Genomic analyses provide insights into sex differentiation of tetraploid strawberry (Fragaria moupinensis)

doi:10.1111/pbi.14286

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	Genomic analyses provide insights into sex differentiation of tetraploid strawberry (Fragaria moupinensis)
	Qiao, Qin; Cao, Qiang; Zhang, Rengang; Wu, Mingzhao; Zheng, Yibingyue; Xue, Li; Lei, Jiajun; Sun, Hang; Liston, Aaron; Zhang, Ticao
	2024
发表期刊	PLANT BIOTECHNOLOGY JOURNAL
ISSN	1467-7644
卷号	22 期号:6 页码:1552-1565
摘要	The strawberry genus, Fragaria, exhibits a wide range of sexual systems and natural ploidy variation. Nearly, all polyploid strawberry species exhibit separate sexes (dioecy). Research has identified the sex-determining sequences as roughly conserved but with repeatedly changed genomic locations across octoploid strawberries. However, it remains unclear whether tetraploid wild strawberries evolved dioecy independently or shared a common origin with octoploid strawberries. In this study, we investigated the sex determinants of F. moupinensis, a dioecious plant with heterogametic females (ZW). Utilizing a combination of haplotype-resolved genome sequencing of the female F. moupinensis, k-mer-based and coverage-based genome-wide association studies (GWAS), and transcriptomic analysis, we discovered a non-recombining, approximately 33.6 kb W-specific region on chromosome 2a. Within this region, only one candidate sex-determining gene (FmoAFT) was identified. Furthermore, an extensive resequencing of the entire Fragaria genus indicated that the W-specific region displays conservative female specificity across all tetraploid species. This observation suggests that dioecy evolved independently in tetraploid and octoploid strawberries. Moreover, employing virus-induced gene silencing (VIGS), we knocked down the expression of the FmoAFT homologue transcript in cultivated strawberries, revealing its potential role in promoting female functions during early carpel development. We also applied DNA affinity purification sequencing (DAP-seq) and yeast one-hybrid assays to identify potential direct targets of FmoAFT. These insights shed new light on the genetic basis and evolutionary history of sex determination in strawberries, thereby facilitating the formulation of strategies to manipulate sex determination in breeding programs.
DOI	10.1111/pbi.14286
WOS记录号	WOS:001137218800001
引用统计	被引频次：5[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.kib.ac.cn/handle/151853/75917
专题	中国科学院昆明植物研究所
推荐引用方式 GB/T 7714	Qiao, Qin,Cao, Qiang,Zhang, Rengang,et al. Genomic analyses provide insights into sex differentiation of tetraploid strawberry (Fragaria moupinensis)[J]. PLANT BIOTECHNOLOGY JOURNAL,2024,22(6):1552-1565.
APA	Qiao, Qin.,Cao, Qiang.,Zhang, Rengang.,Wu, Mingzhao.,Zheng, Yibingyue.,...&Zhang, Ticao.(2024).Genomic analyses provide insights into sex differentiation of tetraploid strawberry (Fragaria moupinensis).PLANT BIOTECHNOLOGY JOURNAL,22(6),1552-1565.
MLA	Qiao, Qin,et al."Genomic analyses provide insights into sex differentiation of tetraploid strawberry (Fragaria moupinensis)".PLANT BIOTECHNOLOGY JOURNAL 22.6(2024):1552-1565.

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