Bioassay-guided isolation of anti-leukemic steroids from Aglaia abbreviata by inducing apoptosis

doi:10.1016/j.bioorg.2024.107147

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	Bioassay-guided isolation of anti-leukemic steroids from Aglaia abbreviata by inducing apoptosis
	Liu, Keying; Yang, Jue; Tang, Yunyan; Li, Yanan; Hu, Zhanxing; Hao, Xiaojiang; Yi, Ping; Yuan, Chunmao
	2024
发表期刊	BIOORGANIC CHEMISTRY
ISSN	0045-2068
卷号	144 页码:107147
摘要	The strategy of bioactivity-guided isolation is widely used to obtain active compounds as quickly as possible. Thus, the inhibitory effects on human erythroleukemia cells (HEL) were applied to guide the isolation of the antileukemic compounds from Aglaia abbreviata. As a result, 19 compounds (16 steroids, two phenol derivatives, and a rare C12 chain nor-sesquiterpenoid), including 13 new compounds, were isolated and identified based on spectroscopic data analysis, single -crystal X-ray diffraction data, and electronic circular dichroism (ECD) calculations. Among them, 9 steroids exhibited good selective anti -leukemic activity against HEL and K562 (human chronic myeloid leukemia cells) cells with IC50 values between 2.29 +/- 0.18 mu M and 19.58 +/- 0.13 mu M. Notably, all the active compounds had relatively lower toxicity on the normal human liver cell line (HL -7702). Furthermore, five compounds (1, 4, 8, 10, and 19) displayed good anti-inflammatory effects, with IC50 values between 7.15 +/- 0.16 and 27.1 +/- 0.37 mu M. An alpha,beta-unsaturated ketone or a 5,6 Delta double bond was crucial for improving anti -leukemic effect from the structure-activity relationship analysis. The compound with the most potential, 14 was selected for the preliminary mechanistic study. Compound 14 can induce apoptosis and cause cell cycle arrest. The expression of the marker proteins, such as PARP and caspase 3, were notably effected by this compound, thus inducing apoptosis. In conclusion, our investigation implied that compound 14 may serve as a potential anti -leukemia agent.
DOI	10.1016/j.bioorg.2024.107147
WOS记录号	WOS:001175237300001
引用统计	被引频次：5[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.kib.ac.cn/handle/151853/76262
专题	中国科学院昆明植物研究所
推荐引用方式 GB/T 7714	Liu, Keying,Yang, Jue,Tang, Yunyan,et al. Bioassay-guided isolation of anti-leukemic steroids from Aglaia abbreviata by inducing apoptosis[J]. BIOORGANIC CHEMISTRY,2024,144:107147.
APA	Liu, Keying.,Yang, Jue.,Tang, Yunyan.,Li, Yanan.,Hu, Zhanxing.,...&Yuan, Chunmao.(2024).Bioassay-guided isolation of anti-leukemic steroids from Aglaia abbreviata by inducing apoptosis.BIOORGANIC CHEMISTRY,144,107147.
MLA	Liu, Keying,et al."Bioassay-guided isolation of anti-leukemic steroids from Aglaia abbreviata by inducing apoptosis".BIOORGANIC CHEMISTRY 144(2024):107147.

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