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中国科学院昆明植物研究所知识管理系统
Knowledge Management System of Kunming Institute of Botany,CAS
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6 could use lots of photosynthates, but contributed little to the accumulation of biomass. 4. Photosynthetic rate of P. armeniacum decreased a little at the noon, and the highest photosynthetic rate was observed at 10:00h in the greenhouse. The variation of photosynthetic rate was in the same trend as stomatal conductance. Higher relative humidity seemed to be the key for higher photosynthetic rate in P. armeniacum. 5. The photosynthetic capacity of C. flavum was statistically larger than that of P. armeniacum. The lower leaf photosynthetic capacity of P. armeniacum was related to its lower leaf nitrogen concentration,leaf phosphorus concentration and enzyme activities. Meanwhile, the extremely lower stomatal conductance and internal mesophyll conductance might greatly limit the photosynthetic capacity of P. armeniacum. The lower stomatal conductance and photosynthetic rate of Paphiopedilum might partially caused by the lack of chloroplasts in the guard cell of Paphiopedilum. Compared with C. flavum, P. armeniacum was more fond of shade environment.6. The short longevity leaf of Cypripedium had bigger photosynthetic capacity and greater potential for fast growth. But the longer LL of Paphiopedilum enhanced nutrient conservation which could compensate its lower photosynthetic capacity. The short longevity leaf of Cypripedium usually had higher photosynthetic rate per unit leaf mass and dark respiration rate, and photosynthetic capacity decreased fast with leaf age. However, for Paphiopedilum, the situation was the opposite. 7. Compared with Cypripedium, Paphiopedilum had higher water use efficiency and lower photosynthetic nitrogen use efficiency. 8. The leaf of Paphiopedilum had higher leaf construction cost and longer repayment time than that of Cypripedium. The leaf structures and physiological functions of Paphiopedilum and Cypripedium reflected the adaptation to their habitats. The leaf morphological and physiological evolution of Paphiopedilum was related to water and resource-conserving traits in the karst habitat. The leaf traits of Cypripedium were the adaptation to the environment rich in water and nutrients but easy to change with seasons.Our results provided evidence of divergent evolution of congeneric orchids under natural 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and Cypripedium，known as slipper orchids in horticulture, belong to the subfamily Cypripedioideae of the Orchidaceae. Although they are closely related phylogenetically, there are significant differences in leaf traits and geographical distributions between two genera. This dissertation includes the following sections: (1) the leaf functional traits were compared in six species of the two genera; (2) the physiological responses of P. armeniacum to different water regimes, light regimes and low temperature; (3) the leaf phenotypic plastics of C. flavum in response to the different light condition and the photosynthetic characteristics of three Cypripedium species during sexual reproduction. The aims are to understand the convergent and divergent evolution between the two genera in leaf traits and their adaptive significances, and the leaf plastic responses to different levels of resources. Such information could provide scientific basis for conservation and domestication of Paphiopedilum and Cypripedium. The results are given below:1. Compared with Paphiopedilum, Cypripedium showed significantly higher photosynthetic rate (Pmax), leaf nitrogen content (Na), photosynthetic nitrogen utilization (PNUE), the fractions of leaf nitrogen partitioning in carboxylation (PC) and bioenergetics (PB), specific leaf area (SLA), ratio of leaf chlorophyll a and b (Chla/b), but significantly lower leaf construction cost (CC) and the ratio of leaf carbon content to leaf nitrogen (C/N). These leaf traits of Cypripedium are considered as the adaptation to short growing period and rich soil nutrients in the alpine habitats. Conversely, the long life span, low Pmax and mesophyll conductance (gm) but high SLA, CC and C/N in Paphiopedilum indicated that the adaptation to low-light, limited-nutrient habitat in the limestone area. As a sympatric species of Paphiopedilum, C. lentiginosum not only kept phylogenetically leaf traits of Cypripedium, suchas stomatal conductance (gs), Pmax, PNUE and dormant in winter, but also possessed many leaf traits which is similar to that in Paphiopedilum, such as relative stomatal limitations (RSL), gm, the ratio of leaf chlorophyll a and b (Chl a/b), fraction of leaf nitrogen allocated to light-harvesting components (PL). These results indicated the convergent and divergent evolution of Paphiopedilum and Cypripedium in leaf traits.2. Paphiopedilum. armeniacum exhibited a high plasticity of leaf photosynthetic function in response to different light regimes, but the responses changes with the time. Due to grow under low light habitat, P. armeniacum grown under 50% shade (HL) had the significantly lowest Pmax than the plants grown under 75% shade (ML) and 95% shade (LL) after six months. However, after twelve months, the Pmax of the plants grown under HL increased significantly and then became the highest one among three levels of light. It is also found that leaf dry mass per unit area (LMA), leaf stomatal conductance (gS), internal mesophyll conductance (gm), the fraction of leaf nitrogen partitioning in photosynthetic carboxylation (PC), bioeneretics (PB) were greatly influenced by irradiance. The plants grown under HL increased gS, gm, PC, PB to increase Pmax. In addition, the plants grown under HL had the highest ratio of total chlorophyll content to total Carotenoid content (Car/Chl) while the plants grown under LL had the lowest ratio of leaf chlorophyll a and b (Chl a/b). As a result, plasticity of leaf photosynthetic physiology of P. armeniacum in response to different light regimes depended largely on leaf nitrogen partitioning and leaf structure. As for the numbers of flowering and fruiting, ML was the best light level.3. The responses of P. armeniacum to different water regimes were not significantly different. But the Pmax and the maximum photochemical efficiency of PSⅡ (Fv/Fm) decreased with the increased frequency of watering. The reasons were that the plants have high respiration rate (Rd) and make more use of light energy to oxidation cycle. The plants watered every eight days (MW) and every twenty days (LW) had higher Pmax than the plant watered every four days (HW) mainly because of the higher PC and PB. Besides, the leaves of P. armeniacum had excellent property for holding water also contributed to the high photosynthetic capacity.4. Paphiopedilum. armeniacum was very sensitive to the low temperature. The plants significantly decreased photosynthetic capacity after grown under 4℃ for three days and the photosynthetic machinery was destroyed after fifteen days. The photosynthetic capacity of P. armeniacum exhibited no change at 10℃ and 15℃.5. Cypripedium flavum of four habitats (DB, XRD, XZD and TSQ) with different light intensity exhibited different photosynthetic characteristics after transplanted to the same environment in Kunming. Among the habitats, the light intensity of DB was the highest while XRD was the lowest. The light intensity of XZD and TSQ were not significantly difference. Among all the plants in Kunming, the plants of DB had the significantly highest Pmax but the plants of XRD had the lowest Pmax. The light saturation point (LSP) and photosynthetic nitrogen use efficiency (PNUE) agreed well with the light intensity of four habitats and contributed to the high Pmax of DB. The LMA, Chl and leaf nitrogen content were not different among all the plants. C. flavum exhibited sensitively response to the change of light in leaf construction while kept the plasticity of leaf photosynthetic characteristics which developed from its own habitat.6. The photosynthetic capacity of C. tibeticum and C. flavum were significantly increased at the flowering stage. For these two species, the significantly increased Amax were closely related to the maximum carboxylation rate by ribulose-1, 5-bisphosphate carboxylase/oxygenase (Vcmax), photon saturated rate of electron transport (Jmax), the rate of triose phosphate utilization (TPU) and actual quantum efficiency of the photosystem II photochemistry (ΦPSII) respectively. However, flowering almost did not affect the photosynthetic capacity of C. guttatum. C. guttatum had the smallest plant size, the leaf area, the volume of labellum and the volume of fruit, but the biggest fruit volume per leaf area among three species. These results indicated that for C. flavum and C. tibeticum there were a physiological mechanism in photosynthesis to compensate the cost of flowering as well as increased resource acquisitions, which would be beneficial to the survival or future flowering of the plant. C. gutattum could keep a steady photosynthetic capacity during life history. This kind of pattern could decrease the effect of the reproductive costs as much as possible. In contrast to C. flavum and C. tibeticum, C. gutattum possessed a more economical and effective reproductive pattern which maybe related to its wider distribution.In conclusion, Paphiopedilum and Cypripedium have significantly different leaf traits which agree well with their habitats and there is a divergent and convergent evolution between the two genera. P. armeniacum is much tolerant and responsive to varying water and light availability but very sensitivity to the low temperature. Confronting the suddenly change of light environment, C. flavum can respond sensitively to the change of light in leaf construction but the plasticity of leaf photosynthetic characteristics which developed from its own habitat can hold for the next growing season. In contrast to C. flavum and C. tibeticum, C. gutattum possesses a more economical and effective reproductive pattern which maybe related to its wider distribution. The study of the relationship between the two genera, the response and tolerance to the environmental factors of the two genera are important for understanding the adaptation and evolution of the 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secondary metabolites are a class of small molecular compounds which are not necessary for plant growth and development in nature. They have many types and different functions. The current studies about these compounds were often focused on the chemistry, pharmacology and drug development. Research about their biological effects is few. Exploration the biological effects of plant secondary metabolites is important to study their biological function and application. Scutellarin is an active pharmaceutical ingredient extracted from Erigeron breviscapus(vant) Hand Mass. It is an important plant secondary metabolite, belonging to flavonoid. Previous studies found that some flavonoids such as quercetin, naringenin were auxin transport inhibitors in plants and they were related to plant growth and development closely. As an important flavonoid, scutellarin also plays an important role in plant growth and development is unknown. In this paper, scutellarin was selected as research object. Through a series of plant physiological, biochemical and molecular techniques to explore the biological effects of scutellarin on Arabidopsis thaliana, we get the results as follows: Scutellarin had a hormone-like effect on Arabidopsis thaliana. It promoted root elongation at low concentration obviously; however the effect disappeared at high concentration. We made a further study about it and found that scutellarin had antagonism with methyl jasmonate and 2,4-dichlorophenoxy acetic acid (2,4-D) in root elongation. It also had an influence on nitrogen metabolism. Microarray results showed that the biological effects of scutellarin had a complex relationship with plant hormone and nitrogen metabolism. These were consistent with our experimental phenomenas. All these manifested that scutellarin played an important role in plant growth and development as a similar plant hormone. We tried the experiment using some other flavonoids. It was found that not all the flavonoids had the same obvious effects on root elongation like scutellarin; the biological effects of them were closely related to their chemical structures. In addition, the other two aspects of research were also carried out in the paper. One was exploring the role of phospholipase Dδ (PLDδ) under ultraviolet radiation in Arabidopsis. Our evidences suggested knockout PLDδ intensified membrane damage induced by UV radiation. The other one was lipid data collection and calculation of acyl chain lengths of lipid molecules in Arabidopsis under senescence induced by various stresses. We showed that the acyl chains of phosphoserine (PS, a head-group class of membrane glycerolipids with very long chains of fatty acids.) lengthened with the development and senescence in Arabidopsis. In contrast, the acyl chain lengths of other major head-group classes of membrane glycerolipids subtly fluctuated.","jscount":"1","jsurl":"/simple-search?field1=all&field=dc.date.issued.year&advanced=false&fq=dc.type_filter%3A%E5%AD%A6%E4%BD%8D%E8%AE%BA%E6%96%87&query1=Physiological%2BMechanism&&fq=dc.project.title_filter%3APlant%5C+secondary%5C+metabolites%5C+are%5C+a%5C+class%5C+of%5C+small%5C+molecular%5C+compounds%5C+which%5C+are%5C+not%5C+necessary%5C+for%5C+plant%5C+growth%5C+and%5C+development%5C+in%5C+nature.%5C+They%5C+have%5C+many%5C+types%5C+and%5C+different%5C+functions.%5C+The%5C+current%5C+studies%5C+about%5C+these%5C+compounds%5C+were%5C+often%5C+focused%5C+on%5C+the%5C+chemistry%2C%5C+pharmacology%5C+and%5C+drug%5C+development.%5C+Research%5C+about%5C+their%5C+biological%5C+effects%5C+is%5C+few.%5C+Exploration%5C+the%5C+biological%5C+effects%5C+of%5C+plant%5C+secondary%5C+metabolites%5C+is%5C+important%5C+to%5C+study%5C+their%5C+biological%5C+function%5C+and%5C+application.%5C+Scutellarin%5C+is%5C+an%5C+active%5C+pharmaceutical%5C+ingredient%5C+extracted%5C+from%5C+Erigeron%5C+breviscapus%5C%28vant%5C%29%5C+Hand%5C+Mass.%5C+It%5C+is%5C+an%5C+important%5C+plant%5C+secondary%5C+metabolite%2C%5C+belonging%5C+to%5C+flavonoid.%5C+Previous%5C+studies%5C+found%5C+that%5C+some%5C+flavonoids%5C+such%5C+as%5C+quercetin%2C%5C+naringenin%5C+were%5C+auxin%5C+transport%5C+inhibitors%5C+in%5C+plants%5C+and%5C+they%5C+were%5C+related%5C+to%5C+plant%5C+growth%5C+and%5C+development%5C+closely.%5C+As%5C+an%5C+important%5C+flavonoid%2C%5C+scutellarin%5C+also%5C+plays%5C+an%5C+important%5C+role%5C+in%5C+plant%5C+growth%5C+and%5C+development%5C+is%5C+unknown.%5C+In%5C+this%5C+paper%2C%5C+scutellarin%5C+was%5C+selected%5C+as%5C+research%5C+object.%5C+Through%5C+a%5C+series%5C+of%5C+plant%5C+physiological%2C%5C+biochemical%5C+and%5C+molecular%5C+techniques%5C+to%5C+explore%5C+the%5C+biological%5C+effects%5C+of%5C+scutellarin%5C+on%5C+Arabidopsis%5C+thaliana%2C%5C+we%5C+get%5C+the%5C+results%5C+as%5C+follows%5C%3A%5C+Scutellarin%5C+had%5C+a%5C+hormone%5C-like%5C+effect%5C+on%5C+Arabidopsis%5C+thaliana.%5C+It%5C+promoted%5C+root%5C+elongation%5C+at%5C+low%5C+concentration%5C+obviously%5C%3B%5C+however%5C+the%5C+effect%5C+disappeared%5C+at%5C+high%5C+concentration.%5C+We%5C+made%5C+a%5C+further%5C+study%5C+about%5C+it%5C+and%5C+found%5C+that%5C+scutellarin%5C+had%5C+antagonism%5C+with%5C+methyl%5C+jasmonate%5C+and%5C+2%2C4%5C-dichlorophenoxy%5C+acetic%5C+acid%5C+%5C%282%2C4%5C-D%5C%29%5C+in%5C+root%5C+elongation.%5C+It%5C+also%5C+had%5C+an%5C+influence%5C+on%5C+nitrogen%5C+metabolism.%5C+Microarray%5C+results%5C+showed%5C+that%5C+the%5C+biological%5C+effects%5C+of%5C+scutellarin%5C+had%5C+a%5C+complex%5C+relationship%5C+with%5C+plant%5C+hormone%5C+and%5C+nitrogen%5C+metabolism.%5C+These%5C+were%5C+consistent%5C+with%5C+our%5C+experimental%5C+phenomenas.%5C+All%5C+these%5C+manifested%5C+that%5C+scutellarin%5C+played%5C+an%5C+important%5C+role%5C+in%5C+plant%5C+growth%5C+and%5C+development%5C+as%5C+a%5C+similar%5C+plant%5C+hormone.%5C+We%5C+tried%5C+the%5C+experiment%5C+using%5C+some%5C+other%5C+flavonoids.%5C+It%5C+was%5C+found%5C+that%5C+not%5C+all%5C+the%5C+flavonoids%5C+had%5C+the%5C+same%5C+obvious%5C+effects%5C+on%5C+root%5C+elongation%5C+like%5C+scutellarin%5C%3B%5C+the%5C+biological%5C+effects%5C+of%5C+them%5C+were%5C+closely%5C+related%5C+to%5C+their%5C+chemical%5C+structures.%5C+In%5C+addition%2C%5C+the%5C+other%5C+two%5C+aspects%5C+of%5C+research%5C+were%5C+also%5C+carried%5C+out%5C+in%5C+the%5C+paper.%5C+One%5C+was%5C+exploring%5C+the%5C+role%5C+of%5C+phospholipase%5C+D%CE%B4%5C+%5C%28PLD%CE%B4%5C%29%5C+under%5C+ultraviolet%5C+radiation%5C+in%5C+Arabidopsis.%5C+Our%5C+evidences%5C+suggested%5C+knockout%5C+PLD%CE%B4%5C+intensified%5C+membrane%5C+damage%5C+induced%5C+by%5C+UV%5C+radiation.%5C+The%5C+other%5C+one%5C+was%5C+lipid%5C+data%5C+collection%5C+and%5C+calculation%5C+of%5C+acyl%5C+chain%5C+lengths%5C+of%5C+lipid%5C+molecules%5C+in%5C+Arabidopsis%5C+under%5C+senescence%5C+induced%5C+by%5C+various%5C+stresses.%5C+We%5C+showed%5C+that%5C+the%5C+acyl%5C+chains%5C+of%5C+phosphoserine%5C+%5C%28PS%2C%5C+a%5C+head%5C-group%5C+class%5C+of%5C+membrane%5C+glycerolipids%5C+with%5C+very%5C+long%5C+chains%5C+of%5C+fatty%5C+acids.%5C%29%5C+lengthened%5C+with%5C+the%5C+development%5C+and%5C+senescence%5C+in%5C+Arabidopsis.%5C+In%5C+contrast%2C%5C+the%5C+acyl%5C+chain%5C+lengths%5C+of%5C+other%5C+major%5C+head%5C-group%5C+classes%5C+of%5C+membrane%5C+glycerolipids%5C+subtly%5C+fluctuated."},{"jsname":"Seeds contain the entire genetic information of plants, which enable to develop the technology of seed preservation for the conservation of plant diversity. The mechanism of cell death in seeds, which are induced by ageing of orthodox seeds and desiccation of recalcitrant and intermediate seeds are two important areas in seed biology but poorly understood.Those two areas were investigated in this dissertation, and Pisum sativum, Camellia sinensis var. assamica, Castanea mollissima were chosen to represent orthodox, intermediate and recalcitrant seeds respectively as studied models. Biophysical, biochemical and molecular methods were employed to investigate the cell death mechanism during seeds ageing and desiccation.First of all, Ageing induced porgrammed cell death in seeds.The morphology change, nucleic acid integrity, and gene expression during ageing of Pisum sativum seeds were investigated.The data above confirmed that PCD is an underlying mechanism of seed mortality. Moreover, glutathione played an important function during seeds ageing.An increase in glutathione redox potential (EGSSG/2GSH) while seed lost viability was discovered during ageing of P. sativum seeds. Seed viability decreased 50% when EGSSG/2GSH attained values of -180 to -160 mV, which proved that EGSSG/2GSH was a marker for seeds viability. Secondely,Reactive oxygen species plays different roles in the seed germination after desiccation. After different desiccation treatments on the tea (C. sinensis var. assamica) seeds, the relationship between ROS dose and the viability of seeds after desication treatment was investigated. The results demonstrated that the presence of ROS could released the damage of desiccation on the tea seeds. Thridly,the composition of membrane lipid species and the change during desiccation in seeds with different storage behavior. Cell membrane is very sensitive to environment, and adjust the composition of lipid molecules is one of the main methord for cell to response the environmental changes. There is a rule that phosphatidic acid (PA), with special structure, is very sensitive to temperature and water stress, and the increase of PA content indicates the damage of the cell membrane. ESI/MS-MS technology was used to analysis and compare the composition and changes membrane lipid species during desiccation treatments of Pisum sativum, Camellia sinensis var. assamica, Castanea mollissima seeds which represented orthodox, intermediate and recalcitrant seeds respectively. The results showed that the responses of PA to dehydration in embryonic axis of orhtordox and recalcitrant seeds were different. The percentage increasement of PA in orthodox seed during desiccation was not incredible and the level of PA decreased and achieved the same level as controled, while PA level increased dramatically without showing any decrease trend in recalcitrant seed, which indicated that the damage to membrane of recalcitrant seeds was inevitable. Those results indicted that the dehydration might induced reversible memebrane damage in the orthodox seed, which may result in no affect on the seed viability, while it might induced irreversible membrane damage to the recalcitrant seed, which inducned the dramatically reduce of viabiligy and cell death in the end. It was the first time to investigate and analysis systematically the composition and changes of membrane lipids of different storage behavior seeds during desiccation. It was first time to report the relationship between PA and the seeds storeage and the change of PA level in seeds could be used as the marker to diagnose.the property of seeds for desiccation sensitivity or desiccation tolerance","jscount":"1","jsurl":"/simple-search?field1=all&field=dc.date.issued.year&advanced=false&fq=dc.type_filter%3A%E5%AD%A6%E4%BD%8D%E8%AE%BA%E6%96%87&query1=Physiological%2BMechanism&&fq=dc.project.title_filter%3ASeeds%5C+contain%5C+the%5C+entire%5C+genetic%5C+information%5C+of%5C+plants%2C%5C+which%5C+enable%5C+to%5C+develop%5C+the%5C+technology%5C+of%5C+seed%5C+preservation%5C+for%5C+the%5C+conservation%5C+of%5C+plant%5C+diversity.%5C+The%5C+mechanism%5C+of%5C+cell%5C+death%5C+in%5C+seeds%2C%5C+which%5C+are%5C+induced%5C+by%5C+ageing%5C+of%5C+orthodox%5C+seeds%5C+and%5C+desiccation%5C+of%5C+recalcitrant%5C+and%5C+intermediate%5C+seeds%5C+are%5C+two%5C+important%5C+areas%5C+in%5C+seed%5C+biology%5C+but%5C+poorly%5C+understood.Those%5C+two%5C+areas%5C+were%5C+investigated%5C+in%5C+this%5C+dissertation%2C%5C+and%5C+Pisum%5C+sativum%2C%5C+Camellia%5C+sinensis%5C+var.%5C+assamica%2C%5C+Castanea%5C+mollissima%5C+were%5C+chosen%5C+to%5C+represent%5C+orthodox%2C%5C+intermediate%5C+and%5C+recalcitrant%5C+seeds%5C+respectively%5C+as%5C+studied%5C+models.%5C+Biophysical%2C%5C+biochemical%5C+and%5C+molecular%5C+methods%5C+were%5C+employed%5C+to%5C+investigate%5C+the%5C+cell%5C+death%5C+mechanism%5C+during%5C+seeds%5C+ageing%5C+and%5C+desiccation.First%5C+of%5C+all%2C%5C+Ageing%5C+induced%5C+porgrammed%5C+cell%5C+death%5C+in%5C+seeds.The%5C+morphology%5C+change%2C%5C+nucleic%5C+acid%5C+integrity%2C%5C+and%5C+gene%5C+expression%5C+during%5C+ageing%5C+of%5C+Pisum%5C+sativum%5C+seeds%5C+were%5C+investigated.The%5C+data%5C+above%5C+confirmed%5C+that%5C+PCD%5C+is%5C+an%5C+underlying%5C+mechanism%5C+of%5C+seed%5C+mortality.%5C+Moreover%2C%5C+glutathione%5C+played%5C+an%5C+important%5C+function%5C+during%5C+seeds%5C+ageing.An%5C+increase%5C+in%5C+glutathione%5C+redox%5C+potential%5C+%5C%28EGSSG%5C%2F2GSH%5C%29%5C+while%5C+seed%5C+lost%5C+viability%5C+was%5C+discovered%5C+during%5C+ageing%5C+of%5C+P.%5C+sativum%5C+seeds.%5C+Seed%5C+viability%5C+decreased%5C+50%25%5C+when%5C+EGSSG%5C%2F2GSH%5C+attained%5C+values%5C+of%5C+%5C-180%5C+to%5C+%5C-160%5C+mV%2C%5C+which%5C+proved%5C+that%5C+EGSSG%5C%2F2GSH%5C+was%5C+a%5C+marker%5C+for%5C+seeds%5C+viability.%5C+Secondely%2CReactive%5C+oxygen%5C+species%5C+plays%5C+different%5C+roles%5C+in%5C+the%5C+seed%5C+germination%5C+after%5C+desiccation.%5C+After%5C+different%5C+desiccation%5C+treatments%5C+on%5C+the%5C+tea%5C+%5C%28C.%5C+sinensis%5C+var.%5C+assamica%5C%29%5C+seeds%2C%5C+the%5C+relationship%5C+between%5C+ROS%5C+dose%5C+and%5C+the%5C+viability%5C+of%5C+seeds%5C+after%5C+desication%5C+treatment%5C+was%5C+investigated.%5C+The%5C+results%5C+demonstrated%5C+that%5C+the%5C+presence%5C+of%5C+ROS%5C+could%5C+released%5C+the%5C+damage%5C+of%5C+desiccation%5C+on%5C+the%5C+tea%5C+seeds.%5C+Thridly%2Cthe%5C+composition%5C+of%5C+membrane%5C+lipid%5C+species%5C+and%5C+the%5C+change%5C+during%5C+desiccation%5C+in%5C+seeds%5C+with%5C+different%5C+storage%5C+behavior.%5C+Cell%5C+membrane%5C+is%5C+very%5C+sensitive%5C+to%5C+environment%2C%5C+and%5C+adjust%5C+the%5C+composition%5C+of%5C+lipid%5C+molecules%5C+is%5C+one%5C+of%5C+the%5C+main%5C+methord%5C+for%5C+cell%5C+to%5C+response%5C+the%5C+environmental%5C+changes.%5C+There%5C+is%5C+a%5C+rule%5C+that%5C+phosphatidic%5C+acid%5C+%5C%28PA%5C%29%2C%5C+with%5C+special%5C+structure%2C%5C+is%5C+very%5C+sensitive%5C+to%5C+temperature%5C+and%5C+water%5C+stress%2C%5C+and%5C+the%5C+increase%5C+of%5C+PA%5C+content%5C+indicates%5C+the%5C+damage%5C+of%5C+the%5C+cell%5C+membrane.%5C+ESI%5C%2FMS%5C-MS%5C+technology%5C+was%5C+used%5C+to%5C+analysis%5C+and%5C+compare%5C+the%5C+composition%5C+and%5C+changes%C2%A0membrane%5C+lipid%5C+species%5C+during%5C+desiccation%5C+treatments%5C+of%5C+Pisum%5C+sativum%2C%5C+Camellia%5C+sinensis%5C+var.%5C+assamica%2C%5C+Castanea%5C+mollissima%5C+seeds%5C+which%5C+represented%5C+orthodox%2C%5C+intermediate%5C+and%5C+recalcitrant%5C+seeds%5C+respectively.%5C+The%5C+results%5C+showed%5C+that%5C+the%5C+responses%5C+of%5C+PA%5C+to%5C+dehydration%5C+in%5C+embryonic%5C+axis%5C+of%5C+orhtordox%5C+and%5C+recalcitrant%5C+seeds%5C+were%5C+different.%5C+The%5C+percentage%5C+increasement%5C+of%5C+PA%5C+in%5C+orthodox%5C+seed%5C+during%5C+desiccation%5C+was%5C+not%5C+incredible%5C+and%5C+the%5C+level%5C+of%5C+PA%5C+decreased%5C+and%5C+achieved%5C+the%5C+same%5C+level%5C+as%5C+controled%2C%5C+while%5C+PA%5C+level%5C+increased%5C+dramatically%5C+without%5C+showing%5C+any%5C+decrease%5C+trend%5C+in%5C+recalcitrant%5C+seed%2C%5C+which%5C+indicated%5C+that%5C+the%5C+damage%5C+to%5C+membrane%5C+of%5C+recalcitrant%5C+seeds%5C+was%5C+inevitable.%C2%A0Those%5C+results%5C+indicted%5C+that%5C+the%5C+dehydration%5C+might%5C+induced%5C+reversible%5C+memebrane%5C+damage%5C+in%5C+the%5C+orthodox%5C+seed%2C%5C+which%5C+may%5C+result%5C+in%5C+no%5C+affect%5C+on%5C+the%5C+seed%5C+viability%2C%5C+while%5C+it%5C+might%5C+induced%5C+irreversible%5C+membrane%5C+damage%5C+to%5C+the%5C+recalcitrant%5C+seed%2C%5C+which%5C+inducned%5C+the%5C+dramatically%5C+re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a rare genus endemic to China, has two species S. grevilleoides and S. albofusca. To explore systematic position and endangerment mechanisms of Sinopteris, its sporophyte morphological characteristics, gametophyte development, cytology, molecular phologenetics, and biogeography were investigated based on the extensive investigation of its distribution, existing circumstances and ecological adaptation. The main progresses are briefly summarized as follows:1. Resource investigation, S. grevilleoides has a scattered distribution only in very few areas of Dayao, Binchuan, Qiaojia in Yunnan and Qingchuan in Sichuan; the elevation scope of its habitate is 1100-1800m. S. albofusca distributes in areas, 1700-2800 meters above sealevel in Hebei, Beijing, Yunnan, Sichuan, Guangxi, Hunan and Guizhou. Our investigation showed that the resources of the two species were decreasing and it was very difficult to find them in wild, even in distribution areas on record. 2. Sporophyte morphological characteristics, The plants of Sinopteris are evergreen small xerophytes. Fronds are texture coriaceous, pentagon, upper surface smooth, under surface whitish-farinose; veins pinnate branching, not seen above, but very prominently raised beneath. Sori are marginal, terminal, consisting of one or two large, globose, subsessile sporangium, provided with a very broad annulus. Indusia deeply cut into triangular, toothed lobes. The form of frond epidermis of S. grevilleoides and S. albofusca bring into correspondence with each other: the epidermis cells are irregular narrow strip with sinuous anticlinal walls; the stomatal apparatus exists on the lower epidermis and its types according to Dilcher’s [26] nomination are polycytic and axillocytic. Scales attaching to the base of petiole are brown, lanceolate.3. Gametophyte development, The spores of S. grevilleoides and S. albofusca were cultured in improved Knop’s agar medium and three kinds of soil substrata respectively. Spore germination and gametophyte development were observed. The impact of four culture substrata on the gametophyte development and sexual reproduction of S. grevilleoides was compared. The results are as follows:(1) The mature spores of Sinopteris were black-brown, isospory, regular tetrahedron, trilete, blunt triangle in polar view, scoop in equatorial view. Spore germination was of Vittaria-type and gametophyte exhibited Adiantum-type development. The adult prothalli were symmetric cordate. Antheridium protruding from the surface of prothalus was nearly spherosome. Archegonium was born near the notch on the ventral face of the cordate prothalus and those near the notch matured late. Mature archegonium was tall and slender, whose neck was composed of four lines cells and three to five layerscells each line. The four cells at the top separated when the archegonium matured. (2) The gametophyte and infant sporophyte morphological development of S. grevilleoides represented very different features on different culture substrata. Mature cordate prothalli on humus soil did not bear archegonias, and thus the process of sexual reproduction ofS. grevilleoides could not be finished. The substratum composed of humus soil and original soil in ratio of 1:1 was the most suitable substratum for gametophyte development and sexual reproduction of this species. Infant sporophytes needed extra nutrient solution to sustain its growth on improved Knop''s agar medium.(3) The main contaminants were bacteria, fungi and algae during sterile cultures and algae, moss and other higher plants during soil cultures.4. Cytology, Chromosome numbers of S. grevilleoides and S. albofusca were investigated firstly. The result showed that the chromosome numbers of the two species both were 2n=60 and the basic choromosome number was x=30. 5. Molecular phologenetics, The phylogeny of 26 species was estimated from combined analyses of four cpDNA sequence data sets (rps4, rps4-trnS, atpB, atpB-rbcL and trnL-F). Most parsimonious (MP) and Bayesian analysis both shows the genus Sinopteris is monophyletic with strong support and it is nearly related to A. subargentea from Ser. Argentea of Aleuritopteris. The MPsystematic tree also shows: both Leptolepidium and Cheilosoria are not monophyletic; Cheilosoria is nearly related to Ser. Argentea of Aleuritopteris and Leptolepidium is nearly related to Ser. Farinosae of Aleuritopteris.6. Population and community structure, The two species of Sinopteris mainly grow in the crevices of cliffs and the numbers of individuals within populations are small. In the community, they are less competitive and under dog. Its geographical distribution requires strict elevation, humidity and illumination. S. grevilleoides often grows in the brushwood or grass. Thickets of S. albofusca usually grows in the brushwood or coniferous forest. 7. Soil property,S. grevilleoides grows in purplish soil in wild; the soil is very thin and it isdifficult to store water. S. albofusca grows in sticky and infertile red soil. Analysis of the soil chemical property shows: the two original soils both are acid soil with high calcium and available B; their total Pand K is low.8. Endangerment factors and conservation measures, Endangerment factors: (1) Their distribution areas are so narrow that they are difficult to cope with environmental changes. (2)Their own physiological and ecological characteristics: spore germination was exigent over humidity, illumination and soil; their ecological circumstances cause their rhizomes to have no too much room to extend. Based on the previous two, reproduction by spores and rhizomes for the genus Sinopteris is limited. (3)To some extent, human-induced habitat loss, accompanying habit fragmentation, and natural disaster such as drought and fire accelerated the endangerment process. Conservation measures: (1) Have a clear and definite acquaintance to recent population size, distribution and growth dynamics by strengthening field investigation. (2) According to the specific circumstances, introduce in-situ and ex-situ conservation. (3)Rejuvenate and expand Sinopteris by adopting division propagation and artificial reproduction by spores. (4) Exploit species of the genus as greening and floral 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dissertation is composed of eight chapters. In chapters 1-7, the chemical and bioactive constituents of seven higher fungi, Boreostereum vibrans, Hexagonia speciosa, Panaeolus leucophanes, Gomphus purpuraceus, Sparassis crispa, Craterellus aureus and Hydnum repandum have been investigated. In chapter 8, a review about the chemical and bioactivity of N-containing compounds of macromycetes between 2004 and 2009 was given.100 compounds, including 36 new ones, were isolated from the above mentioned seven species. Their structures were established on the basis of extensive spectroscopic methods in combination with single-crystal X-ray diffraction analysis. The type of the isolated compounds includes sesquiterpenoids, ergostols, isocoumarins, vibralactone derivertives, as well as oxygenated cyclohexanoids.Fourteen compounds were isolated from the culture broth of basidiomycete Boreostereum vibrans (Berk & M.A. Curtis Davydkina & Bondartseva (Aphyllophorales)). Among them, seven new ones were the vibralactone derivatives. 1,5-Secovibralactone and vibralactone C exhibited moderate inhibitory effect against human 11β-HSD1 but did not exhibit inhibition on 11β-HSD2. Vibralactone B, vibralactone D and acetylated vibralactone showed inhibitory activities against both 11β-HSD1 and 11β-HSD2. Twenty new oxygenated cyclohexanoids and a new acetylated aporpinone compounds, together with seven known compounds were isolated from the broth cultures of the basidiomycete Hexagonia speciosa. Their structures were elucidated on the basis of extensive spectroscopic analysis, while the structure of 8 was confirmed by single crystal X-ray diffraction data.Phytochemical study of the culture of Panaeolus leucophanes has resulted in the isolation of thirteen compounds which of eight were isocoumarins, including four new ones. Their structures were elucidated on the basis of extensive spectroscopic analysis. A new alliacane sesquiterpene, together with 5 known compounds, have been isolated from the fruiting bodies of Gomphus purpuraceu. Their structures were elucidated by means of spectroscopic methods (1D and 2D NMR, MS, IR). Five known compounds have been isolated from the culture broth of Sparassis crispa, and five from the fruiting bodies including two new compounds. Their structures were elucidated by means of spectroscopic methods (1D and 2D NMR, MS, IR). A new hydroxyl acetylenic fatty acid, together with ten known compounds have been isolated from the fruiting bodies of Craterellus aureus, and their structures were elucidated by means of spectroscopic methods (1D and 2D NMR, MS, IR). Eleven known compounds have been isolated from the culture broth of Hydnum repandum. Their structures were elucidated by means of spectroscopic methods (1D NMR, MS).In chapter 8, the chemical, biological, and mycological literature dealing with the isolation, structure elucidation, biological activities, and synthesis of nitrogen-containing compounds from the fruiting bodies and the culture broths of macromycetes were reviewed (2004-2009).","jscount":"1","jsurl":"/simple-search?field1=all&field=dc.date.issued.year&advanced=false&fq=dc.type_filter%3A%E5%AD%A6%E4%BD%8D%E8%AE%BA%E6%96%87&query1=Physiological%2BMechanism&&fq=dc.project.title_filter%3AThis%5C+dissertation%5C+is%5C+composed%5C+of%5C+eight%5C+chapters.%5C+In%5C+chapters%5C+1%5C-7%2C%5C+the%5C+chemical%5C+and%5C+bioactive%5C+constituents%5C+of%5C+seven%5C+higher%5C+fungi%2C%5C+Boreostereum%5C+vibrans%2C%5C+Hexagonia%5C+speciosa%2C%5C+Panaeolus%5C+leucophanes%2C%5C+Gomphus%5C+purpuraceus%2C%5C+Sparassis%5C+crispa%2C%5C+Craterellus%5C+aureus%5C+and%5C+Hydnum%5C+repandum%5C+have%5C+been%5C+investigated.%5C+In%5C+chapter%5C+8%2C%5C+a%5C+review%5C+about%5C+the%5C+chemical%5C+and%5C+bioactivity%5C+of%5C+N%5C-containing%5C+compounds%5C+of%5C+macromycetes%5C+between%5C+2004%5C+and%5C+2009%5C+was%5C+given.100%5C+compounds%2C%5C+including%5C+36%5C+new%5C+ones%2C%5C+were%5C+isolated%5C+from%5C+the%5C+above%5C+mentioned%5C+seven%5C+species.%5C+Their%5C+structures%5C+were%5C+established%5C+on%5C+the%5C+basis%5C+of%5C+extensive%5C+spectroscopic%5C+methods%5C+in%5C+combination%5C+with%5C+single%5C-crystal%5C+X%5C-ray%5C+diffraction%5C+analysis.%5C+The%5C+type%5C+of%5C+the%5C+isolated%5C+compounds%5C+includes%5C+sesquiterpenoids%2C%5C+ergostols%2C%5C+isocoumarins%2C%5C+vibralactone%5C+derivertives%2C%5C+as%5C+well%5C+as%5C+oxygenated%5C+cyclohexanoids.Fourteen%5C+compounds%5C+were%5C+isolated%5C+from%5C+the%5C+culture%5C+broth%5C+of%5C+basidiomycete%5C+Boreostereum%5C+vibrans%5C+%5C%28Berk%5C+%5C%26%5C+M.A.%5C+Curtis%5C+Davydkina%5C+%5C%26%5C+Bondartseva%5C+%5C%28Aphyllophorales%5C%29%5C%29.%5C+Among%5C+them%2C%5C+seven%5C+new%5C+ones%5C+were%5C+the%5C+vibralactone%5C+derivatives.%5C+1%2C5%5C-Secovibralactone%5C+and%5C+vibralactone%5C+C%5C+exhibited%5C+moderate%5C+inhibitory%5C+effect%5C+against%5C+human%5C+11%CE%B2%5C-HSD1%5C+but%5C+did%5C+not%5C+exhibit%5C+inhibition%5C+on%5C+11%CE%B2%5C-HSD2.%5C+Vibralactone%5C+B%2C%5C+vibralactone%5C+D%5C+and%5C+acetylated%5C+vibralactone%5C+showed%5C+inhibitory%5C+activities%5C+against%5C+both%5C+11%CE%B2%5C-HSD1%5C+and%5C+11%CE%B2%5C-HSD2.%5C+Twenty%5C+new%5C+oxygenated%5C+cyclohexanoids%5C+and%5C+a%5C+new%5C+acetylated%5C+aporpinone%5C+compounds%2C%5C+together%5C+with%5C+seven%5C+known%5C+compounds%5C+were%5C+isolated%5C+from%5C+the%5C+broth%5C+cultures%5C+of%5C+the%5C+basidiomycete%5C+Hexagonia%5C+speciosa.%5C+Their%5C+structures%5C+were%5C+elucidated%5C+on%5C+the%5C+basis%5C+of%5C+extensive%5C+spectroscopic%5C+analysis%2C%5C+while%5C+the%5C+structure%5C+of%5C+8%5C+was%5C+confirmed%5C+by%5C+single%5C+crystal%5C+X%5C-ray%5C+diffraction%5C+data.Phytochemical%5C+study%5C+of%5C+the%5C+culture%5C+of%5C+Panaeolus%5C+leucophanes%5C+has%5C+resulted%5C+in%5C+the%5C+isolation%5C+of%5C+thirteen%5C+compounds%5C+which%5C+of%5C+eight%5C+were%5C+isocoumarins%2C%5C+including%5C+four%5C+new%5C+ones.%5C+Their%5C+structures%5C+were%5C+elucidated%5C+on%5C+the%5C+basis%5C+of%5C+extensive%5C+spectroscopic%5C+analysis.%5C+A%5C+new%5C+alliacane%5C+sesquiterpene%2C%5C+together%5C+with%5C+5%5C+known%5C+compounds%2C%5C+have%5C+been%5C+isolated%5C+from%5C+the%5C+fruiting%5C+bodies%5C+of%5C+Gomphus%5C+purpuraceu.%5C+Their%5C+structures%5C+were%5C+elucidated%5C+by%5C+means%5C+of%5C+spectroscopic%5C+methods%5C+%5C%281D%5C+and%5C+2D%5C+NMR%2C%5C+MS%2C%5C+IR%5C%29.%5C+Five%5C+known%5C+compounds%5C+have%5C+been%5C+isolated%5C+from%5C+the%5C+culture%5C+broth%5C+of%5C+Sparassis%5C+crispa%2C%5C+and%5C+five%5C+from%5C+the%5C+fruiting%5C+bodies%5C+including%5C+two%5C+new%5C+compounds.%5C+Their%5C+structures%5C+were%5C+elucidated%5C+by%5C+means%5C+of%5C+spectroscopic%5C+methods%5C+%5C%281D%5C+and%5C+2D%5C+NMR%2C%5C+MS%2C%5C+IR%5C%29.%5C+A%5C+new%5C+hydroxyl%5C+acetylenic%5C+fatty%5C+acid%2C%5C+together%5C+with%5C+ten%5C+known%5C+compounds%5C+have%5C+been%5C+isolated%5C+from%5C+the%5C+fruiting%5C+bodies%5C+of%5C+Craterellus%5C+aureus%2C%5C+and%5C+their%5C+structures%5C+were%5C+elucidated%5C+by%5C+means%5C+of%5C+spectroscopic%5C+methods%5C+%5C%281D%5C+and%5C+2D%5C+NMR%2C%5C+MS%2C%5C+IR%5C%29.%5C+Eleven%5C+known%5C+compounds%5C+have%5C+been%5C+isolated%5C+from%5C+the%5C+culture%5C+broth%5C+of%5C+Hydnum%5C+repandum.%5C+Their%5C+structures%5C+were%5C+elucidated%5C+by%5C+means%5C+of%5C+spectroscopic%5C+methods%5C+%5C%281D%5C+NMR%2C%5C+MS%5C%29.In%5C+chapter%5C+8%2C%5C+the%5C+chemical%2C%5C+biological%2C%5C+and%5C+mycological%5C+literature%5C+dealing%5C+with%5C+the%5C+isolation%2C%5C+structure%5C+elucidation%2C%5C+biological%5C+activities%2C%5C+and%5C+synthesis%5C+of%5C+nitrogen%5C-containing%5C+compounds%5C+from%5C+the%5C+fruiting%5C+bodies%5C+and%5C+the%5C+culture%5C+broths%5C+of%5C+macromycetes%5C+were%5C+reviewed%5C+%5C%282004%5C-2009%5C%29."},{"jsname":"Trigonobalanus doichangensis is an endangered plant. In this paper, the megasporogenesis and development of female gametophyte, seed morphological traits and seed germination, seed conservation, micropropagation and acclimatization of this species were studied. Combined with the published results of cytology, molecular genetics and other researches,the mechanisms of extinction, basic biology and technology of germplasm conservation and acclimatization of T. doichangensis were discussed. The main results are summarized as follows:1. Megasporogenesis and development of female gametophyte，Stamens exist under the stigma of T. doichangensis, and the pollen is aborted on the later development stage of pistil, therefore, the pistillate flower in function is hermaphrodite flower in morphology. The ovule is anatropous, bitegmic and crassinucellate. The primary archesporium is hypodermal and single-celled and the sporogenous cell of the nucellus functions directly as a megaspore mother cell which goes meiosis to form a linear tetrad. The chalazal megaspore of the tetrad is functional. The development of embryo sac conforms to the polygonum type. There are six ovules in the ovary of T. doichangensis, and only one develops into a seed in normal fruits. In the process of megasporogenesis and development of female gametophyte, there are several links of abortion, and 93.3% of mature embryo sacs is aborted.2. Morphological characters and germination of seeds，Most of the variation occurred among individual trees within populations in seed morphological traits (length, width and 1000-seed weight) and germination-related indices (germination percentage, germination index and vigor index). In addition, the variation in percentage of well-developed seeds among populations and among individual trees within populations is equal, each accounting for 48%. Each of seed morphological traits has significantly positive correlation with each other (p < 0.01), but they have no significant correlation with percentage of well-developed seeds and germination-related indices. In the same batch of seeds of T. doichangensis, there are light-colored and dark-colored seed coats, and development of light-colored seeds is significantly poorer than that of dark-colored seeds.The sensitivity of seeds to high temperature varys in different stages of seed imbibition. In each stage, heat acclimatization don’t increase germination percentage, germination index and fresh weight of seedlings. If the distilled water is substituted by solution of SA during seed imbibition, seed germination and germination index after heat shock are not significantly different from control, but they are significantly higher than that of other treatments. Moreover, when the seeds are treatmented with SA, the fresh weight of seedlings is significantly higher than that of control and other treatments.3. Seed conservation，Seeds of T. doichangensis belong to orthodox seeds which can tolerate certain level of dehydration. The condition of low temperature and low water content of seeds is conducive to seed conservation.Germination of fresh seeds shows significant variation among populations, howerer, germination of the seeds after storage for one year in room temperature shows no significant variation among populations.High temperature and high relative humidity damages the seeds more severely than high temperature does. In addition, low water content of seeds enable the seeds to be more tolerant to high temperature.The electrical conductivity, dehydrogenase activity and germination percentage have no significant correlation with each other.4. Micropropagation and in vitro conservation，Cotyledonary nodes are a kind of efficient explants. Low salt media are conducive to shoot propagation and root induction.The maximum multiplication rate (20-25 shoots/explant within 4 months) is achieved on quarter-strength Murashige and Skoog (1/4 MS) medium supplemented with 1 mg·L-1 6-benzyladenine (6-BA) and 0.05 mg·L-1 α-naphthaleneacetic acid (NAA).Rooting is promoted by auxins, however, IBA alone or low concentrations of NAA are preferable due to small amount of callus induced. The research has established an efficient protocol for micropropagation of T. doichangensis, and it provides technology support for in vitro conservation of special germplasm of the species.5. Acclimatization，Quercus variabilis, Cyclobalanopsis glaucoides and T. doichangensis belong to the family of Fagaceae, and the natural distribution ranges of the 3 species are decreasing in turn. The research suggests that the ranges of temperature tolerance of the 3 species are decreasing corresponding to their distribution ranges.The high and low semi-lethal temperature of one-year old T. doichangensis is 49.5℃ and -5℃ respectively. It suggests that T. doichangensis has a wide range of basic temperature tolerance. Short-term heat and cold acclimatization cannot expand the range of temperature tolerance. It can be inferred that T. doichangensis may lack induced tolerance to temperature. Under proper conditions, ABA can increase the cold tolerance, and SA can increase the heat tolerance of leaf discs of T. doichangensis.","jscount":"1","jsurl":"/simple-search?field1=all&field=dc.date.issued.year&advanced=false&fq=dc.type_filter%3A%E5%AD%A6%E4%BD%8D%E8%AE%BA%E6%96%87&query1=Physiological%2BMechanism&&fq=dc.project.title_filter%3ATrigonobalanus%5C+doichangensis%5C+is%5C+an%5C+endangered%5C+plant.%5C+In%5C+this%5C+paper%2C%5C+the%5C+megasporogenesis%5C+and%5C+development%5C+of%5C+female%5C+gametophyte%2C%5C+seed%5C+morphological%5C+traits%5C+and%5C+seed%5C+germination%2C%5C+seed%5C+conservation%2C%5C+micropropagation%5C+and%5C+acclimatization%5C+of%5C+this%5C+species%5C+were%5C+studied.%5C+Combined%5C+with%5C+the%5C+published%5C+results%5C+of%5C+cytology%2C%5C+molecular%5C+genetics%5C+and%5C+other%5C+researches%2Cthe%5C+mechanisms%5C+of%5C+extinction%2C%5C+basic%5C+biology%5C+and%5C+technology%5C+of%5C+germplasm%5C+conservation%5C+and%5C+acclimatization%5C+of%5C+T.%5C+doichangensis%5C+were%5C+discussed.%5C+The%5C+main%5C+results%5C+are%5C+summarized%5C+as%5C+follows%5C%3A1.%5C+Megasporogenesis%5C+and%5C+development%5C+of%5C+female%5C+gametophyte%EF%BC%8CStamens%5C+exist%5C+under%5C+the%5C+stigma%5C+of%5C+T.%5C+doichangensis%2C%5C+and%5C+the%5C+pollen%5C+is%5C+aborted%5C+on%5C+the%5C+later%5C+development%5C+stage%5C+of%5C+pistil%2C%5C+therefore%2C%5C+the%5C+pistillate%5C+flower%5C+in%5C+function%5C+is%5C+hermaphrodite%5C+flower%5C+in%5C+morphology.%5C+The%5C+ovule%5C+is%5C+anatropous%2C%5C+bitegmic%5C+and%5C+crassinucellate.%5C+The%5C+primary%5C+archesporium%5C+is%5C+hypodermal%5C+and%5C+single%5C-celled%5C+and%5C+the%5C+sporogenous%5C+cell%5C+of%5C+the%5C+nucellus%5C+functions%5C+directly%5C+as%5C+a%5C+megaspore%5C+mother%5C+cell%5C+which%5C+goes%5C+meiosis%5C+to%5C+form%5C+a%5C+linear%5C+tetrad.%5C+The%5C+chalazal%5C+megaspore%5C+of%5C+the%5C+tetrad%5C+is%5C+functional.%5C+The%5C+development%5C+of%5C+embryo%5C+sac%5C+conforms%5C+to%5C+the%5C+polygonum%5C+type.%5C+There%5C+are%5C+six%5C+ovules%5C+in%5C+the%5C+ovary%5C+of%5C+T.%5C+doichangensis%2C%5C+and%5C+only%5C+one%5C+develops%5C+into%5C+a%5C+seed%5C+in%5C+normal%5C+fruits.%5C+In%5C+the%5C+process%5C+of%5C+megasporogenesis%5C+and%5C+development%5C+of%5C+female%5C+gametophyte%2C%5C+there%5C+are%5C+several%5C+links%5C+of%5C+abortion%2C%5C+and%5C+93.3%25%5C+of%5C+mature%5C+embryo%5C+sacs%5C+is%5C+aborted.2.%5C+Morphological%5C+characters%5C+and%5C+germination%5C+of%5C+seeds%EF%BC%8CMost%5C+of%5C+the%5C+variation%5C+occurred%5C+among%5C+individual%5C+trees%5C+within%5C+populations%5C+in%5C+seed%5C+morphological%5C+traits%5C+%5C%28length%2C%5C+width%5C+and%5C+1000%5C-seed%5C+weight%5C%29%5C+and%5C+germination%5C-related%5C+indices%5C+%5C%28germination%5C+percentage%2C%5C+germination%5C+index%5C+and%5C+vigor%5C+index%5C%29.%5C+In%5C+addition%2C%5C+the%5C+variation%5C+in%5C+percentage%5C+of%5C+well%5C-developed%5C+seeds%5C+among%5C+populations%5C+and%5C+among%5C+individual%5C+trees%5C+within%5C+populations%5C+is%5C+equal%2C%5C+each%5C+accounting%5C+for%5C+48%25.%5C+Each%5C+of%5C+seed%5C+morphological%5C+traits%5C+has%5C+significantly%5C+positive%5C+correlation%5C+with%5C+each%5C+other%5C+%5C%28p%5C+%3C%5C+0.01%5C%29%2C%5C+but%5C+they%5C+have%5C+no%5C+significant%5C+correlation%5C+with%5C+percentage%5C+of%5C+well%5C-developed%5C+seeds%5C+and%5C+germination%5C-related%5C+indices.%5C+In%5C+the%5C+same%5C+batch%5C+of%5C+seeds%5C+of%5C+T.%5C+doichangensis%2C%5C+there%5C+are%5C+light%5C-colored%5C+and%5C+dark%5C-colored%5C+seed%5C+coats%2C%5C+and%5C+development%5C+of%5C+light%5C-colored%5C+seeds%5C+is%5C+significantly%5C+poorer%5C+than%5C+that%5C+of%5C+dark%5C-colored%5C+seeds.The%5C+sensitivity%5C+of%5C+seeds%5C+to%5C+high%5C+temperature%5C+varys%5C+in%5C+different%5C+stages%5C+of%5C+seed%5C+imbibition.%5C+In%5C+each%5C+stage%2C%5C+heat%5C+acclimatization%5C+don%E2%80%99t%5C+increase%5C+germination%5C+percentage%2C%5C+germination%5C+index%5C+and%5C+fresh%5C+weight%5C+of%5C+seedlings.%5C+If%5C+the%5C+distilled%5C+water%5C+is%5C+substituted%5C+by%5C+solution%5C+of%5C+SA%5C+during%5C+seed%5C+imbibition%2C%5C+seed%5C+germination%5C+and%5C+germination%5C+index%5C+after%5C+heat%5C+shock%5C+are%5C+not%5C+significantly%5C+different%5C+from%5C+control%2C%5C+but%5C+they%5C+are%5C+significantly%5C+higher%5C+than%5C+that%5C+of%5C+other%5C+treatments.%5C+Moreover%2C%5C+when%5C+the%5C+seeds%5C+are%5C+treatmented%5C+with%5C+SA%2C%5C+the%5C+fresh%5C+weight%5C+of%5C+seedlings%5C+is%5C+significantly%5C+higher%5C+than%5C+that%5C+of%5C+control%5C+and%5C+other%5C+treatments.3.%5C+Seed%5C+conservation%EF%BC%8CSeeds%5C+of%5C+T.%5C+doichangensis%5C+belong%5C+to%5C+orthodox%5C+seeds%5C+which%5C+can%5C+tolerate%5C+certain%5C+level%5C+of%5C+dehydration.%5C+The%5C+condition%5C+of%5C+low%5C+temperature%5C+and%5C+low%5C+water%5C+content%5C+of%5C+seeds%5C+is%5C+conducive%5C+to%5C+seed%5C+conservation.Germination%5C+of%5C+fresh%5C+seeds%5C+shows%5C+significant%5C+variation%5C+among%5C+populations%2C%5C+howerer%2C%5C+germination%5C+of%5C+the%5C+seeds%5C+after%5C+storage%5C+for%5C+one%5C+year%5C+in%5C+ro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the industrial production of human development, environmental pollution problems have become more serious. Metal ion pollution, especially pollution of heavy metal ions, through the biological processes of crop uptaking and accumulation, causing a serious threat to food security; by affecting plant growth, resulting in ecological problems. Plants response to metal ion stress has an important scientific significance and application value. In this study, we used Columbia ecotype, mutants of Arabidopsis thaliana and Brassica napus as materials, the first part, by detecting the relationship between the changes of osmotic adjustment and the charge (or the radius) of metal ions in leaves, we explored the mechanisms of plant roots response to metal ion stress, in order to research the plants response to metal ion stress at the plant level; the second part, by detecting the changes of membrane lipid molecules in leaves and roots, we studyed the mechanism of plant cell membrane response to metal ion stress, so as to explore the plants response to metal ion stress at the cell level.The main results of the first part are as below: 1. Metal ion has effects on the surrounding molecules depends on its radius, the charge and the structure of electron shell. According to the periodic table, we selected 16 kinds of metal ions with different ionic charge, radius and electron shell structure in research. The setting of the metal ion stress was water culture system with adding metal ions, and we studyed the plants response to metal ions through the roots of and Arabidopsis thaliana and Brassica napus.We found that as the concentration of metal ion increased, the relative leakage of the leaves also increased; and the metal ion which had bigger radius resulted in bigger 50% relative leakage, such as Ca2+ stresses resulted in the biggest 50% relative leakage, K+, Na+ and Mg2+ as follows; and heavy metal ions such as Cu2+, Cd2+, Zn2+ and Hg2+ resulted in the smallest 50% relative leakage of all; and the 50% relative leakage of scarce metals Ce3+ was between them. The evidence above suggested that the relationship between the stress capacity of metal ions and its radius was inverse, and the charge of metal ions was positive, respectively. Above all, the relationship between the stress capacity of metal ions and their ionic potential (ionic charge / radius) was positive. 2. Plants could accumulate Osmo regulation substances and produce lipid peroxidation product malondialdehyde after stress condition. We found that after the same concentration of alkali metal Li+, Na+ and K+ stressed Arabidopsis thaliana and Brassica napus, Li+ stress induced the most free proline and MDA production, and the ion radius of which is the smallest; and after the same concentration of Na+, Mg2+ and Al3+ stress Arabidopsis thaliana and Brassica napus, Al3+ stress induced the most free proline and MDA production, and the ion charge of which was the most; and after the same concentration of Ca2+, Al3+and Fe3+ stress Arabidopsis thaliana and Brassica napus, Fe3+ stress induced the most free proline and MDA production. The evidence above suggested that the relationship between the capacity of proline accumulation and MDA and its radius was inverse, and the charge of metal ions was positive, respectively. Above all, the the relationship between the stress capacity of metal ions and their ionic potential (ionic charge/radius) was positive. Besides, the relationship between the production of MDA and metal ion was similar. 3. Detached leaves of plants with the metal ion treatment also accumulated proline. However, we found that the proline accumulation in the detached leaves had no relationship with the radius and the ionic charge of metal. This showed that the plants response to metal ions was conducted through the root system, rather than a direct result of cells responsed to osmotic stress. 4. To further verify the plant''s root system could sense the metal ions stress, we damaged the root of the plant to observe that metal ions had effects on the proline accumulation in the case of the injury. And we found that root with heat stress had no effect on the relationship between the metal ions and proline accumulation. However, mechanically cutting the root even part of the root of the plants, then exposured to hydroponic culture with the metal ions 5 day, we found that there was no significant diffierence between them. The evidence above suggested that the plants responsed to metal ions conducted through roots are complex, and how to quantify this effect required our futher experiment. 5. P5CS is the key enzyme in proline biothesis in plants. We compared wild-type and P5CS deficient mutants of Arabidopsis responsed to metal ion stress, and found that compared to Columbia ecotype of Arabidopsis thaliana, knockout mutations of P5CS1 resulted in less free proline production under the same stress condition. This suggested that metal ions induced proline production by increasing its synthesis rather than reducing degradation. The main results of the second part are as below: The main organs sensing the environment is cell membrane, and cells can respond to changes through the changes of membrane lipids composition in the environment. It is a common phenomenon that environmental metal ions have effects on the plants, and how to impact the changes of the lipids is a significant question, and so far, we have no idea about this. In the same stress intensity (the value of 50% relative leakage), we use of lipid genomics approaches, and comparedthe effects of 7 metal ions (Li+,Pb2+,Cu2+,Zn2+,Co2+,Fe3+ and Cd2+) with different ionic radius, charge and the electron shell structure on the cell membrane lipids of aboveground and underground parts of plants. Because of the limited time, we did preliminary analysis from the mass of lipid data. We found that effects of the metal ions on the membrane lipid molecules have a variety of performances. And data analysis is working, two examples were as follows: 1. After Li+,Pb2+,Cu2+,Zn2+ and Co2+stressed Arabidopsis thaliana for 5days, there was no significant changes in Lysophosphlipids of roots, however, the lysophospholipid molecules of leaves increased in different degrees.The evidence above indicated that the mechanisms of roots and leaves responsed to metal ions maybe different, they were direct and indirect process, respectively. 2. Fe3+ and Cd2+ are two metal ions with lager ionic potential. We found that after Fe3+ and Cd2+ stressed Arabidopsis thaliana for 12 hours, the LysoPC in roots increased more than houdred times, but Cd2+ with the same stress intensity (the value of 50% relative leakage) had no effect on the LysoPC, almostly. The evidence above suggested that the mechanisms of membrane lipids responsed to different metal ions different, and this mainly had relationship with the characters of metal ions and so 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