Phylogenetic trends in TZ staining analysis of six deep dormancy seeds

doi:10.1017/S0960258524000060

KIB OpenIR

	Phylogenetic trends in TZ staining analysis of six deep dormancy seeds
	Peng, Chen Yin; Wu, Yu; Huang, Wen Hui; Deng, Zhi Yun; Sun, Xiao Rui; Wang, Ming Zhu; Pritchard, Hugh W.; Shen, Yong Bao; Xu, Jin Ya; Yu, Xiang Yu; Guo, Cong Cong
	2024
发表期刊	SEED SCIENCE RESEARCH
ISSN	0960-2585
卷号	34 期号:2 页码:90-102
摘要	The assessment of seed quality and physiological potential is essential in seed production and crop breeding. In the process of rapid detection of seed viability using tetrazolium (TZ) staining, it is necessary to spend a lot of labour and material resources to explore the pretreatment and staining methods of hard and solid seeds with physical barriers. This study explores the TZ staining methods of six hard seeds (Tilia miqueliana, Tilia henryana, Sassafras tzumu, Prunus subhirtella, Prunus sibirica, and Juglans mandshurica) and summarizes the TZ staining conditions required for hard seeds by combining the difference in fat content between seeds and the kinship between species, thus providing a rapid viability test method for the protection of germplasm resources of endangered plants and the optimization of seed bank construction. The TZ staining of six species of hard seeds requires a staining temperature above 35 degrees C and a TZ solution concentration higher than 1%. Endospermic seeds require shorter staining times than exalbuminous seeds. The higher the fat content of the seeds, the lower the required incubation temperature and TZ concentration for staining, and the longer the staining time. And the closer the relationship between the two species, the more similar their staining conditions become. The TZ staining method of similar species can be predicted according to the genetic distance between the phylogenetic trees, and the viability of new species can be detected quickly.
DOI	10.1017/S0960258524000060
WOS记录号	WOS:001202318400001
引用统计
文献类型	期刊论文
条目标识符	http://ir.kib.ac.cn/handle/151853/76024
专题	中国科学院昆明植物研究所
推荐引用方式 GB/T 7714	Peng, Chen Yin,Wu, Yu,Huang, Wen Hui,et al. Phylogenetic trends in TZ staining analysis of six deep dormancy seeds[J]. SEED SCIENCE RESEARCH,2024,34(2):90-102.
APA	Peng, Chen Yin.,Wu, Yu.,Huang, Wen Hui.,Deng, Zhi Yun.,Sun, Xiao Rui.,...&Guo, Cong Cong.(2024).Phylogenetic trends in TZ staining analysis of six deep dormancy seeds.SEED SCIENCE RESEARCH,34(2),90-102.
MLA	Peng, Chen Yin,et al."Phylogenetic trends in TZ staining analysis of six deep dormancy seeds".SEED SCIENCE RESEARCH 34.2(2024):90-102.

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