×
验证码:
换一张
忘记密码？
记住我
×
登录
中文版
|
English
中国科学院昆明植物研究所知识管理系统
Knowledge Management System of Kunming Institute of Botany,CAS
登录
注册
ALL
ORCID
题名
作者
学科领域
关键词
资助项目
文献类型
出处
收录类别
出版者
发表日期
存缴日期
学科门类
学习讨论厅
图片搜索
粘贴图片网址
首页
研究单元&专题
作者
文献类型
学科分类
知识图谱
新闻&公告
研究单元&专题
中国科学院东亚植... [142]
昆明植物所硕博研究... [88]
资源植物与生物技术... [72]
共享文献 [41]
中国西南野生生物种... [17]
植物化学与西部植物... [10]
更多...
作者
许建初 [49]
孙航 [37]
李德铢 [33]
孙卫邦 [25]
龚洵 [17]
高连明 [16]
更多...
文献类型
期刊论文 [381]
学位论文 [88]
专著 [25]
会议录 [2]
会议论文 [1]
其他 [1]
更多...
发表日期
2021 [40]
2020 [71]
2019 [67]
2018 [40]
2017 [37]
2016 [32]
更多...
语种
英语 [243]
中文 [68]
出处
FUNGAL DI... [38]
PHYTOTAXA [19]
JOURNAL O... [13]
MYCOSPHER... [12]
PLANT DIV... [12]
PLOS ONE [12]
更多...
资助项目
0.05). For some populations, germination capacity in 12-h photoperiod was significantly higher than that in completed darkness（W-FD: P < 0.01, W-JD: P < 0.05）.Genetic variation within and among six populations was assessed using AFLP markers. Genetic diversity was higher at species level (PPL = 69.19%, HE = 0.221) than at population level (PPL = 26.22%, HE = 0.095, Is =0.140), and populations in southeast Yunnan were strongly differentiated from those in southwest Yunnan (Nei’s GST = 0.575; FST = 0.655). UPGMA analysis demonstrated a clear genetic division between the two populations from DeHong (SW Yunnan; D-JD and D-HG) and the four from WenShan (SE Yunnan; W-FD, W-LH, W-ML, and W-MG). Within-population genetic variation was significantly correlated with population isolation (r(PPL) = -0.94, P = 0.006; r(HE) = -0.85, P = 0.032; r(Is) = -0.87, P = 0.025), but not with population size (r(PPL) = 0.63, P = 0.178; r(HE) = 0.54, P = 0.268; r(Is) = 0.56, P = 0.249).","jscount":"1","jsurl":"/simple-search?field1=all&field=dc.type_filter&advanced=false&query1=China%2BBiodiversity%2BRed%2BList-Higher%2BPlants&&fq=dc.project.title_filter%3ACraigia%5C+yunnanensis%5C+W.%5C+W.%5C+Smith%5C+%5C%26%5C+W.%5C+E.%5C+Evans%5C+%5C%28Tiliaceae%5C%29%5C+is%5C+an%5C+endangered%5C+deciduous%5C+tree%5C+species%5C+which%5C+has%5C+high%5C+scientific%5C+and%5C+economic%5C+value.%5C+C.%5C+yunnanensis%5C+is%5C+seriously%5C+threatened%5C+and%5C+has%5C+been%5C+pushed%5C+to%5C+the%5C+verge%5C+of%5C+extinction%5C+due%5C+to%5C+vegetation%5C+destruction%5C+in%5C+China%5C+and%5C+consequent%5C+contraction%5C+of%5C+its%5C+distribution.%5C+Hence%2C%5C+it%5C+was%5C+listed%5C+as%5C+a%5C+nationally%5C+rare%5C+and%5C+endangered%5C+plant%5C+in%5C+1999%5C+and%5C+has%5C+also%5C+been%5C+proposed%5C+as%5C+a%5C+second%5C-ranked%5C+plant%5C+for%5C+national%5C+protection%5C+in%5C+China%5C+and%5C+included%5C+in%5C+IUCN%5C+red%5C+list.%5C+As%5C+a%5C+scientifically%5C+important%5C+and%5C+valued%5C+tree%5C+species%5C+with%5C+endangered%5C+status%2C%5C+the%5C+wild%5C+populations%5C+of%5C+C.%5C+yunnanensis%5C+therefore%5C+represent%5C+is%5C+a%5C+genetic%5C+resource%5C+that%5C+must%5C+be%5C+conserved.%5C+To%5C+provide%5C+basic%5C+information%5C+for%5C+its%5C+conservation%2C%5C+the%5C+population%5C+dynamics%5C+and%5C+population%5C+size%5C+structures%2C%5C+pollination%5C+biology%5C+and%5C+breeding%5C+system%2C%5C+eleven%5C+fitness%5C-related%5C+characters%5C+and%5C+the%5C+genetic%5C+variability%5C+based%5C+on%5C+AFLP%5C+were%5C+comprehensively%5C+studied.%5C+The%5C+main%5C+results%5C+are%5C+summarized%5C+as%5C+follows%5C%3A%5C+A%5C+total%5C+of%5C+six%5C+wild%5C+populations%5C+of%5C+C.%5C+yunnanensis%5C+were%5C+found%5C+in%5C+two%5C+disjunct%5C+regions%5C+of%5C+Yunnan%2C%5C+i.e.%5C+WenShan%5C+%5C%28SE%5C+Yunnan%5C%29%5C+and%5C+DeHong%5C+%5C%28SW%5C+Yunnan%5C%29%2C%5C+from%5C+2005%5C+to%5C+2007.%5C+Additionally%2C%5C+in%5C+all%5C+but%5C+one%5C+of%5C+the%5C+populations%5C+we%5C+detected%2C%5C+mature%5C+trees%5C+were%5C+felled%5C+between%5C+2005%5C+and%5C+2007%2C%5C+so%5C+destruction%5C+of%5C+most%5C+of%5C+these%5C+populations%5C+is%5C+ongoing.%5C+Across%5C+the%5C+six%5C+populations%5C+of%5C+extant%5C+C.%5C+yunnanensis%5C+found%5C+during%5C+our%5C+study%2C%5C+the%5C+total%5C+number%5C+of%5C+mature%5C+%5C%28reproductive%5C%29%5C+individuals%5C+detected%5C+was%5C+584%5C+in%5C+2007%EF%BC%8Cplus%5C+larger%5C+numbers%5C+of%5C+seedling%5C+and%5C+resprouts%5C+from%5C+cut%5C+trunks.%5C+The%5C+result%5C+of%5C+surveying%5C+Population%5C+structure%5C+showed%5C+that%5C+there%5C+are%5C+two%5C+regeneration%5C+types%5C+which%5C+are%5C+seedlings%5C+and%5C+sprouts.%5C+Seedlings%5C+occurred%5C+abundantly%5C+in%5C+gaps%5C+or%5C+open%5C+areas%5C+and%5C+the%5C+size%5C+class%5C+frequency%5C+distributions%5C+were%5C+often%5C+discontinuous%2C%5C+and%5C+the%5C+same%5C+general%5C+pattern%5C+occurred%5C+in%5C+all%5C+the%5C+investigated%5C+populations%5C+for%5C+juveniles%5C+and%5C+adults.%5C+The%5C+numbers%5C+of%5C+seed%5C-origin%5C+individuals%5C+did%5C+however%5C+decline%5C+sharply%5C+with%5C+increasing%5C+size%2C%5C+indicating%5C+a%5C+high%5C+mortality%5C+rate%5C+going%5C+from%5C+seedling%5C+to%5C+sapling%5C+stage%5C+may%5C+be%5C+a%5C+problem%5C+for%5C+this%5C+species.%5C+Additionally%2C%5C+the%5C+cash%5C+crop%5C+cultivation%5C+and%5C+logging%5C+seriously%5C+threaten%5C+the%5C+survival%5C+of%5C+the%5C+species.%5C+We%5C+conducted%5C+field%5C+observations%5C+and%5C+artificial%5C+pollination%5C+experiments%5C+on%5C+the%5C+floral%5C+biology%2C%5C+pollination%5C+process%5C+and%5C+breeding%5C+system%5C+of%5C+Craigia%5C+yunnanensis%5C+in%5C+Fadou%2C%5C+Xichou%5C+county%5C+of%5C+Yunnan%5C+province.%5C+The%5C+lifespan%5C+of%5C+a%5C+single%5C+hermaphrodite%5C+flower%5C+is%5C+approximately%5C+3%5C-4%5C+days.%5C+A%5C+cyme%5C+has%5C+2%5C-9%5C+flowered.%5C+The%5C+flowering%5C+period%5C+of%5C+an%5C+inflorescence%5C+is%5C+usually%5C+5%5C-14%5C+days.%5C+The%5C+flowers%5C+of%5C+C.%5C+yunnanensis%5C+were%5C+protandrous.%5C+The%5C+stamens%5C+were%5C+within%5C+petal%5C-like%5C+staminodes%5C+in%5C+the%5C+opening%5C+flowers%5C+until%5C+the%5C+flower%5C+withered.%5C+Without%5C+touchment%2C%5C+the%5C+bractlike%5C+staminodes%5C+can%E2%80%99t%5C+open.%5C+Self%5C-pollination%5C+was%5C+partially%5C+avoided%5C+by%5C+temporal%5C+and%5C+spatial%5C+isolation%5C+of%5C+male%5C+and%5C+female%5C+organs%5C+within%5C+the%5C+same%5C+flower.%5C+However%2C%5C+autogamous%5C+and%5C+geitonogamous%5C+pollination%5C+is%5C+unavoidable%5C+because%5C+of%5C+the%5C+large%5C+number%5C+of%5C+flowers%5C+on%5C+a%5C+single%5C+tree%5C+and%5C+the%5C+action%5C+of%5C+pollinators.%5C+The%5C+values%5C+of%5C+both%5C+OCI%5C+%5C%28%E2%89%A54%5C%29%5C+and%5C+P%5C%2FO%5C+%5C%281381%5C%29%5C+and%5C+the%5C+results%5C+of%5C+bagging%5C+tests%5C+indicated%5C+there%5C+was%5C+no%5C+apomixes%5C+in%5C+C.%5C+yunnanensis%5C+and%5C+the%5C+breeding%5C+system%5C+of%5C+the%5C+species%5C+was%5C+outcrossing%5C+with%5C+partial%5C+self%5C-compatibility%5C+and%5C+the%5C+pollinators%5C+were%5C+required%5C+during%5C+the%5C+pollination%5C+process.%5C+The%5C+most%5C+frequent%5C+effective%5C+floral%5C+visitor%5C+was%5C+only%5C+beautiful%5C+fly%5C+%5C%28Chrysomyia%5C+megacephala%5C%29.%5C+Fruit%5C+set%5C+and%5C+seed%5C+set%5C+in%5C+natural%5C+condition%5C+were%5C+56.67%C2%B13.85%EF%BC%85%5C+and%5C+6.26%C2%B10.75%EF%BC%85%2C%5C+respectively.%5C+Therefore%2C%5C+lack%5C+of%5C+pollinators%2C%5C+low%5C+pollination%5C+efficiency%2C%5C+unavoidable%5C+geitonogamous%5C+pollination%5C+and%5C+partial%5C+self%5C-compatibility%5C+and%5C+inbreeding%5C+in%5C+small%5C+populations%5C+may%5C+account%5C+for%5C+the%5C+low%5C+fruit%5C+set%2C%5C+especially%5C+seed%5C+set.Variations%5C+in%5C+seed%5C+traits%2C%5C+seed%5C+germination%2C%5C+and%5C+seedling%5C+growth%5C+characters%5C+among%5C+six%5C+Craigia%5C+yunnanensis%5C+populations%5C+were%5C+evaluated.%5C+All%5C+seed%5C+and%5C+seedling%5C+traits%5C+exhibited%5C+significant%5C+differences%5C+among%5C+populations%5C+%5C%28P%5C+%3C%5C+0.05%5C%29.%5C+The%5C+fitness%5C+of%5C+seed%5C+as%5C+assessed%5C+by%5C+seed%5C+size%2C%5C+seed%5C+germination%5C+and%5C+seedling%5C+trait%5C+was%5C+independent%5C+of%5C+population%5C+size%2C%5C+except%5C+for%5C+the%5C+number%5C+of%5C+seeds%5C+per%5C+capsule%5C+%5C%28r%5C+%3D%5C+0.93%EF%BC%8CP%5C+%3C%5C+0.01%5C%29.%5C+Correlations%5C+between%5C+geo%5C-climatic%5C+variables%5C+of%5C+seed%5C+origin%5C+and%5C+seed%5C+and%5C+seedling%5C+related%5C+characters%5C+were%5C+insignificant%5C+%5C%28P%5C+%3E%5C+0.05%5C%29.%5C+For%5C+some%5C+populations%2C%5C+germination%5C+capacity%5C+in%5C+12%5C-h%5C+photoperiod%5C+was%5C+significantly%5C+higher%5C+than%5C+that%5C+in%5C+completed%5C+darkness%EF%BC%88W%5C-FD%5C%3A%5C+P%5C+%3C%5C+0.01%2C%5C+W%5C-JD%5C%3A%5C+P%5C+%3C%5C+0.05%EF%BC%89.Genetic%5C+variation%5C+within%5C+and%5C+among%5C+six%5C+populations%5C+was%5C+assessed%5C+using%5C+AFLP%5C+markers.%5C+Genetic%5C+diversity%5C+was%5C+higher%5C+at%5C+species%5C+level%5C+%5C%28PPL%5C+%3D%5C+69.19%25%2C%5C+HE%5C+%3D%5C+0.221%5C%29%5C+than%5C+at%5C+population%5C+level%5C+%5C%28PPL%5C+%3D%5C+26.22%25%2C%5C+HE%5C+%3D%5C+0.095%2C%5C+Is%5C+%3D0.140%5C%29%2C%5C+and%5C+populations%5C+in%5C+southeast%5C+Yunnan%5C+were%5C+strongly%5C+differentiated%5C+from%5C+those%5C+in%5C+southwest%5C+Yunnan%5C+%5C%28Nei%E2%80%99s%5C+GST%5C+%3D%5C+0.575%5C%3B%5C+FST%5C+%3D%5C+0.655%5C%29.%5C+UPGMA%5C+analysis%5C+demonstrated%5C+a%5C+clear%5C+genetic%5C+division%5C+between%5C+the%5C+two%5C+populations%5C+from%5C+DeHong%5C+%5C%28SW%5C+Yunnan%5C%3B%5C+D%5C-JD%5C+and%5C+D%5C-HG%5C%29%5C+and%5C+the%5C+four%5C+from%5C+WenShan%5C+%5C%28SE%5C+Yunnan%5C%3B%5C+W%5C-FD%2C%5C+W%5C-LH%2C%5C+W%5C-ML%2C%5C+and%5C+W%5C-MG%5C%29.%5C+Within%5C-population%5C+genetic%5C+variation%5C+was%5C+significantly%5C+correlated%5C+with%5C+population%5C+isolation%5C+%5C%28r%5C%28PPL%5C%29%5C+%3D%5C+%5C-0.94%2C%5C+P%5C+%3D%5C+0.006%5C%3B%5C+r%5C%28HE%5C%29%5C+%3D%5C+%5C-0.85%2C%5C+P%5C+%3D%5C+0.032%5C%3B%5C+r%5C%28Is%5C%29%5C+%3D%5C+%5C-0.87%2C%5C+P%5C+%3D%5C+0.025%5C%29%2C%5C+but%5C+not%5C+with%5C+population%5C+size%5C+%5C%28r%5C%28PPL%5C%29%5C+%3D%5C+0.63%2C%5C+P%5C+%3D%5C+0.178%5C%3B%5C+r%5C%28HE%5C%29%5C+%3D%5C+0.54%2C%5C+P%5C+%3D%5C+0.268%5C%3B%5C+r%5C%28Is%5C%29%5C+%3D%5C+0.56%2C%5C+P%5C+%3D%5C+0.249%5C%29."},{"jsname":"Cyatheaceae species, usually called tree ferns, are considered as relicts of a time when dinosaurs were common. In recent several decades, the number of Cyatheaceae plants decreases dramatically. In order to find the reasons and provide directions for protecting these endangered plants, the biological characteristics of Cyatheaceae were surveyed. Using AFLP and cpDNA sequence variations, the genetic diversity and phylogeography of Sphaeropteris brunoniana were also analyzed. Based on these findings, implications for conservation strategies were discussed for this relict tree fern. Main results of the dissertation were summarized as follows, (1) Cyatheaceae plants have extensive distribution in Yunnan, China, and most of them distribute in southeast of Yunnan. In southeast, they usually inhabit margins of evergreen broad-leaved forests or secondary coniferous forests; however, the population update is very different and the age structure is unscientific. The spore of Cyatheaceae is trilete, radially symmetrical, and perinous. The spores of Alsophila species feature a ridged perine and a granular, verrucate or smooth exine. The spores of S. brunoniana are characterized by an incipient granular outermost layer and a verrucate exine. The metaphase chromosome numbers of gametophytes in the three examined species, viz. A. podophylla, A. gigantea and A. austro-yunnanensis, are 69, indicating that they are diploid and do not display variety in chromosome number. The chemical constituents of S. brunoniana are main simple and familiar compounds, such as saccharides, fatty acids and alcohols, and stigmasterols. (2) An unexpectedly high level of nDNA genetic diversity and low cpDNA diversity were detected in S. brunoniana. (3) This study showed that the genetic differentiation among populations within regions was low and between regions was significant. (4) There were several refugia of S. brunoniana in Yunnan during glacial periods. The Hainan populations were likely new colonizations and originated from Southeast Asia. (5) To retain existing genetic diversity, whether in situ or ex situ conservation or collection of germplasm is used, the populations of the two regions should be considered equally. Furthermore, ex situ conservation of this species should be preferably conducted on large populations.","jscount":"1","jsurl":"/simple-search?field1=all&field=dc.type_filter&advanced=false&query1=China%2BBiodiversity%2BRed%2BList-Higher%2BPlants&&fq=dc.project.title_filter%3ACyatheaceae%5C+species%2C%5C+usually%5C+called%5C+tree%5C+ferns%2C%5C+are%5C+considered%5C+as%5C+relicts%5C+of%5C+a%5C+time%5C+when%5C+dinosaurs%5C+were%5C+common.%5C+In%5C+recent%5C+several%5C+decades%2C%5C+the%5C+number%5C+of%5C+Cyatheaceae%5C+plants%5C+decreases%5C+dramatically.%5C+In%5C+order%5C+to%5C+find%5C+the%5C+reasons%5C+and%5C+provide%5C+directions%5C+for%5C+protecting%5C+these%5C+endangered%5C+plants%2C%5C+the%5C+biological%5C+characteristics%5C+of%5C+Cyatheaceae%5C+were%5C+surveyed.%5C+Using%5C+AFLP%5C+and%5C+cpDNA%5C+sequence%5C+variations%2C%5C+the%5C+genetic%5C+diversity%5C+and%5C+phylogeography%5C+of%5C+Sphaeropteris%5C+brunoniana%5C+were%5C+also%5C+analyzed.%5C+Based%5C+on%5C+these%5C+findings%2C%5C+implications%5C+for%5C+conservation%5C+strategies%5C+were%5C+discussed%5C+for%5C+this%5C+relict%5C+tree%5C+fern.%5C+Main%5C+results%5C+of%5C+the%5C+dissertation%5C+were%5C+summarized%5C+as%5C+follows%2C%5C+%5C%281%5C%29%5C+Cyatheaceae%5C+plants%5C+have%5C+extensive%5C+distribution%5C+in%5C+Yunnan%2C%5C+China%2C%5C+and%5C+most%5C+of%5C+them%5C+distribute%5C+in%5C+southeast%5C+of%5C+Yunnan.%5C+In%5C+southeast%2C%5C+they%5C+usually%5C+inhabit%5C+margins%5C+of%5C+evergreen%5C+broad%5C-leaved%5C+forests%5C+or%5C+secondary%5C+coniferous%5C+forests%5C%3B%5C+however%2C%5C+the%5C+population%5C+update%5C+is%5C+very%5C+different%5C+and%5C+the%5C+age%5C+structure%5C+is%5C+unscientific.%5C+The%5C+spore%5C+of%5C+Cyatheaceae%5C+is%5C+trilete%2C%5C+radially%5C+symmetrical%2C%5C+and%5C+perinous.%5C+The%5C+spores%5C+of%5C+Alsophila%5C+species%5C+feature%5C+a%5C+ridged%5C+perine%5C+and%5C+a%5C+granular%2C%5C+verrucate%5C+or%5C+smooth%5C+exine.%5C+The%5C+spores%5C+of%5C+S.%5C+brunoniana%5C+are%5C+characterized%5C+by%5C+an%5C+incipient%5C+granular%5C+outermost%5C+layer%5C+and%5C+a%5C+verrucate%5C+exine.%5C+The%5C+metaphase%5C+chromosome%5C+numbers%5C+of%5C+gametophytes%5C+in%5C+the%5C+three%5C+examined%5C+species%2C%5C+viz.%5C+A.%5C+podophylla%2C%5C+A.%5C+gigantea%5C+and%5C+A.%5C+austro%5C-yunnanensis%2C%5C+are%5C+69%2C%5C+indicating%5C+that%5C+they%5C+are%5C+diploid%5C+and%5C+do%5C+not%5C+display%5C+variety%5C+in%5C+chromosome%5C+number.%5C+The%5C+chemical%5C+constituents%5C+of%5C+S.%5C+brunoniana%5C+are%5C+main%5C+simple%5C+and%5C+familiar%5C+compounds%2C%5C+such%5C+as%5C+saccharides%2C%5C+fatty%5C+acids%5C+and%5C+alcohols%2C%5C+and%5C+stigmasterols.%5C+%5C%282%5C%29%5C+An%5C+unexpectedly%5C+high%5C+level%5C+of%5C+nDNA%5C+genetic%5C+diversity%5C+and%5C+low%5C+cpDNA%5C+diversity%5C+were%5C+detected%5C+in%5C+S.%5C+brunoniana.%5C+%5C%283%5C%29%5C+This%5C+study%5C+showed%5C+that%5C+the%5C+genetic%5C+differentiation%5C+among%5C+populations%5C+within%5C+regions%5C+was%5C+low%5C+and%5C+between%5C+regions%5C+was%5C+significant.%5C+%5C%284%5C%29%5C+There%5C+were%5C+several%5C+refugia%5C+of%5C+S.%5C+brunoniana%5C+in%5C+Yunnan%5C+during%5C+glacial%5C+periods.%5C+The%5C+Hainan%5C+populations%5C+were%5C+likely%5C+new%5C+colonizations%5C+and%5C+originated%5C+from%5C+Southeast%5C+Asia.%5C+%5C%285%5C%29%5C+To%5C+retain%5C+existing%5C+genetic%5C+diversity%2C%5C+whether%5C+in%5C+situ%5C+or%5C+ex%5C+situ%5C+conservation%5C+or%5C+collection%5C+of%5C+germplasm%5C+is%5C+used%2C%5C+the%5C+populations%5C+of%5C+the%5C+two%5C+regions%5C+should%5C+be%5C+considered%5C+equally.%5C+Furthermore%2C%5C+ex%5C+situ%5C+conservation%5C+of%5C+this%5C+species%5C+should%5C+be%5C+preferably%5C+conducted%5C+on%5C+large%5C+populations."},{"jsname":"Cycas micholitzii complex is composed of 5 species: C. micholitzii Dyer, C. bifida (Dyer) K. D. Hill,C. longipetiolula D. Y. Wang, C. debaoensis Y. C. Zhong et C J. Chen, C. multipinnata C J. Chen et S. Y. Yang，and distributed from southwest China to central Vietnam and eastern Laos. Based on sequence data from two maternally inherited cpDNA and one biparentally nuclear DNA fragments, our study revealed the population genetic structure of C. micholitzii complex and explored the potential causes. The evolutionary and demographic histories were investigated. The genetic relationship among species in the complex was also clarified.The results were summarized as follows: 1. Phylogeographic analysis based on chloroplast sequences，We examined chloroplast sequence variation of the atpB-rbcLand psbA-trnHintergenic spacers in 27 populations of C. micholitzii complex, recovering 26 haplotypes. The average within-population diversity (HS = 0.140) was low while total diversity (HT = 0.911) was high. Population differentiation was also high(GST = 0.846, NST = 0.919), indicating significant phylogeographical structure (NST > GST,p < 0.001) and low levels of seed-based gene flow. C. debaoensis (Cycadaceae) is an endangered species restricted to the border of Guangxi and Yunnan province in southwest China. This species has been classified into two types: sand and karst, according to the soil matrix they grow on. We examined chloroplast sequence variation of the cpDNA sequences from 11 populations of this species. Significant population genetic differentiation was detected (GST= 0.684 and FST = 0.74160). There was marked genetic differentiation between populations in the sand and karst regions and no expansion was detected. Climate changes during glacial periods have had significant effects on the current distribution of cycads. The molecular phylogenetic data, together with the geographic distribution of the haplotypes, suggest that C. debaoensis experienced range contraction during glacial periods, and that the current populations are still confined to the original refugia in southwest China which have favorable habitats in glacial period. These results imply that small refugia were maintained in both sand and karst regions during the LGM (last glacial maximum). This species had no postglacial recolonization and only stayed in these refugia up to now. The low within-population diversity of C. debaoensis suggests that there were strong bottleneck events or founder effects within each separate region during the Quaternary climatic oscillations. Relatively high genetic and haplotype diversities were detected in the newly discovered populations, which located at intermediate locality of sand regions and had morphological variation; this is probably the consequence of the admixture of different haplotypes colonizing the area from separate sources. C. micholitzii occurs in the Annan Highlands in central Vietnam near the Laos border. C. bifida occurs in North Vietnam; its distribution extends across the border into adjacent localities in Guangxi and Yunnan in China. For the comparability between them,theywere considered as the same species C. micholitzii by many academicians. The cpDNA sequences from 11 populations showed that these very controversial species, C. micholitzii and C. bifida, is paraphyletic and should belong to the same species C. micholitzii. AMOVA analysis showed that the component of among-population within region/species (76.46%) was unexpectedly larger than the among-species/region component (14.97%), which also indicates that there is no justification for recognizing two species as C. micholitzii and C. bifida. This hypothesis was also supported by the geological data, especially the neotectonic history of the indo-china block, which started to move south since Oligocene and cause the geographic isolation of these two groups. Therefore, the most likely explanation to the phenotypic similarities between these two groups may be the retention of ancestral polymorphisms in the paraphyletic group due to incomplete lineage sorting. Furthermore, the similarities may also be ascribed to pollen-mediated gene flow among geographically proximate populations and/or phenotypic convergence under similar selection schemes in the same region. C.micholitzi had the higest genetic diversity (HT = 0.980,) and genetic differentiation (GST = 0.830, NST = 0.915) among the C. micholitzii complex. The high genetic diversity might be attributed to its long evolutionary history, highly diverse habitats. The ineffective mode of seed dispersal and dramatic neotectonic movement in the distribution range of this species could result in the high genetic differentiation. 2. Phylogeographic analysis based on nuclear ribosomal sequences, We sequenced the nrDNA ITS in all 27 populations sampled, 7 haplotypes were identified, among which C. micholitzii had 6, while C. multipinnata, C. longipetiolula and C. debaoensis shared the remaining one. Compared to chloroplast genes, nuclear genes had higher correlation between genetic and geographical distance, but lower interspecies differentiation (54.42% vs 25.24%). Phylogeographical structure of C. micholitzii and C.bifida based on ITS Variation was consistent with the morphology differentiation. This similar in nuclear gene should be ascribed to pollen-mediated gene flow among geographically proximate populations.Long-distance gene flow over the two groups was clearly interrupted, which brought on the nrDNA genetic differenciation between the geographically isolated groups, to a certain extent affected the morphological variation. 3. Interspecies relationships among Cycas micholitzii complex, We analysed chloroplast sequence variation of the atpB-rbcL and psbA-trnH intergenic spacers in 27 populations sampled of C. micholitzii complex, AMOVA analysis showed that the component of among-species/region component (59.21%). However, phylogenic analysis showed that the haplotypes of C. micholitzii complex couldn`t grouped into four clusters closely corresponding to the narrowly defined C. micholitzi, C. multipinnata, C. debaoensis and C. longipetiolula. We concluded that the conflict may result from several factors: firstly incomplete lineage sorting of C. micholitzii; secondly hybridization/introgression of sympatrically cycads, which would be supported by evidence base on nrDNA ITS sequences; thirdly intramolecular recombination in cpDNA of cycads; eventually the neotectonic movement in the distribution range of this species.","jscount":"1","jsurl":"/simple-search?field1=all&field=dc.type_filter&advanced=false&query1=China%2BBiodiversity%2BRed%2BList-Higher%2BPlants&&fq=dc.project.title_filter%3ACycas%5C+micholitzii%5C+complex%5C+is%5C+composed%5C+of%5C+5%5C+species%5C%3A%5C+C.%5C+micholitzii%5C+Dyer%2C%5C+C.%5C+bifida%5C+%5C%28Dyer%5C%29%5C+K.%5C+D.%5C+Hill%2CC.%5C+longipetiolula%5C+D.%5C+Y.%5C+Wang%2C%5C+C.%5C+debaoensis%5C+Y.%5C+C.%5C+Zhong%5C+et%5C+C%5C+J.%5C+Chen%2C%5C+C.%5C+multipinnata%5C+C%5C+J.%5C+Chen%5C+et%5C+S.%5C+Y.%5C+Yang%EF%BC%8Cand%5C+distributed%5C+from%5C+southwest%5C+China%5C+to%5C+central%5C+Vietnam%5C+and%5C+eastern%5C+Laos.%5C+Based%5C+on%5C+sequence%5C+data%5C+from%5C+two%5C+maternally%5C+inherited%5C+cpDNA%5C+and%5C+one%5C+biparentally%5C+nuclear%5C+DNA%5C+fragments%2C%5C+our%5C+study%5C+revealed%5C+the%5C+population%5C+genetic%5C+structure%5C+of%5C+C.%5C+micholitzii%5C+complex%5C+and%5C+explored%5C+the%5C+potential%5C+causes.%5C+The%5C+evolutionary%5C+and%5C+demographic%5C+histories%5C+were%5C+investigated.%5C+The%5C+genetic%5C+relationship%5C+among%5C+species%5C+in%5C+the%5C+complex%5C+was%5C+also%5C+clarified.The%5C+results%5C+were%5C+summarized%5C+as%5C+follows%5C%3A%5C+1.%5C+Phylogeographic%5C+analysis%5C+based%5C+on%5C+chloroplast%5C+sequences%EF%BC%8CWe%5C+examined%5C+chloroplast%5C+sequence%5C+variation%5C+of%5C+the%5C+atpB%5C-rbcLand%5C+psbA%5C-trnHintergenic%5C+spacers%5C+in%5C+27%5C+populations%5C+of%5C+C.%5C+micholitzii%5C+complex%2C%5C+recovering%5C+26%5C+haplotypes.%5C+The%5C+average%5C+within%5C-population%5C+diversity%5C+%5C%28HS%5C+%3D%5C+0.140%5C%29%5C+was%5C+low%5C+while%5C+total%5C+diversity%5C+%5C%28HT%5C+%3D%5C+0.911%5C%29%5C+was%5C+high.%5C+Population%5C+differentiation%5C+was%5C+also%5C+high%5C%28GST%5C+%3D%5C+0.846%2C%5C+NST%5C+%3D%5C+0.919%5C%29%2C%5C+indicating%5C+significant%5C+phylogeographical%5C+structure%5C+%5C%28NST%5C+%3E%5C+GST%2Cp%5C+%3C%5C+0.001%5C%29%5C+and%5C+low%5C+levels%5C+of%5C+seed%5C-based%5C+gene%5C+flow.%5C+C.%5C+debaoensis%5C+%5C%28Cycadaceae%5C%29%5C+is%5C+an%5C+endangered%5C+species%5C+restricted%5C+to%5C+the%5C+border%5C+of%5C+Guangxi%5C+and%5C+Yunnan%5C+province%5C+in%5C+southwest%5C+China.%5C+This%5C+species%5C+has%5C+been%5C+classified%5C+into%5C+two%5C+types%5C%3A%5C+sand%5C+and%5C+karst%2C%5C+according%5C+to%5C+the%5C+soil%5C+matrix%5C+they%5C+grow%5C+on.%5C+We%5C+examined%5C+chloroplast%5C+sequence%5C+variation%5C+of%5C+the%5C+cpDNA%5C+sequences%5C+from%5C+11%5C+populations%5C+of%5C+this%5C+species.%5C+Significant%5C+population%5C+genetic%5C+differentiation%5C+was%5C+detected%5C+%5C%28GST%3D%5C+0.684%5C+and%5C+FST%5C+%3D%5C+0.74160%5C%29.%5C+There%5C+was%5C+marked%5C+genetic%5C+differentiation%5C+between%5C+populations%5C+in%5C+the%5C+sand%5C+and%5C+karst%5C+regions%5C+and%5C+no%5C+expansion%5C+was%5C+detected.%5C+Climate%5C+changes%5C+during%5C+glacial%5C+periods%5C+have%5C+had%5C+significant%5C+effects%5C+on%5C+the%5C+current%5C+distribution%5C+of%5C+cycads.%5C+The%5C+molecular%5C+phylogenetic%5C+data%2C%5C+together%5C+with%5C+the%5C+geographic%5C+distribution%5C+of%5C+the%5C+haplotypes%2C%5C+suggest%5C+that%5C+C.%5C+debaoensis%5C+experienced%5C+range%5C+contraction%5C+during%5C+glacial%5C+periods%2C%5C+and%5C+that%5C+the%5C+current%5C+populations%5C+are%5C+still%5C+confined%5C+to%5C+the%5C+original%5C+refugia%5C+in%5C+southwest%5C+China%5C+which%5C+have%5C+favorable%5C+habitats%5C+in%5C+glacial%5C+period.%5C+These%5C+results%5C+imply%5C+that%5C+small%5C+refugia%5C+were%5C+maintained%5C+in%5C+both%5C+sand%5C+and%5C+karst%5C+regions%5C+during%5C+the%5C+LGM%5C+%5C%28last%5C+glacial%5C+maximum%5C%29.%5C+This%5C+species%5C+had%5C+no%5C+postglacial%5C+recolonization%5C+and%5C+only%5C+stayed%5C+in%5C+these%5C+refugia%5C+up%5C+to%5C+now.%5C+The%5C+low%5C+within%5C-population%5C+diversity%5C+of%5C+C.%5C+debaoensis%5C+suggests%5C+that%5C+there%5C+were%5C+strong%5C+bottleneck%5C+events%5C+or%5C+founder%5C+effects%5C+within%5C+each%5C+separate%5C+region%5C+during%5C+the%5C+Quaternary%5C+climatic%5C+oscillations.%5C+Relatively%5C+high%5C+genetic%5C+and%5C+haplotype%5C+diversities%5C+were%5C+detected%5C+in%5C+the%5C+newly%5C+discovered%5C+populations%2C%5C+which%5C+located%5C+at%5C+intermediate%5C+locality%5C+of%5C+sand%5C+regions%5C+and%5C+had%5C+morphological%5C+variation%5C%3B%5C+this%5C+is%5C+probably%5C+the%5C+consequence%5C+of%5C+the%5C+admixture%5C+of%5C+different%5C+haplotypes%5C+colonizing%5C+the%5C+area%5C+from%5C+separate%5C+sources.%5C+%5C+C.%5C+micholitzii%5C+occurs%5C+in%5C+the%5C+Annan%5C+Highlands%5C+in%5C+central%5C+Vietnam%5C+near%5C+the%5C+Laos%5C+border.%5C+C.%5C+bifida%5C+occurs%5C+in%5C+North%5C+Vietnam%5C%3B%5C+its%5C+distribution%5C+extends%5C+across%5C+the%5C+border%5C+into%5C+adjacent%5C+localities%5C+in%5C+Guangxi%5C+and%5C+Yunnan%5C+in%5C+China.%5C+For%5C+the%5C+comparability%5C+between%5C+them%2Ctheywere%5C+considered%5C+as%5C+the%5C+same%5C+species%5C+C.%5C+micholitzii%5C+by%5C+many%5C+academicians.%5C+The%5C+cpDNA%5C+sequences%5C+from%5C+11%5C+populations%5C+showed%5C+that%5C+these%5C+very%5C+controversial%5C+species%2C%5C+C.%5C+micholitzii%5C+and%5C+C.%5C+bifida%2C%5C+is%5C+paraphyletic%5C+and%5C+should%5C+belong%5C+to%5C+the%5C+same%5C+species%5C+C.%5C+micholitzii.%5C+AMOVA%5C+analysis%5C+showed%5C+that%5C+the%5C+component%5C+of%5C+among%5C-population%5C+within%5C+region%5C%2Fspecies%5C+%5C%2876.46%25%5C%29%5C+was%5C+unexpectedly%5C+larger%5C+than%5C+the%5C+among%5C-species%5C%2Fregion%5C+component%5C+%5C%2814.97%25%5C%29%2C%5C+which%5C+also%5C+indicates%5C+that%5C+there%5C+is%5C+no%5C+justification%5C+for%5C+recognizing%5C+two%5C+species%5C+as%5C+C.%5C+micholitzii%5C+and%5C+C.%5C+bifida.%5C+This%5C+hypothesis%5C+was%5C+also%5C+supported%5C+by%5C+the%5C+geological%5C+data%2C%5C+especially%5C+the%5C+neotectonic%5C+history%5C+of%5C+the%5C+indo%5C-china%5C+block%2C%5C+which%5C+started%5C+to%5C+move%5C+south%5C+since%5C+Oligocene%5C+and%5C+cause%5C+the%5C+geographic%5C+isolation%5C+of%5C+these%5C+two%5C+groups.%5C+Therefore%2C%5C+the%5C+most%5C+likely%5C+explanation%5C+to%5C+the%5C+phenotypic%5C+similarities%5C+between%5C+these%5C+two%5C+groups%5C+may%5C+be%5C+the%5C+retention%5C+of%5C+ancestral%5C+polymorphisms%5C+in%5C+the%5C+paraphyletic%5C+group%5C+due%5C+to%5C+incomplete%5C+lineage%5C+sorting.%5C+Furthermore%2C%5C+the%5C+similarities%5C+may%5C+also%5C+be%5C+ascribed%5C+to%5C+pollen%5C-mediated%5C+gene%5C+flow%5C+among%5C+geographically%5C+proximate%5C+populations%5C+and%5C%2For%5C+phenotypic%5C+convergence%5C+under%5C+similar%5C+selection%5C+schemes%5C+in%5C+the%5C+same%5C+region.%5C+C.micholitzi%5C+had%5C+the%5C+higest%5C+genetic%5C+diversity%5C+%5C%28HT%5C+%3D%5C+0.980%2C%5C%29%5C+and%5C+genetic%5C+differentiation%5C+%5C%28GST%5C+%3D%5C+0.830%2C%5C+NST%5C+%3D%5C+0.915%5C%29%5C+among%5C+the%5C+C.%5C+micholitzii%5C+complex.%5C+The%5C+high%5C+genetic%5C+diversity%5C+might%5C+be%5C+attributed%5C+to%5C+its%5C+long%5C+evolutionary%5C+history%2C%5C+highly%5C+diverse%5C+habitats.%5C+The%5C+ineffective%5C+mode%5C+of%5C+seed%5C+dispersal%5C+and%5C+dramatic%5C+neotectonic%5C+movement%5C+in%5C+the%5C+distribution%5C+range%5C+of%5C+this%5C+species%5C+could%5C+result%5C+in%5C+the%5C+high%5C+genetic%5C+differentiation.%5C+2.%5C+Phylogeographic%5C+analysis%5C+based%5C+on%5C+nuclear%5C+ribosomal%5C+sequences%2C%5C+We%5C+sequenced%5C+the%5C+nrDNA%5C+ITS%5C+in%5C+all%5C+27%5C+populations%5C+sampled%2C%5C+7%5C+haplotypes%5C+were%5C+identified%2C%5C+among%5C+which%5C+C.%5C+micholitzii%5C+had%5C+6%2C%5C+while%5C+C.%5C+multipinnata%2C%5C+C.%5C+longipetiolula%5C+and%5C+C.%5C+debaoensis%5C+shared%5C+the%5C+remaining%5C+one.%5C+Compared%5C+to%5C+chloroplast%5C+genes%2C%5C+nuclear%5C+genes%5C+had%5C+higher%5C+correlation%5C+between%5C+genetic%5C+and%5C+geographical%5C+distance%2C%5C+but%5C+lower%5C+interspecies%5C+differentiation%5C+%5C%2854.42%25%5C+vs%5C+25.24%25%5C%29.%5C+Phylogeographical%5C+structure%5C+of%5C+C.%5C+micholitzii%5C+and%5C+C.bifida%5C+based%5C+on%5C+ITS%5C+Variation%5C+was%5C+consistent%5C+with%5C+the%5C+morphology%5C+differentiation.%5C+This%5C+similar%5C+in%5C+nuclear%5C+gene%5C+should%5C+be%5C+ascribed%5C+to%5C+pollen%5C-mediated%5C+gene%5C+flow%5C+among%5C+geographically%5C+proximate%5C+populations.Long%5C-distance%5C+gene%5C+flow%5C+over%5C+the%5C+two%5C+groups%5C+was%5C+clearly%5C+interrupted%2C%5C+which%5C+brought%5C+on%5C+the%5C+nrDNA%5C+genetic%5C+differenciation%5C+between%5C+the%5C+geographically%5C+isolated%5C+groups%2C%5C+to%5C+a%5C+certain%5C+extent%5C+affected%5C+the%5C+morphological%5C+variation.%5C+3.%5C+Interspecies%5C+relationships%5C+among%5C+Cycas%5C+micholitzii%5C+complex%2C%5C+We%5C+analysed%5C+chloroplast%5C+sequence%5C+variation%5C+of%5C+the%5C+atpB%5C-rbcL%5C+and%5C+psbA%5C-trnH%5C+intergenic%5C+spacers%5C+in%5C+27%5C+populations%5C+sampled%5C+of%5C+C.%5C+micholitzii%5C+complex%2C%5C+AMOVA%5C+analysis%5C+showed%5C+that%5C+the%5C+component%5C+of%5C+among%5C-species%5C%2Fregion%5C+component%5C+%5C%2859.21%25%5C%29.%5C+However%2C%5C+phylogenic%5C+analysis%5C+showed%5C+that%5C+the%5C+haplotypes%5C+of%5C+C.%5C+micholitzii%5C+complex%5C+couldn%60t%5C+grouped%5C+into%5C+four%5C+clusters%5C+closely%5C+corresponding%5C+to%5C+the%5C+narrowly%5C+defined%5C+C.%5C+micholitzi%2C%5C+C.%5C+multipinnata%2C%5C+C.%5C+debaoensis%5C+and%5C+C.%5C+longipetiolula.%5C+We%5C+concluded%5C+that%5C+the%5C+conflict%5C+may%5C+result%5C+from%5C+several%5C+factors%5C%3A%5C+firstly%5C+incomplete%5C+lineage%5C+sorting%5C+of%5C+C.%5C+micholitzii%5C%3B%5C+secondly%5C+hybridization%5C%2Fintrogression%5C+of%5C+sympatrically%5C+cycads%2C%5C+which%5C+would%5C+be%5C+supported%5C+by%5C+evidence%5C+base%5C+on%5C+nrDNA%5C+ITS%5C+sequences%5C%3B%5C+thirdly%5C+intramolecular%5C+recombination%5C+in%5C+cpDNA%5C+of%5C+cycads%5C%3B%5C+eventually%5C+the%5C+neotectonic%5C+movement%5C+in%5C+the%5C+distribution%5C+range%5C+of%5C+this%5C+species."},{"jsname":"Dr. Plant RD Center[Y65J8322C1]","jscount":"1","jsurl":"/simple-search?field1=all&field=dc.type_filter&advanced=false&query1=China%2BBiodiversity%2BRed%2BList-Higher%2BPlants&&fq=dc.project.title_filter%3ADr.%5C+Plant%5C+RD%5C+Center%5C%5BY65J8322C1%5C%5D"},{"jsname":"lastIndexed","jscount":"2024-04-20"}],"资助项目","dc.project.title_filter")'>
Chiang Mai... [2]
''Investis... [1]
13th Five-... [1]
Aconitum c... [1]
Astilbe Bu... [1]
BRIDGE Pro... [1]
更多...
收录类别
SCI [274]
CSCD [7]
SSCI [3]
IC [1]
资助机构
National N... [9]
Chinese Ac... [5]
Yunnan Nat... [5]
National K... [4]
CAS/SAFEA ... [3]
Kunming In... [3]
更多...
×
知识图谱
KIB OpenIR
开始提交
已提交作品
待认领作品
已认领作品
未提交全文
收藏管理
QQ客服
官方微博
反馈留言
分面浏览：Document Type
当前检索式
((ALL：China Biodiversity Red List-Higher Plants))
限定条件
无
共7条，第1-7条
期刊论文
381
学位论文
88
专著
25
会议录
2
会议论文
1
其他
1
演示报告
1
