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资助项目
0.05) between wild (AR = 4.651), semi-cultivated (AR = 5.091) and cultivated (AR = 5.132) populations of C. taliensis, which suggested that the genetic background of long-lived woody plant was not easy to be changed, and there were moderate high gene flow between populations. However, there was a significant difference (P < 0.05) between wild (AR = 5.9) and cultivated (AR = 7.1) populations distributed in the same place in Yun county, Yunnan province, which may result from the hybridization and introgression of species in the tea garden and anthropogenic damages to the wild population. The hypothesis of hybrid origin of C. grandibracteata was tested by morphological and microsatellites analyses. Compared with other species, the locules in ovary of C. grandibracteata are variable, which showed a morphological intermediate and mosaic. Except one private allele, Ninety-nine percent alleles of C. grandibracteata were shared with these of C. taliensis and C. sinensis var. assamica. And C. grandibracteata was nested in the cluster of C. taliensis in the UPGMA tree. Conclusively, our results supported the hypothesis of hybrid origin of C. grandibracteata partly. The speciation of C. grandibracteata was derived from hybridization and asymmetrical introgression potentially. It is possible that C. taliensis was one of its parents, but it still needs more evidences to prove that C. sinensis var. assamica was another parent.","jscount":"1","jsurl":"/simple-search?field1=all&field=dc.type_filter&advanced=false&query1=HYBRIDS&&fq=dc.project.title_filter%3ACamellia%5C+taliensis%5C+%5C%28W.%5C+W.%5C+Smith%5C%29%5C+Melchior%2C%5C+a%5C+member%5C+of%5C+Camellia%5C+sect.%5C+Thea%2C%5C+is%5C+an%5C+indigenous%5C+species%5C+in%5C+local%5C+natural%5C+forest%5C+and%5C+has%5C+a%5C+long%5C+cultivative%5C+history%5C+in%5C+western%5C+Yunnan%5C+and%5C+its%5C+neighborhood%2C%5C+where%5C+the%5C+domestications%5C+of%5C+this%5C+species%5C+in%5C+different%5C+historical%5C+periods%5C+and%5C+in%5C+different%5C+ways%5C+can%5C+be%5C+found.%5C+C.%5C+taliensis%5C+is%5C+an%5C+important%5C+contributor%5C+to%5C+the%5C+formations%5C+of%5C+tea%5C+landraces%5C+by%5C+hybridization%5C+and%5C+introgression.%5C+In%5C+the%5C+present%5C+study%2C%5C+14%5C+microsatellite%5C+loci%5C+screened%5C+from%5C+37%5C+loci%5C+were%5C+used%5C+to%5C+explore%5C+the%5C+genetic%5C+diversity%5C+about%5C+this%5C+species%5C+with%5C+579%5C+samples%5C+from%5C+25%5C+populations%5C+%5C%2816%5C+wild%5C+populations%2C%5C+4%5C+semi%5C-cultivated%5C+populations%5C+and%5C+5%5C+cultivated%5C+populations%5C%29.%5C+At%5C+the%5C+same%5C+time%2C%5C+the%5C+potential%5C+hybrid%5C+speciation%5C+of%5C+C.%5C+grandibracteata%2C%5C+was%5C+investigated%5C+using%5C+39%5C+individuals%5C+from%5C+2%5C+populations%2C%5C+along%5C+with%5C+C.%5C+taliensis%5C+and%5C+C.%5C+sinensis%5C+var.%5C+assamica%5C+%5C%2883%5C+individuals%5C+from%5C+4%5C+populations%5C%29%5C+by%5C+the%5C+same%5C+microsatellite%5C+markers.%5C+C.%5C+taliensis%5C+had%5C+a%5C+moderate%5C+high%5C+level%5C+of%5C+genetic%5C+diversity%5C+%5C%28A%5C+%3D%5C+14.3%2C%5C+Ne%3D%5C+5.7%2C%5C+HE%5C+%3D%5C+0.666%2C%5C+I%5C+%3D%5C+1.753%2C%5C+AR%5C+%3D%5C+7.2%2C%5C+PPB%5C+%3D%5C+100%25%5C%29.%5C+This%5C+may%5C+result%5C+from%5C+several%5C+factors%5C+including%5C+K%5C-strategy%2C%5C+genetic%5C+background%2C%5C+gene%5C+flow%5C+between%5C+populations%2C%5C+hybridization%5C+and%5C+introgression%5C+among%5C+species.%5C+Between%5C+wild%5C+populations%5C+of%5C+C.%5C+taliensis%2C%5C+the%5C+gene%5C+flow%5C+was%5C+moderate%5C+high%5C+%5C%28Nm%5C+%3D%5C+1.197%5C%29%2C%5C+and%5C+genetic%5C+variation%5C+was%5C+less%5C+than%5C+20%25%5C+%5C%28GST%5C+%3D%5C+0.147%2C%5C+FST%5C+%3D%5C+0.173%5C%29%2C%5C+which%5C+was%5C+similar%5C+to%5C+other%5C+research%5C+results%5C+of%5C+long%5C-lived%5C+woody%5C+plants%2C%5C+and%5C+reflected%5C+the%5C+genetic%5C+structure%5C+of%5C+its%5C+ancestry%5C+to%5C+same%5C+extent.%5C+There%5C+was%5C+a%5C+high%5C+significant%5C+correlation%5C+between%5C+geographic%5C+distance%5C+and%5C+Nei%E2%80%99s%5C+genetic%5C+distance%5C+%5C%28r%5C+%3D%5C+0.372%2C%5C+P%5C+%3D%5C+0.001%5C%29%5C+of%5C+populations%2C%5C+which%5C+accorded%5C+with%5C+isolation%5C+by%5C+distance%5C+model.%5C+Inferring%5C+from%5C+Bayesian%5C+clustering%5C+of%5C+genotypes%2C%5C+all%5C+individuals%5C+of%5C+C.%5C+taliensis%5C+were%5C+divided%5C+into%5C+two%5C+groups%2C%5C+conflicting%5C+with%5C+the%5C+result%5C+based%5C+on%5C+Nei%E2%80%99s%5C+genetic%5C+distance%5C+and%5C+real%5C+geographic%5C+distribution%2C%5C+which%5C+suggested%5C+there%5C+were%5C+heavy%5C+and%5C+non%5C-random%5C+influences%5C+by%5C+human%5C+practices.%5C+According%5C+to%5C+allelic%5C+richness%2C%5C+there%5C+were%5C+no%5C+significant%5C+differences%5C+%5C%28P%5C+%3E%5C+0.05%5C%29%5C+between%5C+wild%5C+%5C%28AR%5C+%3D%5C+4.651%5C%29%2C%5C+semi%5C-cultivated%5C+%5C%28AR%5C+%3D%5C+5.091%5C%29%5C+and%5C+cultivated%5C+%5C%28AR%5C+%3D%5C+5.132%5C%29%5C+populations%5C+of%5C+C.%5C+taliensis%2C%5C+which%5C+suggested%5C+that%5C+the%5C+genetic%5C+background%5C+of%5C+long%5C-lived%5C+woody%5C+plant%5C+was%5C+not%5C+easy%5C+to%5C+be%5C+changed%2C%5C+and%5C+there%5C+were%5C+moderate%5C+high%5C+gene%5C+flow%5C+between%5C+populations.%5C+However%2C%5C+there%5C+was%5C+a%5C+significant%5C+difference%5C+%5C%28P%5C+%3C%5C+0.05%5C%29%5C+between%5C+wild%5C+%5C%28AR%5C+%3D%5C+5.9%5C%29%5C+and%5C+cultivated%5C+%5C%28AR%5C+%3D%5C+7.1%5C%29%5C+populations%5C+distributed%5C+in%5C+the%5C+same%5C+place%5C+in%5C+Yun%5C+county%2C%5C+Yunnan%5C+province%2C%5C+which%5C+may%5C+result%5C+from%5C+the%5C+hybridization%5C+and%5C+introgression%5C+of%5C+species%5C+in%5C+the%5C+tea%5C+garden%5C+and%5C+anthropogenic%5C+damages%5C+to%5C+the%5C+wild%5C+population.%5C+The%5C+hypothesis%5C+of%5C+hybrid%5C+origin%5C+of%5C+C.%5C+grandibracteata%5C+was%5C+tested%5C+by%5C+morphological%5C+and%5C+microsatellites%5C+analyses.%5C+Compared%5C+with%5C+other%5C+species%2C%5C+the%5C+locules%5C+in%5C+ovary%5C+of%5C+C.%5C+grandibracteata%5C+are%5C+variable%2C%5C+which%5C+showed%5C+a%5C+morphological%5C+intermediate%5C+and%5C+mosaic.%5C+Except%5C+one%5C+private%5C+allele%2C%5C+Ninety%5C-nine%5C+percent%5C+alleles%5C+of%5C+C.%5C+grandibracteata%5C+were%5C+shared%5C+with%5C+these%5C+of%5C+C.%5C+taliensis%5C+and%5C+C.%5C+sinensis%5C+var.%5C+assamica.%5C+And%5C+C.%5C+grandibracteata%5C+was%5C+nested%5C+in%5C+the%5C+cluster%5C+of%5C+C.%5C+taliensis%5C+in%5C+the%5C+UPGMA%5C+tree.%5C+Conclusively%2C%5C+our%5C+results%5C+supported%5C+the%5C+hypothesis%5C+of%5C+hybrid%5C+origin%5C+of%5C+C.%5C+grandibracteata%5C+partly.%5C+The%5C+speciation%5C+of%5C+C.%5C+grandibracteata%5C+was%5C+derived%5C+from%5C+hybridization%5C+and%5C+asymmetrical%5C+introgression%5C+potentially.%5C+It%5C+is%5C+possible%5C+that%5C+C.%5C+taliensis%5C+was%5C+one%5C+of%5C+its%5C+parents%2C%5C+but%5C+it%5C+still%5C+needs%5C+more%5C+evidences%5C+to%5C+prove%5C+that%5C+C.%5C+sinensis%5C+var.%5C+assamica%5C+was%5C+another%5C+parent."},{"jsname":"China Scholarship Council","jscount":"1","jsurl":"/simple-search?field1=all&field=dc.type_filter&advanced=false&query1=HYBRIDS&&fq=dc.project.title_filter%3AChina%5C+Scholarship%5C+Council"},{"jsname":"China Scholarship Council[201504910423]","jscount":"1","jsurl":"/simple-search?field1=all&field=dc.type_filter&advanced=false&query1=HYBRIDS&&fq=dc.project.title_filter%3AChina%5C+Scholarship%5C+Council%5C%5B201504910423%5C%5D"},{"jsname":"Chinese Academy of Sciences","jscount":"1","jsurl":"/simple-search?field1=all&field=dc.type_filter&advanced=false&query1=HYBRIDS&&fq=dc.project.title_filter%3AChinese%5C+Academy%5C+of%5C+Sciences"},{"jsname":"Cytology study can reveal important biological features of plants and answers to a certain degree in phylogeny and distribution of genetic materials and so forth. By hard working of cytologists, chromosome data of plants have been increased to a great abundance, but yet disorderly distributed in different magazines, which made researches based on the whole chromosome data of one taxon rarely launched. Scientific databases have become increasingly indispensable as researching data growing daily. As Cytological studies are booming in China, in order to fill the absence of digital and statistical data of plant chromosome researches and chromosome atlas, we started to develop a Chinese Seed Plants Chromosome Database, aiming to construct a database and start to record published chromosome data of Chinese seed plants. Based on this database, we chose the part of gymnosperms and gave a discussion to the features of its chromosomes’ evolution and variation. Cytological experiments have been applied to some important phyto-groups for phylogeny research and germplasm identification.Part I: The Chinese Seed Plants Chromosome Database and Discussion on the features of Gymnosperms chromosomes，1 The Chinese Seed Plants Chromosome Database，The frame of database was constructed by Microsoft Access 2003. 19 items of data were included in, they are: Chinese and Latin names of family, genus and species; plant pictures, mitosis metaphase and karyotype figures; morphological characteristics and distributions of the plant; chromosome numbers and basic numbers; karyotype formula; karyotype description; origin of the plant material; literature and the source of photos. In this database, data can be checked and shared easily by extracted out in species sorted interface or family sorted interface. 120 species in 29 genera and 10 families of Gymnospers have been collected and input to the database. In Angiosperms, 61 species in 10 genera of family Magnoliaceae and 80 species in 3 genera of family Theaceae have been collected and input to the database.2 Discussion on the features of evolution and variation of Gymnosperms chromosomes，By data collection of the database, we analyzed chromosome features of the group Gymnosperm. Plants of Gymnosperm had been through a long historical evolution on earth, fossil records of which originated from the late Devonian period. Once an authoritative and major classification level in the plant kingdom, most Gymnosperms have been extinct unless conifers, cycads, Ginkgo and Getales. Three main features of Gymnosperm chromosomes are: relatively large chromosome, which can be recognized from figures in the database; constant chromosome numbers, in most families of Gymnosperm the basic chromosome number keeps a certain value; comparatively low variation, karyotype under family level differs a little. The variation of chromosomes in Gymnosperm is dominated by Robertsonian changes. Contrary to common variation type in Angiosperms, the variation from high unsymmetric karyotype to low unsymmetric karyotype was found in existence in Gymnosperm.Part II: cytology research on some important phyto-groups，3 Karyomorphology of three species in the order Huerteales and their phylogenetic implications，The karyomorphology of three species in Dipentodon (Dipentodontaceae), Perrottetia (Celastraceae), and Tapiscia (Tapisciaceae), namely Dipentodon sinicus, Perrottetia racemosa, and Tapiscia sinensis, was investigated in the study. Recent molecular research has discovered close relationships among these three genera, which has led to the establishment of the order Huerteales with Perrottetia being placed in Dipentodontaceae. Herein we report the chromosome numbers of D. sinicus and P. racemosa for the first time, and present their karyotype formulas as 2n = 34 = 22sm + 12st (D. sinicus), 2n = 20 = 11m + 9sm (P. racemosa), and 2n = 30 = 22m(2SAT) + 8sm (T. sinensis). Asymmetry of their karyotypes is categorized to be Type 3B in D. sinicus, Type 2A in P. racemosa, and Type 2A in T. sinensis. Each of the species shows special cytological features. Compared with Perrottetia, Dipentodon has a different basic chromosome number, a higher karyotype asymmetry, and different karyomorphology of its interphase nuclei, mitotic prophase, and metaphase. Thus, on the basis of these results, we have reservations regarding the suggestion of placing Dipentodon and Perrottetia together in the family Dipentodontaceae.4 Genomic analyses of intergeneric hybrids between Michelia crassipes and M. calcicola by GISH，Genomic in situ hybridization (GISH) is becoming the method of choice for identifying parental chromosomes in interspecific hybrids. Interspecific F1 hybrid between Michelia crassipes and M. calcicola, tow highly ornamental species in Michelia of Magnolicaceae, has been analized by double-colored GISH with its parents’ genome as the probe. Research gave the results that the chromosome number of the F1 hybrid is 2n=38 as the same of species in Michelia and other genera in Magnoliaceae, the basic chromosome is x=19, the karyotype formula is 2n=38=32m+6sm, and the asymmetry of karyotype is 1B type. Based on chromosome data of Michelia in our database, the karyotype of this genus is featured mostly by metacentric chromosomes and submetacentric chromosomes. In Mechelia, the variation range of submetacentric chromosomes is 4 to 18 and of the karyotype asymmetry is 1A to 2B type. Both the karyotype and karyotype asymmetry type of F1 hybrid is among the variation range of Michelia. The figure of GISH showed that all the 38 chromosomes of F1 hybrid have crossing parental signals, and signal on the no.1 and no.7 chromosome showed differences, which proved that both the parental genome have been transmitted to and recombinated in F1 hybrid.","jscount":"1","jsurl":"/simple-search?field1=all&field=dc.type_filter&advanced=false&query1=HYBRIDS&&fq=dc.project.title_filter%3ACytology%5C+study%5C+can%5C+reveal%5C+important%5C+biological%5C+features%5C+of%5C+plants%5C+and%5C+answers%5C+to%5C+a%5C+certain%5C+degree%5C+in%5C+phylogeny%5C+and%5C+distribution%5C+of%5C+genetic%5C+materials%5C+and%5C+so%5C+forth.%5C+By%5C+hard%5C+working%5C+of%5C+cytologists%2C%5C+chromosome%5C+data%5C+of%5C+plants%5C+have%5C+been%5C+increased%5C+to%5C+a%5C+great%5C+abundance%2C%5C+but%5C+yet%5C+disorderly%5C+distributed%5C+in%5C+different%5C+magazines%2C%5C+which%5C+made%5C+researches%5C+based%5C+on%5C+the%5C+whole%5C+chromosome%5C+data%5C+of%5C+one%5C+taxon%5C+rarely%5C+launched.%5C+Scientific%5C+databases%5C+have%5C+become%5C+increasingly%5C+indispensable%5C+as%5C+researching%5C+data%5C+growing%5C+daily.%5C+As%5C+Cytological%5C+studies%5C+are%5C+booming%5C+in%5C+China%2C%5C+in%5C+order%5C+to%5C+fill%5C+the%5C+absence%5C+of%5C+digital%5C+and%5C+statistical%5C+data%5C+of%5C+plant%5C+chromosome%5C+researches%5C+and%5C+chromosome%5C+atlas%2C%5C+we%5C+started%5C+to%5C+develop%5C+a%5C+Chinese%5C+Seed%5C+Plants%5C+Chromosome%5C+Database%2C%5C+aiming%5C+to%5C+construct%5C+a%5C+database%5C+and%5C+start%5C+to%5C+record%5C+published%5C+chromosome%5C+data%5C+of%5C+Chinese%5C+seed%5C+plants.%5C+Based%5C+on%5C+this%5C+database%2C%5C+we%5C+chose%5C+the%5C+part%5C+of%5C+gymnosperms%5C+and%5C+gave%5C+a%5C+discussion%5C+to%5C+the%5C+features%5C+of%5C+its%5C+chromosomes%E2%80%99%5C+evolution%5C+and%5C+variation.%5C+Cytological%5C+experiments%5C+have%5C+been%5C+applied%5C+to%5C+some%5C+important%5C+phyto%5C-groups%5C+for%5C+phylogeny%5C+research%5C+and%5C+germplasm%5C+identification.Part%5C+I%5C%3A%5C+The%5C+Chinese%5C+Seed%5C+Plants%5C+Chromosome%5C+Database%5C+and%5C+Discussion%5C+on%5C+the%5C+features%5C+of%5C+Gymnosperms%5C+chromosomes%EF%BC%8C1%5C+%C2%A0The%5C+Chinese%5C+Seed%5C+Plants%5C+Chromosome%5C+Database%EF%BC%8CThe%5C+frame%5C+of%5C+database%5C+was%5C+constructed%5C+by%5C+Microsoft%5C+Access%5C+2003.%5C+19%5C+items%5C+of%5C+data%5C+were%5C+included%5C+in%2C%5C+they%5C+are%5C%3A%5C+Chinese%5C+and%5C+Latin%5C+names%5C+of%5C+family%2C%5C+genus%5C+and%5C+species%5C%3B%5C+plant%5C+pictures%2C%5C+mitosis%5C+metaphase%5C+and%5C+karyotype%5C+figures%5C%3B%5C+morphological%5C+characteristics%5C+and%5C+distributions%5C+of%5C+the%5C+plant%5C%3B%5C+chromosome%5C+numbers%5C+and%5C+basic%5C+numbers%5C%3B%5C+karyotype%5C+formula%5C%3B%5C+karyotype%5C+description%5C%3B%5C+origin%5C+of%5C+the%5C+plant%5C+material%5C%3B%5C+literature%5C+and%5C+the%5C+source%5C+of%5C+photos.%5C+In%5C+this%5C+database%2C%5C+data%5C+can%5C+be%5C+checked%5C+and%5C+shared%5C+easily%5C+by%5C+extracted%5C+out%5C+in%5C+species%5C+sorted%5C+interface%5C+or%5C+family%5C+sorted%5C+interface.%5C+120%5C+species%5C+in%5C+29%5C+genera%5C+and%5C+10%5C+families%5C+of%5C+Gymnospers%5C+have%5C+been%5C+collected%5C+and%5C+input%5C+to%5C+the%5C+database.%5C+In%5C+Angiosperms%2C%5C+61%5C+species%5C+in%5C+10%5C+genera%5C+of%5C+family%5C+Magnoliaceae%5C+and%5C+80%5C+species%5C+in%5C+3%5C+genera%5C+of%5C+family%5C+Theaceae%5C+have%5C+been%5C+collected%5C+and%5C+input%5C+to%5C+the%5C+database.2%5C+Discussion%5C+on%5C+the%5C+features%5C+of%5C+evolution%5C+and%5C+variation%5C+of%5C+Gymnosperms%5C+chromosomes%EF%BC%8CBy%5C+data%5C+collection%5C+of%5C+the%5C+database%2C%5C+we%5C+analyzed%5C+chromosome%5C+features%5C+of%5C+the%5C+group%5C+Gymnosperm.%5C+Plants%5C+of%5C+Gymnosperm%5C+had%5C+been%5C+through%5C+a%5C+long%5C+historical%5C+evolution%5C+on%5C+earth%2C%5C+fossil%5C+records%5C+of%5C+which%5C+originated%5C+from%5C+the%5C+late%5C+Devonian%5C+period.%5C+Once%5C+an%5C+authoritative%5C+and%5C+major%5C+classification%5C+level%5C+in%5C+the%5C+plant%5C+kingdom%2C%5C+most%5C+Gymnosperms%5C+have%5C+been%5C+extinct%5C+unless%5C+conifers%2C%5C+cycads%2C%5C+Ginkgo%5C+and%5C+Getales.%5C+Three%5C+main%5C+features%5C+of%5C+Gymnosperm%5C+chromosomes%5C+are%5C%3A%5C+relatively%5C+large%5C+chromosome%2C%5C+which%5C+can%5C+be%5C+recognized%5C+from%5C+figures%5C+in%5C+the%5C+database%5C%3B%5C+constant%5C+chromosome%5C+numbers%2C%5C+in%5C+most%5C+families%5C+of%5C+Gymnosperm%5C+the%5C+basic%5C+chromosome%5C+number%5C+keeps%5C+a%5C+certain%5C+value%5C%3B%5C+comparatively%5C+low%5C+variation%2C%5C+karyotype%5C+under%5C+family%5C+level%5C+differs%5C+a%5C+little.%5C+The%5C+variation%5C+of%5C+chromosomes%5C+in%5C+Gymnosperm%5C+is%5C+dominated%5C+by%5C+Robertsonian%5C+changes.%5C+Contrary%5C+to%5C+common%5C+variation%5C+type%5C+in%5C+Angiosperms%2C%5C+the%5C+variation%5C+from%5C+high%5C+unsymmetric%5C+karyotype%5C+to%5C+low%5C+unsymmetric%5C+karyotype%5C+was%5C+found%5C+in%5C+existence%5C+in%5C+Gymnosperm.Part%5C+II%5C%3A%5C+cytology%5C+research%5C+on%5C+some%5C+important%5C+phyto%5C-groups%EF%BC%8C3%5C+Karyomorphology%5C+of%5C+three%5C+species%5C+in%5C+the%5C+order%5C+Huerteales%5C+and%5C+their%5C+phylogenetic%5C+implications%EF%BC%8CThe%5C+karyomorpho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a field trip at a brule in Shangri-La, a mixed population of Ligularia Cass. was found, which including L. subspicata (Bur. et Franch.) Hand.-Mazz., L. nelumbifolia (Bur. et Franch.) Hand.-Mazz., L. tongolensis (Franch.) Hand.-Mazz., L. cymbulifera (W.W.Smith) Hand.-Mazz., L. lingiana S.W.Liu, and also some individuals morphologically intermediate between L. subspicata and L. nelumbifolia. Hence, these intermediate individuals were preliminarily assumed as natural hybrids of the two Ligularia. According to their morphology, they’re assumed to form hybrids A and B. Through careful comparison of specimens in herbarium and those we collected, the inflorescence of putative hybrid A is close to L. nelumbifolia, but the shape of laminae are intergradation of L. subspicata and L. nelumbifolia; overall morphology of putative hybrids B is similar to L. nelumbifolia, but inflorescence color is as same as L. subspicata. Compared to L. nelumbifolia (39%) and L. subspicata (36.8%), the germination rate of putative hybrid B (45.7%) slightly higher than the two; but that of hybrid A is extraordinarily low (0.3%). One possible interpretation of the low rate is hybridization. 60 individuals were collected, including putative parents, other 4 species of Ligularia nearby, putative hybrid A and B. They were all direct sequenced of four cpDNA fragments, and direct sequenced or cloning sequenced of nrDNA ITS4-5. The results support that L. nelumbifolia and L. subspicata are parents of putative hybrid A, and the majority female parent is L. subspicata, L. vellerea may also be involved in the hybridization in some degree; the nuclear sequences of putative hybrid B have no superposition, and its chloroplast DNA sequences are identical with L. nelumbifolia, so putative hybrid B could not be hybrid; and there are backcross individuals exist among the putative parent L. subspicata. NewHybrids analysis of ISSR markers indicated that, the individuals of putative hybrid A are almost L. nelumbifolia and L. subspicata F1 hybrid generation (10/11), only 1/11 possibly backcross or other forms; all individuals of hybrid B are L. nelumbifolia; except one individual of L. subspicata as backcrossed, the other parent individuals are 100% reliable. This study focused on molecular evidence, complemented by ecological, reproductive and other characteristics, we demonstrated that the morphologically intermediate individuals’ origin, and the probability of belonging to each parental or hybrid class. And concluded that L. nelumbifolia and L. subspicata are the parents of putative hybrid A, L. vellerea may also be involved in the hybridization in some degree, hybrids mainly are the first generation, a few individuals may be involved in backcross, and most probably backcross with L. subspicata according to the anthesis, while the assumption of hybrid B is not supported.","jscount":"1","jsurl":"/simple-search?field1=all&field=dc.type_filter&advanced=false&query1=HYBRIDS&&fq=dc.project.title_filter%3ADuring%5C+a%5C+field%5C+trip%5C+at%5C+a%5C+brule%5C+in%5C+Shangri%5C-La%2C%5C+a%5C+mixed%5C+population%5C+of%5C+Ligularia%5C+Cass.%5C+was%5C+found%2C%5C+which%5C+including%5C+L.%5C+subspicata%5C+%5C%28Bur.%5C+et%5C+Franch.%5C%29%5C+Hand.%5C-Mazz.%2C%5C+L.%5C+nelumbifolia%5C+%5C%28Bur.%5C+et%5C+Franch.%5C%29%5C+Hand.%5C-Mazz.%2C%5C+L.%5C+tongolensis%5C+%5C%28Franch.%5C%29%5C+Hand.%5C-Mazz.%2C%5C+L.%5C+cymbulifera%5C+%5C%28W.W.Smith%5C%29%5C+Hand.%5C-Mazz.%2C%5C+L.%5C+lingiana%5C+S.W.Liu%2C%5C+and%5C+also%5C+some%5C+individuals%5C+morphologically%5C+intermediate%5C+between%5C+L.%5C+subspicata%5C+and%5C+L.%5C+nelumbifolia.%5C+Hence%2C%5C+these%5C+intermediate%5C+individuals%5C+were%5C+preliminarily%5C+assumed%5C+as%5C+natural%5C+hybrids%5C+of%5C+the%5C+two%5C+Ligularia.%5C+According%5C+to%5C+their%5C+morphology%2C%5C+they%E2%80%99re%5C+assumed%5C+to%5C+form%5C+hybrids%5C+A%5C+and%5C+B.%5C+Through%5C+careful%5C+comparison%5C+of%5C+specimens%5C+in%5C+herbarium%5C+and%5C+those%5C+we%5C+collected%2C%5C+the%5C+inflorescence%5C+of%5C+putative%5C+hybrid%5C+A%5C+is%5C+close%5C+to%5C+L.%5C+nelumbifolia%2C%5C+but%5C+the%5C+shape%5C+of%5C+laminae%5C+are%5C+intergradation%C2%A0of%5C+L.%5C+subspicata%5C+and%5C+L.%5C+nelumbifolia%5C%3B%5C+overall%5C+morphology%5C+of%5C+putative%5C+hybrids%5C+B%5C+is%5C+similar%5C+to%5C+L.%5C+nelumbifolia%2C%5C+but%5C+inflorescence%5C+color%5C+is%5C+as%5C+same%5C+as%5C+L.%5C+subspicata.%5C+Compared%5C+to%5C+L.%5C+nelumbifolia%5C+%5C%2839%25%5C%29%5C+and%5C+L.%5C+subspicata%5C+%5C%2836.8%25%5C%29%2C%5C+the%5C+germination%5C+rate%5C+of%5C+putative%5C+hybrid%5C+B%5C+%5C%2845.7%25%5C%29%5C+slightly%5C+higher%5C+than%5C+the%5C+two%5C%3B%5C+but%5C+that%5C+of%5C+hybrid%5C+A%5C+is%5C+extraordinarily%5C+low%5C+%5C%280.3%25%5C%29.%5C+One%5C+possible%5C+interpretation%5C+of%5C+the%5C+low%5C+rate%5C+is%5C+hybridization.%5C+60%5C+individuals%5C+were%5C+collected%2C%5C+including%5C+putative%5C+parents%2C%5C+other%5C+4%5C+species%5C+of%5C+Ligularia%5C+nearby%2C%5C+putative%5C+hybrid%5C+A%5C+and%5C+B.%5C+They%5C+were%5C+all%5C+direct%5C+sequenced%5C+of%5C+four%5C+cpDNA%5C+fragments%2C%5C+and%5C+direct%5C+sequenced%5C+or%5C+cloning%5C+sequenced%5C+of%5C+nrDNA%5C+ITS4%5C-5.%5C+The%5C+results%5C+support%5C+that%5C+L.%5C+nelumbifolia%5C+and%5C+L.%5C+subspicata%5C+are%5C+parents%5C+of%5C+putative%5C+hybrid%5C+A%2C%5C+and%5C+the%5C+majority%5C+female%5C+parent%5C+is%5C+L.%5C+subspicata%2C%5C+L.%5C+vellerea%5C+may%5C+also%5C+be%5C+involved%5C+in%5C+the%5C+hybridization%5C+in%5C+some%5C+degree%5C%3B%5C+the%5C+nuclear%5C+sequences%5C+of%5C+putative%5C+hybrid%5C+B%5C+have%5C+no%5C+superposition%2C%5C+and%5C+its%5C+chloroplast%5C+DNA%5C+sequences%5C+are%5C+identical%5C+with%5C+L.%5C+nelumbifolia%2C%5C+so%5C+putative%5C+hybrid%5C+B%5C+could%5C+not%5C+be%5C+hybrid%5C%3B%5C+and%5C+there%5C+are%5C+backcross%5C+individuals%5C+exist%5C+among%5C+the%5C+putative%5C+parent%5C+L.%5C+subspicata.%5C+NewHybrids%5C+analysis%5C+of%5C+ISSR%5C+markers%5C+indicated%5C+that%2C%5C+the%5C+individuals%5C+of%5C+putative%5C+hybrid%5C+A%5C+are%5C+almost%5C+L.%5C+nelumbifolia%5C+and%5C+L.%5C+subspicata%5C+F1%5C+hybrid%5C+generation%5C+%5C%2810%5C%2F11%5C%29%2C%5C+only%5C+1%5C%2F11%5C+possibly%5C+backcross%5C+or%5C+other%5C+forms%5C%3B%5C+all%5C+individuals%5C+of%5C+hybrid%5C+B%5C+are%5C+L.%5C+nelumbifolia%5C%3B%5C+except%5C+one%5C+individual%5C+of%5C+L.%5C+subspicata%5C+as%5C+backcrossed%2C%5C+the%5C+other%5C+parent%5C+individuals%5C+are%5C+100%25%5C+reliable.%5C+This%5C+study%5C+focused%5C+on%5C+molecular%5C+evidence%2C%5C+complemented%5C+by%5C+ecological%2C%5C+reproductive%5C+and%5C+other%5C+characteristics%2C%5C+we%5C+demonstrated%5C+that%5C+the%5C+morphologically%5C+intermediate%5C+individuals%E2%80%99%5C+origin%2C%5C+and%5C+the%5C+probability%5C+of%5C+belonging%5C+to%5C+each%5C+parental%5C+or%5C+hybrid%5C+class.%5C+And%5C+concluded%5C+that%5C+L.%5C+nelumbifolia%5C+and%5C+L.%5C+subspicata%5C+are%5C+the%5C+parents%5C+of%5C+putative%5C+hybrid%5C+A%2C%5C+L.%5C+vellerea%5C+may%5C+also%5C+be%5C+involved%5C+in%5C+the%5C+hybridization%5C+in%5C+some%5C+degree%2C%5C+hybrids%5C+mainly%5C+are%5C+the%5C+first%5C+generation%2C%5C+a%5C+few%5C+individuals%5C+may%5C+be%5C+involved%5C+in%5C+backcross%2C%5C+and%5C+most%5C+probably%5C+backcross%5C+with%5C+L.%5C+subspicata%5C+according%5C+to%5C+the%5C+anthesis%2C%5C+while%5C+the%5C+assumption%5C+of%5C+hybrid%5C+B%5C+is%5C+not%5C+supported."},{"jsname":"Glory Light International Fellowship for Chinese Botanists at Missouri Botanical Garden","jscount":"1","jsurl":"/simple-search?field1=all&field=dc.type_filter&advanced=false&query1=HYBRIDS&&fq=dc.project.title_filter%3AGlory%5C+Light%5C+International%5C+Fellowship%5C+for%5C+Chinese%5C+Botanists%5C+at%5C+Missouri%5C+Botanical%5C+Garden"},{"jsname":"In order to investigate the indole alkaloids, this paper studied the chemical constituents from three plants, Gelsemium elegans (Loganiaceae), Trigonostemon thyrsoideus (Euphorbiaceae), and Buddleja Alternifolia (Loganiaceae). 67 compounds were isolated including 20 new compounds.The main work of this thesis is that investigation and evaluation of indole alkaloids in detail from G. elegans collected in Xishuanbanna, Yunnan Province of China.Phytochemical investigation on the total base fraction of G. elegans lead to harvest 51 compounds, including 18 new indole alkaloids and one new iridoid. Among them, 8 Humantenine-type alkaloids including 2 new ones; 1 new Gelselegine-type one; 14 Gelsedine-type ones, of 3 new ones; 11 Sarpagine-type ones, of 2 new ones; 6 koumine-type ones, of 4 new ones; 3 Gelsemine-type ones, and 2 yohimbane-type ones which are isolated at the first time. Moreover, it is firstly reported 4 new β-carboline alkaloids with a 3-substitution. The biosynthesis pathway of all types of alkaloids especially new compounds are proposed. At the same time, the bioassay of part of alkaloids were performed.In this paper, two unusual “chemical phenomenons” were stated and explored.Moreover, two novel daphnane diterpenes with unusual 4,6-epoxide and six totaxins and sterols were isolated from trigonostemon thyrsoideus. And then, one phenylpropanoid and five sterols were found from Buddleja Alternifolia.The review outline indole alkaloids and their anti-tumor activities.","jscount":"1","jsurl":"/simple-search?field1=all&field=dc.type_filter&advanced=false&query1=HYBRIDS&&fq=dc.project.title_filter%3AIn%5C+order%5C+to%5C+investigate%5C+the%5C+indole%5C+alkaloids%2C%5C+this%5C+paper%5C+studied%5C+the%5C+chemical%5C+constituents%5C+from%5C+three%5C+plants%2C%5C+Gelsemium%5C+elegans%5C+%5C%28Loganiaceae%5C%29%2C%5C+Trigonostemon%5C+thyrsoideus%5C+%5C%28Euphorbiaceae%5C%29%2C%5C+and%5C+Buddleja%5C+Alternifolia%5C+%5C%28Loganiaceae%5C%29.%5C+67%5C+compounds%5C+were%5C+isolated%5C+including%5C+20%5C+new%5C+compounds.The%5C+main%5C+work%5C+of%5C+this%5C+thesis%5C+is%5C+that%5C+investigation%5C+and%5C+evaluation%5C+of%5C+indole%5C+alkaloids%5C+in%5C+detail%5C+from%5C+G.%5C+elegans%5C+collected%5C+in%5C+Xishuanbanna%2C%5C+Yunnan%5C+Province%5C+of%5C+China.Phytochemical%5C+investigation%5C+on%5C+the%5C+total%5C+base%5C+fraction%5C+of%5C+G.%5C+elegans%5C+lead%5C+to%5C+harvest%5C+51%5C+compounds%2C%5C+including%5C+18%5C+new%5C+indole%5C+alkaloids%5C+and%5C+one%5C+new%5C+iridoid.%5C+Among%5C+them%2C%5C+8%5C+Humantenine%5C-type%5C+alkaloids%5C+including%5C+2%5C+new%5C+ones%5C%3B%5C+1%5C+new%5C+Gelselegine%5C-type%5C+one%5C%3B%5C+14%5C+Gelsedine%5C-type%5C+ones%2C%5C+of%5C+3%5C+new%5C+ones%5C%3B%5C+11%5C+Sarpagine%5C-type%5C+ones%2C%5C+of%5C+2%5C+new%5C+ones%5C%3B%5C+6%5C+koumine%5C-type%5C+ones%2C%5C+of%5C+4%5C+new%5C+ones%5C%3B%5C+3%5C+Gelsemine%5C-type%5C+ones%2C%5C+and%5C+2%5C+yohimbane%5C-type%5C+ones%5C+which%5C+are%5C+isolated%5C+at%5C+the%5C+first%5C+time.%5C+Moreover%2C%5C+it%5C+is%5C+firstly%5C+reported%5C+4%5C+new%5C+%CE%B2%5C-carboline%5C+alkaloids%5C+with%5C+a%5C+3%5C-substitution.%5C+The%5C+biosynthesis%5C+pathway%5C+of%5C+all%5C+types%5C+of%5C+alkaloids%5C+especially%5C+new%5C+compounds%5C+are%5C+proposed.%5C+At%5C+the%5C+same%5C+time%2C%5C+the%5C+bioassay%5C+of%5C+part%5C+of%5C+alkaloids%5C+were%5C+performed.In%5C+this%5C+paper%2C%5C+two%5C+unusual%5C+%E2%80%9Cchemical%5C+phenomenons%E2%80%9D%5C+were%5C+stated%5C+and%5C+explored.Moreover%2C%5C+two%5C+novel%5C+daphnane%5C+diterpenes%5C+with%5C+unusual%5C+4%2C6%5C-epoxide%5C+and%5C+six%5C+totaxins%5C+and%5C+sterols%5C+were%5C+isolated%5C+from%5C+trigonostemon%5C+thyrsoideus.%5C+And%5C+then%2C%5C+one%5C+phenylpropanoid%5C+and%5C+five%5C+sterols%5C+were%5C+found%5C+from%5C+Buddleja%5C+Alternifolia.The%5C+review%5C+outline%5C+indole%5C+alkaloids%5C+and%5C+their%5C+anti%5C-tumor%5C+activities."},{"jsname":"Key Laboratory of Ethnomedicine (Minzu University of China) of Ministry of Education of China[KLEM-ZZ201806]","jscount":"1","jsurl":"/simple-search?field1=all&field=dc.type_filter&advanced=false&query1=HYBRIDS&&fq=dc.project.title_filter%3AKey%5C+Laboratory%5C+of%5C+Ethnomedicine%5C+%5C%28Minzu%5C+University%5C+of%5C+China%5C%29%5C+of%5C+Ministry%5C+of%5C+Education%5C+of%5C+China%5C%5BKLEM%5C-ZZ201806%5C%5D"},{"jsname":"Kunming Institute of Botany, Chinese Academy of Sciences","jscount":"1","jsurl":"/simple-search?field1=all&field=dc.type_filter&advanced=false&query1=HYBRIDS&&fq=dc.project.title_filter%3AKunming%5C+Institute%5C+of%5C+Botany%2C%5C+Chinese%5C+Academy%5C+of%5C+Sciences"},{"jsname":"Kunming Institute of Botany[KIB2017003]","jscount":"1","jsurl":"/simple-search?field1=all&field=dc.type_filter&advanced=false&query1=HYBRIDS&&fq=dc.project.title_filter%3AKunming%5C+Institute%5C+of%5C+Botany%5C%5BKIB2017003%5C%5D"},{"jsname":"Major Program of CAS[KSZD-EW-Z-004-01]","jscount":"1","jsurl":"/simple-search?field1=all&field=dc.type_filter&advanced=false&query1=HYBRIDS&&fq=dc.project.title_filter%3AMajor%5C+Program%5C+of%5C+CAS%5C%5BKSZD%5C-EW%5C-Z%5C-004%5C-01%5C%5D"},{"jsname":"Ministry of Education of China","jscount":"1","jsurl":"/simple-search?field1=all&field=dc.type_filter&advanced=false&query1=HYBRIDS&&fq=dc.project.title_filter%3AMinistry%5C+of%5C+Education%5C+of%5C+China"},{"jsname":"Ministry of Science and Technology, China[2013FY112600]","jscount":"1","jsurl":"/simple-search?field1=all&field=dc.type_filter&advanced=false&query1=HYBRIDS&&fq=dc.project.title_filter%3AMinistry%5C+of%5C+Science%5C+and%5C+Technology%2C%5C+China%5C%5B2013FY112600%5C%5D"},{"jsname":"Minzu University of China[2015MDTD16C]","jscount":"1","jsurl":"/simple-search?field1=all&field=dc.type_filter&advanced=false&query1=HYBRIDS&&fq=dc.project.title_filter%3AMinzu%5C+University%5C+of%5C+China%5C%5B2015MDTD16C%5C%5D"},{"jsname":"Minzu University of China[YLDXXK201819]","jscount":"1","jsurl":"/simple-search?field1=all&field=dc.type_filter&advanced=false&query1=HYBRIDS&&fq=dc.project.title_filter%3AMinzu%5C+University%5C+of%5C+China%5C%5BYLDXXK201819%5C%5D"},{"jsname":"lastIndexed","jscount":"2024-05-24"}],"资助项目","dc.project.title_filter")'>
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