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| Functional analysis of CYP71AV1 reveals the evolutionary landscape of artemisinin biosynthesis | |
| Chen, Fang-Yan; Mu, Qiu-Yan; Xu, Bing-Yi; Lei, Yu-Chen; Liu, Hui-Ying; Fang, Xin | |
| 2024 | |
| 发表期刊 | FRONTIERS IN PLANT SCIENCE
 |
| ISSN | 1664-462X |
| 卷号 | 15页码:1361959 |
| 摘要 | Artemisinin biosynthesis, unique to Artemisia annua, is suggested to have evolved from the ancestral costunolide biosynthetic pathway commonly found in the Asteraceae family. However, the evolutionary landscape of this process is not fully understood. The first oxidase in artemisinin biosynthesis, CYP71AV1, also known as amorpha-4,11-diene oxidase (AMO), has specialized from ancestral germacrene A oxidases (GAOs). Unlike GAO, which exhibits catalytic promiscuity toward amorpha-4,11-diene, the natural substrate of AMO, AMO has lost its ancestral activity on germacrene A. Previous studies have suggested that the loss of the GAO copy in A. annua is responsible for the abolishment of the costunolide pathway. In the genome of A. annua, there are two copies of AMO, each of which has been reported to be responsible for the different product profiles of high- and low-artemisinin production chemotypes. Through analysis of their tissue-specific expression and comparison of their sequences with those of other GAOs, it was discovered that one copy of AMO (AMOHAP) exhibits a different transcript compared to the reported artemisinin biosynthetic genes and shows more sequence similarity to other GAOs in the catalytic regions. Furthermore, in a subsequent in vitro enzymatic assay, the recombinant protein of AMOHAP unequivocally demonstrated GAO activity. This result clearly indicates that AMOHAP is a GAO rather than an AMO and that its promiscuous activity on amorpha-4,11-diene has led to its misidentification as an AMO in previous studies. In addition, the divergent expression pattern of AMOHAP compared to that of the upstream germacrene A synthase may have contributed to the abolishment of costunolide biosynthesis in A. annua. Our findings reveal a complex evolutionary landscape in which the emergence of a new metabolic pathway replaces an ancestral one. |
| DOI | 10.3389/fpls.2024.1361959 |
| WOS记录号 | WOS:001196176100001 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.kib.ac.cn/handle/151853/75828 |
| 专题 | 中国科学院昆明植物研究所 |
| 推荐引用方式 GB/T 7714 | Chen, Fang-Yan,Mu, Qiu-Yan,Xu, Bing-Yi,et al. Functional analysis of CYP71AV1 reveals the evolutionary landscape of artemisinin biosynthesis[J]. FRONTIERS IN PLANT SCIENCE,2024,15:1361959. |
| APA | Chen, Fang-Yan,Mu, Qiu-Yan,Xu, Bing-Yi,Lei, Yu-Chen,Liu, Hui-Ying,&Fang, Xin.(2024).Functional analysis of CYP71AV1 reveals the evolutionary landscape of artemisinin biosynthesis.FRONTIERS IN PLANT SCIENCE,15,1361959. |
| MLA | Chen, Fang-Yan,et al."Functional analysis of CYP71AV1 reveals the evolutionary landscape of artemisinin biosynthesis".FRONTIERS IN PLANT SCIENCE 15(2024):1361959. |
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| 文件名称/大小 | 文献类型 | 版本类型 | 开放类型 | 使用许可 | ||
| 10.3389_fpls.2024.13（1164KB） | 期刊论文 | 出版稿 | 开放获取 | CC BY-NC-SA | 浏览 请求全文 | |
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