Development of polymorphic microsatellite markers for distylous-homostylous Primula secundiflora (Primulaceae) using HiSeq sequencing

doi:10.1266/ggs.23-00340

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	Development of polymorphic microsatellite markers for distylous-homostylous Primula secundiflora (Primulaceae) using HiSeq sequencing
	Sun, Hua-Ying; Zhang, Wen-Ping; Zhou, Wei; Wu, Zhi-Kun; Zheng, Lan-Ping
	2024
发表期刊	GENES & GENETIC SYSTEMS
ISSN	1341-7568
卷号	99
摘要	Primula secundiflora is an insect-pollinated, perennial herb belonging to section Proliferae (Primulaceae) and exhibits considerable variation in mating system with predominantly outcrossing populations comprising long-styled and short-styled floral morphs and selfing populations comprising only homostyles. To facilitate future investigations of the population genetics and mating patterns of this species, we developed 25 microsatellite markers from P. secundiflora using next -generation sequencing and measured polymorphism and genetic diversity in a sample of 30 individuals from three natural populations. The markers displayed relatively high polymorphism, with the number of observed alleles per locus ranging from three to 16 (mean = 8.36). The observed and expected heterozygosities ranged from 0.100 to 1.000 and 0.145 to 0.843, respectively. Twenty-one of the loci were also successfully amplified in P. denticulata. These microsatellite markers could provide powerful tools for investigating patterns of population genetic diversity and the evolutionary relationships between distyly and homostyly in this species.
DOI	10.1266/ggs.23-00340
WOS记录号	WOS:001194708900001
引用统计
文献类型	期刊论文
条目标识符	http://ir.kib.ac.cn/handle/151853/75847
专题	中国科学院昆明植物研究所
推荐引用方式 GB/T 7714	Sun, Hua-Ying,Zhang, Wen-Ping,Zhou, Wei,et al. Development of polymorphic microsatellite markers for distylous-homostylous Primula secundiflora (Primulaceae) using HiSeq sequencing[J]. GENES & GENETIC SYSTEMS,2024,99.
APA	Sun, Hua-Ying,Zhang, Wen-Ping,Zhou, Wei,Wu, Zhi-Kun,&Zheng, Lan-Ping.(2024).Development of polymorphic microsatellite markers for distylous-homostylous Primula secundiflora (Primulaceae) using HiSeq sequencing.GENES & GENETIC SYSTEMS,99.
MLA	Sun, Hua-Ying,et al."Development of polymorphic microsatellite markers for distylous-homostylous Primula secundiflora (Primulaceae) using HiSeq sequencing".GENES & GENETIC SYSTEMS 99(2024).

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