Actein ameliorates diet-induced obesity through the activation of AMPK-mediated white fat browning

doi:10.1016/j.phymed.2024.156009

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	Actein ameliorates diet-induced obesity through the activation of AMPK-mediated white fat browning
	Yuan, Jingjing; Nian, Yin; Wang, Xin; Shi, Qiangqiang; Shui, Shanshan; Cai, Hao; Lin, Yan; Zhang, Xian; Wang, Fangbin; Chen, Juan; Qiu, Minghua; Liu, Jian
	2024
发表期刊	PHYTOMEDICINE
ISSN	0944-7113
卷号	134 页码:156009
摘要	Background: Targeting white adipose tissue (WAT) browning to increase systemic energy expenditure is a promising therapeutic strategy to combat obesity. Actein from Actaea cimicifuga L. has recently been reported to ameliorate high fat-induced hepatic steatosis. However, the effect of actein on diet-induced obesity merits more and further investigation. Purpose: We aimed to examine the anti-obesity potential of actein and unravel its actions on WAT browning. Methods: The effect of actein on diet-induced obesity was evaluated using a high-fat diet model in C57BL/6 mice. Systemic energy expenditure of mice was measured with a combined indirect calorimetry system. Quantitative real-time PCR analyses were performed to investigate the mRNA levels of genes involved in thermogenesis, browning, and lipolysis. The protein levels were assessed by Western blot. Moreover, WAT explants and a transwell co-culture system consisting of SVFs and adipocytes were constructed to study the mechanisms of actein on promoting WAT browning and lipolysis. Results: At a dosage of 5 mg/kg/d, actein not only protected mice against diet-induced obesity and insulin resistance, but also reversed pre-established obesity and glucose intolerance in mice. Meanwhile, actein facilitated systemic energy expenditure by activating WAT lipolysis and browning. Further, mechanistic studies revealed that actein indirectly induced epididymal adipocyte lipolysis and directly promoted a white-to-beige conversion of subcutaneous adipocytes by activating the AMPK signaling. Conclusion: Actein ameliorated diet-induced obesity and was discovered as a natural lead compound directly targeting white-to-beige conversion of subcutaneous adipocytes, suggesting the potential of developing new therapies for obesity and associated metabolic disorders.
DOI	10.1016/j.phymed.2024.156009
WOS记录号	WOS:001313183700001
引用统计
文献类型	期刊论文
条目标识符	http://ir.kib.ac.cn/handle/151853/76063
专题	中国科学院昆明植物研究所
推荐引用方式 GB/T 7714	Yuan, Jingjing,Nian, Yin,Wang, Xin,et al. Actein ameliorates diet-induced obesity through the activation of AMPK-mediated white fat browning[J]. PHYTOMEDICINE,2024,134:156009.
APA	Yuan, Jingjing.,Nian, Yin.,Wang, Xin.,Shi, Qiangqiang.,Shui, Shanshan.,...&Liu, Jian.(2024).Actein ameliorates diet-induced obesity through the activation of AMPK-mediated white fat browning.PHYTOMEDICINE,134,156009.
MLA	Yuan, Jingjing,et al."Actein ameliorates diet-induced obesity through the activation of AMPK-mediated white fat browning".PHYTOMEDICINE 134(2024):156009.

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