×
验证码:
换一张
忘记密码？
记住我
×
登录
中文版
|
English
中国科学院昆明植物研究所知识管理系统
Knowledge Management System of Kunming Institute of Botany,CAS
登录
注册
ALL
ORCID
题名
作者
学科领域
关键词
资助项目
文献类型
出处
收录类别
出版者
发表日期
存缴日期
学科门类
学习讨论厅
图片搜索
粘贴图片网址
首页
研究单元&专题
作者
文献类型
学科分类
知识图谱
新闻&公告
研究单元&专题
昆明植物所硕博研... [204]
作者
彭德力 [2]
张雁云 [2]
葛佳 [2]
张宪智 [1]
刘源 [1]
卢然然 [1]
更多...
文献类型
学位论文 [204]
发表日期
2022 [2]
2021 [3]
2020 [24]
2019 [10]
2018 [15]
2017 [19]
更多...
语种
中文 [145]
英语 [13]
出处
资助项目
GST, P < 0.05) were exhibited by this species. The SAMOVA revealed seven diverging groups of related chlorotypes, six of them had distinct nonoverlapping geographical ranges: one in the northeast comprising 10 populations, a second with a southeast distribution comprising 22 populations, and the remaning four groups comprising 15 populations located in the west part of the species’ range along different river valleys. The genetic clustering of populations into three regions was also supported by analysis of molecular variance, which showed that most genetic variation (82.43%) was found among these three regions. Two clusters were distinguished by both phylogenetic analysis and genealogical analysis of chlorotypes, one consisting of chlorotypes from the western region and the second consisting of those from the eastern region. Significant genetic differences between the two regions might be attributed to vicariance and restricted gene flow, and this vicariance could be explained by the physical environmental heterogeneity on each side of the Tanaka-Kaiyong Line. Following the uplift of the Tibetan Plateau, the reorganization of the major river drainages was primarily caused by river separation and capture events. These historical events could change the distribution of S. davidii from fragmented to continuous (Upper/Lower Jinshajiang and Yalongjiang/Daduhe), and from continuous to fragmented (Nujiang and Jinshajiang/Honghe). However, spatial and temporal patterns of phylogeographic divergence are strongly associated with historical disjunction rather than modern drainage connections. Moreover, the following north-south split in the eastern region and effective isolation with their genetic diversity were essentially modelled by genetic drift. The higher chlorotype richness and genetic divergence for populations in western region compared with other two regions suggests that there were multipe refugia or in situ survival of S. davidii in the Himalayan-Hengduan Mountain region. Fixation of chlorotypes in the northeastern region and near fixation in the southeastern region suggest a recent colonization of these areas. We further found that this species underwent past range expansion around 37-303 thousand years ago (kya). The southeastern populations likely experienced a demographic expansion via unidirectional gene flow along rivers, while northeastern populations underwent a more northward expansion, both from initial populations (s) (21, 22, 23) preserved on eastern refugia (Jinshajiang). This process might have been accompanied with a series of founder effects or bottlenecks making populations genetically impoverished. 3. Phylogeographic analysisbased on nuclear sequence，We sequenced the nuclear (ncpGS) region in all populations sampled, recovering 23 nuclear haplotypes. Compared to cpDNA, both NST (0.470) and GST (0.338) were relatively lower, but NST was also significantly larger than GST. 37.10% of the total variation was distributed among regions which was much lower than that shown by chlorotypes. Thus, more extensive distribution of nuclear haplotypes was exhibited across the geographical range instead of the strong population subdivision observed in chlorotypes. Similarly to the chloroplast data, we found that genetic differentiation of nDNA was positively correlated with the geographical distance, but the increase in the geographical distance between populations did not increase the genetic differentiation of nDNA as rapidly as that of cpDNA. These contrasting levels between the chloroplast and nuclear genomes of S. davidii are likely due to limited gene flow of cpDNA by seeds vs. the extensive gene flow of nDNA by wind-mediated pollen in the population history. We also determined from nuclear markers that haplotype diversity was reduced in the southeastern and northeastern regions due to the loss of rare haplotypes in western region. This reduction of gene diversity is also a signature of founder events or recent bottleneck during post-glacial colonization. However, nuclear diversity within populations remains high. This provides evidence that regionally pollen flow might be sufficiently high to blur the genetic identity of founder populations over a reasonably large spatial scale.3. Relationships among three varieties，The phylogenetic analysis identified two phylogroups of chlorotypes, corresponding to S. davidii var. davidii and var. chuansinesis. The former was distinguished by the abscence of predonminant nuclear haplotype H1 of the latter. The monophyletic group of chlorotypes in var. davidii and var. liangshanesis showed their relatively close relationship. And their genetic divergence from the third variety appears to be relative to their slight morphological difference in leaf size and the divergent environmental niche spaces they occupy. Thus, the observed differences in morphological characters between var. chuansinesis and other two varieties can be explained by the seed dispersal limitation illustrated above (as inferred by geographical separation) and by environmental heterogeneity (as inferred by precipitation or elevation) or by a combination of both. After all, the geological changes, drainage reorganization, and floristic differences following the Himalayan uplift have been suggested to affect the genetic structure of S. davidii. These results provide new insights into the phylogeographic pattern of plants in China. In addition, the unique population genetic structure found in S. davidii has provided important insights into the evolutionary history of this species. The genetic profile uncovered in this study is also critical for its conservation management. Our study has uncovered the existence of at least two ‘evolutionary significant units’ independent units within S. davidii, corresponding to var. davidii from eastern region and var. chuansinensis from western region. The conservation efforts should first focus on most western populations and on the southeastern ones exhibiting high levels of genetic diversity, while the genetically homogeneous northeastern populations located in the degraded Loess Plateau should require much greater conservation efforts.","jscount":"1","jsurl":"/simple-search?field1=all&field=dc.date.issued.year&advanced=false&fq=location.comm.id%3A1&query1=Habitat%2BConservation&&fq=dc.project.title_filter%3ASophora%5C+davidii%5C+%5C%28Franch.%5C%29%5C+Skeels%5C+is%5C+an%5C+endemic%5C+species%5C+to%5C+China%2C%5C+and%5C+widely%5C+distributed%5C+in%5C+the%5C+dry%5C+valleys%5C+of%5C+the%5C+Himalayan%5C-Hengduan%5C+Mountain%5C+Systems%2C%5C+the%5C+Yungui%5C+Plateau%2C%5C+the%5C+Qinling%5C+Mountain%2C%5C+the%5C+Loess%5C+Plateau%5C+and%5C+other%5C+places%5C+of%5C+China.%5C+Previous%5C+studies%5C+of%5C+plant%5C+phylogeography%5C+have%5C+focused%5C+mainly%5C+on%5C+some%5C+taxa%5C+from%5C+the%5C+mountainous%5C+areas%5C+of%5C+China%2C%5C+relatively%5C+few%5C+studies%5C+have%5C+been%5C+conducted%5C+on%5C+plant%5C+taxa%5C+from%5C+the%5C+river%5C+valleys.%5C+The%5C+population%5C+dynamics%5C+and%5C+evolutionary%5C+history%5C+of%5C+species%5C+in%5C+such%5C+habitat%5C+remain%5C+less%5C+unknown%2C%5C+including%5C+the%5C+factors%5C+affecting%5C+the%5C+population%5C+genetic%5C+structure%5C+and%5C+its%5C+potential%5C+refugia%5C+in%5C+glaciation.%5C+In%5C+this%5C+study%2C%5C+we%5C+first%5C+determine%5C+the%5C+chromosome%5C+number%2C%5C+ploidy%5C+and%5C+karyotype%5C+of%5C+most%5C+populations%5C+we%5C+sampled.%5C+Then%2C%5C+based%5C+on%5C+sequence%5C+data%5C+from%5C+two%5C+maternally%5C+inherited%5C+cpDNA%5C+and%5C+one%5C+biparentally%5C+inherited%5C+nuclear%5C+DNA%5C+fragments%2C%5C+our%5C+study%5C+revealed%5C+the%5C+genetic%5C+diversity%5C+and%5C+population%5C+genetic%5C+structure%5C+of%5C+S.%5C+davidii%5C+and%5C+factors%5C+affecting%5C+them.%5C+The%5C+demographic%5C+history%5C+and%5C+potential%5C+refugia%5C+of%5C+this%5C+speices%5C+were%5C+investigated%5C+and%5C+the%5C+genetic%5C+relationship%5C+among%5C+three%5C+varieties%5C+was%5C+also%5C+clarified.%5C+The%5C+main%5C+results%5C+are%5C+summarized%5C+as%5C+follows%5C%3A1.%5C+Cytogeography%EF%BC%8CThe%5C+chromosome%5C+number%5C+and%5C+karyotypes%5C+of%5C+14%5C+S.%5C+davidii%5C+populations%5C+have%5C+been%5C+studied.%5C+The%5C+results%5C+showed%5C+that%5C+the%5C+choromosome%5C+number%5C+of%5C+all%5C+the%5C+populations%5C+is%5C+2n%5C+%3D%5C+18.%5C+The%5C+interphase%5C+nuclei%5C+and%5C+prophase%5C+chromosomes%5C+of%5C+the%5C+species%5C+were%5C+found%5C+to%5C+be%5C+of%5C+the%5C+complex%5C+chromosome%5C+type%5C+and%5C+interstitial%5C+type.%5C+The%5C+results%5C+of%5C+karyotype%5C+analysis%5C+showed%5C+that%5C+7%5C+of%5C+14%5C+materials%5C+has%5C+satellites%2C%5C+and%5C+the%5C+number%5C+and%5C+position%5C+of%5C+satellites%5C+differ%5C+among%5C+populations%2C%5C+and%5C+thus%5C+revealed%5C+a%5C+series%5C+of%5C+diversified%5C+karyotypes.%5C+With%5C+most%5C+populations%5C+being%5C+of%5C+ploidy%2C%5C+cytogenetical%5C+divergence%5C+within%5C+the%5C+species%5C+lied%5C+mainly%5C+in%5C+chromosome%5C+size%5C+and%5C+structure.%5C+The%5C+fact%5C+that%5C+polyploidization%5C+did%5C+not%5C+occur%5C+very%5C+often%5C+for%5C+variations%5C+in%5C+Southwest%5C+China%5C+was%5C+against%5C+viewpoint%5C+that%5C+polyploidization%5C+level%5C+in%5C+this%5C+area%5C+is%5C+higher%5C+than%5C+that%5C+of%5C+other%5C+distribution%5C+areas%5C+due%5C+to%5C+the%5C+elevation%5C+of%5C+mountains%5C+and%5C+plateau.%5C+2.%5C+Phylogeographic%5C+analysisbased%5C+on%5C+chloroplast%5C+sequence%EF%BC%8CWe%5C+sequenced%5C+two%5C+cpDNA%5C+fragments%5C+rpl32%5C-trnL%5C%28UAG%5C%29intergenic%5C+spacer%5C+and%5C+trnH%5C-psbA%5C+spacer%5C+in%5C+40%5C+populations%5C+sampled%2C%5C+recovering%5C+22%5C+chlorotypes.%5C+The%5C+average%5C+with%5C-in%5C+population%5C+diversity%5C+%5C%28hS%5C+%3D%5C+0.171%5C%29%5C+was%5C+much%5C+lower%5C+than%5C+total%5C+genetic%5C+diversity%5C+%5C%28hT%5C+%3D%5C+0.857%5C%29.%5C+Population%5C+differentiation%5C+was%5C+high%5C+%5C%28NST%5C+%3D%5C+0.924%2C%5C+GST%5C+%3D%5C+0.801%5C%29%5C+indicating%5C+low%5C+levels%5C+of%5C+seed%5C-based%5C+gene%5C+flow%5C+and%5C+significant%5C+phylogeographical%5C+stucture%5C+%5C%28NST%5C+%3E%5C+GST%2C%5C+P%5C+%3C%5C+0.05%5C%29%5C+were%5C+exhibited%5C+by%5C+this%5C+species.%5C+The%5C+SAMOVA%5C+revealed%5C+seven%5C+diverging%5C+groups%5C+of%5C+related%5C+chlorotypes%2C%5C+six%5C+of%5C+them%5C+had%5C+distinct%5C+nonoverlapping%5C+geographical%5C+ranges%5C%3A%5C+one%5C+in%5C+the%5C+northeast%5C+comprising%5C+10%5C+populations%2C%5C+a%5C+second%5C+with%5C+a%5C+southeast%5C+distribution%5C+comprising%5C+22%5C+populations%2C%5C+and%5C+the%5C+remaning%5C+four%5C+groups%5C+comprising%5C+15%5C+populations%5C+located%5C+in%5C+the%5C+west%5C+part%5C+of%5C+the%5C+species%E2%80%99%5C+range%5C+along%5C+different%5C+river%5C+valleys.%5C+The%5C+genetic%5C+clustering%5C+of%5C+populations%5C+into%5C+three%5C+regions%5C+was%5C+also%5C+supported%5C+by%5C+analysis%5C+of%5C+molecular%5C+variance%2C%5C+which%5C+showed%5C+that%5C+most%5C+genetic%5C+variation%5C+%5C%2882.43%25%5C%29%5C+was%5C+found%5C+among%5C+these%5C+three%5C+regions.%5C+Two%5C+clusters%5C+were%5C+distinguished%5C+by%5C+both%5C+phylogenetic%5C+analysis%5C+and%5C+genealogical%5C+analysis%5C+of%5C+chlorotypes%2C%5C+one%5C+consisting%5C+of%5C+chlorotypes%5C+from%5C+the%5C+western%5C+region%5C+and%5C+the%5C+second%5C+consisting%5C+of%5C+those%5C+from%5C+the%5C+eastern%5C+region.%5C+Significant%5C+genetic%5C+differences%5C+between%5C+the%5C+two%5C+regions%5C+might%5C+be%5C+attributed%5C+to%5C+vicariance%5C+and%5C+restricted%5C+gene%5C+flow%2C%5C+and%5C+this%5C+vicariance%5C+could%5C+be%5C+explained%5C+by%5C+the%5C+physical%5C+environmental%5C+heterogeneity%5C+on%5C+each%5C+side%5C+of%5C+the%5C+Tanaka%5C-Kaiyong%5C+Line.%5C+Following%5C+the%5C+uplift%5C+of%5C+the%5C+Tibetan%5C+Plateau%2C%5C+the%5C+reorganization%5C+of%5C+the%5C+major%5C+river%5C+drainages%5C+was%5C+primarily%5C+caused%5C+by%5C+river%5C+separation%5C+and%5C+capture%5C+events.%5C+These%5C+historical%5C+events%5C+could%5C+change%5C+the%5C+distribution%5C+of%5C+S.%5C+davidii%5C+from%5C+fragmented%5C+to%5C+continuous%5C+%5C%28Upper%5C%2FLower%5C+Jinshajiang%5C+and%5C+Yalongjiang%5C%2FDaduhe%5C%29%2C%5C+and%5C+from%5C+continuous%5C+to%5C+fragmented%5C+%5C%28Nujiang%5C+and%5C+Jinshajiang%5C%2FHonghe%5C%29.%5C+However%2C%5C+spatial%5C+and%5C+temporal%5C+patterns%5C+of%5C+phylogeographic%5C+divergence%5C+are%5C+strongly%5C+associated%5C+with%5C+historical%5C+disjunction%5C+rather%5C+than%5C+modern%5C+drainage%5C+connections.%5C+Moreover%2C%5C+the%5C+following%5C+north%5C-south%5C+split%5C+in%5C+the%5C+eastern%5C+region%5C+and%5C+effective%5C+isolation%5C+with%5C+their%5C+genetic%5C+diversity%5C+were%5C+essentially%5C+modelled%5C+by%5C+genetic%5C+drift.%5C+The%5C+higher%5C+chlorotype%5C+richness%5C+and%5C+genetic%5C+divergence%5C+for%5C+populations%5C+in%5C+western%5C+region%5C+compared%5C+with%5C+other%5C+two%5C+regions%5C+suggests%5C+that%5C+there%5C+were%5C+multipe%5C+refugia%5C+or%5C+in%5C+situ%5C+survival%5C+of%5C+S.%5C+davidii%5C+in%5C+the%5C+Himalayan%5C-Hengduan%5C+Mountain%5C+region.%5C+Fixation%5C+of%5C+chlorotypes%5C+in%5C+the%5C+northeastern%5C+region%5C+and%5C+near%5C+fixation%5C+in%5C+the%5C+southeastern%5C+region%5C+suggest%5C+a%5C+recent%5C+colonization%5C+of%5C+these%5C+areas.%5C+We%5C+further%5C+found%5C+that%5C+this%5C+species%5C+underwent%5C+past%5C+range%5C+expansion%5C+around%5C+37%5C-303%5C+thousand%5C+years%5C+ago%5C+%5C%28kya%5C%29.%5C+The%5C+southeastern%5C+populations%5C+likely%5C+experienced%5C+a%5C+demographic%5C+expansion%5C+via%5C+unidirectional%5C+gene%5C+flow%5C+along%5C+rivers%2C%5C+while%5C+northeastern%5C+populations%5C+underwent%5C+a%5C+more%5C+northward%5C+expansion%2C%5C+both%5C+from%5C+initial%5C+populations%5C+%5C%28s%5C%29%5C+%5C%2821%2C%5C+22%2C%5C+23%5C%29%5C+preserved%5C+on%5C+eastern%5C+refugia%5C+%5C%28Jinshajiang%5C%29.%5C+This%5C+process%5C+might%5C+have%5C+been%5C+accompanied%5C+with%5C+a%5C+series%5C+of%5C+founder%5C+effects%5C+or%5C+bottlenecks%5C+making%5C+populations%5C+genetically%5C+impoverished.%5C+3.%5C+Phylogeographic%5C+analysisbased%5C+on%5C+nuclear%5C+sequence%EF%BC%8CWe%5C+sequenced%5C+the%5C+nuclear%5C+%5C%28ncpGS%5C%29%5C+region%5C+in%5C+all%5C+populations%5C+sampled%2C%5C+recovering%5C+23%5C+nuclear%5C+haplotypes.%5C+Compared%5C+to%5C+cpDNA%2C%5C+both%5C+NST%5C+%5C%280.470%5C%29%5C+and%5C+GST%5C+%5C%280.338%5C%29%5C+were%5C+relatively%5C+lower%2C%5C+but%5C+NST%5C+was%5C+also%5C+significantly%5C+larger%5C+than%5C+GST.%5C+37.10%25%5C+of%5C+the%5C+total%5C+variation%5C+was%5C+distributed%5C+among%5C+regions%5C+which%5C+was%5C+much%5C+lower%5C+than%5C+that%5C+shown%5C+by%5C+chlorotypes.%5C+Thus%2C%5C+more%5C+extensive%5C+distribution%5C+of%5C+nuclear%5C+haplotypes%5C+was%5C+exhibited%5C+across%5C+the%5C+geographical%5C+range%5C+instead%5C+of%5C+the%5C+strong%5C+population%5C+subdivision%5C+observed%5C+in%5C+chlorotypes.%5C+Similarly%5C+to%5C+the%5C+chloroplast%5C+data%2C%5C+we%5C+found%5C+that%5C+genetic%5C+differentiation%5C+of%5C+nDNA%5C+was%5C+positively%5C+correlated%5C+with%5C+the%5C+geographical%5C+distance%2C%5C+but%5C+the%5C+increase%5C+in%5C+the%5C+geographical%5C+distance%5C+between%5C+populations%5C+did%5C+not%5C+increase%5C+the%5C+genetic%5C+differentiation%5C+of%5C+nDNA%5C+as%5C+rapidly%5C+as%5C+that%5C+of%5C+cpDNA.%5C+These%5C+contrasting%5C+levels%5C+between%5C+the%5C+chloroplast%5C+and%5C+nuclear%5C+genomes%5C+of%5C+S.%5C+davidii%5C+are%5C+likely%5C+due%5C+to%5C+limited%5C+gene%5C+flow%5C+of%5C+cpDNA%5C+by%5C+seeds%5C+vs.%5C+the%5C+extensive%5C+gene%5C+flow%5C+of%5C+nDNA%5C+by%5C+wind%5C-mediated%5C+pollen%5C+in%5C+the%5C+population%5C+history.%5C+We%5C+also%5C+determined%5C+from%5C+nuclear%5C+markers%5C+that%5C+haplotype%5C+diversity%5C+was%5C+reduced%5C+in%5C+the%5C+southeastern%5C+and%5C+northeastern%5C+regions%5C+due%5C+to%5C+the%5C+loss%5C+of%5C+rare%5C+haplotypes%5C+in%5C+western%5C+region.%5C+This%5C+reduction%5C+of%5C+gene%5C+diversity%5C+is%5C+also%5C+a%5C+signature%5C+of%5C+founder%5C+events%5C+or%5C+recent%5C+bottleneck%5C+during%5C+post%5C-glacial%5C+colonization.%5C+However%2C%5C+nuclear%5C+diversity%5C+within%5C+populations%5C+remains%5C+high.%5C+This%5C+provides%5C+evidence%5C+that%5C+regionally%5C+pollen%5C+flow%5C+might%5C+be%5C+sufficiently%5C+high%5C+to%5C+blur%5C+the%5C+genetic%5C+identity%5C+of%5C+founder%5C+populations%5C+over%5C+a%5C+reasonably%5C+large%5C+spatial%5C+scale.3.%5C+Relationships%5C+among%5C+three%5C+varieties%EF%BC%8CThe%5C+phylogenetic%5C+analysis%5C+identified%5C+two%5C+phylogroups%5C+of%5C+chlorotypes%2C%5C+corresponding%5C+to%5C+S.%5C+davidii%5C+var.%5C+davidii%5C+and%5C+var.%5C+chuansinesis.%5C+The%5C+former%5C+was%5C+distinguished%5C+by%5C+the%5C+abscence%5C+of%5C+predonminant%5C+nuclear%5C+haplotype%5C+H1%5C+of%5C+the%5C+latter.%5C+The%5C+monophyletic%5C+group%5C+of%5C+chlorotypes%5C+in%5C+var.%5C+davidii%5C+and%5C+var.%5C+liangshanesis%5C+showed%5C+their%5C+relatively%5C+close%5C+relationship.%5C+And%5C+their%5C+genetic%5C+divergence%5C+from%5C+the%5C+third%5C+variety%5C+appears%5C+to%5C+be%5C+relative%5C+to%5C+their%5C+slight%5C+morphological%5C+difference%5C+in%5C+leaf%5C+size%5C+and%5C+the%5C+divergent%5C+environmental%5C+niche%5C+spaces%5C+they%5C+occupy.%5C+Thus%2C%5C+the%5C+observed%5C+differences%5C+in%5C+morphological%5C+characters%5C+between%5C+var.%5C+chuansinesis%5C+and%5C+other%5C+two%5C+varieties%5C+can%5C+be%5C+explained%5C+by%5C+the%5C+seed%5C+dispersal%5C+limitation%5C+illustrated%5C+above%5C+%5C%28as%5C+inferred%5C+by%5C+geographical%5C+separation%5C%29%5C+and%5C+by%5C+environmental%5C+heterogeneity%5C+%5C%28as%5C+inferred%5C+by%5C+precipitation%5C+or%5C+elevation%5C%29%5C+or%5C+by%5C+a%5C+combination%5C+of%5C+both.%5C+After%5C+all%2C%5C+the%5C+geological%5C+changes%2C%5C+drainage%5C+reorganization%2C%5C+and%5C+floristic%5C+differences%5C+following%5C+the%5C+Himalayan%5C+uplift%5C+have%5C+been%5C+suggested%5C+to%5C+affect%5C+the%5C+genetic%5C+structure%5C+of%5C+S.%5C+davidii.%5C+These%5C+results%5C+provide%5C+new%5C+insights%5C+into%5C+the%5C+phylogeographic%5C+pattern%5C+of%5C+plants%5C+in%5C+China.%5C+In%5C+addition%2C%5C+the%5C+unique%5C+population%5C+genetic%5C+structure%5C+found%5C+in%5C+S.%5C+davidii%5C+has%5C+provided%5C+important%5C+insights%5C+into%5C+the%5C+evolutionary%5C+history%5C+of%5C+this%5C+species.%5C+The%5C+genetic%5C+profile%5C+uncovered%5C+in%5C+this%5C+study%5C+is%5C+also%5C+critical%5C+for%5C+its%5C+conservation%5C+management.%5C+Our%5C+study%5C+has%5C+uncovered%5C+the%5C+existence%5C+of%5C+at%5C+least%5C+two%5C+%E2%80%98evolutionary%5C+significant%5C+units%E2%80%99%5C+independent%5C+units%5C+within%5C+S.%5C+davidii%2C%5C+corresponding%5C+to%5C+var.%5C+davidii%5C+from%5C+eastern%5C+region%5C+and%5C+var.%5C+chuansinensis%5C+from%5C+western%5C+region.%5C+The%5C+conservation%5C+efforts%5C+should%5C+first%5C+focus%5C+on%5C+most%5C+western%5C+populations%5C+and%5C+on%5C+the%5C+southeastern%5C+ones%5C+exhibiting%5C+high%5C+levels%5C+of%5C+genetic%5C+diversity%2C%5C+while%5C+the%5C+genetically%5C+homogeneous%5C+northeastern%5C+populations%5C+located%5C+in%5C+the%5C+degraded%5C+Loess%5C+Plateau%5C+should%5C+require%5C+much%5C+greater%5C+conservation%5C+efforts."},{"jsname":"The Taxus wallichiana complex represents an old relict conifer lineage that survived through the Tertiary. It is currently distributed in the mountain forests in South and Southwest China south of the Qinling Mountains. In the present study, we explored phylogeography of the complex by using two chloroplast DNA regions, one nuclear ribosomal DNA spacer region and eight microsatellite (SSR) loci. The main conclusions can be summarized as follows:1. Phylogeographic pattern based on chloroplast haplotypes，There were 11 cpDNA haplotypes identified in the T. wallichiana complex The complex showed a high level of genetic diversity and obvious genetic differentiation. The 44 sampled populations showed obvious genetic structure, which could be divided into five groups, namely the Huanan group, the Daba group, the Emei group, the Yunnan group and the Qinling group. There was extremely high genetic differentiation among groups, but not significant within group. The divergence times of the five lineages, estimated using average mutation rates of trnL-trnF, fell in the Pliocene. 2. Phylogeographic patterns based on ITS sequences，These included 38 unique ‘haplotypes’ based on ITS data. Their analysis showed that the T. wallichiana complex possessed a high genetic diversity. These populations could be divided into four groups, namely the Huanan group, the Daba/Emei group, the Yunnan group and the Qinling group. Based on all results, it appears that the major lineages constituting the T. wallichiana complex have arisen before Quaternary glaciation cycles, and may have survived isolated in different refugia. During interglacial periods some lineages appear to have come in contact and hybridizedbut other lineages merged forming populations with mixed haplotypes without signs of hybridization. The present-day phylogeographical distribution pattern of the T. wallichiana complex might thus be the result of repeated expansion / contractions of populations during interglacial / glacial cycles.3. Population genetic analysis using microsatellite (SSR) markers，Eight SSR loci were used for population genetic analysis on the T. wallichiana complex. A lower level of genetic diversity at the population level and high genetic differentiation among population was detected. The results of structure analysis were similar to those on the ITS data, dividing the populations into four groups (lineages). According to the results here, it was deduced that each of the 4 lineages of the T. wallichiana complex may possessed respective glacial refugia, and some lineages (such as the Qinling and Huanan lineage) might have survived in multiple refugia in the Quaternay glaciations. The present distribution pattern of this complex was likely influenced by the uplift of the QTP and Quaternary glaciation.","jscount":"1","jsurl":"/simple-search?field1=all&field=dc.date.issued.year&advanced=false&fq=location.comm.id%3A1&query1=Habitat%2BConservation&&fq=dc.project.title_filter%3AThe%5C+Taxus%5C+wallichiana%5C+complex%5C+represents%5C+an%5C+old%5C+relict%5C+conifer%5C+lineage%5C+that%5C+survived%5C+through%5C+the%5C+Tertiary.%5C+It%5C+is%5C+currently%5C+distributed%5C+in%5C+the%5C+mountain%5C+forests%5C+in%5C+South%5C+and%5C+Southwest%5C+China%5C+south%5C+of%5C+the%5C+Qinling%5C+Mountains.%C2%A0In%5C+the%5C+present%5C+study%2C%5C+we%5C+explored%5C+phylogeography%5C+of%5C+the%5C+complex%5C+by%5C+using%5C+two%5C+chloroplast%5C+DNA%5C+regions%2C%5C+one%5C+nuclear%5C+ribosomal%5C+DNA%5C+spacer%5C+region%5C+and%5C+eight%5C+microsatellite%5C+%5C%28SSR%5C%29%5C+loci.%5C+The%5C+main%5C+conclusions%5C+can%5C+be%5C+summarized%5C+as%5C+follows%5C%3A1.%5C+Phylogeographic%5C+pattern%5C+based%5C+on%5C+chloroplast%5C+haplotypes%EF%BC%8CThere%5C+were%5C+11%5C+cpDNA%5C+haplotypes%5C+identified%5C+in%5C+the%5C+T.%5C+wallichiana%5C+complex%5C+The%5C+complex%5C+showed%5C+a%5C+high%5C+level%5C+of%5C+genetic%5C+diversity%5C+and%5C+obvious%5C+genetic%5C+differentiation.%5C+The%5C+44%5C+sampled%5C+populations%5C+showed%5C+obvious%5C+genetic%5C+structure%2C%5C+which%5C+could%5C+be%5C+divided%5C+into%5C+five%5C+groups%2C%5C+namely%5C+the%5C+Huanan%5C+group%2C%5C+the%5C+Daba%5C+group%2C%5C+the%5C+Emei%5C+group%2C%5C+the%5C+Yunnan%5C+group%5C+and%5C+the%5C+Qinling%5C+group.%5C+There%5C+was%5C+extremely%5C+high%5C+genetic%5C+differentiation%5C+among%5C+groups%2C%5C+but%5C+not%5C+significant%5C+within%5C+group.%5C+The%5C+divergence%5C+times%5C+of%5C+the%5C+five%5C+lineages%2C%5C+estimated%5C+using%5C+average%5C+mutation%5C+rates%5C+of%5C+trnL%5C-trnF%2C%5C+fell%5C+in%5C+the%5C+Pliocene.%C2%A02.%5C+Phylogeographic%5C+patterns%5C+based%5C+on%5C+ITS%5C+sequences%EF%BC%8CThese%5C+included%5C+38%5C+unique%5C+%E2%80%98haplotypes%E2%80%99%5C+based%5C+on%5C+ITS%5C+data.%5C+Their%5C+analysis%5C+showed%5C+that%5C+the%5C+T.%5C+wallichiana%5C+complex%5C+possessed%5C+a%5C+high%5C+genetic%5C+diversity.%C2%A0These%5C+populations%5C+could%5C+be%5C+divided%5C+into%5C+four%5C+groups%2C%5C+namely%5C+the%5C+Huanan%5C+group%2C%5C+the%5C+Daba%5C%2FEmei%5C+group%2C%5C+the%5C+Yunnan%5C+group%5C+and%5C+the%5C+Qinling%5C+group.%5C+Based%5C+on%5C+all%5C+results%2C%5C+it%5C+appears%5C+that%5C+the%5C+major%5C+lineages%5C+constituting%5C+the%5C+T.%5C+wallichiana%5C+complex%5C+have%5C+arisen%5C+before%5C+Quaternary%5C+glaciation%5C+cycles%2C%5C+and%5C+may%5C+have%5C+survived%5C+isolated%5C+in%5C+different%5C+refugia.%5C+During%5C+interglacial%5C+periods%5C+some%5C+lineages%5C+appear%5C+to%5C+have%5C+come%5C+in%5C+contact%5C+and%5C+hybridizedbut%5C+other%5C+lineages%5C+merged%5C+forming%5C+populations%5C+with%5C+mixed%5C+haplotypes%5C+without%5C+signs%5C+of%5C+hybridization.%5C+The%5C+present%5C-day%5C+phylogeographical%5C+distribution%5C+pattern%5C+of%5C+the%5C+T.%5C+wallichiana%5C+complex%5C+might%5C+thus%5C+be%5C+the%5C+result%5C+of%5C+repeated%5C+expansion%5C+%5C%2F%5C+contractions%5C+of%5C+populations%5C+during%5C+interglacial%5C+%5C%2F%5C+glacial%5C+cycles.3.%5C+Population%5C+genetic%5C+analysis%5C+using%5C+microsatellite%5C+%5C%28SSR%5C%29%5C+markers%EF%BC%8CEight%5C+SSR%5C+loci%5C+were%5C+used%5C+for%5C+population%5C+genetic%5C+analysis%5C+on%5C+the%5C+T.%5C+wallichiana%5C+complex.%5C+A%5C+lower%5C+level%5C+of%5C+genetic%5C+diversity%5C+at%5C+the%5C+population%5C+level%5C+and%5C+high%5C+genetic%5C+differentiation%5C+among%5C+population%5C+was%5C+detected.%5C+The%5C+results%5C+of%5C+structure%5C+analysis%5C+were%5C+similar%5C+to%5C+those%5C+on%5C+the%5C+ITS%5C+data%2C%5C+dividing%5C+the%5C+populations%5C+into%5C+four%5C+groups%5C+%5C%28lineages%5C%29.%C2%A0According%5C+to%5C+the%5C+results%5C+here%2C%5C+it%5C+was%5C+deduced%5C+that%5C+each%5C+of%5C+the%5C+4%5C+lineages%5C+of%5C+the%5C+T.%5C+wallichiana%5C+complex%5C+may%5C+possessed%5C+respective%5C+glacial%5C+refugia%2C%5C+and%5C+some%5C+lineages%5C+%5C%28such%5C+as%5C+the%5C+Qinling%5C+and%5C+Huanan%5C+lineage%5C%29%5C+might%5C+have%5C+survived%5C+in%5C+multiple%5C+refugia%5C+in%5C+the%5C+Quaternay%5C+glaciations.%5C+The%5C+present%5C+distribution%5C+pattern%5C+of%5C+this%5C+complex%5C+was%5C+likely%5C+influenced%5C+by%5C+the%5C+uplift%5C+of%5C+the%5C+QTP%5C+and%5C+Quaternary%5C+glaciation."},{"jsname":"The genus Quercus consists of subgenera Quercus and Cyclobalanopsis and has approximately 531 species, making this the largest and most widely distributed genus within the Fagaceae family, occurring throughout temperate and subtropical montane areas of the Northern Hemisphere. The occurrence of recalcitrant (desiccation-sensitive) seeded plants is common in the genus Quercus, making it one of the key genera for understanding the physiology and the ecology of recalcitrant seeds. Due to habitat loss and poor regeneration, some populations of the genus Quercus are now declining. Moreover, the limited availability of good-quality seed may lead to its natural regeneration problems. To understand the cause of the population decline and to conserve iteffectively, knowledge on the seed/fruit biology of Quercus is necessary. Despite this, the seed/fruit biology of the Asian Quercus species is largely overlooked and the seed/fruit biology of Quercus subgenus Cyclobalanopsis,which is predominately distributed across tropical and subtropical Asia, is less well documented. To provide new data on the fruit biology of subgenus Cyclobalanopsis and to understand the fruit physiology and ecology of the genus Quercus comprehensively for a conservation aim, the germination and desiccation response of 11 species of subgenus Cyclobalanopsis (from S and SW China) and 11 species of subgenus Quercus (from both SW China and Europe) were investigated. The anatomic characteristics of the fruit coats was analysed on 9 of these species and the oil contents were quantified from 18 of these species. In addition, a study was carried out over 4 years on the fruit production of Q. schottkyana (subgenus Cyclobalanopsis) to fill the gap in knowledge. The data demonstrate that: 1. All 22 species of subgenus Cyclobalanopsis and subgenus Quercus had desiccation-sensitive (recalcitrant) fruits. For these 22 species which had fruit dry masses spanning 0.57 to 6.41 g and seed coat ratios spanning 0.15 to 0.48, there were wide differences in drying rates (0.26-4.10 %d-1). These differences were independent of fruit mass and seed coat ratio, but were related to the morphology of the fruit coat.2. The scar, composing 4% to 37% (surface area) of the whole fruit coat, was found to be the main water passage for most species. Water transferred directly and quickly through the scar. From the scar through to the pericarp and ending at the apex, there was a longitudinal passage of water flow. The anatomic characteristics of the fruit coats controlled the water flux, which furthermore introduced the wide differences in drying rates between the Quercus species.3. In comparison to species of Quercus subgenus Quercus, fruits in subgenus Cyclobalanopsis germinated faster and most had maximum germination at the highest temperature of 25°C. At lower temperatures (15°C, 20°C), germination of subgenus Cyclobalanopsis was slower and the germination percentage of most species was decreased, but germination of species in subgenus Quercus was not affected at these low temperatures. The thermal requirements for the germination of these two subgenera suggested an adaptability of these fruits to their habitats.4. Fruit oil content of subgenus Cyclobalanopsis (0.70% to 3.77%) was significantly lower than that of subgenus Quercus (1.48 to 18.01%) and across the 18 species studied, moisture content of the storage tissue (cotyledons) was negatively related to fruit oil content. These data were combined with that from the literature, resulting in a total of 57 species, and mapped against the current phylogeny for Quercus to reveal the highest fruit oil contents associated with sect. Lobatae. 5. The fruit production of Q. schottkyana varied markedly between years. Each square meter of Q. schottkyana pure forest produced 245-854 fruits but 14%-48% of them were infected by weevils (Curculio sp.). The annual production of Q. schottkyana was most likely affected by the average monthly rainfall during May and June, but the time of fruit dispersal was related to the rainfall of September and November. The infestation rates of weevils were density-dependent on the fruit production of Q. schottkyana that furthermore regulated the populations of these two species.","jscount":"1","jsurl":"/simple-search?field1=all&field=dc.date.issued.year&advanced=false&fq=location.comm.id%3A1&query1=Habitat%2BConservation&&fq=dc.project.title_filter%3AThe%5C+genus%5C+Quercus%5C+consists%5C+of%5C+subgenera%5C+Quercus%5C+and%5C+Cyclobalanopsis%5C+and%5C+has%5C+approximately%5C+531%5C+species%2C%5C+making%5C+this%5C+the%5C+largest%5C+and%5C+most%5C+widely%5C+distributed%5C+genus%5C+within%5C+the%5C+Fagaceae%5C+family%2C%5C+occurring%5C+throughout%5C+temperate%5C+and%5C+subtropical%5C+montane%5C+areas%5C+of%5C+the%5C+Northern%5C+Hemisphere.%5C+The%5C+occurrence%5C+of%5C+recalcitrant%5C+%5C%28desiccation%5C-sensitive%5C%29%5C+seeded%5C+plants%5C+is%5C+common%5C+in%5C+the%5C+genus%5C+Quercus%2C%5C+making%5C+it%5C+one%5C+of%5C+the%5C+key%5C+genera%5C+for%5C+understanding%5C+the%5C+physiology%5C+and%5C+the%5C+ecology%5C+of%5C+recalcitrant%5C+seeds.%5C+Due%5C+to%5C+habitat%5C+loss%5C+and%5C+poor%5C+regeneration%2C%5C+some%5C+populations%5C+of%5C+the%5C+genus%5C+Quercus%5C+are%5C+now%5C+declining.%5C+Moreover%2C%5C+the%5C+limited%5C+availability%5C+of%5C+good%5C-quality%5C+seed%5C+may%5C+lead%5C+to%5C+its%5C+natural%5C+regeneration%5C+problems.%5C+To%5C+understand%5C+the%5C+cause%5C+of%5C+the%5C+population%5C+decline%5C+and%5C+to%5C+conserve%5C+iteffectively%2C%5C+knowledge%5C+on%5C+the%5C+seed%5C%2Ffruit%5C+biology%5C+of%5C+Quercus%5C+is%5C+necessary.%5C+Despite%5C+this%2C%5C+the%5C+seed%5C%2Ffruit%5C+biology%5C+of%5C+the%5C+Asian%5C+Quercus%5C+species%5C+is%5C+largely%5C+overlooked%5C+and%5C+the%5C+seed%5C%2Ffruit%5C+biology%5C+of%5C+Quercus%5C+subgenus%5C+Cyclobalanopsis%2Cwhich%5C+is%5C+predominately%5C+distributed%5C+across%5C+tropical%5C+and%5C+subtropical%5C+Asia%2C%5C+is%5C+less%5C+well%5C+documented.%5C+To%5C+provide%5C+new%5C+data%5C+on%5C+the%5C+fruit%5C+biology%5C+of%5C+subgenus%5C+Cyclobalanopsis%5C+and%5C+to%5C+understand%5C+the%5C+fruit%5C+physiology%5C+and%5C+ecology%5C+of%5C+the%5C+genus%5C+Quercus%5C+comprehensively%5C+for%5C+a%5C+conservation%5C+aim%2C%5C+the%5C+germination%5C+and%5C+desiccation%5C+response%5C+of%5C+11%5C+species%5C+of%5C+subgenus%5C+Cyclobalanopsis%5C+%5C%28from%5C+S%5C+and%5C+SW%5C+China%5C%29%5C+and%5C+11%5C+species%5C+of%5C+subgenus%5C+Quercus%5C+%5C%28from%5C+both%5C+SW%5C+China%5C+and%5C+Europe%5C%29%5C+were%5C+investigated.%5C+The%5C+anatomic%5C+characteristics%5C+of%5C+the%5C+fruit%5C+coats%5C+was%5C+analysed%5C+on%5C+9%5C+of%5C+these%5C+species%5C+and%5C+the%5C+oil%5C+contents%5C+were%5C+quantified%5C+from%5C+18%5C+of%5C+these%5C+species.%5C+In%5C+addition%2C%5C+a%5C+study%5C+was%5C+carried%5C+out%5C+over%5C+4%5C+years%5C+on%5C+the%5C+fruit%5C+production%5C+of%5C+Q.%5C+schottkyana%5C+%5C%28subgenus%5C+Cyclobalanopsis%5C%29%5C+to%5C+fill%5C+the%5C+gap%5C+in%5C+knowledge.%5C+The%5C+data%5C+demonstrate%5C+that%5C%3A%5C+1.%5C+All%5C+22%5C+species%5C+of%5C+subgenus%5C+Cyclobalanopsis%5C+and%5C+subgenus%5C+Quercus%5C+had%5C+desiccation%5C-sensitive%5C+%5C%28recalcitrant%5C%29%5C+fruits.%5C+For%5C+these%5C+22%5C+species%5C+which%5C+had%5C+fruit%5C+dry%5C+masses%5C+spanning%5C+0.57%5C+to%5C+6.41%5C+g%5C+and%5C+seed%5C+coat%5C+ratios%5C+spanning%5C+0.15%5C+to%5C+0.48%2C%5C+there%5C+were%5C+wide%5C+differences%5C+in%5C+drying%5C+rates%5C+%5C%280.26%5C-4.10%5C+%25d%5C-1%5C%29.%5C+These%5C+differences%5C+were%5C+independent%5C+of%5C+fruit%5C+mass%5C+and%5C+seed%5C+coat%5C+ratio%2C%5C+but%5C+were%5C+related%5C+to%5C+the%5C+morphology%5C+of%5C+the%5C+fruit%5C+coat.2.%5C+%5C+The%5C+scar%2C%5C+composing%5C+4%25%5C+to%5C+37%25%5C+%5C%28surface%5C+area%5C%29%5C+of%5C+the%5C+whole%5C+fruit%5C+coat%2C%5C+was%5C+found%5C+to%5C+be%5C+the%5C+main%5C+water%5C+passage%5C+for%5C+most%5C+species.%5C+Water%5C+transferred%5C+directly%5C+and%5C+quickly%5C+through%5C+the%5C+scar.%5C+From%5C+the%5C+scar%5C+through%5C+to%5C+the%5C+pericarp%5C+and%5C+ending%5C+at%5C+the%5C+apex%2C%5C+there%5C+was%5C+a%5C+longitudinal%5C+passage%5C+of%5C+water%5C+flow.%5C+The%5C+anatomic%5C+characteristics%5C+of%5C+the%5C+fruit%5C+coats%5C+controlled%5C+the%5C+water%5C+flux%2C%5C+which%5C+furthermore%5C+introduced%5C+the%5C+wide%5C+differences%5C+in%5C+drying%5C+rates%5C+between%5C+the%5C+Quercus%5C+species.3.%5C+In%5C+comparison%5C+to%5C+species%5C+of%5C+Quercus%5C+subgenus%5C+Quercus%2C%5C+fruits%5C+in%5C+subgenus%5C+Cyclobalanopsis%5C+germinated%5C+faster%5C+and%5C+most%5C+had%5C+maximum%5C+germination%5C+at%5C+the%5C+highest%5C+temperature%5C+of%5C+25%C2%B0C.%5C+At%5C+lower%5C+temperatures%5C+%5C%2815%C2%B0C%2C%5C+20%C2%B0C%5C%29%2C%5C+germination%5C+of%5C+subgenus%5C+Cyclobalanopsis%5C+was%5C+slower%5C+and%5C+the%5C+germination%5C+percentage%5C+of%5C+most%5C+species%5C+was%5C+decreased%2C%5C+but%5C+germination%5C+of%5C+species%5C+in%5C+subgenus%5C+Quercus%5C+was%5C+not%5C+affected%5C+at%5C+these%5C+low%5C+temperatures.%5C+The%5C+thermal%5C+requirements%5C+for%5C+the%5C+germination%5C+of%5C+these%5C+two%5C+subgenera%5C+suggested%5C+an%5C+adaptability%5C+of%5C+these%5C+fruits%5C+to%5C+their%5C+habitats.4.%5C+%5C+Fruit%5C+oil%5C+content%5C+of%5C+subgenus%5C+Cyclobalanopsis%5C+%5C%280.70%25%5C+to%5C+3.77%25%5C%29%5C+was%5C+significantly%5C+lower%5C+than%5C+that%5C+of%5C+subgenus%5C+Quercus%5C+%5C%281.48%5C+to%5C+18.01%25%5C%29%5C+and%5C+across%5C+the%5C+18%5C+species%5C+studied%2C%5C+moisture%5C+content%5C+of%5C+the%5C+storage%5C+tissue%5C+%5C%28cotyledons%5C%29%5C+was%5C+negatively%5C+related%5C+to%5C+fruit%5C+oil%5C+content.%5C+These%5C+data%5C+were%5C+combined%5C+with%5C+that%5C+from%5C+the%5C+literature%2C%5C+resulting%5C+in%5C+a%5C+total%5C+of%5C+57%5C+species%2C%5C+and%5C+mapped%5C+against%5C+the%5C+current%5C+phylogeny%5C+for%5C+Quercus%5C+to%5C+reveal%5C+the%5C+highest%5C+fruit%5C+oil%5C+contents%5C+associated%5C+with%5C+sect.%5C+Lobatae.%5C+5.%5C+%5C+The%5C+fruit%5C+production%5C+of%5C+Q.%5C+schottkyana%5C+varied%5C+markedly%5C+between%5C+years.%5C+Each%5C+square%5C+meter%5C+of%5C+Q.%5C+schottkyana%5C+pure%5C+forest%5C+produced%5C+245%5C-854%5C+fruits%5C+but%5C+14%25%5C-48%25%5C+of%5C+them%5C+were%5C+infected%5C+by%5C+weevils%5C+%5C%28Curculio%5C+sp.%5C%29.%5C+The%5C+annual%5C+production%5C+of%5C+Q.%5C+schottkyana%5C+was%5C+most%5C+likely%5C+affected%5C+by%5C+the%5C+average%5C+monthly%5C+rainfall%5C+during%5C+May%5C+and%5C+June%2C%5C+but%5C+the%5C+time%5C+of%5C+fruit%5C+dispersal%5C+was%5C+related%5C+to%5C+the%5C+rainfall%5C+of%5C+September%5C+and%5C+November.%5C+The%5C+infestation%5C+rates%5C+of%5C+weevils%5C+were%5C+density%5C-dependent%5C+on%5C+the%5C+fruit%5C+production%5C+of%5C+Q.%5C+schottkyana%5C+that%5C+furthermore%5C+regulated%5C+the%5C+populations%5C+of%5C+these%5C+two%5C+species."},{"jsname":"Tupistra pingbianensis J. L. Huang & X. Z. Liu, is a newly described perennial herb narrowly distributed in South-east Yunnan, China. It belongs to genera Tupistra Ker Gawler(Liliaceae). It usually occurs on outcrops of bare rock, or occasionally as an epiphyte on tree trunks covered with humus and moss. T. pingbianensis is unusual in that it exhibits rarity according to three different ways of measuring rarity, i.e. it has a small geographical range, is a habitat specialist, and always has low abundance where it occurs. Because of this, T. pingbianensis has been listed as an endangered species and catalogued in the Chinese Species Red List. In order to discuss the causes of rarity of T. pingbianensis, the multidisciplinary investigations of the seed and seedling establishment, cytology, breeding system, and population genetic structure of the endangered T. pingbianensis were performed in this thesis. Besides, the corresponding conservation strategies were also proposed according to the above-mentioned. The main results are summarized as follows:1. Biological traits of T. pingbianensis,T. pingbianensis is a perennial herbaceous with a creeping rhizome, thick basal leaves, and an inflorescence that is a terminal spike. Florescence is from November to December, while fruiting occurs between November and December in the next year. Reproduction and spread also occurs clonally via rhizomes, most seeds simply fall from the mother plant and germinate where they land. It occurs on outcrops of bare rock, or occasionally as an epiphyte on tree trunks covered with humus and moss, which are naturally rare habitat. Throughout its small geographical range, T. pingbianensis occurs as discrete, small populations size. 2. Seed germination traits of T. pingbianensis,Seed morphology was observed and effects of substrates soil types, light, sowing depth on germination percentage of the species T. pingbianensis were investigated primarily. The results showed that the average seed size was (1.17±0.02) cm × (0.79±0.01) cm × (0.77±0.01) cm (length × width × thickness), per-hundred-seed-weight was about 35.03±0.12g. Among the three different substrates soil types and sowing depths, seeds of T. pingbianensis germinate best in alkalescence soil and shallow sowing depth (2cm). It could germinate in the both light and dark, but the germination rate can be accelerated by light obviously. Its seed has high germination rate not just in greenhouse, but also in the field. We considered that this is a good strategy to expand its population in the special habit.3. Karyotype evolution status of T. pingbianensis,The karyotype of total eight species in Campylandra, Tupistra and Aspidistra from China were reported. Considering Tupistra has the similar morphological character with Campylandra but resemble Aspidistra in karyotype. The results support the earlier study that Tupistra is a transition between Compylandra and Aspidistra. Besides, our results also showes that the T. pingbianensis and T. fungilliformis has higher karyotype asymmetry than other species in this genera, which means these species have higher karyotype evolution status. 4. Reproduction ecology of T. pingbianensis, The flower phenology, pollinators of T. pingbianensis were documented herein. We also examined the breeding system of T. pingbianensis and seed fitness traits to determine what forms of pollination and mating occur in this naturally rare species, and is there evidence of inbreeding depression in its populations. The results shows that the flowers opened 10-15 days, which suggest stigma and pollen can keep high vitality for a long time (10-15 days). The only pollinators observed on T. pingbianensis flowers were ants (Aphaenogaster smythiesii Forel,Formicidea), springtail (Hypogastrura sp., Hypogastruridae, Collembola) and one species of beetles (Anomala corpulenta Motsch, Rutelidae). These pollinators generally have restricted movement capacities and hence promote geitonogamy or mating between individuals in close proximity within populations. The results of out crossing index (OCI) pollination experiments in our study suggest that T. pingbianensis has an animal-pollinated, mixed selfing and outcrossing breeding systems. However, a pollination experiment also fail to detect significant inbreeding depression upon F1 fruit set, seed weight and germinate rate fitness-traits. Since naturally rare species T. pingbianensis is not seriously genetically impoverished and likely to have adapted to tolerating a high level of inbreeding early in its history. 5. Conservation genetic of T. pingbianensis, The levels and partitioning of genetic diversity were investigated in Tupistra pingbianensis. Here genetic diversity and patterns of genetic variation within and among 11 populations were analyzed using AFLP markers with 97 individuals across its whole geographical range. High levels of genetic variation were revealed both at the species level (P99 = 96.012%; Ht = 0.302) and at the population level (P99 = 51.41%; Hs = 0.224). Strong genetic differentiation among populations was also detected (FST = 0.2961; ⍬Ⅱ= 0.281), which corresponded to results reported for typical animal-pollinated, mixed selfing and outcrossing plant species. Special habitat and its life history traits may play an important role in shaping the genetic diversity and the genetic structure of this species. Based on the special habitat in T. pingbianensis, the most suitable strategy for its conservation is the protection of its habitat. Moreover, given the observed strong genetic differentiation among populations of T. pingbianensis, the preservation of genetic diversity in this species will require the protection of many populations as possible to maintain the current levels of genetic variability.","jscount":"1","jsurl":"/simple-search?field1=all&field=dc.date.issued.year&advanced=false&fq=location.comm.id%3A1&query1=Habitat%2BConservation&&fq=dc.project.title_filter%3ATupistra%5C+pingbianensis%5C+J.%5C+L.%5C+Huang%5C+%5C%26%5C+X.%5C+Z.%5C+Liu%2C%5C+is%5C+a%5C+newly%5C+described%5C+perennial%5C+herb%5C+narrowly%5C+distributed%5C+in%5C+South%5C-east%5C+Yunnan%2C%5C+China.%5C+It%5C+belongs%5C+to%5C+genera%5C+Tupistra%5C+Ker%5C+Gawler%5C%28Liliaceae%5C%29.%5C+It%5C+usually%5C+occurs%5C+on%5C+outcrops%5C+of%5C+bare%5C+rock%2C%5C+or%5C+occasionally%5C+as%5C+an%5C+epiphyte%5C+on%5C+tree%5C+trunks%5C+covered%5C+with%5C+humus%5C+and%5C+moss.%5C+T.%5C+pingbianensis%5C+is%5C+unusual%5C+in%5C+that%5C+it%5C+exhibits%5C+rarity%5C+according%5C+to%5C+three%5C+different%5C+ways%5C+of%5C+measuring%5C+rarity%2C%5C+i.e.%5C+it%5C+has%5C+a%5C+small%5C+geographical%5C+range%2C%5C+is%5C+a%5C+habitat%5C+specialist%2C%5C+and%5C+always%5C+has%5C+low%5C+abundance%5C+where%5C+it%5C+occurs.%5C+Because%5C+of%5C+this%2C%5C+T.%5C+pingbianensis%5C+has%5C+been%5C+listed%5C+as%5C+an%5C+endangered%5C+species%5C+and%5C+catalogued%5C+in%5C+the%5C+Chinese%5C+Species%5C+Red%5C+List.%5C+In%5C+order%5C+to%5C+discuss%5C+the%5C+causes%5C+of%5C+rarity%5C+of%5C+T.%5C+pingbianensis%2C%5C+the%5C+multidisciplinary%5C+investigations%5C+of%5C+the%5C+seed%5C+and%5C+seedling%5C+establishment%2C%5C+cytology%2C%5C+breeding%5C+system%2C%5C+and%5C+population%5C+genetic%5C+structure%5C+of%5C+the%5C+endangered%5C+T.%5C+pingbianensis%5C+were%5C+performed%5C+in%5C+this%5C+thesis.%5C+Besides%2C%5C+the%5C+corresponding%5C+conservation%5C+strategies%5C+were%5C+also%5C+proposed%5C+according%5C+to%5C+the%5C+above%5C-mentioned.%5C+The%5C+main%5C+results%5C+are%5C+summarized%5C+as%5C+follows%5C%3A1.%5C+Biological%5C+traits%5C+of%5C+T.%5C+pingbianensis%2CT.%5C+pingbianensis%5C+is%5C+a%5C+perennial%5C+herbaceous%5C+with%5C+a%5C+creeping%5C+rhizome%2C%5C+thick%5C+basal%5C+leaves%2C%5C+and%5C+an%5C+inflorescence%5C+that%5C+is%5C+a%5C+terminal%5C+spike.%5C+Florescence%5C+is%5C+from%5C+November%5C+to%5C+December%2C%5C+while%5C+fruiting%5C+occurs%5C+between%5C+November%5C+and%5C+December%5C+in%5C+the%5C+next%5C+year.%5C+Reproduction%5C+and%5C+spread%5C+also%5C+occurs%5C+clonally%5C+via%5C+rhizomes%2C%5C+most%5C+seeds%5C+simply%5C+fall%5C+from%5C+the%5C+mother%5C+plant%5C+and%5C+germinate%5C+where%5C+they%5C+land.%5C+It%5C+occurs%5C+on%5C+outcrops%5C+of%5C+bare%5C+rock%2C%5C+or%5C+occasionally%5C+as%5C+an%5C+epiphyte%5C+on%5C+tree%5C+trunks%5C+covered%5C+with%5C+humus%5C+and%5C+moss%2C%5C+which%5C+are%5C+naturally%5C+rare%5C+habitat.%5C+Throughout%5C+its%5C+small%5C+geographical%5C+range%2C%5C+T.%5C+pingbianensis%5C+occurs%5C+as%5C+discrete%2C%5C+small%5C+populations%5C+size.%5C+2.%5C+Seed%5C+germination%5C+traits%5C+of%5C+T.%5C+pingbianensis%2CSeed%5C+morphology%5C+was%5C+observed%5C+and%5C+effects%5C+of%5C+substrates%5C+soil%5C+types%2C%5C+light%2C%5C+sowing%5C+depth%5C+on%5C+germination%5C+percentage%5C+of%5C+the%5C+species%5C+T.%5C+pingbianensis%5C+were%5C+investigated%5C+primarily.%5C+The%5C+results%5C+showed%5C+that%5C+the%5C+average%5C+seed%5C+size%5C+was%5C+%5C%281.17%C2%B10.02%5C%29%5C+cm%5C+%C3%97%5C+%5C%280.79%C2%B10.01%5C%29%5C+cm%5C+%C3%97%5C+%5C%280.77%C2%B10.01%5C%29%5C+cm%5C+%5C%28length%5C+%C3%97%5C+width%5C+%C3%97%5C+thickness%5C%29%2C%5C+per%5C-hundred%5C-seed%5C-weight%5C+was%5C+about%5C+35.03%C2%B10.12g.%5C+Among%5C+the%5C+three%5C+different%5C+substrates%5C+soil%5C+types%5C+and%5C+sowing%5C+depths%2C%5C+seeds%5C+of%5C+T.%5C+pingbianensis%5C+germinate%5C+best%5C+in%5C+alkalescence%5C+soil%5C+and%5C+shallow%5C+sowing%5C+depth%5C+%5C%282cm%5C%29.%5C+It%5C+could%5C+germinate%5C+in%5C+the%5C+both%5C+light%5C+and%5C+dark%2C%5C+but%5C+the%5C+germination%5C+rate%5C+can%5C+be%5C+accelerated%5C+by%5C+light%5C+obviously.%5C+Its%5C+seed%5C+has%5C+high%5C+germination%5C+rate%5C+not%5C+just%5C+in%5C+greenhouse%2C%5C+but%5C+also%5C+in%5C+the%5C+field.%5C+We%5C+considered%5C+that%5C+this%5C+is%5C+a%5C+good%5C+strategy%5C+to%5C+expand%5C+its%5C+population%5C+in%5C+the%5C+special%5C+habit.3.%5C+Karyotype%5C+evolution%5C+status%5C+of%5C+T.%5C+pingbianensis%2CThe%5C+karyotype%5C+of%5C+total%5C+eight%5C+species%5C+in%5C+Campylandra%2C%5C+Tupistra%5C+and%5C+Aspidistra%5C+from%5C+China%5C+were%5C+reported.%5C+Considering%5C+Tupistra%5C+has%5C+the%5C+similar%5C+morphological%5C+character%5C+with%5C+Campylandra%5C+but%5C+resemble%5C+Aspidistra%5C+in%5C+karyotype.%5C+The%5C+results%5C+support%5C+the%5C+earlier%5C+study%5C+that%5C+Tupistra%5C+is%5C+a%5C+transition%5C+between%5C+Compylandra%5C+and%5C+Aspidistra.%5C+Besides%2C%5C+our%5C+results%5C+also%5C+showes%5C+that%5C+the%5C+T.%5C+pingbianensis%5C+and%5C+T.%5C+fungilliformis%5C+has%5C+higher%5C+karyotype%5C+asymmetry%5C+than%5C+other%5C+species%5C+in%5C+this%5C+genera%2C%5C+which%5C+means%5C+these%5C+species%5C+have%5C+higher%5C+karyotype%5C+evolution%5C+status.%5C+4.%5C+Reproduction%5C+ecology%5C+of%5C+T.%5C+pingbianensis%2C%5C+The%5C+flower%5C+phenology%2C%5C+pollinators%5C+of%5C+T.%5C+pingbianensis%5C+were%5C+documented%5C+herein.%5C+We%5C+also%5C+examined%5C+the%5C+breeding%5C+system%5C+of%5C+T.%5C+pingbianensis%5C+and%5C+seed%5C+fitness%5C+traits%5C+to%5C+determine%5C+what%5C+forms%5C+of%5C+pollination%5C+and%5C+mating%5C+occur%5C+in%5C+this%5C+naturally%5C+rare%5C+species%2C%5C+and%5C+is%5C+there%5C+evidence%5C+of%5C+inbreeding%5C+depression%5C+in%5C+its%5C+populations.%5C+The%5C+results%5C+shows%5C+that%5C+the%5C+flowers%5C+opened%5C+10%5C-15%5C+days%2C%5C+which%5C+suggest%5C+stigma%5C+and%5C+pollen%5C+can%5C+keep%5C+high%5C+vitality%5C+for%5C+a%5C+long%5C+time%5C+%5C%2810%5C-15%5C+days%5C%29.%5C+The%5C+only%5C+pollinators%5C+observed%5C+on%5C+T.%5C+pingbianensis%5C+flowers%5C+were%5C+ants%5C+%5C%28Aphaenogaster%5C+smythiesii%5C+Forel%2CFormicidea%5C%29%2C%5C+springtail%5C+%5C%28Hypogastrura%5C+sp.%2C%5C+Hypogastruridae%2C%5C+Collembola%5C%29%5C+and%5C+one%5C+species%5C+of%5C+beetles%5C+%5C%28Anomala%5C+corpulenta%5C+Motsch%2C%5C+Rutelidae%5C%29.%5C+These%5C+pollinators%5C+generally%5C+have%5C+restricted%5C+movement%5C+capacities%5C+and%5C+hence%5C+promote%5C+geitonogamy%5C+or%5C+mating%5C+between%5C+individuals%5C+in%5C+close%5C+proximity%5C+within%5C+populations.%5C+The%5C+results%5C+of%5C+out%5C+crossing%5C+index%5C+%5C%28OCI%5C%29%5C+pollination%5C+experiments%5C+in%5C+our%5C+study%5C+suggest%5C+that%5C+T.%5C+pingbianensis%5C+has%5C+an%5C+animal%5C-pollinated%2C%5C+mixed%5C+selfing%5C+and%5C+outcrossing%5C+breeding%5C+systems.%5C+However%2C%5C+a%5C+pollination%5C+experiment%5C+also%5C+fail%5C+to%5C+detect%5C+significant%5C+inbreeding%5C+depression%5C+upon%5C+F1%5C+fruit%5C+set%2C%5C+seed%5C+weight%5C+and%5C+germinate%5C+rate%5C+fitness%5C-traits.%5C+Since%5C+naturally%5C+rare%5C+species%5C+T.%5C+pingbianensis%5C+is%5C+not%5C+seriously%5C+genetically%5C+impoverished%5C+and%5C+likely%5C+to%5C+have%5C+adapted%5C+to%5C+tolerating%5C+a%5C+high%5C+level%5C+of%5C+inbreeding%5C+early%5C+in%5C+its%5C+history.%5C+5.%5C+Conservation%5C+genetic%5C+of%5C+T.%5C+pingbianensis%2C%5C+The%5C+levels%5C+and%5C+partitioning%5C+of%5C+genetic%5C+diversity%5C+were%5C+investigated%5C+in%5C+Tupistra%5C+pingbianensis.%5C+Here%5C+genetic%5C+diversity%5C+and%5C+patterns%5C+of%5C+genetic%5C+variation%5C+within%5C+and%5C+among%5C+11%5C+populations%5C+were%5C+analyzed%5C+using%5C+AFLP%5C+markers%5C+with%5C+97%5C+individuals%5C+across%5C+its%5C+whole%5C+geographical%5C+range.%5C+High%5C+levels%5C+of%5C+genetic%5C+variation%5C+were%5C+revealed%5C+both%5C+at%5C+the%5C+species%5C+level%5C+%5C%28P99%5C+%3D%5C+96.012%25%5C%3B%5C+Ht%5C+%3D%5C+0.302%5C%29%5C+and%5C+at%5C+the%5C+population%5C+level%5C+%5C%28P99%5C+%3D%5C+51.41%25%5C%3B%5C+Hs%5C+%3D%5C+0.224%5C%29.%5C+Strong%5C+genetic%5C+differentiation%5C+among%5C+populations%5C+was%5C+also%5C+detected%5C+%5C%28FST%5C+%3D%5C+0.2961%5C%3B%5C+%E2%8D%AC%E2%85%A1%3D%5C+0.281%5C%29%2C%5C+which%5C+corresponded%5C+to%5C+results%5C+reported%5C+for%5C+typical%5C+animal%5C-pollinated%2C%5C+mixed%5C+selfing%5C+and%5C+outcrossing%5C+plant%5C+species.%5C+Special%5C+habitat%5C+and%5C+its%5C+life%5C+history%5C+traits%5C+may%5C+play%5C+an%5C+important%5C+role%5C+in%5C+shaping%5C+the%5C+genetic%5C+diversity%5C+and%5C+the%5C+genetic%5C+structure%5C+of%5C+this%5C+species.%5C+Based%5C+on%5C+the%5C+special%5C+habitat%5C+in%5C+T.%5C+pingbianensis%2C%5C+the%5C+most%5C+suitable%5C+strategy%5C+for%5C+its%5C+conservation%5C+is%5C+the%5C+protection%5C+of%5C+its%5C+habitat.%5C+Moreover%2C%5C+given%5C+the%5C+observed%5C+strong%5C+genetic%5C+differentiation%5C+among%5C+populations%5C+of%5C+T.%5C+pingbianensis%2C%5C+the%5C+preservation%5C+of%5C+genetic%5C+diversity%5C+in%5C+this%5C+species%5C+will%5C+require%5C+the%5C+protection%5C+of%5C+many%5C+populations%5C+as%5C+possible%5C+to%5C+maintain%5C+the%5C+current%5C+levels%5C+of%5C+genetic%5C+variability."},{"jsname":"Until now, little data about the plant reproductive characters and ecological adaptation have been documented in the species-rich Sino-Himalaya region. Anemone rivularis (Ranunculaceae), mainly occurs in this area, and is of particular interest for its unique flower heliotropic movement and sex allocation strategy. In this study, we investigated the reproductive biology and adaptation mechanism of A. rivularis on the Yulong Snow Mountain Lijiang, northwestern Yunnan. The main results were summarized as follows: 1 Reproductive biology, The mating system, flowering phenology, floral morphology and pollination efficiency were examined in Anemone rivularis. This species is a perennial plant with hermaphroditic flowers, and its inflorescence is an acropetal cyme with protogynous flowers. In contrast to some self-incompatible species reported in Anemone, our results proved that A. rivularis was self-compatible. The seed set under natural pollination was more than 70%, indicating that there was no pollen limitation. Meanwhile, the seed set of artificial-cross-pollinated flowers was significantly higher than that of artificial-self-pollinated flowers, suggesting that the mixed mating system of A. rivularis was based on cross-pollination, and the results also supported a favor of outcrossing reproductive strategy for perennial herbs as some previous reports. Clearly, the reproductive strategy of A. rivularis prefer to cross-pollination in the alpine Sino-Himalayan region, in order to improve the reproductive fitness. 2 Flower heliotropism, The flower heliotropic movement mechanism, influences and adaptive significance were investigated in Anemone rivularis. The results indicated that under natural conditions, a treatment of pistils and stamens removal, flowers of A. rivularis retained accurately sun-tracking behavior through daytime, and the petals were found to close in the evening; but flowers would lose heliotropic movement if tepals were removed, with peduncles keeping a vertical orientation. This indicated that the tepals were crucial for heliotropic behavior. The flower heliotropism of A. rivularis was sensitive to blue light frequencies rather than red frequencies, suggesting that the light signal must be received by tepals, which driving the peduncles to bend due to differential cell elongation along the two sides of peduncle. Furthermore, there was a close relationship between diurnal heliotropic movements and temperature of flower interior in A. rivularis. Flowers with tepals could provide a relatively narrow range of temperatures, in comparison with flowers lacking tepals, in order to maintain reproductive organs in functional floral temperature range. Our study demonstrated that both the development of pistils and stamens and the visiting of insects could benefit from flower heliotropism in A. rivularis.3 Sex allocation, Floral traits, male and female functions, reproductive fitness, and sex allocation hypotheses were assessed in intra-inflorescence of Anemone rivularis. Though the inflorescence showed an acropetal flower-opening sequence as well as in many flowering species (early flowers are proximal and late flowers are distal), it engaged different sex allocation strategy. Our observations documented that the late-opening flowers of each inflorescence produce significantly more ovules and fewer pollen grains compared to early-opening flowers, and the pollen:ovule ratio (P:O) declined obviously from primary flower position to tertiary flower position, suggesting that later flowers would tend to favor female-bias investment. The nature-pollinating seed set among flower positions was constant, and there was no resource trade-off between flower size and sexual organs in this species, and the first-removal treatment did not lead to a significant increase in seed set of flowers in the later position. Thus, early-opening flower may not represent a significant competitor for resources with late-opening flowers on the same inflorescence, suggesting that the pattern of floral design and floral display may be determined prior to flowering and is inalterable by resources during flowering. So the female-biased allocation of distal flowers in A. rivularis may be resulted from the the selection by variation in the mating environment.","jscount":"1","jsurl":"/simple-search?field1=all&field=dc.date.issued.year&advanced=false&fq=location.comm.id%3A1&query1=Habitat%2BConservation&&fq=dc.project.title_filter%3AUntil%5C+now%2C%5C+little%5C+data%5C+about%5C+the%5C+plant%5C+reproductive%5C+characters%5C+and%5C+ecological%5C+adaptation%5C+have%5C+been%5C+documented%5C+in%5C+the%5C+species%5C-rich%5C+Sino%5C-Himalaya%5C+region.%5C+Anemone%5C+rivularis%5C+%5C%28Ranunculaceae%5C%29%2C%5C+mainly%5C+occurs%5C+in%5C+this%5C+area%2C%5C+and%5C+is%5C+of%5C+particular%5C+interest%5C+for%5C+its%5C+unique%5C+flower%5C+heliotropic%5C+movement%5C+and%5C+sex%5C+allocation%5C+strategy.%5C+In%5C+this%5C+study%2C%5C+we%5C+investigated%5C+the%5C+reproductive%5C+biology%5C+and%5C+adaptation%5C+mechanism%5C+of%5C+A.%5C+rivularis%5C+on%5C+the%5C+Yulong%5C+Snow%5C+Mountain%5C+Lijiang%2C%5C+northwestern%5C+Yunnan.%5C+The%5C+main%5C+results%5C+were%5C+summarized%5C+as%5C+follows%5C%3A%5C+1%5C+Reproductive%5C+biology%2C%5C+The%5C+mating%5C+system%2C%5C+flowering%5C+phenology%2C%5C+floral%5C+morphology%5C+and%5C+pollination%5C+efficiency%5C+were%5C+examined%5C+in%5C+Anemone%5C+rivularis.%5C+This%5C+species%5C+is%5C+a%5C+perennial%5C+plant%5C+with%5C+hermaphroditic%5C+flowers%2C%5C+and%5C+its%5C+inflorescence%5C+is%5C+an%5C+acropetal%5C+cyme%5C+with%5C+protogynous%5C+flowers.%5C+In%5C+contrast%5C+to%5C+some%5C+self%5C-incompatible%5C+species%5C+reported%5C+in%5C+Anemone%2C%5C+our%5C+results%5C+proved%5C+that%5C+A.%5C+rivularis%5C+was%5C+self%5C-compatible.%5C+The%5C+seed%5C+set%5C+under%5C+natural%5C+pollination%5C+was%5C+more%5C+than%5C+70%25%2C%5C+indicating%5C+that%5C+there%5C+was%5C+no%5C+pollen%5C+limitation.%5C+Meanwhile%2C%5C+the%5C+seed%5C+set%5C+of%5C+artificial%5C-cross%5C-pollinated%5C+flowers%5C+was%5C+significantly%5C+higher%5C+than%5C+that%5C+of%5C+artificial%5C-self%5C-pollinated%5C+flowers%2C%5C+suggesting%5C+that%5C+the%5C+mixed%5C+mating%5C+system%5C+of%5C+A.%5C+rivularis%5C+was%5C+based%5C+on%5C+cross%5C-pollination%2C%5C+and%5C+the%5C+results%5C+also%5C+supported%5C+a%5C+favor%5C+of%5C+outcrossing%5C+reproductive%5C+strategy%5C+for%5C+perennial%5C+herbs%5C+as%5C+some%5C+previous%5C+reports.%5C+Clearly%2C%5C+the%5C+reproductive%5C+strategy%5C+of%5C+A.%5C+rivularis%5C+prefer%5C+to%5C+cross%5C-pollination%5C+in%5C+the%5C+alpine%5C+Sino%5C-Himalayan%5C+region%2C%5C+in%5C+order%5C+to%5C+improve%5C+the%5C+reproductive%5C+fitness.%5C+2%5C+Flower%5C+heliotropism%2C%5C+The%5C+flower%5C+heliotropic%5C+movement%5C+mechanism%2C%5C+influences%5C+and%5C+adaptive%5C+significance%5C+were%5C+investigated%5C+in%5C+Anemone%5C+rivularis.%5C+The%5C+results%5C+indicated%5C+that%5C+under%5C+natural%5C+conditions%2C%5C+a%5C+treatment%5C+of%5C+pistils%5C+and%5C+stamens%5C+removal%2C%5C+flowers%5C+of%5C+A.%5C+rivularis%5C+retained%5C+accurately%5C+sun%5C-tracking%5C+behavior%5C+through%5C+daytime%2C%5C+and%5C+the%5C+petals%5C+were%5C+found%5C+to%5C+close%5C+in%5C+the%5C+evening%5C%3B%5C+but%5C+flowers%5C+would%5C+lose%5C+heliotropic%5C+movement%5C+if%5C+tepals%5C+were%5C+removed%2C%5C+with%5C+peduncles%5C+keeping%5C+a%5C+vertical%5C+orientation.%5C+This%5C+indicated%5C+that%5C+the%5C+tepals%5C+were%5C+crucial%5C+for%5C+heliotropic%5C+behavior.%5C+The%5C+flower%5C+heliotropism%5C+of%5C+A.%5C+rivularis%5C+was%5C+sensitive%5C+to%5C+blue%5C+light%5C+frequencies%5C+rather%5C+than%5C+red%5C+frequencies%2C%5C+suggesting%5C+that%5C+the%5C+light%5C+signal%5C+must%5C+be%5C+received%5C+by%5C+tepals%2C%5C+which%5C+driving%5C+the%5C+peduncles%5C+to%5C+bend%5C+due%5C+to%5C+differential%5C+cell%5C+elongation%5C+along%5C+the%5C+two%5C+sides%5C+of%5C+peduncle.%5C+Furthermore%2C%5C+there%5C+was%5C+a%5C+close%5C+relationship%5C+between%5C+diurnal%5C+heliotropic%5C+movements%5C+and%5C+temperature%5C+of%5C+flower%5C+interior%5C+in%5C+A.%5C+rivularis.%5C+Flowers%5C+with%5C+tepals%5C+could%5C+provide%5C+a%5C+relatively%5C+narrow%5C+range%5C+of%5C+temperatures%2C%5C+in%5C+comparison%5C+with%5C+flowers%5C+lacking%5C+tepals%2C%5C+in%5C+order%5C+to%5C+maintain%5C+reproductive%5C+organs%5C+in%5C+functional%5C+floral%5C+temperature%5C+range.%5C+Our%5C+study%5C+demonstrated%5C+that%5C+both%5C+the%5C+development%5C+of%5C+pistils%5C+and%5C+stamens%5C+and%5C+the%5C+visiting%5C+of%5C+insects%5C+could%5C+benefit%5C+from%5C+flower%5C+heliotropism%5C+in%5C+A.%5C+rivularis.3%5C+Sex%5C+allocation%2C%5C+Floral%5C+traits%2C%5C+male%5C+and%5C+female%5C+functions%2C%5C+reproductive%5C+fitness%2C%5C+and%5C+sex%5C+allocation%5C+hypotheses%5C+were%5C+assessed%5C+in%5C+intra%5C-inflorescence%5C+of%5C+Anemone%5C+rivularis.%5C+Though%5C+the%5C+inflorescence%5C+showed%5C+an%5C+acropetal%5C+flower%5C-opening%5C+sequence%5C+as%5C+well%5C+as%5C+in%5C+many%5C+flowering%5C+species%5C+%5C%28early%5C+flowers%5C+are%5C+proximal%5C+and%5C+late%5C+flowers%5C+are%5C+distal%5C%29%2C%5C+it%5C+engaged%5C+different%5C+sex%5C+allocation%5C+strategy.%5C+Our%5C+observations%5C+documented%5C+that%5C+the%5C+late%5C-opening%5C+flowers%5C+of%5C+each%5C+inflorescence%5C+produce%5C+significantly%5C+more%5C+ovules%5C+and%5C+fewer%5C+pollen%5C+grains%5C+compared%5C+to%5C+early%5C-opening%5C+flowers%2C%5C+and%5C+the%5C+pollen%5C%3Aovule%5C+ratio%5C+%5C%28P%5C%3AO%5C%29%5C+declined%5C+obviously%5C+from%5C+primary%5C+flower%5C+position%5C+to%5C+tertiary%5C+flower%5C+position%2C%5C+suggesting%5C+that%5C+later%5C+flowers%5C+would%5C+tend%5C+to%5C+favor%5C+female%5C-bias%5C+investment.%5C+The%5C+nature%5C-pollinating%5C+seed%5C+set%5C+among%5C+flower%5C+positions%5C+was%5C+constant%2C%5C+and%5C+there%5C+was%5C+no%5C+resource%5C+trade%5C-off%5C+between%5C+flower%5C+size%5C+and%5C+sexual%5C+organs%5C+in%5C+this%5C+species%2C%5C+and%5C+the%5C+first%5C-removal%5C+treatment%5C+did%5C+not%5C+lead%5C+to%5C+a%5C+significant%5C+increase%5C+in%5C+seed%5C+set%5C+of%5C+flowers%5C+in%5C+the%5C+later%5C+position.%5C+Thus%2C%5C+early%5C-opening%5C+flower%5C+may%5C+not%5C+represent%5C+a%5C+significant%5C+competitor%5C+for%5C+resources%5C+with%5C+late%5C-opening%5C+flowers%5C+on%5C+the%5C+same%5C+inflorescence%2C%5C+suggesting%5C+that%5C+the%5C+pattern%5C+of%5C+floral%5C+design%5C+and%5C+floral%5C+display%5C+may%5C+be%5C+determined%5C+prior%5C+to%5C+flowering%5C+and%5C+is%5C+inalterable%5C+by%5C+resources%5C+during%5C+flowering.%5C+So%5C+the%5C+female%5C-biased%5C+allocation%5C+of%5C+distal%5C+flowers%5C+in%5C+A.%5C+rivularis%5C+may%5C+be%5C+resulted%5C+from%5C+the%5C+the%5C+selection%5C+by%5C+variation%5C+in%5C+the%5C+mating%5C+environment."},{"jsname":"Xiaozhongdian, a town of Shangri-la County, Diqing Prefecture, was chosen as the main field site for studying the structure and characters of traditional agricultural ecosystem, by using approaches of ethnobotany, cultural anthropology and ecology. Combined with interviewing exercises in Hanpi village, Jiantang Township, this paper also discussed the impact of traditional management on the biocultural diversity. The results showed: Traditional agroecosystem in Shangri-la is an integrated system with three subsystems, which are farming, forest and grazing subsystem. The seasonal shifting grazing activity in Shangri-la, following the natural season change and the recover process of plants, is a sustainable management that protects the local biodiversity. However, along with the decay of shifting grazing tradition recently, the local Tibetans turned to use grass land and forest which is close to villages as the main grazing lands. It increased the pasturing pressure to these areas and caused productivity decreasing and biodiversity. As a symbolic part of Tibetan culture in Shangri-la, the sacred mountain culture has played a significant role in biodiversity conservation by restricting human’s behavior. The Tibetan traditional culture, indigenous knowledge and traditional ecosystem management in Shangri-la has contributed to the biodiversity conservation in this area. However, this research indicated that under the pressure of mainstream culture and market economy, traditional knowledge is vanishing; old crop land races are decreasing; diverse land use management is inclining to be single and seasonal shifting grazing tradition is fading away. The change of diversity to singularity might cause some negative impacts on the local environment and ecosystem. In this paper, advices were also given on how to combine Tibetan traditional knowledge and management experiences into sustainable development of modern agriculture. In this thesis, genetic diversity of Musella lasiocarpa (Franch.) C. Y. Wu ex H. W. Li, a plant endemic to southwest China, was also discussed through the approach of SSR markers. The wild populations of M. lasiocarpa are very rare now due to the habitat fragment and long time human’s disturbance. By conducting broad field investigation, we have found 5 wild populations near the boarder of Yunnan and Sichuan province. Seventeen microsatellite markers were isolated from M. lasiocarpa by using FIASCO method. 8 primers were selected to do the further genetic population structure and genetic diversity analysis. The results showed that genetic diversity of M. lasiocarpa’s wild populations is higher than cultivated populations. The genetic diversity difference between wild and cultivated populations is related to the different reproduction systems. Adopting the way of asexuality reproduction, the genetic basis of cultivated populations become narrow that decrease the genetic diversity. AMOVA analysis showed that 37.19% genetic differentiation is among populations and 62.81% is within population. Genetic differentiation among different populations is related to the limited gene communication. POPGENE analysis indicated that there is very little gene flow among different populations (0.4916), which is the main reason of high genetic differentiation among M. lasiocarpa populations.","jscount":"1","jsurl":"/simple-search?field1=all&field=dc.date.issued.year&advanced=false&fq=location.comm.id%3A1&query1=Habitat%2BConservation&&fq=dc.project.title_filter%3AXiaozhongdian%2C%5C+a%5C+town%5C+of%5C+Shangri%5C-la%5C+County%2C%5C+Diqing%5C+Prefecture%2C%5C+was%5C+chosen%5C+as%5C+the%5C+main%5C+field%5C+site%5C+for%5C+studying%5C+the%5C+structure%5C+and%5C+characters%5C+of%5C+traditional%5C+agricultural%5C+ecosystem%2C%5C+by%5C+using%5C+approaches%5C+of%5C+ethnobotany%2C%5C+cultural%5C+anthropology%5C+and%5C+ecology.%5C+Combined%5C+with%5C+interviewing%5C+exercises%5C+in%5C+Hanpi%5C+village%2C%5C+Jiantang%5C+Township%2C%5C+this%5C+paper%5C+also%5C+discussed%5C+the%5C+impact%5C+of%5C+traditional%5C+management%5C+on%5C+the%5C+biocultural%5C+diversity.%5C+The%5C+results%5C+showed%5C%3A%5C+Traditional%5C+agroecosystem%5C+in%5C+Shangri%5C-la%5C+is%5C+an%5C+integrated%5C+system%5C+with%5C+three%5C+subsystems%2C%5C+which%5C+are%5C+farming%2C%5C+forest%5C+and%5C+grazing%5C+subsystem.%5C+The%5C+seasonal%5C+shifting%5C+grazing%5C+activity%5C+in%5C+Shangri%5C-la%2C%5C+following%5C+the%5C+natural%5C+season%5C+change%5C+and%5C+the%5C+recover%5C+process%5C+of%5C+plants%2C%5C+is%5C+a%5C+sustainable%5C+management%5C+that%5C+protects%5C+the%5C+local%5C+biodiversity.%5C+However%2C%5C+along%5C+with%5C+the%5C+decay%5C+of%5C+shifting%5C+grazing%5C+tradition%5C+recently%2C%5C+the%5C+local%5C+Tibetans%5C+turned%5C+to%5C+use%5C+grass%5C+land%5C+and%5C+forest%5C+which%5C+is%5C+close%5C+to%5C+villages%5C+as%5C+the%5C+main%5C+grazing%5C+lands.%5C+It%5C+increased%5C+the%5C+pasturing%5C+pressure%5C+to%5C+these%5C+areas%5C+and%5C+caused%5C+productivity%5C+decreasing%5C+and%5C+biodiversity.%5C+As%5C+a%5C+symbolic%5C+part%5C+of%5C+Tibetan%5C+culture%5C+in%5C+Shangri%5C-la%2C%5C+the%5C+sacred%5C+mountain%5C+culture%5C+has%5C+played%5C+a%5C+significant%5C+role%5C+in%5C+biodiversity%5C+conservation%5C+by%5C+restricting%5C+human%E2%80%99s%5C+behavior.%5C+The%5C+Tibetan%5C+traditional%5C+culture%2C%5C+indigenous%5C+knowledge%5C+and%5C+traditional%5C+ecosystem%5C+management%5C+in%5C+Shangri%5C-la%5C+has%5C+contributed%5C+to%5C+the%5C+biodiversity%5C+conservation%5C+in%5C+this%5C+area.%5C+However%2C%5C+this%5C+research%5C+indicated%5C+that%5C+under%5C+the%5C+pressure%5C+of%5C+mainstream%5C+culture%5C+and%5C+market%5C+economy%2C%5C+traditional%5C+knowledge%5C+is%5C+vanishing%5C%3B%5C+old%5C+crop%5C+land%5C+races%5C+are%5C+decreasing%5C%3B%5C+diverse%5C+land%5C+use%5C+management%5C+is%5C+inclining%5C+to%5C+be%5C+single%5C+and%5C+seasonal%5C+shifting%5C+grazing%5C+tradition%5C+is%5C+fading%5C+away.%5C+The%5C+change%5C+of%5C+diversity%5C+to%5C+singularity%5C+might%5C+cause%5C+some%5C+negative%5C+impacts%5C+on%5C+the%5C+local%5C+environment%5C+and%5C+ecosystem.%5C+In%5C+this%5C+paper%2C%5C+advices%5C+were%5C+also%5C+given%5C+on%5C+how%5C+to%5C+combine%5C+Tibetan%5C+traditional%5C+knowledge%5C+and%5C+management%5C+experiences%5C+into%5C+sustainable%5C+development%5C+of%5C+modern%5C+agriculture.%5C+In%5C+this%5C+thesis%2C%5C+genetic%5C+diversity%5C+of%5C+Musella%5C+lasiocarpa%5C+%5C%28Franch.%5C%29%5C+C.%5C+Y.%5C+Wu%5C+ex%5C+H.%5C+W.%5C+Li%2C%5C+a%5C+plant%5C+endemic%5C+to%5C+southwest%5C+China%2C%5C+was%5C+also%5C+discussed%5C+through%5C+the%5C+approach%5C+of%5C+SSR%5C+markers.%5C+The%5C+wild%5C+populations%5C+of%5C+M.%5C+lasiocarpa%5C+are%5C+very%5C+rare%5C+now%5C+due%5C+to%5C+the%5C+habitat%5C+fragment%5C+and%5C+long%5C+time%5C+human%E2%80%99s%5C+disturbance.%5C+By%5C+conducting%5C+broad%5C+field%5C+investigation%2C%5C+we%5C+have%5C+found%5C+5%5C+wild%5C+populations%5C+near%5C+the%5C+boarder%5C+of%5C+Yunnan%5C+and%5C+Sichuan%5C+province.%5C+Seventeen%5C+microsatellite%5C+markers%5C+were%5C+isolated%5C+from%5C+M.%5C+lasiocarpa%5C+by%5C+using%5C+FIASCO%5C+method.%5C+8%5C+primers%5C+were%5C+selected%5C+to%5C+do%5C+the%5C+further%5C+genetic%5C+population%5C+structure%5C+and%5C+genetic%5C+diversity%5C+analysis.%5C+The%5C+results%5C+showed%5C+that%5C+genetic%5C+diversity%5C+of%5C+M.%5C+lasiocarpa%E2%80%99s%5C+wild%5C+populations%5C+is%5C+higher%5C+than%5C+cultivated%5C+populations.%5C+The%5C+genetic%5C+diversity%5C+difference%5C+between%5C+wild%5C+and%5C+cultivated%5C+populations%5C+is%5C+related%5C+to%5C+the%5C+different%5C+reproduction%5C+systems.%5C+Adopting%5C+the%5C+way%5C+of%5C+asexuality%5C+reproduction%2C%5C+the%5C+genetic%5C+basis%5C+of%5C+cultivated%5C+populations%5C+become%5C+narrow%5C+that%5C+decrease%5C+the%5C+genetic%5C+diversity.%5C+AMOVA%5C+analysis%5C+showed%5C+that%5C+37.19%25%5C+genetic%5C+differentiation%5C+is%5C+among%5C+populations%5C+and%5C+62.81%25%5C+is%5C+within%5C+population.%5C+Genetic%5C+differentiation%5C+among%5C+different%5C+populations%5C+is%5C+related%5C+to%5C+the%5C+limited%5C+gene%5C+communication.%5C+POPGENE%5C+analysis%5C+indicated%5C+that%5C+there%5C+is%5C+very%5C+little%5C+gene%5C+flow%5C+among%5C+different%5C+populations%5C+%5C%280.4916%5C%29%2C%5C+which%5C+is%5C+the%5C+main%5C+reason%5C+of%5C+high%5C+genetic%5C+differentiation%5C+among%5C+M.%5C+lasiocarpa%5C+populations."},{"jsname":"lastIndexed","jscount":"2024-05-15"}],"资助项目","dc.project.title_filter")'>
Aconitum c... [1]
Craigia yu... [1]
Cyatheacea... [1]
Cycas mich... [1]
Flower sce... [1]
Natural hy... [1]
更多...
收录类别
资助机构
×
知识图谱
KIB OpenIR
开始提交
已提交作品
待认领作品
已认领作品
未提交全文
收藏管理
QQ客服
官方微博
反馈留言
分面浏览：发表日期
当前检索式
((ALL：Habitat Conservation))
限定条件
((专题：昆明植物所硕博研究生毕业学位论文))
共20条，第1-20条
2022
2
2021
3
2020
24
2019
10
2018
15
2017
19
2016
13
2015
13
2014
10
2013
14
2012
16
2011
11
2010
17
2009
6
2008
11
2007
3
2006
5
2005
3
2004
6
2003
3
