Orchid seeds are not always short lived in a conventional seed bank!

doi:10.1093/aob/mcae021

KIB OpenIR

	Orchid seeds are not always short lived in a conventional seed bank!
	Francisqueti, Ana Maria; Marin, Rafael Rubio; Hengling, Mariane Marangoni; Hosomi, Silverio Takao; Pritchard, Hugh W.; Custodio, Ceci Castilho; Machado-Neto, Nelson Barbosa
	2024
发表期刊	ANNALS OF BOTANY
ISSN	0305-7364
卷号	133 期号:7 页码:941-952
摘要	Background and Aims: Orchid seeds are reputed to be short lived in dry, cold storage conditions, potentially limiting the use of conventional seed banks for long-term ex situ conservation. This work explores whether Cattleya seeds are long lived or not during conventional storage (predried to similar to 12 % relative humidity, then stored at -18 degrees C).Methods: We explored the possible interaction of factors influencing seed lifespan in eight species of the genus Cattleya using physiological (germination and vigour), biochemical (gas chromatography), biophysical (differential scanning calorimetry) and morphometric methods. Seeds were desiccated to similar to 3 % moisture content and stored at -18 degrees C for more than a decade, and seed quality was measured via three in vitro germination techniques. Tetrazolium staining was also used to monitor seed viability during storage. The morphometric and germination data were subjected to ANOVA and cluster analysis, and seed lifespan was subjected to probit analysis.Key Results: Seeds of all Cattleya species were found to be desiccation tolerant, with predicted storage lifespans (P-50y) of similar to 30 years for six species and much longer for two species. Cluster analysis showed that the three species with the longest-lived seeds had smaller (9-11 %) airspaces around the embryo. The post-storage germination method impacted the quality assessment; seeds equilibrated at room temperature for 24 h or in 10 % sucrose solution had improved germination, particularly for the seeds with the smallest embryos. Chromatography revealed that the seeds of all eight species were rich in linoleic acid, and differential scanning calorimetry identified a peak that might be auxiliary to selecting long-lived seeds.Conclusions: These findings show that not all orchids produce seeds that are short lived, and our trait analyses might help to strengthen prediction of seed longevity in diverse orchid species.
DOI	10.1093/aob/mcae021
WOS记录号	WOS:001180944100001
引用统计
文献类型	期刊论文
条目标识符	http://ir.kib.ac.cn/handle/151853/75967
专题	中国科学院昆明植物研究所
推荐引用方式 GB/T 7714	Francisqueti, Ana Maria,Marin, Rafael Rubio,Hengling, Mariane Marangoni,et al. Orchid seeds are not always short lived in a conventional seed bank![J]. ANNALS OF BOTANY,2024,133(7):941-952.
APA	Francisqueti, Ana Maria.,Marin, Rafael Rubio.,Hengling, Mariane Marangoni.,Hosomi, Silverio Takao.,Pritchard, Hugh W..,...&Machado-Neto, Nelson Barbosa.(2024).Orchid seeds are not always short lived in a conventional seed bank!.ANNALS OF BOTANY,133(7),941-952.
MLA	Francisqueti, Ana Maria,et al."Orchid seeds are not always short lived in a conventional seed bank!".ANNALS OF BOTANY 133.7(2024):941-952.

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