MD method for the precise analysis of the O-acetyl-mannan structure and disclosure of the role in the conformational stability of insulin

doi:10.1016/j.ijbiomac.2024.129944

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	MD method for the precise analysis of the O-acetyl-mannan structure and disclosure of the role in the conformational stability of insulin
	Yang, Liu; Huang, Jia; Qin, Shihui; Shao, Huiyan; Li, Yanlang; Zhou, Ying; Zi, Chengting; Hu, Jiang-Miao
	2024
发表期刊	INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
ISSN	0141-8130
卷号	263 页码:129944
摘要	Among the diversified glycan modifications, acylation is one of the most abundant. This modification could be responsible for many of the properties of glycans, such as structural stability and specificity for biological activity. To obtain better insight into the effects of acetylation of glycans on the structure and thermostability of insulin, it is critical to investigate glycans with a high degree of acetylation. An in-depth study of three functional glycans named acetyl-mannan from Dendrobium devonianum (DDAM) was conducted herein by efficient enzymatic depolymerization, and the effect of glycosidic bonds on acetylation modification sites was studied through a molecular dynamics (MD) method, as well as its positive effect on insulin secretion, glucose uptake, and the thermal stability of tertiary structures in vitro. Further study indicated that DDAMs play a hypoglycemic role by sparking the thermostability of the insulin conformation. The hypoglycemic activity displayed a positive correlation with the degree of acetylation in DDAMs. In this work, through the MD method, we confirmed the structure characteristics of DDAMs and provided accurate data support for the structure -activity relationship analysis. Thus, these findings demonstrated that DDAMs might be an exceptional leading compound for the stability of insulin drug.
DOI	10.1016/j.ijbiomac.2024.129944
WOS记录号	WOS:001224906600001
引用统计	被引频次：3[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.kib.ac.cn/handle/151853/76312
专题	中国科学院昆明植物研究所
推荐引用方式 GB/T 7714	Yang, Liu,Huang, Jia,Qin, Shihui,et al. MD method for the precise analysis of the O-acetyl-mannan structure and disclosure of the role in the conformational stability of insulin[J]. INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,2024,263:129944.
APA	Yang, Liu.,Huang, Jia.,Qin, Shihui.,Shao, Huiyan.,Li, Yanlang.,...&Hu, Jiang-Miao.(2024).MD method for the precise analysis of the O-acetyl-mannan structure and disclosure of the role in the conformational stability of insulin.INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,263,129944.
MLA	Yang, Liu,et al."MD method for the precise analysis of the O-acetyl-mannan structure and disclosure of the role in the conformational stability of insulin".INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES 263(2024):129944.

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