Urea-Induced Enhancement of Hypocrellin A Synthesis in Shiraia bambusicola GDMCC 60438: Strategies and Mechanisms

doi:10.3390/fermentation10080381

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	Urea-Induced Enhancement of Hypocrellin A Synthesis in Shiraia bambusicola GDMCC 60438: Strategies and Mechanisms
	Tang, Yanbo; Wen, Yongdi; Zhang, Xiang; Gao, Qian; Yu, Fuqiang; Wu, Zhenqiang; Tian, Xiaofei
	2024
发表期刊	FERMENTATION-BASEL
卷号	10 期号:8 页码:381
摘要	Hypocrellin A (HA) is a valuable pigment with promising applications in biotechnology and pharmaceuticals. The submerged cultivation of Shiraia bambusicola offers a strategic opportunity to enhance HA production. This study investigates the regulatory mechanisms for HA biosynthesis through urea supplementation and presents a strategy to increase HA yield. In the absence of urea, S. bambusicola (GDMCC 60438) does not synthesize HA. However, the addition of 40 g/L urea 12 h into the fermentation process results in a final HA production of 46.7 +/- 8.2 mg/L. Morphological analysis reveals an optimized environment for HA synthesis, characterized by a densely intertwined and reticular hyphal structure with minute pores. RNA sequencing shows significant upregulation of genes involved in DNA repair, recombination, and metabolism. Conversely, genes related to cellular homeostasis, cell-wall chitin, and amino polysaccharide metabolism are downregulated. Urea supplementation facilitates the upregulation of amino acid metabolism and the cysteine desulfurase gene, enhancing acetyl-CoA accumulation within the mycelium and providing the necessary precursor materials for HA synthesis. Our work underscores the pivotal role of urea in regulating HA biosynthesis and proposes a practical approach to enhance HA production. The findings contribute novel insights to the fields of biotechnology for pharmaceuticals.
DOI	10.3390/fermentation10080381
WOS记录号	WOS:001306867000001
引用统计
文献类型	期刊论文
条目标识符	http://ir.kib.ac.cn/handle/151853/75806
专题	中国科学院昆明植物研究所
推荐引用方式 GB/T 7714	Tang, Yanbo,Wen, Yongdi,Zhang, Xiang,et al. Urea-Induced Enhancement of Hypocrellin A Synthesis in Shiraia bambusicola GDMCC 60438: Strategies and Mechanisms[J]. FERMENTATION-BASEL,2024,10(8):381.
APA	Tang, Yanbo.,Wen, Yongdi.,Zhang, Xiang.,Gao, Qian.,Yu, Fuqiang.,...&Tian, Xiaofei.(2024).Urea-Induced Enhancement of Hypocrellin A Synthesis in Shiraia bambusicola GDMCC 60438: Strategies and Mechanisms.FERMENTATION-BASEL,10(8),381.
MLA	Tang, Yanbo,et al."Urea-Induced Enhancement of Hypocrellin A Synthesis in Shiraia bambusicola GDMCC 60438: Strategies and Mechanisms".FERMENTATION-BASEL 10.8(2024):381.

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