A polyphasic approach in the identification and biochemical characterization of Dunaliella tertiolecta with biodiesel potential from a saltern in Mauritius

doi:10.7717/peerj.18325

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	A polyphasic approach in the identification and biochemical characterization of Dunaliella tertiolecta with biodiesel potential from a saltern in Mauritius
	Ramdhony, Kamlesh; Puchooa, Daneshwar; Faraj, Turki Kh.; Alrefaei, Abdulwahed Fahad; Li, Junfu; Jeewon, Rajesh
	2024
发表期刊	PEERJ
ISSN	2167-8359
卷号	12 页码:e18325
摘要	Bioprospecting robust and oleaginous strain is crucial for the commercialization of microalgae-based biodiesel. In this study, a microalgal strain SCH18 was isolated from a solar saltern located in Mauritius. This isolate was identified as Dunaliella tertiolecta based on a polyphasic approach that combined molecular, physiological, and morphological analyses. Furthermore, the effect of different salinities on the biochemical composition and fatty acid profile of this microalga was investigated to explore its potential in producing biodiesel. Results from the growth studies showed that salinity of 1.0 M NaCl was optimal for achieving a high growth rate. Under this salt concentration, the growth rate and the doubling time were calculated as 0.39 +/- 0.003 day-1 and 1.79 +/- 0.01 days, respectively. In terms of biochemical composition, a substantial amount of carbohydrate (42.02 +/- 5.20%), moderate amount of protein (30.35 +/- 0.18%) and a low lipid content (17.81 +/- 2.4%) were obtained under optimal NaCl concentration. The fatty acid analysis indicated the presence of palmitic acid, stearic acid, palmitoleic acid, oleic acid, linoleic acid, gamma, and alpha-linolenic acids, which are suitable for biodiesel synthesis. The predicted biodiesel properties were in accordance with the standard of ASTM 6751, indicating that the microalgal isolate D. tertiolecta SCH18 is a potential candidate for use in biodiesel production.
DOI	10.7717/peerj.18325
WOS记录号	WOS:001415417400008
引用统计
文献类型	期刊论文
条目标识符	http://ir.kib.ac.cn/handle/151853/76233
专题	中国科学院昆明植物研究所
推荐引用方式 GB/T 7714	Ramdhony, Kamlesh,Puchooa, Daneshwar,Faraj, Turki Kh.,et al. A polyphasic approach in the identification and biochemical characterization of Dunaliella tertiolecta with biodiesel potential from a saltern in Mauritius[J]. PEERJ,2024,12:e18325.
APA	Ramdhony, Kamlesh,Puchooa, Daneshwar,Faraj, Turki Kh.,Alrefaei, Abdulwahed Fahad,Li, Junfu,&Jeewon, Rajesh.(2024).A polyphasic approach in the identification and biochemical characterization of Dunaliella tertiolecta with biodiesel potential from a saltern in Mauritius.PEERJ,12,e18325.
MLA	Ramdhony, Kamlesh,et al."A polyphasic approach in the identification and biochemical characterization of Dunaliella tertiolecta with biodiesel potential from a saltern in Mauritius".PEERJ 12(2024):e18325.

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