5C+with%5C+intracellular%5C+ice.In%5C+short%2C%5C+when%5C+plant%5C+experienced%5C+reversible%5C+low%5C-temperature%5C+injury%2C%5C+lipid%5C+species%5C+could%5C+regulate%5C+the%5C+ratio%5C+of%5C+each%5C+component%5C+to%5C+ensure%5C+the%5C+survival%5C+of%5C+plants.%5C+When%5C+the%5C+temperature%5C+returned%5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of Science and Technology, Taiwan[106-2311-B-001-005]","jscount":"1","jsurl":"/simple-search?field1=all&field=dc.citation.source_filter&advanced=false&query1=GENE%2BORGANIZATION&&fq=dc.project.title_filter%3AMinistry%5C+of%5C+Science%5C+and%5C+Technology%2C%5C+Taiwan%5C%5B106%5C-2311%5C-B%5C-001%5C-005%5C%5D"},{"jsname":"lastIndexed","jscount":"2024-04-15"}],"资助项目","dc.project.title_filter")'>
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