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资助项目
0.05). For some populations, germination capacity in 12-h photoperiod was significantly higher than that in completed darkness（W-FD: P < 0.01, W-JD: P < 0.05）.Genetic variation within and among six populations was assessed using AFLP markers. Genetic diversity was higher at species level (PPL = 69.19%, HE = 0.221) than at population level (PPL = 26.22%, HE = 0.095, Is =0.140), and populations in southeast Yunnan were strongly differentiated from those in southwest Yunnan (Nei’s GST = 0.575; FST = 0.655). UPGMA analysis demonstrated a clear genetic division between the two populations from DeHong (SW Yunnan; D-JD and D-HG) and the four from WenShan (SE Yunnan; W-FD, W-LH, W-ML, and W-MG). Within-population genetic variation was significantly correlated with population isolation (r(PPL) = -0.94, P = 0.006; r(HE) = -0.85, P = 0.032; r(Is) = -0.87, P = 0.025), but not with population size (r(PPL) = 0.63, P = 0.178; r(HE) = 0.54, P = 0.268; r(Is) = 0.56, P = 0.249).","jscount":"1","jsurl":"/simple-search?field1=all&field=dc.citation.source_filter&advanced=false&query1=Ethiopia&&fq=dc.project.title_filter%3ACraigia%5C+yunnanensis%5C+W.%5C+W.%5C+Smith%5C+%5C%26%5C+W.%5C+E.%5C+Evans%5C+%5C%28Tiliaceae%5C%29%5C+is%5C+an%5C+endangered%5C+deciduous%5C+tree%5C+species%5C+which%5C+has%5C+high%5C+scientific%5C+and%5C+economic%5C+value.%5C+C.%5C+yunnanensis%5C+is%5C+seriously%5C+threatened%5C+and%5C+has%5C+been%5C+pushed%5C+to%5C+the%5C+verge%5C+of%5C+extinction%5C+due%5C+to%5C+vegetation%5C+destruction%5C+in%5C+China%5C+and%5C+consequent%5C+contraction%5C+of%5C+its%5C+distribution.%5C+Hence%2C%5C+it%5C+was%5C+listed%5C+as%5C+a%5C+nationally%5C+rare%5C+and%5C+endangered%5C+plant%5C+in%5C+1999%5C+and%5C+has%5C+also%5C+been%5C+proposed%5C+as%5C+a%5C+second%5C-ranked%5C+plant%5C+for%5C+national%5C+protection%5C+in%5C+China%5C+and%5C+included%5C+in%5C+IUCN%5C+red%5C+list.%5C+As%5C+a%5C+scientifically%5C+important%5C+and%5C+valued%5C+tree%5C+species%5C+with%5C+endangered%5C+status%2C%5C+the%5C+wild%5C+populations%5C+of%5C+C.%5C+yunnanensis%5C+therefore%5C+represent%5C+is%5C+a%5C+genetic%5C+resource%5C+that%5C+must%5C+be%5C+conserved.%5C+To%5C+provide%5C+basic%5C+information%5C+for%5C+its%5C+conservation%2C%5C+the%5C+population%5C+dynamics%5C+and%5C+population%5C+size%5C+structures%2C%5C+pollination%5C+biology%5C+and%5C+breeding%5C+system%2C%5C+eleven%5C+fitness%5C-related%5C+characters%5C+and%5C+the%5C+genetic%5C+variability%5C+based%5C+on%5C+AFLP%5C+were%5C+comprehensively%5C+studied.%5C+The%5C+main%5C+results%5C+are%5C+summarized%5C+as%5C+follows%5C%3A%5C+A%5C+total%5C+of%5C+six%5C+wild%5C+populations%5C+of%5C+C.%5C+yunnanensis%5C+were%5C+found%5C+in%5C+two%5C+disjunct%5C+regions%5C+of%5C+Yunnan%2C%5C+i.e.%5C+WenShan%5C+%5C%28SE%5C+Yunnan%5C%29%5C+and%5C+DeHong%5C+%5C%28SW%5C+Yunnan%5C%29%2C%5C+from%5C+2005%5C+to%5C+2007.%5C+Additionally%2C%5C+in%5C+all%5C+but%5C+one%5C+of%5C+the%5C+populations%5C+we%5C+detected%2C%5C+mature%5C+trees%5C+were%5C+felled%5C+between%5C+2005%5C+and%5C+2007%2C%5C+so%5C+destruction%5C+of%5C+most%5C+of%5C+these%5C+populations%5C+is%5C+ongoing.%5C+Across%5C+the%5C+six%5C+populations%5C+of%5C+extant%5C+C.%5C+yunnanensis%5C+found%5C+during%5C+our%5C+study%2C%5C+the%5C+total%5C+number%5C+of%5C+mature%5C+%5C%28reproductive%5C%29%5C+individuals%5C+detected%5C+was%5C+584%5C+in%5C+2007%EF%BC%8Cplus%5C+larger%5C+numbers%5C+of%5C+seedling%5C+and%5C+resprouts%5C+from%5C+cut%5C+trunks.%5C+The%5C+result%5C+of%5C+surveying%5C+Population%5C+structure%5C+showed%5C+that%5C+there%5C+are%5C+two%5C+regeneration%5C+types%5C+which%5C+are%5C+seedlings%5C+and%5C+sprouts.%5C+Seedlings%5C+occurred%5C+abundantly%5C+in%5C+gaps%5C+or%5C+open%5C+areas%5C+and%5C+the%5C+size%5C+class%5C+frequency%5C+distributions%5C+were%5C+often%5C+discontinuous%2C%5C+and%5C+the%5C+same%5C+general%5C+pattern%5C+occurred%5C+in%5C+all%5C+the%5C+investigated%5C+populations%5C+for%5C+juveniles%5C+and%5C+adults.%5C+The%5C+numbers%5C+of%5C+seed%5C-origin%5C+individuals%5C+did%5C+however%5C+decline%5C+sharply%5C+with%5C+increasing%5C+size%2C%5C+indicating%5C+a%5C+high%5C+mortality%5C+rate%5C+going%5C+from%5C+seedling%5C+to%5C+sapling%5C+stage%5C+may%5C+be%5C+a%5C+problem%5C+for%5C+this%5C+species.%5C+Additionally%2C%5C+the%5C+cash%5C+crop%5C+cultivation%5C+and%5C+logging%5C+seriously%5C+threaten%5C+the%5C+survival%5C+of%5C+the%5C+species.%5C+We%5C+conducted%5C+field%5C+observations%5C+and%5C+artificial%5C+pollination%5C+experiments%5C+on%5C+the%5C+floral%5C+biology%2C%5C+pollination%5C+process%5C+and%5C+breeding%5C+system%5C+of%5C+Craigia%5C+yunnanensis%5C+in%5C+Fadou%2C%5C+Xichou%5C+county%5C+of%5C+Yunnan%5C+province.%5C+The%5C+lifespan%5C+of%5C+a%5C+single%5C+hermaphrodite%5C+flower%5C+is%5C+approximately%5C+3%5C-4%5C+days.%5C+A%5C+cyme%5C+has%5C+2%5C-9%5C+flowered.%5C+The%5C+flowering%5C+period%5C+of%5C+an%5C+inflorescence%5C+is%5C+usually%5C+5%5C-14%5C+days.%5C+The%5C+flowers%5C+of%5C+C.%5C+yunnanensis%5C+were%5C+protandrous.%5C+The%5C+stamens%5C+were%5C+within%5C+petal%5C-like%5C+staminodes%5C+in%5C+the%5C+opening%5C+flowers%5C+until%5C+the%5C+flower%5C+withered.%5C+Without%5C+touchment%2C%5C+the%5C+bractlike%5C+staminodes%5C+can%E2%80%99t%5C+open.%5C+Self%5C-pollination%5C+was%5C+partially%5C+avoided%5C+by%5C+temporal%5C+and%5C+spatial%5C+isolation%5C+of%5C+male%5C+and%5C+female%5C+organs%5C+within%5C+the%5C+same%5C+flower.%5C+However%2C%5C+autogamous%5C+and%5C+geitonogamous%5C+pollination%5C+is%5C+unavoidable%5C+because%5C+of%5C+the%5C+large%5C+number%5C+of%5C+flowers%5C+on%5C+a%5C+single%5C+tree%5C+and%5C+the%5C+action%5C+of%5C+pollinators.%5C+The%5C+values%5C+of%5C+both%5C+OCI%5C+%5C%28%E2%89%A54%5C%29%5C+and%5C+P%5C%2FO%5C+%5C%281381%5C%29%5C+and%5C+the%5C+results%5C+of%5C+bagging%5C+tests%5C+indicated%5C+there%5C+was%5C+no%5C+apomixes%5C+in%5C+C.%5C+yunnanensis%5C+and%5C+the%5C+breeding%5C+system%5C+of%5C+the%5C+species%5C+was%5C+outcrossing%5C+with%5C+partial%5C+self%5C-compatibility%5C+and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MSCA individual fellowship[705432]","jscount":"1","jsurl":"/simple-search?field1=all&field=dc.citation.source_filter&advanced=false&query1=Ethiopia&&fq=dc.project.title_filter%3AEU%5C+MSCA%5C+individual%5C+fellowship%5C%5B705432%5C%5D"},{"jsname":"EU MSCA individual fellowship[750252]","jscount":"1","jsurl":"/simple-search?field1=all&field=dc.citation.source_filter&advanced=false&query1=Ethiopia&&fq=dc.project.title_filter%3AEU%5C+MSCA%5C+individual%5C+fellowship%5C%5B750252%5C%5D"},{"jsname":"European Research Council through the Advanced Grant Project TREEPEACE[FP7-339728]","jscount":"1","jsurl":"/simple-search?field1=all&field=dc.citation.source_filter&advanced=false&query1=Ethiopia&&fq=dc.project.title_filter%3AEuropean%5C+Research%5C+Council%5C+through%5C+the%5C+Advanced%5C+Grant%5C+Project%5C+TREEPEACE%5C%5BFP7%5C-339728%5C%5D"},{"jsname":"Ministry of Science and Technology of the People''s Republic of China[2012FY110300]","jscount":"1","jsurl":"/simple-search?field1=all&field=dc.citation.source_filter&advanced=false&query1=Ethiopia&&fq=dc.project.title_filter%3AMinistry%5C+of%5C+Science%5C+and%5C+Technology%5C+of%5C+the%5C+People%27%27s%5C+Republic%5C+of%5C+China%5C%5B2012FY110300%5C%5D"},{"jsname":"NASA[NNX12AK56G]","jscount":"1","jsurl":"/simple-search?field1=all&field=dc.citation.source_filter&advanced=false&query1=Ethiopia&&fq=dc.project.title_filter%3ANASA%5C%5BNNX12AK56G%5C%5D"},{"jsname":"National Natural Science Foundation of China[31400286]","jscount":"1","jsurl":"/simple-search?field1=all&field=dc.citation.source_filter&advanced=false&query1=Ethiopia&&fq=dc.project.title_filter%3ANational%5C+Natural%5C+Science%5C+Foundation%5C+of%5C+China%5C%5B31400286%5C%5D"},{"jsname":"National Natural Science Foundation of China[31770368]","jscount":"1","jsurl":"/simple-search?field1=all&field=dc.citation.source_filter&advanced=false&query1=Ethiopia&&fq=dc.project.title_filter%3ANational%5C+Natural%5C+Science%5C+Foundation%5C+of%5C+China%5C%5B31770368%5C%5D"},{"jsname":"National Natural Science Foundation of China[41661144001]","jscount":"1","jsurl":"/simple-search?field1=all&field=dc.citation.source_filter&advanced=false&query1=Ethiopia&&fq=dc.project.title_filter%3ANational%5C+Natural%5C+Science%5C+Foundation%5C+of%5C+China%5C%5B41661144001%5C%5D"},{"jsname":"Russian Science Foundation[16-14-10208]","jscount":"1","jsurl":"/simple-search?field1=all&field=dc.citation.source_filter&advanced=false&query1=Ethiopia&&fq=dc.project.title_filter%3ARussian%5C+Science%5C+Foundation%5C%5B16%5C-14%5C-10208%5C%5D"},{"jsname":"Southeast Asia Biodiversity Research Institute, Chinese Academy of Sciences[2015CASEABRIRG001]","jscount":"1","jsurl":"/simple-search?field1=all&field=dc.citation.source_filter&advanced=false&query1=Ethiopia&&fq=dc.project.title_filter%3ASoutheast%5C+Asia%5C+Biodiversity%5C+Research%5C+Institute%2C%5C+Chinese%5C+Academy%5C+of%5C+Sciences%5C%5B2015CASEABRIRG001%5C%5D"},{"jsname":"This dissertation is composed of four chapters, in which the chemical constituents of three medicinal plants, Abrus precatorius L., Echinops ritro L., and Saussurea laniceps Hand-Mazz. have been phytochemically studied. 150 compounds, including 10 new ones, were isolated from above mentioned three species. Their structures were established on the basis of extensive spectroscopic methods in conjunction with single-crystal X-ray diffraction analysis and computational methods. Chapter 4 is a review about the chemical constituentsand bioactivities from the plants genus Echinops of the family Compositae. These compounds include monoterpenes, sesquiterpenes (including guaiane and eudesmane mainly), triterpenoids, alkaloids, thiophenes, coumarins, steroids, lignans, flavones and its glycosides. A series of guaiane and eudesmane type sesquiterpenes have been isolated from Echinops ritro L., which is the first time in Echinops species. 21 substituted thiophenes (including 4 new ones) have been isolated from it. Some of them exhibited significant anti-microbial activity. As well as, one novel sulf-polyacetylene ester was isolated from Echinops ritro L.. The computational prediction of optical rotation values and 13C NMR chemical shift was attempted to elucidate structure of natural products. A series of sesquiterpenes have been isolated from Saussurea laniceps Hand-Mazz.","jscount":"1","jsurl":"/simple-search?field1=all&field=dc.citation.source_filter&advanced=false&query1=Ethiopia&&fq=dc.project.title_filter%3AThis%5C+dissertation%5C+is%5C+composed%5C+of%5C+four%5C+chapters%2C%5C+in%5C+which%5C+the%5C+chemical%5C+constituents%5C+of%5C+three%5C+medicinal%5C+plants%2C%5C+Abrus%5C+precatorius%5C+L.%2C%5C+Echinops%5C+ritro%5C+L.%2C%5C+and%5C+Saussurea%5C+laniceps%5C+Hand%5C-Mazz.%5C+have%5C+been%5C+phytochemically%5C+studied.%5C+150%5C+compounds%2C%5C+including%5C+10%5C+new%5C+ones%2C%5C+were%5C+isolated%5C+from%5C+above%5C+mentioned%5C+three%5C+species.%5C+Their%5C+structures%5C+were%5C+established%5C+on%5C+the%5C+basis%5C+of%5C+extensive%5C+spectroscopic%5C+methods%5C+in%5C+conjunction%5C+with%5C+single%5C-crystal%5C+X%5C-ray%5C+diffraction%5C+analysis%5C+and%5C+computational%5C+methods.%5C+Chapter%5C+4%5C+is%5C+a%5C+review%5C+about%5C+the%5C+chemical%5C+constituentsand%5C+bioactivities%5C+from%5C+the%5C+plants%5C+genus%5C+Echinops%5C+of%5C+the%5C+family%5C+Compositae.%5C+These%5C+compounds%5C+include%5C+monoterpenes%2C%5C+sesquiterpenes%5C+%5C%28including%5C+guaiane%5C+and%5C+eudesmane%5C+mainly%5C%29%2C%5C+triterpenoids%2C%5C+alkaloids%2C%5C+thiophenes%2C%5C+coumarins%2C%5C+steroids%2C%5C+lignans%2C%5C+flavones%5C+and%5C+its%5C+glycosides.%5C+A%5C+series%5C+of%5C+guaiane%5C+and%5C+eudesmane%5C+type%5C+sesquiterpenes%5C+have%5C+been%5C+isolated%5C+from%5C+Echinops%5C+ritro%5C+L.%2C%5C+which%5C+is%5C+the%5C+first%5C+time%5C+in%5C+Echinops%5C+species.%5C+21%5C+substituted%5C+thiophenes%5C+%5C%28including%5C+4%5C+new%5C+ones%5C%29%5C+have%5C+been%5C+isolated%5C+from%5C+it.%5C+Some%5C+of%5C+them%5C+exhibited%5C+significant%5C+anti%5C-microbial%5C+activity.%5C+As%5C+well%5C+as%2C%5C+one%5C+novel%5C+sulf%5C-polyacetylene%5C+ester%5C+was%5C+isolated%5C+from%5C+Echinops%5C+ritro%5C+L..%5C+The%5C+computational%5C+prediction%5C+of%5C+optical%5C+rotation%5C+values%5C+and%5C+13C%5C+NMR%5C+chemical%5C+shift%5C+was%5C+attempted%5C+to%5C+elucidate%5C+structure%5C+of%5C+natural%5C+products.%5C+A%5C+series%5C+of%5C+sesquiterpenes%5C+have%5C+been%5C+isolated%5C+from%5C+Saussurea%5C+laniceps%5C+Hand%5C-Mazz."},{"jsname":"This thesis includes two parts: the investigation of medicinal plants in the communities of De''ang people, and the phytochemical studies on three medicinal plants used by the De''ang people traditionally. The De''ang people is an old indigenous minority in China. Most De''ang people live in the Southwest of Yunan Province. They have accumulated abundant traditional knowledge about utilization of medicinal plants in their long-time live and produce. However, without written script of their own and with the influence of alien cultures, their indigenous knowledge is facing the risk of being assimilated or extinct. We carried out an ethnoparmacological investigation of medicinal plants in De''ang communities. It has been found that 92 species were highly valuable for medicinal uses including heat-clearing and detoxication, anti-inflammatory pain, bone fracture and injury, relieving rigidity of muscles and promoting blood circulation, snake bite and so on. Of the 92 plants recorded, 23 species were newly recorded for the De''ang people, and new medicinal properties of one plant species (Tacca chantrieri) was the first record too. In order to know well of the medicinal properties of traditional medicinal plants used by the De''ang people, we selected three plants (Piper boehmeriaefolium, Remusatia vivipara and Pilea cavaleriei subsp. crenata) for the chemical constituents research. Forty-six compounds including 13 new ones were isolated from the above mentioned folk medicinal plants. The types of these compounds involved amide alkaloids, lignans, phenylpropanoids, terpenoids, flavanoids and so on. In addition, some of the isolates were tested for their bioactivities. Thirty compounds, including 8 new ones, were isolated from the whole plant of Piper boehmeriaefolium (Piperaceae). The compounds from the plant mainly belonged to the alkaloids of amides. A new phenylpropanoid glucoside, caffeyl alcohol-3-O-b-d-glucopyranoside, together with nine known compounds, was isolated from the dry corms of Remusatia vivipara (Araceae). The structure of the new compound was determined by the spectroscopic method and acidic hydrolysis. The compounds from the plant mainly belonged to phenlpropanoids, neolignans, steroid saponin, triterpenoid saponin and amides. All compounds were isolated from the genus Remusatia for the first time. Three new humulane-type sesquiterpenes, and a new copaborneol derivative, , together with two known compounds have been isolated from the whole plant of Pilea cavaleriei subsp. crenata (Urticaceae). All compounds were isolated from the genus Pilea for the first time.","jscount":"1","jsurl":"/simple-search?field1=all&field=dc.citation.source_filter&advanced=false&query1=Ethiopia&&fq=dc.project.title_filter%3AThis%5C+thesis%5C+includes%5C+two%5C+parts%5C%3A%5C+the%5C+investigation%5C+of%5C+medicinal%5C+plants%5C+in%5C+the%5C+communities%5C+of%5C+De%27%27ang%5C+people%2C%5C+and%5C+the%5C+phytochemical%5C+studies%5C+on%5C+three%5C+medicinal%5C+plants%5C+used%5C+by%5C+the%5C+De%27%27ang%5C+people%5C+traditionally.%5C+The%5C+De%27%27ang%5C+people%5C+is%5C+an%5C+old%5C+indigenous%5C+minority%5C+in%5C+China.%5C+Most%5C+De%27%27ang%5C+people%5C+live%5C+in%5C+the%5C+Southwest%5C+of%5C+Yunan%5C+Province.%5C+They%5C+have%5C+accumulated%5C+abundant%5C+traditional%5C+knowledge%5C+about%5C+utilization%5C+of%5C+medicinal%5C+plants%5C+in%5C+their%5C+long%5C-time%5C+live%5C+and%5C+produce.%5C+However%2C%5C+without%5C+written%5C+script%5C+of%5C+their%5C+own%5C+and%5C+with%5C+the%5C+influence%5C+of%5C+alien%5C+cultures%2C%5C+their%5C+indigenous%5C+knowledge%5C+is%5C+facing%5C+the%5C+risk%5C+of%5C+being%5C+assimilated%5C+or%5C+extinct.%5C+We%5C+carried%5C+out%5C+an%5C+ethnoparmacological%5C+investigation%5C+of%5C+medicinal%5C+plants%5C+in%5C+De%27%27ang%5C+communities.%5C+It%5C+has%5C+been%5C+found%5C+that%5C+92%5C+species%5C+were%5C+highly%5C+valuable%5C+for%5C+medicinal%5C+uses%5C+including%5C+heat%5C-clearing%5C+and%5C+detoxication%2C%5C+anti%5C-inflammatory%5C+pain%2C%5C+bone%5C+fracture%5C+and%5C+injury%2C%5C+relieving%5C+rigidity%5C+of%5C+muscles%5C+and%5C+promoting%5C+blood%5C+circulation%2C%5C+snake%5C+bite%5C+and%5C+so%5C+on.%5C+Of%5C+the%5C+92%5C+plants%5C+recorded%2C%5C+23%5C+species%5C+were%5C+newly%5C+recorded%5C+for%5C+the%5C+De%27%27ang%5C+people%2C%5C+and%5C+new%5C+medicinal%5C+properties%5C+of%5C+one%5C+plant%5C+species%5C+%5C%28Tacca%5C+chantrieri%5C%29%5C+was%5C+the%5C+first%5C+record%5C+too.%5C+In%5C+order%5C+to%5C+know%5C+well%5C+of%5C+the%5C+medicinal%5C+properties%5C+of%5C+traditional%5C+medicinal%5C+plants%5C+used%5C+by%5C+the%5C+De%27%27ang%5C+people%2C%5C+we%5C+selected%5C+three%5C+plants%5C+%5C%28Piper%5C+boehmeriaefolium%2C%5C+Remusatia%5C+vivipara%5C+and%5C+Pilea%5C+cavaleriei%5C+subsp.%5C+crenata%5C%29%5C+for%5C+the%5C+chemical%5C+constituents%5C+research.%5C+Forty%5C-six%5C+compounds%5C+including%5C+13%5C+new%5C+ones%5C+were%5C+isolated%5C+from%5C+the%5C+above%5C+mentioned%5C+folk%5C+medicinal%5C+plants.%5C+The%5C+types%5C+of%5C+these%5C+compounds%5C+involved%5C+amide%5C+alkaloids%2C%5C+lignans%2C%5C+phenylpropanoids%2C%5C+terpenoids%2C%5C+flavanoids%5C+and%5C+so%5C+on.%5C+In%5C+addition%2C%5C+some%5C+of%5C+the%5C+isolates%5C+were%5C+tested%5C+for%5C+their%5C+bioactivities.%5C+Thirty%5C+compounds%2C%5C+including%5C+8%5C+new%5C+ones%2C%5C+were%5C+isolated%5C+from%5C+the%5C+whole%5C+plant%5C+of%5C+Piper%5C+boehmeriaefolium%5C+%5C%28Piperaceae%5C%29.%5C+The%5C+compounds%5C+from%5C+the%5C+plant%5C+mainly%5C+belonged%5C+to%5C+the%5C+alkaloids%5C+of%5C+amides.%5C+A%5C+new%5C+phenylpropanoid%5C+glucoside%2C%5C+caffeyl%5C+alcohol%5C-3%5C-O%5C-b%5C-d%5C-glucopyranoside%2C%5C+together%5C+with%5C+nine%5C+known%5C+compounds%2C%5C+was%5C+isolated%5C+from%5C+the%5C+dry%5C+corms%5C+of%5C+Remusatia%5C+vivipara%5C+%5C%28Araceae%5C%29.%5C+The%5C+structure%5C+of%5C+the%5C+new%5C+compound%5C+was%5C+determined%5C+by%5C+the%5C+spectroscopic%5C+method%5C+and%5C+acidic%5C+hydrolysis.%5C+The%5C+compounds%5C+from%5C+the%5C+plant%5C+mainly%5C+belonged%5C+to%5C+phenlpropanoids%2C%5C+neolignans%2C%5C+steroid%5C+saponin%2C%5C+triterpenoid%5C+saponin%5C+and%5C+amides.%5C+All%5C+compounds%5C+were%5C+isolated%5C+from%5C+the%5C+genus%5C+Remusatia%5C+for%5C+the%5C+first%5C+time.%5C+Three%5C+new%5C+humulane%5C-type%5C+sesquiterpenes%2C%5C+and%5C+a%5C+new%5C+copaborneol%5C+derivative%2C%5C+%2C%5C+together%5C+with%5C+two%5C+known%5C+compounds%5C+have%5C+been%5C+isolated%5C+from%5C+the%5C+whole%5C+plant%5C+of%5C+Pilea%5C+cavaleriei%5C+subsp.%5C+crenata%5C+%5C%28Urticaceae%5C%29.%5C+All%5C+compounds%5C+were%5C+isolated%5C+from%5C+the%5C+genus%5C+Pilea%5C+for%5C+the%5C+first%5C+time."},{"jsname":"Trigonobalanus doichangensis is an endangered plant. In this paper, the megasporogenesis and development of female gametophyte, seed morphological traits and seed germination, seed conservation, micropropagation and acclimatization of this species were studied. Combined with the published results of cytology, molecular genetics and other researches,the mechanisms of extinction, basic biology and technology of germplasm conservation and acclimatization of T. doichangensis were discussed. The main results are summarized as follows:1. Megasporogenesis and development of female gametophyte，Stamens exist under the stigma of T. doichangensis, and the pollen is aborted on the later development stage of pistil, therefore, the pistillate flower in function is hermaphrodite flower in morphology. The ovule is anatropous, bitegmic and crassinucellate. The primary archesporium is hypodermal and single-celled and the sporogenous cell of the nucellus functions directly as a megaspore mother cell which goes meiosis to form a linear tetrad. The chalazal megaspore of the tetrad is functional. The development of embryo sac conforms to the polygonum type. There are six ovules in the ovary of T. doichangensis, and only one develops into a seed in normal fruits. In the process of megasporogenesis and development of female gametophyte, there are several links of abortion, and 93.3% of mature embryo sacs is aborted.2. Morphological characters and germination of seeds，Most of the variation occurred among individual trees within populations in seed morphological traits (length, width and 1000-seed weight) and germination-related indices (germination percentage, germination index and vigor index). In addition, the variation in percentage of well-developed seeds among populations and among individual trees within populations is equal, each accounting for 48%. Each of seed morphological traits has significantly positive correlation with each other (p < 0.01), but they have no significant correlation with percentage of well-developed seeds and germination-related indices. In the same batch of seeds of T. doichangensis, there are light-colored and dark-colored seed coats, and development of light-colored seeds is significantly poorer than that of dark-colored seeds.The sensitivity of seeds to high temperature varys in different stages of seed imbibition. In each stage, heat acclimatization don’t increase germination percentage, germination index and fresh weight of seedlings. If the distilled water is substituted by solution of SA during seed imbibition, seed germination and germination index after heat shock are not significantly different from control, but they are significantly higher than that of other treatments. Moreover, when the seeds are treatmented with SA, the fresh weight of seedlings is significantly higher than that of control and other treatments.3. Seed conservation，Seeds of T. doichangensis belong to orthodox seeds which can tolerate certain level of dehydration. The condition of low temperature and low water content of seeds is conducive to seed conservation.Germination of fresh seeds shows significant variation among populations, howerer, germination of the seeds after storage for one year in room temperature shows no significant variation among populations.High temperature and high relative humidity damages the seeds more severely than high temperature does. In addition, low water content of seeds enable the seeds to be more tolerant to high temperature.The electrical conductivity, dehydrogenase activity and germination percentage have no significant correlation with each other.4. Micropropagation and in vitro conservation，Cotyledonary nodes are a kind of efficient explants. Low salt media are conducive to shoot propagation and root induction.The maximum multiplication rate (20-25 shoots/explant within 4 months) is achieved on quarter-strength Murashige and Skoog (1/4 MS) medium supplemented with 1 mg·L-1 6-benzyladenine (6-BA) and 0.05 mg·L-1 α-naphthaleneacetic acid (NAA).Rooting is promoted by auxins, however, IBA alone or low concentrations of NAA are preferable due to small amount of callus induced. The research has established an efficient protocol for micropropagation of T. doichangensis, and it provides technology support for in vitro conservation of special germplasm of the species.5. Acclimatization，Quercus variabilis, Cyclobalanopsis glaucoides and T. doichangensis belong to the family of Fagaceae, and the natural distribution ranges of the 3 species are decreasing in turn. The research suggests that the ranges of temperature tolerance of the 3 species are decreasing corresponding to their distribution ranges.The high and low semi-lethal temperature of one-year old T. doichangensis is 49.5℃ and -5℃ respectively. It suggests that T. doichangensis has a wide range of basic temperature tolerance. Short-term heat and cold acclimatization cannot expand the range of temperature tolerance. It can be inferred that T. doichangensis may lack induced tolerance to temperature. Under proper conditions, ABA can increase the cold tolerance, and SA can increase the heat tolerance of leaf discs of T. 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