A new genus of alphasatellites associated with banana bunchy top virus in Southeast Asia

doi:10.1093/ve/vead076

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	A new genus of alphasatellites associated with banana bunchy top virus in Southeast Asia
	Guyot, Valentin; Trieu, Tien-Dung; Insisiengmay, Oudomphone; Zhang, Ting; Iskra-Caruana, Marie-Line; Pooggin, Mikhail M.
	2024
发表期刊	VIRUS EVOLUTION
卷号	10 期号:1 页码:vead076
摘要	Autonomously replicating alphasatellites (family Alphasatellitidae) are frequently associated with plant single-stranded (ss)DNA viruses of the families Geminiviridae, Metaxyviridae, and Nanoviridae. Alphasatellites encode a single replication-initiator protein (Rep) similar to Rep proteins of helper viruses and depend on helper viruses for encapsidation, movement, and transmission. Costs versus benefits of alphasatellite-helper virus association are poorly understood. Our surveys in Southeast Asia (SEA) for wild and cultivated banana plants infected with banana bunchy top virus (BBTV, Nanoviridae) and Illumina sequencing reconstruction of their viromes revealed, in addition to a six-component BBTV genome, one to three distinct alphasatellites present in sixteen of twenty-four BBTV-infected plants. Comparative nucleotide and Rep protein sequence analyses classified these alphasatellites into four distinct species: two known species falling into the genus Muscarsatellite (subfamily Petromoalphasatellitinae) previously identified in SEA and two novel species falling into the tentative genus Banaphisatellite (subfamily Nanoalphasatellitinae) so far containing a single species recently identified in Africa. The banaphisatellites were found to be most related to members of the genus Fabenesatellite of subfamily Nanoalphasatellitinae and the genus Gosmusatellite of subfamily Geminialphasatellitinae, both infecting dicots. This suggests a dicot origin of banaphisatellites that got independently associated with distinct strains of monocot-infecting BBTV in Africa and SEA. Analysis of conserved sequence motifs in the common regions driving replication and gene expression of alphasatellites and BBTV strains revealed both differences and similarities, pointing at their ongoing co-evolution. An impact of alphasatellites on BBTV infection and evasion of RNA interference-based antiviral defences was evaluated by measuring relative abundance of BBTV genome components and alphasatellites and by profiling BBTV- and alphasatellite-derived small interfering RNAs. Taken together, our findings shed new light on the provenance of alphasatellites, their co-evolution with helper viruses, and potential mutual benefits of their association.
DOI	10.1093/ve/vead076
WOS记录号	WOS:001159545700001
引用统计
文献类型	期刊论文
条目标识符	http://ir.kib.ac.cn/handle/151853/75937
专题	中国科学院昆明植物研究所
推荐引用方式 GB/T 7714	Guyot, Valentin,Trieu, Tien-Dung,Insisiengmay, Oudomphone,et al. A new genus of alphasatellites associated with banana bunchy top virus in Southeast Asia[J]. VIRUS EVOLUTION,2024,10(1):vead076.
APA	Guyot, Valentin,Trieu, Tien-Dung,Insisiengmay, Oudomphone,Zhang, Ting,Iskra-Caruana, Marie-Line,&Pooggin, Mikhail M..(2024).A new genus of alphasatellites associated with banana bunchy top virus in Southeast Asia.VIRUS EVOLUTION,10(1),vead076.
MLA	Guyot, Valentin,et al."A new genus of alphasatellites associated with banana bunchy top virus in Southeast Asia".VIRUS EVOLUTION 10.1(2024):vead076.

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