Jasmonate/ethylene- and NaWRKY6/3-regulated Alternaria resistance depends on ethylene response factor 1B-like in Nicotiana attenuata

doi:10.1093/jxb/erae320

KIB OpenIR

	Jasmonate/ethylene- and NaWRKY6/3-regulated Alternaria resistance depends on ethylene response factor 1B-like in Nicotiana attenuata
	Ma, Lan; Song, Na; Duan, Qing; Du, Wenwen; Li, Xiang; Jia, Wenjie; Cui, Guangfen; Wang, Jihua; Wu, Jinsong
	2024
发表期刊	JOURNAL OF EXPERIMENTAL BOTANY
ISSN	0022-0957
页码	-
摘要	Biosynthesis of the phytoalexins scopoletin and scopolin in Nicotiana species is regulated by upstream signals including jasmonate (JA), ethylene (ET), and NaWRKY3 in response to the necrotrophic fungus Alternaria alternata, which causes brown spot disease. However, how these signals are coordinated to regulate these phytoalexins remains unknown. By analyzing RNA sequencing data and RNAi, we identified NaERF1B-like (NaERF1B-L) as a key player in Nicotiana attenuata during A. alternata infection by regulating the transcripts of Feruloyl-CoA 6'-hydroxylase 1 (NaF6'H1), encoding a key enzyme for scopoletin biosynthesis, and NaVS1-like (NaVS1-L), a putative biosynthetic gene of the phytoalexin solavetivone. We further demonstrated that the synergistic induction of these two genes by JA and ET signaling is mediated by NaERF1B-L. Additionally, we found that the two closely related proteins, NaWRKY6 and NaWRKY3, physically interact to enhance NaERF1B-L expression by directly binding to and activating the NaERF1B-L promoter. Collectively, our current results demonstrate that NaERF1B-L plays a positive role in resistance to A. alternata by modulating phytoalexin biosynthesis through the integration of JA/ET and NaWRKY6/3 signaling. Our findings reveal a fine-tuned transcriptional regulatory hierarchy mediated by NaERF1B-L for brown spot disease resistance in wild tobacco.Ethylene response factor NaERF1B-like integrates signals from jasmonate/ethylene and NaWRKY6/3 to regulate biosynthesis of the phytoalexin scopoletin for brown spot disease resistance to fungal pathogen Alternaria alternata in wild tobacco Nicotiana attenuata .
DOI	10.1093/jxb/erae320
WOS记录号	WOS:001297314400001
引用统计
文献类型	期刊论文
条目标识符	http://ir.kib.ac.cn/handle/151853/75945
专题	中国科学院昆明植物研究所
推荐引用方式 GB/T 7714	Ma, Lan,Song, Na,Duan, Qing,et al. Jasmonate/ethylene- and NaWRKY6/3-regulated Alternaria resistance depends on ethylene response factor 1B-like in Nicotiana attenuata[J]. JOURNAL OF EXPERIMENTAL BOTANY,2024:-.
APA	Ma, Lan.,Song, Na.,Duan, Qing.,Du, Wenwen.,Li, Xiang.,...&Wu, Jinsong.(2024).Jasmonate/ethylene- and NaWRKY6/3-regulated Alternaria resistance depends on ethylene response factor 1B-like in Nicotiana attenuata.JOURNAL OF EXPERIMENTAL BOTANY,-.
MLA	Ma, Lan,et al."Jasmonate/ethylene- and NaWRKY6/3-regulated Alternaria resistance depends on ethylene response factor 1B-like in Nicotiana attenuata".JOURNAL OF EXPERIMENTAL BOTANY (2024):-.

条目包含的文件		下载所有文件
文件名称/大小	文献类型	版本类型	开放类型	使用许可
10.1093_jxb_erae320.（7497KB）	期刊论文	出版稿	开放获取	CC BY-NC-SA	浏览下载