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中国科学院昆明植物研究所知识管理系统
Knowledge Management System of Kunming Institute of Botany,CAS
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GST, p < 0.01). At the regional level, Chinese and Japanese L. hodgsonii had a similar estimate of genetic diversity (China: Hd = 0.847, HT = 0.869; Japan: Hd = 0.766, HT = 0.867). Populations from China and Japan possess unique sets of haplotypes, and no haplotypes were shared between the regions. Furthermore, both the phyloegenetic and network analyses recovered the haplotypes of China and Japan as two distinct clades. Thus, we suggested the disjunct distribution of L. hodgsonii in China and Japan may present the climatic vicariant relicts of the ancient widely distributed populations. After divergence, this species within each region experienced independent evolutionary process. In China, L. hodgsonii was distributed around the Sichuan Basin. This distribution range can be divided into five regions. They were Jiajin Mountain region, E’mei Mountain region, Yunnan-Guizhou Plateau region, Wushan-Wuling Mountain region and Qinling Mountain region. Twelve haplotypes were indentified within these regions. Each region had its own specific haplotypes, which had different ancestry in the network. We deduced that Chinese L. hodgsonii might survive the LGM in multiple isolated refugia around the Sichuan Basin. In Japan, L. hodgsonii was disjunctively distributed in northern Honshu and Hokkaido. Seven haplotypes were identified within this region. However, the genetic diversity in Honshu (Hd = 0.821) was much higher than that in Hokkaido (Hd = 0.513). And all haplotypes in Hokkaido were derived from Honshu. This haplotype distribution suggested that the northern Honshu could have served as refuge in Japan. Nested clade analysis (NCA) indicated multiple forces including the vicariance and long-distance dispersal affected the disjunctive distribution among populations of L. hodgsonii in Japan.2. The phylogeography of L. tongolensis，Ligularia tongolensis was distributed along the Jinshajiang watershed, Yalongjiang watershed and Wumeng Mountain. In order to deduce the demographic history of this species, we sequenced two chloroplast DNA (cpDNA) intergenic spacers (trnQ-5’rps16, trnL-rpl32) in 140 individuals from 14 populations of three groups (Jinshajiang vs. Yalongjiang vs. Wumeng) within this species range. High levels of haplotype diversity (Hd = 0.814) and total genetic diversity (HT = 0.862) were detected at the species level, based on a total oftwelve haplotypes identified. However, the intrapopulation diversity (HS = 0.349) was low, which led to the high levels of genetic divergence (GST = 0.595, NST = 0.614, FST = 0.597). In consideration of the speciation of L. tongolensis resulting from the uplifts of the Qinghai-Tibetan Plateau (QTP), we thought the present genetic structure of L. tongolensis was shaped by the fragmentation of ancestral populations during the courses of QTP uplifts. This was further supported by the absence of IBD tests (r = –0.291, p = 0.964), which suggest that the differentiation had not occurred in accordance with the isolation by distance model. The genetic differentiation in L. tongolensis appears to be associated with historical events. Meanwhile, H2 and H5, the dominant haplotypes that located on internal nodes and deviated from extinct ancestral haplotype in the network, were detected to be shared between Jinshajiang and Yalongjiang groups. We deduced that ancestral populations of this species might have had a continuous distribution range, which was then fragmented and isolated by the following tectonic events. Finally, the ancestral polymorphism, H2 and H5, were randomly allocated in Jinshajiang watershed and Yalongjiang watershed. Meanwhile, H5 was the dominant haplotype in Jinshajiang watershed; H7 was the domiant haplotype in Yalongjiang watershed and Wumeng Mountain. This haplotype distribution pattern indicated that each group might have served as a refuge for L. tongolensis during the Quaternary Glaciation. Postglacial demographic expansion was supported by unimodal mismatch distribution and star-like phylogenies, with expansion ages of 274 ka B. P. for this 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thesis is composed of five chapters.The first chapter describes chemical constituents from the leaves and twigs of Elaeagnus lanceolata. Nineteen compounds were isolated and identified, incl-uding a new compound, isoamericanol B (1), and 18 known compounds, 6-hy-droxy-3, 4-dihydro-1-oxo-β-carboline (2), 2α-hydroxy-ursolic acid (3), 2α, 23-dih-ydroxy-ursolic acid (4), maslinic acid (5), vomifoliol (6), roseoside (7), syringa-resinol (8), clemaphenol A (9), japonica acid (10), lutein (11), trilobatin (12), 3-phenyl-1-(2'', 6''-dihydroxy-phenyl-4''-O-β-D-glucopyranosyl)-1-propanone (13), n-aringenin-7-O-β-D-glucopyranoside (14), vitexin (8-C-β-D-glucopyranosyl apigeni-n) (15), 7-O-β-D-glucopyranosyl chrysin (16), isorhamnetin 3-O-α-L-rhamnopyra-nosyl-7-O-β-D-glucopyranoside (17), kaempferol-3-O-β-D-glucopyranoside (18) a-nd kaempferol-3-O-β-D-(6''''-O-trans-p-coumaroyl) glucopyranoside (19). Compou-nds 2-17 were isolated from this plant for the first time.The second chapter describes chemical constituents from the leaves and twi-gs of Gardenia sootepensis. Seven compounds were isolated and identified, incl-uding oleanolic acid (1), coronalolide methyl ester (2), sootepin D (3), sootepi-n F (4), sootepin G (5), sootepin H (6) and sootepin I (7). 4-7 are new com-pounds.The third chapter describes chemical constituents from the fruits of Gardeni-a jasminoides. Eight compounds were isolated. These compounds were identifi-ed to be jasminodiol (1), syringaresinol (2), methyl α-D-fructofuranoside (3), cl-ethric acid (4), geniposide(5), diethylhexylphthalate (6), 5'', 7''-octadecadienoic acid, 2, 3-dihydroxypropyl ester (7) and ursolic acid (8).The forth chapter describes chemical constituents from the root of Iris pseu-dacorus. Four compounds were isolated and identified, including 5α, 8α-epidio-xyergosta-6, 22-dien-3β-ol (1), ayamenin B (2), 2-hydroxy-benzoic acid (3) and β-sitosterol (4).The fifth chapter reviewed the recent progress on seco-cycloartane triterpene studies, their structure types, distribution, and biological activities were described.","jscount":"1","jsurl":"/simple-search?field1=all&field=eperson.unique.id&advanced=false&query1=Hypericum%2BJaponicum&&fq=dc.project.title_filter%3AThis%5C+thesis%5C+is%5C+composed%5C+of%5C+five%5C+chapters.The%5C+first%5C+chapter%5C+describes%5C+chemical%5C+constituents%5C+from%5C+the%5C+leaves%5C+and%5C+twigs%5C+of%5C+Elaeagnus%5C+lanceolata.%5C+Nineteen%5C+compounds%5C+were%5C+isolated%5C+and%5C+identified%2C%5C+incl%5C-uding%5C+a%5C+new%5C+compound%2C%5C+isoamericanol%5C+B%5C+%5C%281%5C%29%2C%5C+and%5C+18%5C+known%5C+compounds%2C%5C+6%5C-hy%5C-droxy%5C-3%2C%5C+4%5C-dihydro%5C-1%5C-oxo%5C-%CE%B2%5C-carboline%5C+%5C%282%5C%29%2C%5C+2%CE%B1%5C-hydroxy%5C-ursolic%5C+acid%5C+%5C%283%5C%29%2C%5C+2%CE%B1%2C%5C+23%5C-dih%5C-ydroxy%5C-ursolic%5C+acid%5C+%5C%284%5C%29%2C%5C+maslinic%5C+acid%5C+%5C%285%5C%29%2C%5C+vomifoliol%5C+%5C%286%5C%29%2C%5C+roseoside%5C+%5C%287%5C%29%2C%5C+syringa%5C-resinol%5C+%5C%288%5C%29%2C%5C+clemaphenol%5C+A%5C+%5C%289%5C%29%2C%5C+japonica%5C+acid%5C+%5C%2810%5C%29%2C%5C+lutein%5C+%5C%2811%5C%29%2C%5C+trilobatin%5C+%5C%2812%5C%29%2C%5C+3%5C-phenyl%5C-1%5C-%5C%282%27%27%2C%5C+6%27%27%5C-dihydroxy%5C-phenyl%5C-4%27%27%5C-O%5C-%CE%B2%5C-D%5C-glucopyranosyl%5C%29%5C-1%5C-propanone%5C+%5C%2813%5C%29%2C%5C+n%5C-aringenin%5C-7%5C-O%5C-%CE%B2%5C-D%5C-glucopyranoside%5C+%5C%2814%5C%29%2C%5C+vitexin%5C+%5C%288%5C-C%5C-%CE%B2%5C-D%5C-glucopyranosyl%5C+apigeni%5C-n%5C%29%5C+%5C%2815%5C%29%2C%5C+7%5C-O%5C-%CE%B2%5C-D%5C-glucopyranosyl%5C+chrysin%5C+%5C%2816%5C%29%2C%5C+isorhamnetin%5C+3%5C-O%5C-%CE%B1%5C-L%5C-rhamnopyra%5C-nosyl%5C-7%5C-O%5C-%CE%B2%5C-D%5C-glucopyranoside%5C+%5C%2817%5C%29%2C%5C+kaempferol%5C-3%5C-O%5C-%CE%B2%5C-D%5C-glucopyranoside%5C+%5C%2818%5C%29%5C+a%5C-nd%5C+kaempferol%5C-3%5C-O%5C-%CE%B2%5C-D%5C-%5C%286%27%27%27%27%5C-O%5C-trans%5C-p%5C-coumaroyl%5C%29%5C+glucopyranoside%5C+%5C%2819%5C%29.%5C+Compou%5C-nds%5C+2%5C-17%5C+were%5C+isolated%5C+from%5C+this%5C+plant%5C+for%5C+the%5C+first%5C+time.The%5C+second%5C+chapter%5C+describes%5C+chemical%5C+constituents%5C+from%5C+the%5C+leaves%5C+and%5C+twi%5C-gs%5C+of%5C+Gardenia%5C+sootepensis.%5C+Seven%5C+compounds%5C+were%5C+isolated%5C+and%5C+identified%2C%5C+incl%5C-uding%5C+oleanolic%5C+acid%5C+%5C%281%5C%29%2C%5C+coronalolide%5C+methyl%5C+ester%5C+%5C%282%5C%29%2C%5C+sootepin%5C+D%5C+%5C%283%5C%29%2C%5C+sootepi%5C-n%5C+F%5C+%5C%284%5C%29%2C%5C+sootepin%5C+G%5C+%5C%285%5C%29%2C%5C+sootepin%5C+H%5C+%5C%286%5C%29%5C+and%5C+sootepin%5C+I%5C+%5C%287%5C%29.%5C+4%5C-7%5C+are%5C+new%5C+com%5C-pounds.The%5C+third%5C+chapter%5C+describes%5C+chemical%5C+constituents%5C+from%5C+the%5C+fruits%5C+of%5C+Gardeni%5C-a%5C+jasminoides.%5C+Eight%5C+compounds%5C+were%5C+isolated.%5C+These%5C+compounds%5C+were%5C+identifi%5C-ed%5C+to%5C+be%5C+jasminodiol%5C+%5C%281%5C%29%2C%5C+syringaresinol%5C+%5C%282%5C%29%2C%5C+methyl%5C+%CE%B1%5C-D%5C-fructofuranoside%5C+%5C%283%5C%29%2C%5C+cl%5C-ethric%5C+acid%5C+%5C%284%5C%29%2C%5C+geniposide%5C%285%5C%29%2C%5C+diethylhexylphthalate%5C+%5C%286%5C%29%2C%5C+5%27%27%2C%5C+7%27%27%5C-octadecadienoic%5C+acid%2C%5C+2%2C%5C+3%5C-dihydroxypropyl%5C+ester%5C+%5C%287%5C%29%5C+and%5C+ursolic%5C+acid%5C+%5C%288%5C%29.The%5C+forth%5C+chapter%5C+describes%5C+chemical%5C+constituents%5C+from%5C+the%5C+root%5C+of%5C+Iris%5C+pseu%5C-dacorus.%5C+Four%5C+compounds%5C+were%5C+isolated%5C+and%5C+identified%2C%5C+including%5C+5%CE%B1%2C%5C+8%CE%B1%5C-epidio%5C-xyergosta%5C-6%2C%5C+22%5C-dien%5C-3%CE%B2%5C-ol%5C+%5C%281%5C%29%2C%5C+ayamenin%5C+B%5C+%5C%282%5C%29%2C%5C+2%5C-hydroxy%5C-benzoic%5C+acid%5C+%5C%283%5C%29%5C+and%5C+%CE%B2%5C-sitosterol%5C+%5C%284%5C%29.The%5C+fifth%5C+chapter%5C+reviewed%5C+the%5C+recent%5C+progress%5C+on%5C+seco%5C-cycloartane%5C+triterpene%5C+studies%2C%5C+their%5C+structure%5C+types%2C%5C+distribution%2C%5C+and%5C+biological%5C+activities%5C+were%5C+described."},{"jsname":"lastIndexed","jscount":"2024-09-06"}],"资助项目","dc.project.title_filter")'>
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杨兴伟
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郝小江
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顾玮
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石一鸣
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王雨华
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罗晓东
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李艳
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刘海洋
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赵勤实
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冯石
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秦徐杰
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叶岩松
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